桃仁在小鼠生理性月经样模型中对子宫内膜崩解的作用
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摘要
目的:研究桃仁在小鼠生理性月经模型中的作用。方法:假孕雌鼠蜕膜化诱导后切除双侧卵巢,引发孕酮撤退,造成蜕膜化子宫内膜崩解坏死,模拟月经样出血,建立小鼠生理性月经样模型。在孕酮撤退前至孕酮撤退后24h给予模型小鼠桃仁30mg/(kg·次),观察小鼠子宫大体形态与组织形态变化。结果:孕酮撤退24h后,与对照组相比,给予桃仁的小鼠子宫颜色较深,充血明显;崩解的蜕膜化组织以及崩解组织外围的基底层,有大量红细胞渗出。而空白对照组未见此现象。桃仁处理小鼠脱膜相对崩解面积与对照组未见显著差异,而其相对充血面积高于对照组(P=0.009)。结论:桃仁能够促进小鼠月经样模型崩解期的红细胞渗出,其药理作用与作用机制有待进一步研究。
Objective: To study the effect of semen persicae on mouse model with physiological menstrual-like. Methods: The pseudopregnant mouse received bilateral ovariectomy after artificial decidualization, whichtriggered progesterone withdrawal, resulted in endometrium break down, and mimiced the physiology of menstruation. Semen persicae was administrated to the mouse model in semen persicae group onlybefore progesterone withdrawal until 24 hours(h) after progesterone withdrawal, and the general morphology and histomorphometry of mousewere observed. Results:At 24 h after progesterone withdrawal,the color of mouse uterus insemen persicae group was darker, and the hyperemia was more pronounced, when compared with those of mouse in control group. There was larger number of red blood cells exudation in the break down area of endometrium, as well as the basal layer adjacent to peripheral endometrium, and the red blood cells were widely distributed among endometrial stromal cells, while the above phenomenon were not observed in the control group. Statistical analysis showed that the relative breakdown area and relative hyperemia area ofmouse in semen persicae group were similar to those of mousein control group, but the relative hyperemia area ofmouse in semen persicae group was significant higher that of mouse in control group(P=0.009). Conclusion: Semen persicaecan promote the exudation of red blood cell in mouse model withphysiologicalmenstrual-like, however its pharmacological effects and functional mechanism need to be further studied.
Objective: To study the effect of semen persicae on mouse model with physiological menstrual-like. Methods: The pseudopregnant mouse received bilateral ovariectomy after artificial decidualization, whichtriggered progesterone withdrawal, resulted in endometrium break down, and mimiced the physiology of menstruation. Semen persicae was administrated to the mouse model in semen persicae group onlybefore progesterone withdrawal until 24 hours(h) after progesterone withdrawal, and the general morphology and histomorphometry of mousewere observed. Results:At 24 h after progesterone withdrawal,the color of mouse uterus insemen persicae group was darker, and the hyperemia was more pronounced, when compared with those of mouse in control group. There was larger number of red blood cells exudation in the break down area of endometrium, as well as the basal layer adjacent to peripheral endometrium, and the red blood cells were widely distributed among endometrial stromal cells, while the above phenomenon were not observed in the control group. Statistical analysis showed that the relative breakdown area and relative hyperemia area ofmouse in semen persicae group were similar to those of mousein control group, but the relative hyperemia area ofmouse in semen persicae group was significant higher that of mouse in control group(P=0.009). Conclusion: Semen persicaecan promote the exudation of red blood cell in mouse model withphysiologicalmenstrual-like, however its pharmacological effects and functional mechanism need to be further studied.
引文
[1] Xu X B, He B, Wang J D. Menstrual-like changes in mice are provoked through the pharmacologic withdrawal of progesterone using mifepristone following induction of decidualization.[J]. Hum Reprod, 2007,22(12):3184-3191.
[2] 曹惠子, 徐祥波, 陈西华, 等. 利用假孕小鼠建立小鼠行经模型的研究[J]. 生殖医学杂志, 2010,19(1):35-39.
[3] 齐瑞文. 中医治疗妇科月经不调的临床探析[J]. 中国社区医师, 2015(14):91-92.
[4] 朱萱萱, 朱芳, 施荣山, 等. 桃仁、防己提取物对大鼠血小板聚集作用的研究[J]. 中医药研究, 2000(3):44-45.
[5] Ge Renyong, Zhou Chuhua She Yunchu..Department of Physiology, Jiangxi College of Traditional Chinese Medicine. INFLUENCES OF STIGMA CROCI AND SEMEN PERSICAE ON FUNCTION OF OVARY-UTERUS IN PSEUDOPREGNANT RATS[J].中医杂志(英文版),1983,(1):23-26.
[6] Xu X, Chen X, Li Y, et al. Cyclooxygenase-2 Regulated by the Nuclear Factor-kappaB Pathway Plays an Important Role in Endometrial Breakdown in a Female Mouse Menstrual-like Model.[J]. Endocrinology, 2013,154(8):2900-2911.
[7] 汪宁, 刘青云, 彭代银, 等. 桃仁不同提取物抗血栓作用的实验研究[J]. 中药材, 2002,(6):414-415.
[8] 耿涛, 谢梅林, 彭少平. 桃仁提取物抗大鼠心肌缺血作用的研究[J]. 苏州大学学报(医学版), 2005,(2):238-240.
[9] Liu L, Duan J A, Tang Y, et al. Taoren-Honghua herb pair and its main components promoting blood circulation through influencing on hemorheology, plasma coagulation and platelet aggregation.[J]. J Ethnopharmacol, 2012,139(2):381-387.
[10] 文川, 徐浩, 黄启福, 等. 活血中药对ApoE基因缺陷小鼠血脂及动脉粥样硬化斑块炎症反应的影响[J]. 中国中西医结合杂志, 2005,(4):345-349.
[2] 曹惠子, 徐祥波, 陈西华, 等. 利用假孕小鼠建立小鼠行经模型的研究[J]. 生殖医学杂志, 2010,19(1):35-39.
[3] 齐瑞文. 中医治疗妇科月经不调的临床探析[J]. 中国社区医师, 2015(14):91-92.
[4] 朱萱萱, 朱芳, 施荣山, 等. 桃仁、防己提取物对大鼠血小板聚集作用的研究[J]. 中医药研究, 2000(3):44-45.
[5] Ge Renyong, Zhou Chuhua She Yunchu..Department of Physiology, Jiangxi College of Traditional Chinese Medicine. INFLUENCES OF STIGMA CROCI AND SEMEN PERSICAE ON FUNCTION OF OVARY-UTERUS IN PSEUDOPREGNANT RATS[J].中医杂志(英文版),1983,(1):23-26.
[6] Xu X, Chen X, Li Y, et al. Cyclooxygenase-2 Regulated by the Nuclear Factor-kappaB Pathway Plays an Important Role in Endometrial Breakdown in a Female Mouse Menstrual-like Model.[J]. Endocrinology, 2013,154(8):2900-2911.
[7] 汪宁, 刘青云, 彭代银, 等. 桃仁不同提取物抗血栓作用的实验研究[J]. 中药材, 2002,(6):414-415.
[8] 耿涛, 谢梅林, 彭少平. 桃仁提取物抗大鼠心肌缺血作用的研究[J]. 苏州大学学报(医学版), 2005,(2):238-240.
[9] Liu L, Duan J A, Tang Y, et al. Taoren-Honghua herb pair and its main components promoting blood circulation through influencing on hemorheology, plasma coagulation and platelet aggregation.[J]. J Ethnopharmacol, 2012,139(2):381-387.
[10] 文川, 徐浩, 黄启福, 等. 活血中药对ApoE基因缺陷小鼠血脂及动脉粥样硬化斑块炎症反应的影响[J]. 中国中西医结合杂志, 2005,(4):345-349.