海桐种子醇提物中挥发性成分的GC-MS分析及抗内毒素活性测定
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摘要
目的研究海桐种子乙醇总提取物(ethanol extract from seeds of Pittosporum tobira Ait,EESPTA)的挥发性成分及相对含量,考察其抗内毒素活性。方法以水蒸气蒸馏法提取EESPTA的挥发性成分,用气-质联用技术初步分析鉴定,并用峰面积归一化法测定各成分相对含量,采用终点基质显色法鲎实验检测其对细菌内毒素的清除效果,考察其抗内毒素活性。结果共检测出并初步分析鉴定32个色谱峰,占该挥发性成分总量的99.82%。在EESPTA的挥发性成分中,1R,4S,7S,11R-2,2,4,8-四甲基三环[5.3.1.0(4,11)]十一碳-8-烯含量高达54.78%,其他含量较高的成分有2-亚甲基-胆甾烷-3-醇(7.27%)、蛇麻烷-1,6-二烯-3-醇(6.23%)、4-甲基-5-癸醇(4.11%)、绿花白千层醇(3.86%)、别香橙烯(3.80%)、反式-2,3-二甲基丙烯酸(3.50%)、二氢-噻喃-3,5-二醇-四氢-4-甲基-4-氮-二乙酸乙酯(2.04%)等成分。结论 EESPTA具有较明显的抗细菌内毒素活性,并呈浓度依赖关系。为进一步开发利用海桐药用价值提供了科学依据。
OBJECTIVE To analyze the volatile components in ethanol extract from seeds of Pittosporum tobira Ait.(EESPTA) and investigate the anti-endotoxin activity of EESPTA in vitro.METHODS The volatile components in EESPTA were extracted with steam distillation method and then analysized by GC-MS.The relative content of each component was calculated by peak area normalization.The anti-endotoxin activity of EESPTA in vitro was investigated by CEPTAL(chromogenic end-point tachypleus amebocyte lysate assay).RESULTS The 32 peaks were detected and then identified preliminary,which made up 99.82% of the total volatile components.The anti-endotoxin activity of EESPTA in vitro was observed in concentration-dependent manner from CEPTAL assay.The highest content constituent was 1R,4S,7S,11R-2,2,4,8-tetramethyltricyclo [5.3.1.0(4,11)] undec-8-ene(54.78%),and other relative high content constituents were 2-methylene-cholestan-3-ol(7.27%),humulane-1,6-dien-3-ol(6.23%),4-methyl-5-decanol(4.11%),viridiflorol(3.86%),Alloaromadendrene(3.80%),2-methyl-2-butenoic acid(3.50%),et al.CONCLUSION EESPTA has obvious anti-endotoxin activity with a concentration dependence.This study provides a scientific basis for further development and utilization of Pittosporum tobira Ait..
OBJECTIVE To analyze the volatile components in ethanol extract from seeds of Pittosporum tobira Ait.(EESPTA) and investigate the anti-endotoxin activity of EESPTA in vitro.METHODS The volatile components in EESPTA were extracted with steam distillation method and then analysized by GC-MS.The relative content of each component was calculated by peak area normalization.The anti-endotoxin activity of EESPTA in vitro was investigated by CEPTAL(chromogenic end-point tachypleus amebocyte lysate assay).RESULTS The 32 peaks were detected and then identified preliminary,which made up 99.82% of the total volatile components.The anti-endotoxin activity of EESPTA in vitro was observed in concentration-dependent manner from CEPTAL assay.The highest content constituent was 1R,4S,7S,11R-2,2,4,8-tetramethyltricyclo [5.3.1.0(4,11)] undec-8-ene(54.78%),and other relative high content constituents were 2-methylene-cholestan-3-ol(7.27%),humulane-1,6-dien-3-ol(6.23%),4-methyl-5-decanol(4.11%),viridiflorol(3.86%),Alloaromadendrene(3.80%),2-methyl-2-butenoic acid(3.50%),et al.CONCLUSION EESPTA has obvious anti-endotoxin activity with a concentration dependence.This study provides a scientific basis for further development and utilization of Pittosporum tobira Ait..
引文
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[11]SONG X K,CAO Z L,GUO L,et al.GC-MS analysis of volatile components from trunk of Michelia macclurei Dandy.and the Inhibition of ETMMD on growth of MDA-MB-231cell lines and its apoptosis-inducing[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(8):911-915.
[2]XIAO B K,HUANG R Q,YANG J Y,et al.Research progress on chemical constituents and pharmacological effects of Pittosporum[J].Lishizhen Med Mater Med Res(时针国医国药),2011,22(6):1474-1475
[3]ZHANG Z Y,TURLAND N J.New Synonymies of some species of Pittosporum.(Pittosporaceae)[J].J Systematics Evolution,2002,40(2):183-186.
[4]ZHANG H,XIANG J F,TAN L,et al.Anti-tumor activity of Erythrina variegata L.extract and its mechanism of action[J].Acta Pharm Sin(药学学报),2009,44(12):1359-1363.
[5]LI L L,ZHOU W M,HONG D F,et al.Preliminary study on antimicrobial activity of the seeds and pericarp of Pittosporum tobira Ait.[J].Acta Agric Boreali-occidentalis Sin(西北农业学报),2007,16(3):274-276.
[6]LEI L M,PAN Q P.Recent progress chemistry,pharmacology,quality control extraction methods Radix Isatidis.[J].Lishizhen Med Mater Med Res(时珍国医国药),2007,18(10):2578-2580.
[7]中国药典.一部[M].2015.
[8]SUN Z,LIU H X,HUANG C S.Study on chemical constituents and bioactivity of Pittosporum Tobira.[J].Technol Dev Chem Indust,2014,43(1):22-26.
[9]WANG W,ZHANG W M.The functional characteristics of monounsaturated fatty acid[J].Food Nutr China(中国食物与营养),2005(4):44-46.
[10]SONG X K,CAO Z L,GUO L,et al.Effect and possible mechanism of the extract of barks of Michelia macclurei Dandy.on apoptosis-inducing in Hep G2 cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(7):777-780.
[11]SONG X K,CAO Z L,GUO L,et al.GC-MS analysis of volatile components from trunk of Michelia macclurei Dandy.and the Inhibition of ETMMD on growth of MDA-MB-231cell lines and its apoptosis-inducing[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(8):911-915.