肠道可培养共生菌对橘小实蝇不育雄虫生物学特征的影响
|
摘要
橘小实蝇是一种对果、蔬产业造成严重经济损失的害虫,应用昆虫不育技术(SIT)防治橘小实蝇是一种切实可行的科学手段,但辐照会使不育雄虫受到一定程度的损伤。本研究通过在饲料中添加抗生素对肠道灭菌处理来探究肠道可培养共生菌对橘小实蝇生物学及形态学参数的影响。结果表明,抗生素处理组在不育雄虫的蛹重、逆境存活率以及竞争交配力等均显著低于对照组。另外,抗生素处理组和对照组不育雄虫的存活率曲线图表明处理组不育雄虫的寿命时长显著低于对照组,利用Weibull分布拟合模型判断得知两组橘小实蝇存活率曲线均符合基本模型中的Ⅰ型。本研究为进一步利用肠道可培养共生菌来改善橘小实蝇不育雄虫的生理健康提供理论基础。
Bactrocera dorsalis(Hendel) is a notorious agricultural pest of fruit and vegetable industry. Sterile insect technique is widely used in control of B. dorsalis, but sterile males may be injured during radiation. In this study, the influence of cultivable gut microbes on biology and morphology of B. dorsalis was investigated through adding two antibiotics to the larval diet. The pupal weight, survival rate and mating competition of the antibioticstreated sterile males were significantly lower than the control sterile males. Further, although the survival curves of the antibiotics-treated and control sterile males fitted with pattern I of the basic Weibull distribution model, longevity of the antibiotics-treated sterile males was significantly shorter than that of the control. This result suggest that gut microbes can be used to reduce the irradiation-induced damage to Bactrocera dorsalis.
Bactrocera dorsalis(Hendel) is a notorious agricultural pest of fruit and vegetable industry. Sterile insect technique is widely used in control of B. dorsalis, but sterile males may be injured during radiation. In this study, the influence of cultivable gut microbes on biology and morphology of B. dorsalis was investigated through adding two antibiotics to the larval diet. The pupal weight, survival rate and mating competition of the antibioticstreated sterile males were significantly lower than the control sterile males. Further, although the survival curves of the antibiotics-treated and control sterile males fitted with pattern I of the basic Weibull distribution model, longevity of the antibiotics-treated sterile males was significantly shorter than that of the control. This result suggest that gut microbes can be used to reduce the irradiation-induced damage to Bactrocera dorsalis.
引文
[1]袁瑞玲,杨珊,冯丹,等.温度、湿度、光照对桔小实蝇飞行能力的影响[J].环境昆虫学报,2016,38(5):903-911.
[2]Ji Q,Bi K,Chen J.Response of egg-pupal parasitoid Fopius arisanus(Sonan)to infochemicals from the host eggs’surface of Bactrocera dorsalis(Hendel)[J].Journal of Asia-Pacific Entomology,2016,19(4):1151-1157.
[3]王晓梅,孙静双,李志强,等.北京地区桔小实蝇的发生规律及防治措施研究[J].中国南方果树,2016,45(3):27-30.
[4]王玉玲.桔小实蝇的发生与诱杀防治研究进展[J].环境昆虫学报,2013,35(2):253-259.
[5]Silva N,Dantas L,Calisto R,et al.Improving an adult holding system for Mediterranean fruit fly,Ceratitis capitata,to enhance sterile male performance[J].Journal of Applied Entomology,2013,137(s1):230-237.
[6]Sookar P,Bhagwant S,Khayrattee F B,et al.Mating compatibility of wild and sterile melon flies,Bactrocera cucurbitae(Diptera:Tephritidae)treated with entomopathogenic fungi[J].Journal of Applied Entomology,2014,138(6):409-417.
[7]Dominiak B C,Sundaralingam S,Jiang L,et al.Evaluating irradiation dose for sterility induction and quality control of mass-produced fruit fly Bactrocera tryoni(Diptera:Tephritidae)[J].Journal of Economic Entomology,2014,107(3):1172-1178.
[8]Zahan A,Latif M A,Hossain M A,et al.Optimization of sterile male ratio of oriental fruit fly,Bactrocera dorsalis(Hendel)[J].Saarc Journal of Agriculture,2016,13(2):50-60.
[9]郭军,吴杰,邓先余,等.昆虫肠道菌群的功能研究进展[J].应用昆虫学报,2015(6):1345-1352.
[10]Castagnola A,Jurat-Fuentes J L.Intestinal regeneration as an insect resistance mechanism to entomopathogenic bacteria[J].Current Opinion in Insect Science,2016,15:104-110.
[11]鲁迎新,刘彦群,李群,等.昆虫肠道微生物多样性研究进展[J].河南农业科学,2016,45(11):1-7.
[12]姚明燕,季清娥,陈家骅.实蝇肠杆菌科共生菌的研究进展[J].生物安全学报,2017,26(2):103-110.
[13]洪静芳,季清娥,陈家骅.卵期辐照对橘小实蝇生长发育繁殖的影响[J].核农学报,2014,28(3):418-422.
[14]Lux S A,Vilardi J C,Liedo P,et al.Effects of irradiation on the courtship behavior of medfly(Diptera,Tephritidae)mass reared for the sterile insect technique[J].Florida Entomologist,2002,85(1):102-112.
[15]邹寿发,章玉苹,陈明洋,等.喜树碱对桔小实蝇成虫影响的研究[J].广东农业科学,2010,37(2):89-92.
[16]Moran N A.Symbiosis as an adaptive process and source of phenotypic complexity[J].Proceedings of the National Academy of Sciences,2007,104:8627-8633.
[17]柳丽君,李志红,戴阳.实蝇共生菌研究进展[J].生物安全学报,2011,20(2):91-99.
[18]Kounatidis I,Crotti E,Sapountzis P,et al.Acetobacter tropicalis is a major symbiont of the olive fruit fly(Bactrocera oleae).[J].Applied and Environmental Microbiology,2009,75(10):3281-3288.
[19]Wang H,Jin L,Peng T,et al.Identification of cultivable bacteria in the intestinal tract of Bactrocera dorsalis from three different populations and determination of their attractive potential[J].Pest Management Science,2014,70(1):80-87.
[20]Behar A,Jurkevitch E,Yuval B.Bringing back the fruit into fruit fly-bacteria interactions[J].Molecular Ecology,2008,17(5):1375-1386.
[21]Christopherw W,John P,Phillipw T.Activity patterns of Queensland fruit flies(Bactrocera tryoni)are affected by both mass-rearing and sterilization[J].Physiological Entomology,2010,35(2):148-153.
[22]HossainM D,Wadud M A,Khan S A,et al.Sensitivity of oriental fruitfly,Bactrocera dorsalis(Hendel)(Diptera:Tephritidae)to gamma radiation[J].Nuclear Science and Applications,2006,15:108-112.
[23]Lauzon C R,Potter S E.Description of the irradiated and nonirradiated midgut of Ceratitis capitata Wiedemann(Diptera:Tephritidae)and Anastrepha ludens Loew(Diptera:Tephritidae)used for sterile insect technique[J].Journal of Pest Science,2012,85(2):217-226.
[24]王延南,朱麟.肠道微生物对瓜实蝇营养利用及其生长的影响[J].基因组学与应用生物学,2017(6):2405-2409.
[25]王四宝,曲爽.昆虫共生菌及其在病虫害防控中的应用前景[J].中国科学院院刊,2017,32(8):863-872.
[26]张振宇,圣平,黄胜威,等.昆虫肠道微生物的多样性、功能及应用[J].生物资源,2017,39(4):231-239.
[27]陈勃生,鲁兴萌,邵勇奇.鳞翅目昆虫肠道微生物的多样性及其与宿主的相互作用[J].昆虫学报,2017,60(6):710-722.
[28]Gavriel S,Jurkevitch E,Gazit Y,et al.Bacterially enriched diet improves sexual performance of sterile male Mediterranean fruit flies[J].Journal of Applied Entomology,2011,135(7):564-573.
[29]Ami E B,Yuval B,Jurkevitch E.Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata(Diptera:Tephritidae)improves sterile male sexual performance[J].Isme Journal,2010,4(1):28-37.
[30]万伦.柑橘大实蝇肠道细菌分子多态性分析及其生理作用研究[D].武汉:华中农业大学,2013.
[31]王洪秀.橘小实蝇(Bactrocera dorsalis)成虫肠道细菌分子多态性分析及其功能研究[D].武汉:华中农业大学,2011.
[2]Ji Q,Bi K,Chen J.Response of egg-pupal parasitoid Fopius arisanus(Sonan)to infochemicals from the host eggs’surface of Bactrocera dorsalis(Hendel)[J].Journal of Asia-Pacific Entomology,2016,19(4):1151-1157.
[3]王晓梅,孙静双,李志强,等.北京地区桔小实蝇的发生规律及防治措施研究[J].中国南方果树,2016,45(3):27-30.
[4]王玉玲.桔小实蝇的发生与诱杀防治研究进展[J].环境昆虫学报,2013,35(2):253-259.
[5]Silva N,Dantas L,Calisto R,et al.Improving an adult holding system for Mediterranean fruit fly,Ceratitis capitata,to enhance sterile male performance[J].Journal of Applied Entomology,2013,137(s1):230-237.
[6]Sookar P,Bhagwant S,Khayrattee F B,et al.Mating compatibility of wild and sterile melon flies,Bactrocera cucurbitae(Diptera:Tephritidae)treated with entomopathogenic fungi[J].Journal of Applied Entomology,2014,138(6):409-417.
[7]Dominiak B C,Sundaralingam S,Jiang L,et al.Evaluating irradiation dose for sterility induction and quality control of mass-produced fruit fly Bactrocera tryoni(Diptera:Tephritidae)[J].Journal of Economic Entomology,2014,107(3):1172-1178.
[8]Zahan A,Latif M A,Hossain M A,et al.Optimization of sterile male ratio of oriental fruit fly,Bactrocera dorsalis(Hendel)[J].Saarc Journal of Agriculture,2016,13(2):50-60.
[9]郭军,吴杰,邓先余,等.昆虫肠道菌群的功能研究进展[J].应用昆虫学报,2015(6):1345-1352.
[10]Castagnola A,Jurat-Fuentes J L.Intestinal regeneration as an insect resistance mechanism to entomopathogenic bacteria[J].Current Opinion in Insect Science,2016,15:104-110.
[11]鲁迎新,刘彦群,李群,等.昆虫肠道微生物多样性研究进展[J].河南农业科学,2016,45(11):1-7.
[12]姚明燕,季清娥,陈家骅.实蝇肠杆菌科共生菌的研究进展[J].生物安全学报,2017,26(2):103-110.
[13]洪静芳,季清娥,陈家骅.卵期辐照对橘小实蝇生长发育繁殖的影响[J].核农学报,2014,28(3):418-422.
[14]Lux S A,Vilardi J C,Liedo P,et al.Effects of irradiation on the courtship behavior of medfly(Diptera,Tephritidae)mass reared for the sterile insect technique[J].Florida Entomologist,2002,85(1):102-112.
[15]邹寿发,章玉苹,陈明洋,等.喜树碱对桔小实蝇成虫影响的研究[J].广东农业科学,2010,37(2):89-92.
[16]Moran N A.Symbiosis as an adaptive process and source of phenotypic complexity[J].Proceedings of the National Academy of Sciences,2007,104:8627-8633.
[17]柳丽君,李志红,戴阳.实蝇共生菌研究进展[J].生物安全学报,2011,20(2):91-99.
[18]Kounatidis I,Crotti E,Sapountzis P,et al.Acetobacter tropicalis is a major symbiont of the olive fruit fly(Bactrocera oleae).[J].Applied and Environmental Microbiology,2009,75(10):3281-3288.
[19]Wang H,Jin L,Peng T,et al.Identification of cultivable bacteria in the intestinal tract of Bactrocera dorsalis from three different populations and determination of their attractive potential[J].Pest Management Science,2014,70(1):80-87.
[20]Behar A,Jurkevitch E,Yuval B.Bringing back the fruit into fruit fly-bacteria interactions[J].Molecular Ecology,2008,17(5):1375-1386.
[21]Christopherw W,John P,Phillipw T.Activity patterns of Queensland fruit flies(Bactrocera tryoni)are affected by both mass-rearing and sterilization[J].Physiological Entomology,2010,35(2):148-153.
[22]HossainM D,Wadud M A,Khan S A,et al.Sensitivity of oriental fruitfly,Bactrocera dorsalis(Hendel)(Diptera:Tephritidae)to gamma radiation[J].Nuclear Science and Applications,2006,15:108-112.
[23]Lauzon C R,Potter S E.Description of the irradiated and nonirradiated midgut of Ceratitis capitata Wiedemann(Diptera:Tephritidae)and Anastrepha ludens Loew(Diptera:Tephritidae)used for sterile insect technique[J].Journal of Pest Science,2012,85(2):217-226.
[24]王延南,朱麟.肠道微生物对瓜实蝇营养利用及其生长的影响[J].基因组学与应用生物学,2017(6):2405-2409.
[25]王四宝,曲爽.昆虫共生菌及其在病虫害防控中的应用前景[J].中国科学院院刊,2017,32(8):863-872.
[26]张振宇,圣平,黄胜威,等.昆虫肠道微生物的多样性、功能及应用[J].生物资源,2017,39(4):231-239.
[27]陈勃生,鲁兴萌,邵勇奇.鳞翅目昆虫肠道微生物的多样性及其与宿主的相互作用[J].昆虫学报,2017,60(6):710-722.
[28]Gavriel S,Jurkevitch E,Gazit Y,et al.Bacterially enriched diet improves sexual performance of sterile male Mediterranean fruit flies[J].Journal of Applied Entomology,2011,135(7):564-573.
[29]Ami E B,Yuval B,Jurkevitch E.Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata(Diptera:Tephritidae)improves sterile male sexual performance[J].Isme Journal,2010,4(1):28-37.
[30]万伦.柑橘大实蝇肠道细菌分子多态性分析及其生理作用研究[D].武汉:华中农业大学,2013.
[31]王洪秀.橘小实蝇(Bactrocera dorsalis)成虫肠道细菌分子多态性分析及其功能研究[D].武汉:华中农业大学,2011.