植物微丝骨架的研究进展
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摘要
微丝骨架是细胞骨架的重要组成部分,在各种细胞活动中都发挥着重要作用。微丝骨架的主要组成部分是肌动蛋白和肌动蛋白结合蛋白,参与细胞形态建成、物质运输和信号转导等生命活动。通过鬼笔环肽标记或表达荧光融合蛋白等方法,国内外许多学者对植物微丝骨架的组成、功能等进行了大量的研究,并取得了一些成果。基于前人的研究,本研究从组成、功能及研究方法三个方面对植物微丝骨架的进行概述。
Microfilament,the main ingredient of the cytoskeleton,plays an important role in various life activities of cell. Microfilament is comprised of actin and actin binding proteins,and takes part in life activities such as cell morphology,material transportation and signal transduction. Using the methods of phalloidin labeling or expressing fusion protein with fluorescence,extensive researches on the component,function about the plant microfilament have been done and some achievements have been obtained. Based on these previous studies,the recent research advances on component and function of the plant microfilament as well as the related investigated methods were summarized.
Microfilament,the main ingredient of the cytoskeleton,plays an important role in various life activities of cell. Microfilament is comprised of actin and actin binding proteins,and takes part in life activities such as cell morphology,material transportation and signal transduction. Using the methods of phalloidin labeling or expressing fusion protein with fluorescence,extensive researches on the component,function about the plant microfilament have been done and some achievements have been obtained. Based on these previous studies,the recent research advances on component and function of the plant microfilament as well as the related investigated methods were summarized.
引文
Aizawa H.,Sameshima M.,and Yahara I.,1997,A green fluores cent protein-actin fusion protein dominantly inhibits cytoki nesis,cell spreading,and locomotion in Dictyostelium,Cel Structure and Function,22(3):335-345
Cárdenas L.,Lovy-Wheeler A.,Wilsen K.L.,and Hepler P.K.2005,Actin polymerization promotes the reversal of stream ing in the apex of pollen tubes,Cell Motility and the Cy toskeleton,61(2):112-127
Chen Q.,Huang S.J.,and Yu R.,2011,Regulation of plant Actin dynamics,Zhiwu Shengli Xuebao(Plant Physiology Journal)47(1):18-26(陈琼,黄善金,于荣,2011,植物微丝骨架动态变化的调节,植物生理学报,47(1):18-26)
Dos Remedios C.G.,Chhabra D.,Kekic M.,Kekic M.,Dedova IV.,Tsubakihara M.,Berry D.A.,and Nosworthy N.J.,2003Actin binding proteins:regulation of cytoskeletal microfila ments,Physiological Reviews,83(2):433-473
Fan T.T.,2012,Study on the physiological function of Arabidopsi actin binding protein Profilin3 in vivo,Dissertation for Ph.DLanzhou University,Supervisor:An L.Z.,pp.9-33(樊婷婷2012,拟南芥微丝结合蛋白Profilin3体内生理功能研究,博士学位论文,兰州大学,导师:安黎哲,pp.9-33)
He X.,Liu Y.M.,Wang W.,and Li Y.,2006,Distribution of G-actin is related to root hair growth of wheat,Annals of Botany,98(1):49-55
Higaki T.,Sano T.,and Hasezawa S.,2007,Actin microfilamen dynamics and actin side-binding proteins in plants,Curren Opinion in Plant Biology,10(6):549-556
Jian L.C.,1991,Plant cytoskeleton,Zhiwuxue Tongbao(Chinese Bulletin of Botany),8(3):1-13(简令成,1991,植物细胞骨架,植物学通报,8(3):1-13)
Ketelaar T.,2013,The actin cytoskeleton in root hairs:all is fin at the tip,Current Opinion in Plant Biology,16(6):749-756
Ketelaar T.,Anthony R.G.,and Hussey P.J.,2004,Green fluores cent protein-m Talin causes defects in actin organization and cell expansion in Arabidopsis and inhibits actin depolymer izing factor's actin depolymerizing activity in vitro,Plan Physiology,136(4):3990-3998
Kost B.,Spielhofer P.,and Chua N.H.,1998,A GFP-mouse talin fusion protein labels plant actin filamentsin vivoand visual izes the actin cytoskeleton in growing pollen tubes,Th Plant Journal,16(3):393-401
Lei Y.H.,Yan Z.F.,Yan Y.P.,and Wei J.K.,2000,A brife sketch o the progress of microfilament cytoskeleton and signal trans duction,Huabei Nongxuebao(Acta Agriculture Boreall-Sini ca),15(1):37-41(雷宇华,闫芝芬,严玉平,魏建坤,2000微丝骨架与信号转导研究进展,华北农学报,15(1):37-41)
Li X.B.,Fan X.P.,Wang X.L.,Cai L.,and Yang W.C.,2005The cotton ACTIN1 gene is functionally expressed in fiber and participates in fiber elongation,The Plant Cell,17859-875
Li Y.,Zee S.Y.,Liu Y.M.,Huang B.Q.,and Yen L.F.,2001,Cir cular F-actin bundles and a G-actin gradient in pollen and pollen tubes of Lilium davidii,Planta,213(5):722-730
Liu M.,and Hasenstein K.H.,2005,La3+uptake and its effect on the cytoskeleton in root protoplasts of Zea mays L,Planta220(5):658-666
Ma L.J.,ed.,2006,Xibao Shengwuxue,Xibei Nonglindaxue Press Xianyang,China,pp.155-158(马翎健,主编,2006,细胞生物学,西北农林科技大学出版社,中国,咸阳,pp.155-158)
Ma L.Z.,and Yang Y.W.,2003,Process of cellular skeleton system Neimenggu Nongyedaxue Xuebao(Journal of Inner Mongoli Institute of Agriculture and Animal Husbandry),24(2):114-116(马莉贞,杨元武,2003,细胞骨架系统的研究进展,内蒙古农业大学学报,自然科学版,24(2):114-116)
Mathur J.,2004,Cell shape development in plants,Trends in Plant Science,9(12):583-590
Mathur J.,and Hülskamp M.,2002,Microtubules and microfila ments in cell morphogenesis in higher plants,Current Biolo gy,12(19):R669-R676
Mc Elory D.,Rothenberg M.,Reece K.S.,and Wu R.,1990Characterization of the rice(Oryza sativa)actin gene family Plant Molecular Biology,15:257-268
Miao L.,2007,Recent progresses on the cellular motility,cel migratin and cytoskeleton,Shengwuwuli Xuebao(Acta Bio physica Sinica),23(4):281-289(苗龙,2007,细胞运动,细胞迁移与细胞骨架研究进展,生物物理学报,23(4):281-289)
Mullins R.D.,and Hansen S.D.,2013,In vitro studies of actin fil ament and network dynamics,Current Opinion in Cell Biol ogy,25(1):6-13
Nishimura T.,Yokota E.,Wada T.,Shimmen T.,and Okada K.2003,An Arabidopsis ACT2 dominant-negative mutation which disturbs F-actin polymerization,reveals its distinctiv function in root development,Plant and Cell Physiology,44(11):1131-1140
Sano T.,Higaki T.,Oda Y.,Hayashi T.,and Hasezawa S.,2005Appearance of actin microfilament‘twin peaks’in mitosi and their function in cell plate formation,as visualized in to bacco BY-2 cells expressing GFP-fimbrin,The Plant Jour nal,44(4):595-605
Schmit A.C.,and Lambert A.M.,1990,Microinjected fluorescen phalloidin in vivo reveals the F-actin dynamics and assembl in higher plant mitotic cells,The Plant Cell,2(2):129-138
Shi M.,Xie Y.R.,Zheng Y.Y.,Wang J.M.,Su Y.,Yang Q.Y.and Huang A.J.,2013,Oryza sativa actin-interacting protein1 is required for rice growth by promoting actin turnover The Plant Journal,73(5):747-760
Smith L.G.,2003,Cytoskeletal control of plant cell shape:getting the fine points,Current Opinion in Plant Biology,6(1):63-73
Sonesson A.,and Widell S.,1993,Cytoskeleton components o inside-out and right-side-out plasma membrane vesicle from plants,Protoplasma,177(1-2):45-52
Staiger C.J.,and Schliwa M.,1987,Actin localization and func tion in higher plants,Protoplasma,141(1):1-12
Voigt B.,Timmers A.C.J.,Samaj J.,Muller J.,Baluska F.,and Menzel D.,2005,GFP-FABD2 fusion construct allows in vivo visualization of the dynamic actin cytoskeleton in al cells of Arabidopsis seedlings,European Journal of Cell Bi ology,84(6):595-608
Wang L.,Liu Y.M.,and Li Y.,2005,Comparison of F-actin fluo rescent labeling methods in pollen tubes of Lilium davidii Plant Cell Reports,24(5):266-270
Woodhouse F.G.,and Goldstein R.E.,2013,Cytoplasmic stream ing in plant cells emerges naturally by microfilament self-or ganization,Proceedings of the National Academy of Sci ences,110(35):14132-14137
Wyatt S.E.,and Carpita N.C.,1993,The plant cytoskeleton-cell-wal continuum,Trends in Cell Biology,3(12):413-417
Xue X.H.,Zhang X.Y.,Zheng D.,and Ren X.Y.,2010,Experi mental study of the three dimensional and dynamic organi zation of actin cytoskeleton in plant cells with laser scanning confocal microscope,Xiandai Yiqi(Modern Instruments)(5):50-54(薛秀花,张晓嫣,郑东,任海云,2010,激光共聚焦显微成像技术在植物细胞微丝骨架三维动态观察中的应用,现代仪器,(5):50-54)
Ye L.F.,Liu C.K.,Meng C.M.,and Hong J.,2010,Microfilament cy toskeletons in BY-2 protoplasts and broad bean mesophyl protoplasts and the effect of latrunculin B on their distribution Zhejiangdaxue Xuebao:Nongye Yu Shengmingkexue Ban(Journal of Zhejiang University:Agriculture and Life Sci ences),36(5):497-502(叶露飞,刘成科,孟春梅,洪健,2010烟草BY-2细胞和蚕豆叶肉细胞原生质体微丝骨架及la trunculin B处理对其分布的影响,浙江大学学报:农业与生命科学版,36(5):497-502)
Zhang D.,Du Q.,Xu B.,Zhang Z.,and Li B.,2010,The actin multigene family in Populus:organization,expression and phylogenetic analysis,Mol.Genet.Genomics,284:105-119
Zhang M.B.,and Wang X.J.,2004,Morphogenesis of plant cell Xibei Zhiwu Xuebao(Acta Botanica Boreail-Occidentalia Sinica),24(1):154-160(张妙彬和王小菁,2004,植物细胞形态构建,西北植物学报,24(1):154-160)
Zhang X.T.,2012,Molecular cloning and functional analysis o actin-binding protein gene(Gh VLN1)in cotton,Thesis fo M.S.,Nanjing Agricultural University,Supervisor:Liu Kpp.9-12(张晓彤,2012,棉花Actin结合蛋白基因(Gh VLN1的克隆与功能分析,硕士学位论文,南京农业大学,导师刘康,pp.9-12)
Cárdenas L.,Lovy-Wheeler A.,Wilsen K.L.,and Hepler P.K.2005,Actin polymerization promotes the reversal of stream ing in the apex of pollen tubes,Cell Motility and the Cy toskeleton,61(2):112-127
Chen Q.,Huang S.J.,and Yu R.,2011,Regulation of plant Actin dynamics,Zhiwu Shengli Xuebao(Plant Physiology Journal)47(1):18-26(陈琼,黄善金,于荣,2011,植物微丝骨架动态变化的调节,植物生理学报,47(1):18-26)
Dos Remedios C.G.,Chhabra D.,Kekic M.,Kekic M.,Dedova IV.,Tsubakihara M.,Berry D.A.,and Nosworthy N.J.,2003Actin binding proteins:regulation of cytoskeletal microfila ments,Physiological Reviews,83(2):433-473
Fan T.T.,2012,Study on the physiological function of Arabidopsi actin binding protein Profilin3 in vivo,Dissertation for Ph.DLanzhou University,Supervisor:An L.Z.,pp.9-33(樊婷婷2012,拟南芥微丝结合蛋白Profilin3体内生理功能研究,博士学位论文,兰州大学,导师:安黎哲,pp.9-33)
He X.,Liu Y.M.,Wang W.,and Li Y.,2006,Distribution of G-actin is related to root hair growth of wheat,Annals of Botany,98(1):49-55
Higaki T.,Sano T.,and Hasezawa S.,2007,Actin microfilamen dynamics and actin side-binding proteins in plants,Curren Opinion in Plant Biology,10(6):549-556
Jian L.C.,1991,Plant cytoskeleton,Zhiwuxue Tongbao(Chinese Bulletin of Botany),8(3):1-13(简令成,1991,植物细胞骨架,植物学通报,8(3):1-13)
Ketelaar T.,2013,The actin cytoskeleton in root hairs:all is fin at the tip,Current Opinion in Plant Biology,16(6):749-756
Ketelaar T.,Anthony R.G.,and Hussey P.J.,2004,Green fluores cent protein-m Talin causes defects in actin organization and cell expansion in Arabidopsis and inhibits actin depolymer izing factor's actin depolymerizing activity in vitro,Plan Physiology,136(4):3990-3998
Kost B.,Spielhofer P.,and Chua N.H.,1998,A GFP-mouse talin fusion protein labels plant actin filamentsin vivoand visual izes the actin cytoskeleton in growing pollen tubes,Th Plant Journal,16(3):393-401
Lei Y.H.,Yan Z.F.,Yan Y.P.,and Wei J.K.,2000,A brife sketch o the progress of microfilament cytoskeleton and signal trans duction,Huabei Nongxuebao(Acta Agriculture Boreall-Sini ca),15(1):37-41(雷宇华,闫芝芬,严玉平,魏建坤,2000微丝骨架与信号转导研究进展,华北农学报,15(1):37-41)
Li X.B.,Fan X.P.,Wang X.L.,Cai L.,and Yang W.C.,2005The cotton ACTIN1 gene is functionally expressed in fiber and participates in fiber elongation,The Plant Cell,17859-875
Li Y.,Zee S.Y.,Liu Y.M.,Huang B.Q.,and Yen L.F.,2001,Cir cular F-actin bundles and a G-actin gradient in pollen and pollen tubes of Lilium davidii,Planta,213(5):722-730
Liu M.,and Hasenstein K.H.,2005,La3+uptake and its effect on the cytoskeleton in root protoplasts of Zea mays L,Planta220(5):658-666
Ma L.J.,ed.,2006,Xibao Shengwuxue,Xibei Nonglindaxue Press Xianyang,China,pp.155-158(马翎健,主编,2006,细胞生物学,西北农林科技大学出版社,中国,咸阳,pp.155-158)
Ma L.Z.,and Yang Y.W.,2003,Process of cellular skeleton system Neimenggu Nongyedaxue Xuebao(Journal of Inner Mongoli Institute of Agriculture and Animal Husbandry),24(2):114-116(马莉贞,杨元武,2003,细胞骨架系统的研究进展,内蒙古农业大学学报,自然科学版,24(2):114-116)
Mathur J.,2004,Cell shape development in plants,Trends in Plant Science,9(12):583-590
Mathur J.,and Hülskamp M.,2002,Microtubules and microfila ments in cell morphogenesis in higher plants,Current Biolo gy,12(19):R669-R676
Mc Elory D.,Rothenberg M.,Reece K.S.,and Wu R.,1990Characterization of the rice(Oryza sativa)actin gene family Plant Molecular Biology,15:257-268
Miao L.,2007,Recent progresses on the cellular motility,cel migratin and cytoskeleton,Shengwuwuli Xuebao(Acta Bio physica Sinica),23(4):281-289(苗龙,2007,细胞运动,细胞迁移与细胞骨架研究进展,生物物理学报,23(4):281-289)
Mullins R.D.,and Hansen S.D.,2013,In vitro studies of actin fil ament and network dynamics,Current Opinion in Cell Biol ogy,25(1):6-13
Nishimura T.,Yokota E.,Wada T.,Shimmen T.,and Okada K.2003,An Arabidopsis ACT2 dominant-negative mutation which disturbs F-actin polymerization,reveals its distinctiv function in root development,Plant and Cell Physiology,44(11):1131-1140
Sano T.,Higaki T.,Oda Y.,Hayashi T.,and Hasezawa S.,2005Appearance of actin microfilament‘twin peaks’in mitosi and their function in cell plate formation,as visualized in to bacco BY-2 cells expressing GFP-fimbrin,The Plant Jour nal,44(4):595-605
Schmit A.C.,and Lambert A.M.,1990,Microinjected fluorescen phalloidin in vivo reveals the F-actin dynamics and assembl in higher plant mitotic cells,The Plant Cell,2(2):129-138
Shi M.,Xie Y.R.,Zheng Y.Y.,Wang J.M.,Su Y.,Yang Q.Y.and Huang A.J.,2013,Oryza sativa actin-interacting protein1 is required for rice growth by promoting actin turnover The Plant Journal,73(5):747-760
Smith L.G.,2003,Cytoskeletal control of plant cell shape:getting the fine points,Current Opinion in Plant Biology,6(1):63-73
Sonesson A.,and Widell S.,1993,Cytoskeleton components o inside-out and right-side-out plasma membrane vesicle from plants,Protoplasma,177(1-2):45-52
Staiger C.J.,and Schliwa M.,1987,Actin localization and func tion in higher plants,Protoplasma,141(1):1-12
Voigt B.,Timmers A.C.J.,Samaj J.,Muller J.,Baluska F.,and Menzel D.,2005,GFP-FABD2 fusion construct allows in vivo visualization of the dynamic actin cytoskeleton in al cells of Arabidopsis seedlings,European Journal of Cell Bi ology,84(6):595-608
Wang L.,Liu Y.M.,and Li Y.,2005,Comparison of F-actin fluo rescent labeling methods in pollen tubes of Lilium davidii Plant Cell Reports,24(5):266-270
Woodhouse F.G.,and Goldstein R.E.,2013,Cytoplasmic stream ing in plant cells emerges naturally by microfilament self-or ganization,Proceedings of the National Academy of Sci ences,110(35):14132-14137
Wyatt S.E.,and Carpita N.C.,1993,The plant cytoskeleton-cell-wal continuum,Trends in Cell Biology,3(12):413-417
Xue X.H.,Zhang X.Y.,Zheng D.,and Ren X.Y.,2010,Experi mental study of the three dimensional and dynamic organi zation of actin cytoskeleton in plant cells with laser scanning confocal microscope,Xiandai Yiqi(Modern Instruments)(5):50-54(薛秀花,张晓嫣,郑东,任海云,2010,激光共聚焦显微成像技术在植物细胞微丝骨架三维动态观察中的应用,现代仪器,(5):50-54)
Ye L.F.,Liu C.K.,Meng C.M.,and Hong J.,2010,Microfilament cy toskeletons in BY-2 protoplasts and broad bean mesophyl protoplasts and the effect of latrunculin B on their distribution Zhejiangdaxue Xuebao:Nongye Yu Shengmingkexue Ban(Journal of Zhejiang University:Agriculture and Life Sci ences),36(5):497-502(叶露飞,刘成科,孟春梅,洪健,2010烟草BY-2细胞和蚕豆叶肉细胞原生质体微丝骨架及la trunculin B处理对其分布的影响,浙江大学学报:农业与生命科学版,36(5):497-502)
Zhang D.,Du Q.,Xu B.,Zhang Z.,and Li B.,2010,The actin multigene family in Populus:organization,expression and phylogenetic analysis,Mol.Genet.Genomics,284:105-119
Zhang M.B.,and Wang X.J.,2004,Morphogenesis of plant cell Xibei Zhiwu Xuebao(Acta Botanica Boreail-Occidentalia Sinica),24(1):154-160(张妙彬和王小菁,2004,植物细胞形态构建,西北植物学报,24(1):154-160)
Zhang X.T.,2012,Molecular cloning and functional analysis o actin-binding protein gene(Gh VLN1)in cotton,Thesis fo M.S.,Nanjing Agricultural University,Supervisor:Liu Kpp.9-12(张晓彤,2012,棉花Actin结合蛋白基因(Gh VLN1的克隆与功能分析,硕士学位论文,南京农业大学,导师刘康,pp.9-12)