益气活血方对急性胃溃疡大鼠模型黏膜愈合质量研究
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摘要
目的研究分析益气活血方对急性胃溃疡大鼠模型黏膜愈合质量的影响。方法 120只成年大鼠随机分为正常组、模型组、益气活血方组3组,每组40只。冰醋酸灼烧大鼠胃窦前壁浆膜层制备胃溃疡模型。正常组、模型组、益气活血方组分别每日灌胃饲以纯净水及益气活血方水煎液,连续14 d。治疗结束后,观察溃疡胃组织黏膜损伤情况,统计溃疡发生率、溃疡指数和溃疡抑制率以及VEGF、bFGF表达水平及再生黏膜愈合质量。结果与正常组比较,模型组、益气活血方组溃疡发生率、溃疡指数均明显增加(均P<0.05),尤其模型组更加显著。益气活血方组与模型组比较,溃疡发生率和溃疡指数明显降低,溃疡抑制率显著提高(均P<0.05)。与正常组比较,模型组再生黏膜厚度、微血管数明显不足,而囊状扩张腺体数增加较明显(均P<0.05)。益气活血方组再生黏膜厚度、囊状扩张腺体数改善均优于模型组(均P<0.05)。模型组血清VEGF、bFGF的含量较正常组含量明显增加(均P<0.05)。益气活血方组血清VEGF、bFGF增加优于模型组(均P<0.05)。结论益气活血方能降低溃疡发生率和溃疡指数,提高溃疡抑制率,促进急性胃溃疡的黏膜再生,减轻炎症反应,促进VEGF、bFGF表达,从而提高溃疡愈合质量。
Objective: To discuss the influence of Yiqi Huoxue Decoction on the healing quality of gastric mucosa in rats,with acute gastric ulcer. Methods: 120 rats were randomly divided into three groups: the normal group,the model group and Yiqi Huoxue Decoction group,40 cases in each group. The model was made by glacial acetic acid which was injected to burning the placenta percreta in antral gastritis. The three groups were feed with purified water and decoction by gavage for 14 days. After treatment,the following items were recorded: the degree of gastric mucosa injury,the incidence rate of ulcer,the index of ulcer,the suppression ratio of ulcer,the expression level of VEGF and bFGF,and the healing quality of mucosa. Results: Compared with the normal group,the the incidence rate and the index of ulcer of the model group and the Yiqi Huoxue Decoction group increased obviously(P < 0.05),especially the model group. Compared with the model group,the incidence rate and index of ulcer of the Yiqi Huoxue Decoction group reduced obviously and the inhibition rate of ulcer increased obviously(P < 0.05). Compared with the normal group,the thickness of the regenerated mucosa and the amount of micrangium of the model group were obviously insufficient,and there was an obvious increase in the number of cystically dilated glands(P < 0.05). As for the Yiqi Huoxue Decoction group,there was a better improvement in the thickness of the regenerated mucosa and the number of cystically dilated glands than that of the model group(P <0.05). The expression level of VEGF and bFGF in the model group has a more obvious increase than that of the normal group(P < 0.05),and the expression level of VEGF and bFGF in the Yiqi Huoxue Decoction group has a more obvious increase than that of the model group(P < 0.05). Conclusion: Yiqi Huoxue Decoction can reduce the incidence rate and the index of ulcer,improve the inhibition of ulter,promote the mucosal regeneration of acute gastric ulcer,reduce inflammatory response and improve the expression of VEGF and bFGF to increase the healing quality of mucosa.
Objective: To discuss the influence of Yiqi Huoxue Decoction on the healing quality of gastric mucosa in rats,with acute gastric ulcer. Methods: 120 rats were randomly divided into three groups: the normal group,the model group and Yiqi Huoxue Decoction group,40 cases in each group. The model was made by glacial acetic acid which was injected to burning the placenta percreta in antral gastritis. The three groups were feed with purified water and decoction by gavage for 14 days. After treatment,the following items were recorded: the degree of gastric mucosa injury,the incidence rate of ulcer,the index of ulcer,the suppression ratio of ulcer,the expression level of VEGF and bFGF,and the healing quality of mucosa. Results: Compared with the normal group,the the incidence rate and the index of ulcer of the model group and the Yiqi Huoxue Decoction group increased obviously(P < 0.05),especially the model group. Compared with the model group,the incidence rate and index of ulcer of the Yiqi Huoxue Decoction group reduced obviously and the inhibition rate of ulcer increased obviously(P < 0.05). Compared with the normal group,the thickness of the regenerated mucosa and the amount of micrangium of the model group were obviously insufficient,and there was an obvious increase in the number of cystically dilated glands(P < 0.05). As for the Yiqi Huoxue Decoction group,there was a better improvement in the thickness of the regenerated mucosa and the number of cystically dilated glands than that of the model group(P <0.05). The expression level of VEGF and bFGF in the model group has a more obvious increase than that of the normal group(P < 0.05),and the expression level of VEGF and bFGF in the Yiqi Huoxue Decoction group has a more obvious increase than that of the model group(P < 0.05). Conclusion: Yiqi Huoxue Decoction can reduce the incidence rate and the index of ulcer,improve the inhibition of ulter,promote the mucosal regeneration of acute gastric ulcer,reduce inflammatory response and improve the expression of VEGF and bFGF to increase the healing quality of mucosa.
引文
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[12]李海涛,智绪亭.VEGF、b FGF表达和微血管密度检测在难治性胃溃疡中的意义[J].中国全科医学,2005,8(16):1311-1315.
[13]张卫平,葛惠男.益气活血方对消化性溃疡新生血管影响的初步研究[J].北京中医药,2008,28(4):256-258.
[14]殷文银,尹玉平.中药分期联合西药治疗消化性溃疡98例[J].中国中医急症,2012,21(4):664-665.
[15]朱瑾波,李玉鼎,李玉书.黄芪治疗慢性皮肤溃疡对血管生成过程的机制探讨[J].河北中医,1996,18(4):21.
[16]张建国,李强,张兆祥,等.中药对小鼠骨折后血管重建与骨折愈合方式关系的影响[J].中国骨伤,2003,16(2):84.
[17]郭金伟,葛惠男.益气活血方对消化性溃疡复发的影响[D].南京:南京中医药大学,2009.
[2]夏梅青.田素荣.内镜下治疗急性胃溃疡出血临床分析[J].现代诊断与治疗,2012,23(6):812-813.
[3]谢田.胃溃疡实验动物模型制作方法的研究进展[J].中国伤残医学,2014,22(10):309-310
[4]江国荣,张露蓉,葛惠男.益气活血方联用奥美拉唑对大鼠消化性溃疡愈合质量的影响[J].东南大学学报:医学版,2013,32(3):320-323.
[5]梁桂宁,黄媛恒,贾晓栋,等.紫菜多糖对急性酒精灌胃小鼠胃组织SOD、GSH-Px的影响[J].中国实用医药,2012,7(4):248-249.
[6]梅武轩,曾常春.乳香提取物对大鼠乙酸胃溃疡愈合质量的影响[J].中国中西医结合消化杂志,2004,12(1):34-36.
[7]姒健敏,钱云,吴加国.云母对实验性胃溃疡愈合质量的影响[J].中国中药杂志,2005,30(19):1536-1541.
[8]江国荣.张露蓉.葛惠男.益气活血方对大鼠胃溃疡愈合部位新血管的生成及促血管生成因子表达的作用[J].吉林中医药,2013,8(8):827-828.
[9]中华消化杂志编委会.消化性溃疡诊断与治疗规范(2013,深圳)[J].中华消化杂志,2014,34(2):73-76.
[10]Annewski A,Stachura J,Krause WJ.Qualityofgastriculcerhealing:anew,emergingconcept[J].Clin Gastroenterol,1991,13(Suppl1):42.
[11]永港,舒晴,邱猛进.胃溃疡患者胃黏膜不同生长因子表达的观察[J].中国基层医药,2006,13(5):829-830.
[12]李海涛,智绪亭.VEGF、b FGF表达和微血管密度检测在难治性胃溃疡中的意义[J].中国全科医学,2005,8(16):1311-1315.
[13]张卫平,葛惠男.益气活血方对消化性溃疡新生血管影响的初步研究[J].北京中医药,2008,28(4):256-258.
[14]殷文银,尹玉平.中药分期联合西药治疗消化性溃疡98例[J].中国中医急症,2012,21(4):664-665.
[15]朱瑾波,李玉鼎,李玉书.黄芪治疗慢性皮肤溃疡对血管生成过程的机制探讨[J].河北中医,1996,18(4):21.
[16]张建国,李强,张兆祥,等.中药对小鼠骨折后血管重建与骨折愈合方式关系的影响[J].中国骨伤,2003,16(2):84.
[17]郭金伟,葛惠男.益气活血方对消化性溃疡复发的影响[D].南京:南京中医药大学,2009.