盐胁迫对拟南芥Profilins和ADFs转录水平的影响
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摘要
盐胁迫是影响植物生长发育的重要影响因子,是造成农业减产的重要因素之一。因此抗盐基因的功能研究具有重要的理论意义和应用价值。以往的研究表明,肌动蛋白细胞骨架在植物对盐胁迫的响应中起着重要作用,但肌动蛋白结合蛋白在盐胁迫中的作用尚不清楚。文章以拟南芥为试验材料,采用实时荧光定量聚合酶链式反应(polymerase chain reaction,PCR)技术探讨profilins和ADFS基因在盐胁迫处理下的表达。结果表明,用150mmol/L NaCl处理2周野生型拟南芥幼苗时profilins和ADFs基因的表达量明显升高,说明profilins和ADFs基因可能受到NaCl诱导。该实验结果为进一步研究该基因调控植物抗盐的机制提供了基础。
Salt stress is an important factor which affects the growth and development of plants,and harms the agricultural production.Therefore,the research on the function of salt-resistant genes has important theoretical significance and application value.Previous studies have shown that the actin cytoskeleton plays an important role in plant responses to salt stress,but the role of actin binding protein in salt stress is unclear.In this paper,the expressions of profilins and ADFs genes under salt stress were investigated by real-time fluorescence quantitative polymerase chain reaction(PCR)using Arabidopsis thaliana as the experimental material.The results showed that the expressions of profilins and ADFs significantly increased in wild type Arabidopsis thaliana seedlings of two weeks under150 mmol/L NaCl.The results indicated that profilins and ADFs were induced by salt.The results provide a basis for further study on the mechanism of the gene regulating plant responses to salt stress.
Salt stress is an important factor which affects the growth and development of plants,and harms the agricultural production.Therefore,the research on the function of salt-resistant genes has important theoretical significance and application value.Previous studies have shown that the actin cytoskeleton plays an important role in plant responses to salt stress,but the role of actin binding protein in salt stress is unclear.In this paper,the expressions of profilins and ADFs genes under salt stress were investigated by real-time fluorescence quantitative polymerase chain reaction(PCR)using Arabidopsis thaliana as the experimental material.The results showed that the expressions of profilins and ADFs significantly increased in wild type Arabidopsis thaliana seedlings of two weeks under150 mmol/L NaCl.The results indicated that profilins and ADFs were induced by salt.The results provide a basis for further study on the mechanism of the gene regulating plant responses to salt stress.
引文
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[11] RUZICKA D R,KANDASAMY M K,MCKINNEY E C,et al.The ancient subclasses of Arabidopsisactin depolymerizing factor genesexhibit novel anddifferential expression[J].The Plant Journal,2007,52:460-472.
[12]王楠,吕香玲,李亮,等.植物肌动蛋白解聚因子ADF的研究进展[J].西北植物学报,2015,35(11):2349-2354.
[13]陈琼,黄善金,于荣.植物微丝骨架动态变化的调节[J].植物生理学报,2011,47(1):18-26.
[14] BURGOS RIVERA B,RUZICKA D R,DEAL R B,et al.Actin depolymerizing factors controls development and gene expression in Arabidopsis[J].Plant Molecular Biology,2008,68:619-632.
[15]徐阳,原丽平,陈金玲,等.肌动蛋白解聚因子/cofilin功能的研究进展[J].生命科学,2012,24(1):13-16.
[2] MAKIOKA A,KUNAQAI M,KOBAYASHI S,et al.Entamoeba invadens identification of ADF/cofilin and their expression analysis in relation to encystation and excystation[J].Experimental Parasitology,2011,127(1):195-201.
[3] FENG Y,LIU Q B,XUE Q Z.Comparative study of rice and Arabidopsis Actin-depolymerizing factors gene families[J].Plant Physiology,2006,163(1):69-79.
[4]翟中和,王喜忠,丁明孝.细胞生物学[M].3版.北京:高等教育出版社,2007:194-206.
[5] ROLAND J,BERRO J,MICHELOT A,et al.Stochastic severing ofactin filaments by actin depolymerizing factor/cofilin controls the emergence of a steady dynamical regime[J].Biophys Journal,2008,94(6):2082-2094.
[6] NAKANO K,MABUCHI I.Actin-depolymerizing protein Adf1is required for formation and maintenance of the contractile ring during cytokinesis in fission yeast[J].Molecular Biology of the Cell,2006,17(4):1933-1945.
[7] RUHLANDT G,LANGE U,GROLIG F.Profilins purified from higher plants bind to actin from cardiac muscle and to actin from a green alga[J].Plant and Cell Physiology,1994,35(5):849-854.
[8] KUURE S,CEBRIAN C,QUENTIN M,et al.P87Actin depolymerizing factors Cfl and Dstnare required for ureteric bud branching morphogenesis[J].Plos Geneicst,2010,6(10):76-79.
[9] KANDASAMY M K,MCKINNEY E C,MEAGHER R B.Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues[J].Cell Motility and the Cytoskeleton,2002,52:22-32.
[10]WITTENMAYER N,ROTHKEGEL M,JOCKUSCH B M,et al.Functional characterization of gerrn fiuorescent protein peofilin fusion proteins[J].European Journal of Biochemistry,2000,267(16):5247-5256.
[11] RUZICKA D R,KANDASAMY M K,MCKINNEY E C,et al.The ancient subclasses of Arabidopsisactin depolymerizing factor genesexhibit novel anddifferential expression[J].The Plant Journal,2007,52:460-472.
[12]王楠,吕香玲,李亮,等.植物肌动蛋白解聚因子ADF的研究进展[J].西北植物学报,2015,35(11):2349-2354.
[13]陈琼,黄善金,于荣.植物微丝骨架动态变化的调节[J].植物生理学报,2011,47(1):18-26.
[14] BURGOS RIVERA B,RUZICKA D R,DEAL R B,et al.Actin depolymerizing factors controls development and gene expression in Arabidopsis[J].Plant Molecular Biology,2008,68:619-632.
[15]徐阳,原丽平,陈金玲,等.肌动蛋白解聚因子/cofilin功能的研究进展[J].生命科学,2012,24(1):13-16.