植物油中真菌毒素检测技术的研究进展
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摘要
真菌毒素是由丝状真菌或霉菌产生的结构多样的小分子次级代谢产物,常见于粮油中,具有致畸性、肝毒性、肾毒性、致癌性、出血性、免疫抑制及破坏生殖系统等毒性。真菌毒素在基体中存在浓度低、种类多、极性范围广、同族类化合物结构类似、性质接近,定性信息缺乏。植物油是易受真菌毒素污染的食品之一,并且含有大量的油脂、脂肪酸和色素,可能增加基质效应,降低灵敏度,并损坏仪器。因此,建立高效样品前处理方法及高灵敏度、高通量、多种真菌毒素检测技术成为植物油基体中真菌毒素准确测定面临的巨大挑战。该文综述了溶剂提取(LLE)、固相萃取(SPE)、凝胶渗透色谱(GPC)、动态共价化学肼(DCHC)、QuEChERS方法在油中真菌毒素前处理中的应用,分析了气相色谱、液相色谱、液相色谱-串联质谱及免疫传感器等检测技术的特点,并对植物油中真菌毒素污染检测的发展应用进行了展望。
Mycotoxins are low-molecular-weight secondary metabolites produced by different fungal species, which can cause teratogenic, hepatotoxic, nephrotoxic, carcinogenic, hemorrhagic, and immunosuppressive effects, and damage the reproductive system in humans and other mammals. Mycotoxins are often present at low concentrations in the matrices and present a branch of compounds, homologs, and isomers. Vegetable oil is one of the top mycotoxin-contaminated foods. Vegetable oil contains lipids, fatty acids, and pigments, which have the potential to increase matrix effects and damage instruments. Therefore, the establishment of high-efficient sample pretreatment methods, and highly sensitive and high-throughput detection techniques are big challenges for the accurate determination of various mycotoxins in vegetable oil. In the present study, the current status and development trends of mycotoxin detection methods in different vegetable oils have been reviewed. Different pretreatment methods such as liquid-liquid extraction, solid phase extraction, gel permeation chromatography, dynamic covalent hydrazine chemistry, and QuEChERS are also summarized. Additionally, characteristics of detection techniques such as gas chromatography, liquid chromatography, tandem mass spectrometry, and immunosensors are discussed.
Mycotoxins are low-molecular-weight secondary metabolites produced by different fungal species, which can cause teratogenic, hepatotoxic, nephrotoxic, carcinogenic, hemorrhagic, and immunosuppressive effects, and damage the reproductive system in humans and other mammals. Mycotoxins are often present at low concentrations in the matrices and present a branch of compounds, homologs, and isomers. Vegetable oil is one of the top mycotoxin-contaminated foods. Vegetable oil contains lipids, fatty acids, and pigments, which have the potential to increase matrix effects and damage instruments. Therefore, the establishment of high-efficient sample pretreatment methods, and highly sensitive and high-throughput detection techniques are big challenges for the accurate determination of various mycotoxins in vegetable oil. In the present study, the current status and development trends of mycotoxin detection methods in different vegetable oils have been reviewed. Different pretreatment methods such as liquid-liquid extraction, solid phase extraction, gel permeation chromatography, dynamic covalent hydrazine chemistry, and QuEChERS are also summarized. Additionally, characteristics of detection techniques such as gas chromatography, liquid chromatography, tandem mass spectrometry, and immunosensors are discussed.
引文
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[69] Xiao M W,Bai X L,Liu Y M,et al.J Chromatogr A,2018,1569:222
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