水凝胶对大鼠Ⅱ度放射性皮炎创面愈合影响机制探讨
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摘要
目的放射性皮炎是肿瘤患者治疗中常见的一种反应之一,严重影响到肿瘤患者治疗进程。本研究探讨Urgo水凝胶治疗Wistar大鼠Ⅱ度放射性皮炎愈合过程中创面愈合率及其机制,为临床治疗放射性皮炎提供新方法。方法建立Wistar大鼠Ⅱ度放射性皮炎模型,随机分成3组,实验组给予Ugro水凝胶0.2mm涂层,比亚芬组(传统组)给予比亚芬0.2mm涂层,生理盐水组(对照组)给予0.9%生理盐水喷雾创面处理。通过HE染色、免疫组化及图像分析,测定各组Wistar大鼠放疗后7、14、21和28d创面肉芽组织、创面面积及创面中碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)和转化生长因子-β1(transforming growth factor-β1,TGF-β1)表达。结果 X射线放射后大鼠出现脱毛或皮肤溃疡现象,实验组创面面积在第21d为(0.900±0.070)cm2,低对照组(2.317±0.280)cm2和传统组(1.550±0.080)cm2,差异有统计学意义,F=20.4,P<0.05;实验组创面面积在第28d为(1.061±0.054)cm2,明显低于对照组(2.520±0.140)cm2和传统组(1.453±0.080)cm2,差异有统计学意义,F=68.84,P<0.05。实验组第28dbFGF阳性表达量为55.00±1.00,高于传统组43.60±1.81,差异有统计学意义,t=5.52,P=0.000 6。实验组第28天TGF-β1阳性表达量为51.00±3.69,高于传统组36.40±4.41,差异有统计学意义,t=2.54,P=0.035。结论 Ugro水凝胶能加快Wistar大鼠Ⅱ度放射性皮炎创面的愈合,减轻创面的炎症反应,促进弹力纤维和胶原的修复,其机制与表皮生长因子的表达有关。
OBJECTIVE To investigate the effect of Urgo Hydrogel on theⅡ degree radiation dermatitis in Wistar rat and its influence on the histomorphological and collagenous change of wound.METHODS Thirty-six Wistar female rats withⅡdegree radiation dermatitis induced by X-RAD 225 X-ray 40 Gy one time radiation were randomly divided into3 groups and treated with Urgo Hydrogel(Experimental group),Trolamine Cream(Control group),and normal saline respectively(Normal group).The area of ulcer of all groups were measured,stained by HE staining and IHC staining for observing the changes in histomorphology and levels of bFGF bFGF,TGF-β1 in the wound tissue.RESULTS X-irradiation induced epilation or skin ulcers in almost all rats.The wound area in Urgo Hydrogel group(0.900±0.07)cm2 was lower significantly than that in the normal group(2.317±0.280)cm2 and control group(1.550±0.080)cm2 at 21 st day(F=20.4,P<0.05).The wound area in Urgo Hydrogel group(1.061±0.0540)cm2 was lower significantly than that in the normal group(2.520±0.140)cm2 and control group(1.453±0.080)cm2 at the 28 th day(F=68.84,P<0.05).The total expression level of bFGF in experimental group was 55.00±1.00,which was significantly higher than that in control group(43.60±1.81)at the 28 th day(t=5.52,P<0.001).The total expression level of TGF-β1 in experimental group was 51.00±3.69,which was significantly higher than that in control group(36.40±4.41)at 28 th day(t=2.54,P=0.035).CONCLUSION The Urgo Hydrogel can promote the ulcer healing ofⅡ degree radiation dermatitis,reduce the inflammatory reaction in wound,and promote the repair of elastic fibers and collagen related with the expressions of epidermal growth factors.
OBJECTIVE To investigate the effect of Urgo Hydrogel on theⅡ degree radiation dermatitis in Wistar rat and its influence on the histomorphological and collagenous change of wound.METHODS Thirty-six Wistar female rats withⅡdegree radiation dermatitis induced by X-RAD 225 X-ray 40 Gy one time radiation were randomly divided into3 groups and treated with Urgo Hydrogel(Experimental group),Trolamine Cream(Control group),and normal saline respectively(Normal group).The area of ulcer of all groups were measured,stained by HE staining and IHC staining for observing the changes in histomorphology and levels of bFGF bFGF,TGF-β1 in the wound tissue.RESULTS X-irradiation induced epilation or skin ulcers in almost all rats.The wound area in Urgo Hydrogel group(0.900±0.07)cm2 was lower significantly than that in the normal group(2.317±0.280)cm2 and control group(1.550±0.080)cm2 at 21 st day(F=20.4,P<0.05).The wound area in Urgo Hydrogel group(1.061±0.0540)cm2 was lower significantly than that in the normal group(2.520±0.140)cm2 and control group(1.453±0.080)cm2 at the 28 th day(F=68.84,P<0.05).The total expression level of bFGF in experimental group was 55.00±1.00,which was significantly higher than that in control group(43.60±1.81)at the 28 th day(t=5.52,P<0.001).The total expression level of TGF-β1 in experimental group was 51.00±3.69,which was significantly higher than that in control group(36.40±4.41)at 28 th day(t=2.54,P=0.035).CONCLUSION The Urgo Hydrogel can promote the ulcer healing ofⅡ degree radiation dermatitis,reduce the inflammatory reaction in wound,and promote the repair of elastic fibers and collagen related with the expressions of epidermal growth factors.
引文
[1]欧玉兰,许玉春.复方芩柏颗粒对大鼠放射性皮炎创面的影响[J].中医杂志,2010,51(3):265-267.
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[8]卢金利,刘小平,杨芳,等.芦荟凝胶抑制大鼠Ⅱ度放射性皮炎发生的机理研究[J].现代肿瘤医学,2006,14(8):930-932.
[9]戚霞露,吴亮,张陆勇.放射性皮炎的发生机制及药物防治现状[J].药学研究,2017,36(7):417-419.
[10]吴宏,李燕.奥克喷联合喜疗妥预防放射性皮肤损伤[J].实用临床医药杂志,2011,15(16):13-14.
[11]刘小平,卢金利,吴素歌,等.芦荟凝胶对大鼠Ⅱ度放射性皮炎创面愈合及表皮生长因子、碱性成纤维细胞生长因子表达的影响[J].西安交通大学学报(医学版),2006,27(1):66-68.
[12] Yu D,Li S,Wang S,et al.Development and Characterization of VEGF165-Chitosan Nanoparticles for the Treatment of Radiation-Induced Skin Injury in Rats[J].Mar Drugs,2016,14(10):182.
[13] Wang W,Luo J,Sheng W,et al.Proteomic profiling of radiationinduced skin fibrosis in rats:targeting the ubiquitin-proteasome system[J].Int J Radiat Oncol,2016,95(2):751-760.
[14]戚晓霞,蔡蕴敏.片状水凝胶敷料预防急性放射性皮炎的效果观察[J].中国临床医学,2014,21(6):728-729.
[2]王俐.金黄散联用维生素E治疗放射性皮炎与比亚芬的非劣性对照研究[D].南京:南京中医药大学,2015.
[3] Takikawa M,Sumi Y,Tanaka Y,et al.Protective effect of prostaglandin E1on radiation-induced proliferative inhibition and apoptosis in keratinocytes and healing of radiation-induced skin injury in rats[J].J Radiat Res,2012,53(3):385-394.
[4] Kumar SS,Basu M,Agrawal P,et al.Evaluation of gamma radiation-induced biochemical changes in skin for dose assesment:a study on small experimental animals[J].Disaster Med Public Health Prep,2018,24:1-6.
[5]薛俐,冯志明,易银沙,等.复方茶多酚软膏对放射性皮炎大鼠伤口愈合和表皮生长因子表达的影响[J].中国组织工程研究,2010,14(37):6891-6894.
[6] Zu G,Dou Y,Tian Q,et al.Role and mechanism of radiological protection cream in treating radiation dermatitis in rats[J].J Tradit Chin Med,2014 34(3):329-337.
[7]杨霖,于明薇,王笑民,等.黑绛丹治疗60 Co射线照射致大鼠放射性皮炎的疗效评价[J].中医杂志,2011,52(23):2040-2042.
[8]卢金利,刘小平,杨芳,等.芦荟凝胶抑制大鼠Ⅱ度放射性皮炎发生的机理研究[J].现代肿瘤医学,2006,14(8):930-932.
[9]戚霞露,吴亮,张陆勇.放射性皮炎的发生机制及药物防治现状[J].药学研究,2017,36(7):417-419.
[10]吴宏,李燕.奥克喷联合喜疗妥预防放射性皮肤损伤[J].实用临床医药杂志,2011,15(16):13-14.
[11]刘小平,卢金利,吴素歌,等.芦荟凝胶对大鼠Ⅱ度放射性皮炎创面愈合及表皮生长因子、碱性成纤维细胞生长因子表达的影响[J].西安交通大学学报(医学版),2006,27(1):66-68.
[12] Yu D,Li S,Wang S,et al.Development and Characterization of VEGF165-Chitosan Nanoparticles for the Treatment of Radiation-Induced Skin Injury in Rats[J].Mar Drugs,2016,14(10):182.
[13] Wang W,Luo J,Sheng W,et al.Proteomic profiling of radiationinduced skin fibrosis in rats:targeting the ubiquitin-proteasome system[J].Int J Radiat Oncol,2016,95(2):751-760.
[14]戚晓霞,蔡蕴敏.片状水凝胶敷料预防急性放射性皮炎的效果观察[J].中国临床医学,2014,21(6):728-729.