AFP、GP73、SOD联合检测在原发性肝癌患者诊断中应用价值
摘要
目的:探讨甲胎蛋白(AFP)、高尔基体糖蛋白73(GP73)、超氧化物歧化酶(SOD)联合检测对原发性肝癌的诊断价值。方法:选取收治的原发性肝癌和肝硬化患者各100例(分别为肝癌组和肝硬化组),抽取患者静脉血,采用电化学发光法检测AFP、酶联免疫吸附法检测GP73、黄嘌呤氧化酶法检测SOD,并与100例同期健康体检者(对照组)进行对照,绘制受试者工作特征曲线(ROC),观察AFP、GP73及SOD联合检测对原发性肝癌的诊断价值。结果:肝癌组血清AFP、GP73显著高于肝硬化组和对照组(P<0.05),SOD水平明显低于肝硬化组和对照组(P<0.05)。AFP、GP73及SOD单纯检测在原发性肝癌患者诊断中最佳节点分别为150.29μg/L、214.78μg/L和55.61 IU/m L,联合检测AUC高达0.902,明显高于单纯检测AFP、GP73及SOD的0.726、0.813及0.791。结论:AFP、GP73及SOD联合检测用于原发性肝癌的诊断,可显著提高检出率。
引文
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[14]王丽丽,王立群,高歌.原发性肝癌患者血清中Glypcian-3检测及与AFP联合试验的诊断价值[J].中国实验诊断学,2011,15(6):1054-1056.Wang LL,Wang LQ,Gao G.Glypcian-3 in the detection of hepatocellular carcinoma and the diagnostic value of the Joint detection with AFP[J].Chinese Journal of Laboratory Diagnosis,2011,15(6):1054-1056.
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[16]赵建忠,李智山,许东强,等.甲胎蛋白异质体3和高尔基体蛋白73检测在原发性肝癌诊断中的临床意义[J].中国实验诊断学,2011,15(9):1522-1524.Zhao JZ,Li ZS,Xu DQ,et al.To Evaluate the Capability of Serum Alpha-fetoprotein-L3 and Golgi protein 73 in Diagnosing Primary Liver Cancer[J].Chinese Journal of Laboratory Diagnosis,2011,15(9):1522-1524.
[17]Hann HW,Wang M,Hafner J,et al.Analysis of GP73 in patients with HCC as a function of anti-cancer treatment[J].Cancer Biomark.2010;7(6):269-273.
[18]赵运胜,王猛,李军良,等.血清高尔基体蛋白73、异常凝血酶原、磷脂酰基醇蛋白聚糖-3和甲胎蛋白联合检测对原发性肝癌的早期诊断价值研究[J].中国全科医学,2012,15(32):3730-3733.Zhao YS,Wang M,Li JL,et al.Clinical Significance of Serum GP73,DCP,GPC-3 and AFP Tests in Diagnosis of Primary Hepatic Carcinoma[J].Chinese General Practice,2012,15(32):3730-3733.
[19]Hasegawa K,Suetsugu A,Nakamura M,et al.Imaging NuclearCytoplasmic Dynamics in Primary and Metastatic Colon Cancer in Nude Mice[J].Anticancer Res,2016,36(5):2113-2117.
[20]Abogresha NM,Greish SM,Abdelaziz EZ,et al.Remote effect of kidney ischemia-reperfusion injury on pancreas:role of oxidative stress and mitochondrial apoptosis[J].Arch Med Sci,2016,12(2):252-262.
[21]黄学文,赵琪,陈道桢,等.肝癌患者血清及组织中R O S、T-SOD、Mn SOD水平的测定[J].检验医学,2004,19(5):399-402.Huang XW,Zhao Q,Chen DZ,et al.Determination of reactive oxygen species,total superoxide dismutase and manganese superoxide dismutase in serum and tissue of primary hepatocellular carcinoma patients[J].Laboratory Medicine,2004,19(5):399-402.
[22]杨丽丽,王培胜,滕洪明,等.褐藻多糖硫酸酯诱导肝癌细胞凋亡中氧化应激作用[J].中国细胞生物学学报,2013,35(4):437-441.Yang LL,Wang PS,Teng HM,et al.Oxidative Stress in Apoptosis of Human Hepatocellular Carcinoma SMMC-7721 Cells Induced by Fucoidan[J].Chinese Journal of Cell Biology,2013,35(4):437-441.
[2]陈俊,王利健,骆剑明,等.甲胎蛋白异质体在原发性肝癌中的诊断价值[J].中国卫生检验杂志,2012,22(11):2673-2674.Chen J,Wang LJ,Luo JM,et al.Diagnosis value of alphafetoprotein heterogeneity in primary hepatic carcinoma[J].Chinses Journal of Health Laboratory Technology,2012,22(11):2673-2674.
[3]赵运胜,王猛,崔辰莹,等.评价8种血清标志物在原发性肝癌诊断中的临床价值[J].重庆医学,2014,43(2):214-216.Zhao YS,Wang M,Cui CY,et al.Assessment of clinical values of8 serum markers in diagnosis of primary liver cancer[J].Chongqing Medical Journal,2014,43(2):214-216.
[4]中国抗癌协会肝癌专业委员会,中国抗癌协会临床肿瘤学协作委员会,中华医学会肝病学会肝癌学组.原发性肝癌规范化诊治专家共识[J].临床肿瘤学杂志,2009,14(3):259-269.Chinese Society of Liver Cancer,Chinese Society of Clinical Oncology,Liver Cancer Group,Chinese Society of Hepatology.Experts consensus on standard diagnosis and treatment of primary liver cancer[J].Chinese Clinical Oncology,2009,14(3):259-269.
[5]中国人民共和国卫生部.全国临床检验操作规程[M].东南大学出版社,1991:87.Chinese Ministry of Health. National clinical labo manipulating regulation[M].Nanjing:Southeast University Press,1991:87.
[6]张强.原发性肝癌组织中HMGB1的表达及其与细胞侵袭力关系的研究[J].中国现代普通外科进展,2015,18(6):455-458.Zhang Q.Expression of HMGB1 in hepatocellular carcinoma and its relationship with cell invasive ability[J].Chinese Journal of Current Advances in General Surgery,2015,18(6):455-458.
[7]Dayton TL,Gocheva V,Miller KM,et al.Germline loss of PKM2promotes metabolic distress and hepatocellular carcinoma[J].Genes Dev,2016,30(9):1020-1033.
[8]冯众一,高鹏,谭文翔,等.原发性肝癌TACE介入治疗前后血清VEGF和b FGF水平变化及临床意义[J].中国现代普通外科进展,2015,18(2):154-155.Feng ZY,Gao P,Tan WX,et al.Changes in VEGF and b FGF before and after TACE treatment for primary cancer and their clinical significance[J].Chinese Journal of Current Advances in General Surgery,2015,18(2):154-155.
[9]徐楠,李旭.原发性肝癌肿瘤标志物研究进展[J].安徽医科大学学报,2011,46(4):396-399.Xu N,Li X.Research progress of tumor markers for primary liver cancer[J].Acta Universitis Medicinalis Anhui,2011,46(4):396-399.
[10]Shastri A,Msaouel P,Montagna C,et al.Primary Hepatic Small Cell Carcinoma:Two Case Reports,Molecular Characterization and Pooled Analysis of Known Clinical Data[J].Anticancer Res,2016,36(1):271-277.
[11]贾保昌,罗小玲,梁嵘,等.联合检测血清GP73和AFP对原发性肝癌诊断价值的探讨[J].中华肿瘤防治杂志,2012,19(11):832-835.Jia BC,Luo XL,Liang R,et al.Diagnostic value of serum GP73and AFP detection in primary hepatic carcinoma[J].Chinese Journal of Cancer Prevention and Treatment,2012,19(11):832-835.
[12]秦继宝,薄维波.血清AFP、TK1、DKK1联合检测对原发性肝癌的诊断价值[J].临床检验杂志,2014,32(7):545-547.Qin XB,Bo WB.Combined detection of AFP,TK1 and DKK1 in diagnosis of primary hepatocellular carcinoma[J].Chinese Journal of Clinical Laboratory Science,2014,32(7):545-547.
[13]孔祥亘,韩绍磊,郑昭敏,等.高尔基体蛋白73联合甲胎蛋白异质体在原发性肝癌诊断中的价值[J].山东大学学报:医学版,2011,49(4):110-114.Kong XG,Han SL,Zheng SM,et al.Values of Golgi protein 73and Alpha-fetoprotein-L3 in the diagnosis of primary hepatic carcinoma[J].Journal of Shandong University:Health Sciences,2011,49(4):110-114.
[14]王丽丽,王立群,高歌.原发性肝癌患者血清中Glypcian-3检测及与AFP联合试验的诊断价值[J].中国实验诊断学,2011,15(6):1054-1056.Wang LL,Wang LQ,Gao G.Glypcian-3 in the detection of hepatocellular carcinoma and the diagnostic value of the Joint detection with AFP[J].Chinese Journal of Laboratory Diagnosis,2011,15(6):1054-1056.
[15]Br?ker ME,Ijzermans JN,Witjes CD,et al.The predictive value of Golgi protein 73 in differentiating benign from malignant liver tumors[J].PLo S One,2014,9(7):e100187.doi:10.1371/journal.pone.0100187.
[16]赵建忠,李智山,许东强,等.甲胎蛋白异质体3和高尔基体蛋白73检测在原发性肝癌诊断中的临床意义[J].中国实验诊断学,2011,15(9):1522-1524.Zhao JZ,Li ZS,Xu DQ,et al.To Evaluate the Capability of Serum Alpha-fetoprotein-L3 and Golgi protein 73 in Diagnosing Primary Liver Cancer[J].Chinese Journal of Laboratory Diagnosis,2011,15(9):1522-1524.
[17]Hann HW,Wang M,Hafner J,et al.Analysis of GP73 in patients with HCC as a function of anti-cancer treatment[J].Cancer Biomark.2010;7(6):269-273.
[18]赵运胜,王猛,李军良,等.血清高尔基体蛋白73、异常凝血酶原、磷脂酰基醇蛋白聚糖-3和甲胎蛋白联合检测对原发性肝癌的早期诊断价值研究[J].中国全科医学,2012,15(32):3730-3733.Zhao YS,Wang M,Li JL,et al.Clinical Significance of Serum GP73,DCP,GPC-3 and AFP Tests in Diagnosis of Primary Hepatic Carcinoma[J].Chinese General Practice,2012,15(32):3730-3733.
[19]Hasegawa K,Suetsugu A,Nakamura M,et al.Imaging NuclearCytoplasmic Dynamics in Primary and Metastatic Colon Cancer in Nude Mice[J].Anticancer Res,2016,36(5):2113-2117.
[20]Abogresha NM,Greish SM,Abdelaziz EZ,et al.Remote effect of kidney ischemia-reperfusion injury on pancreas:role of oxidative stress and mitochondrial apoptosis[J].Arch Med Sci,2016,12(2):252-262.
[21]黄学文,赵琪,陈道桢,等.肝癌患者血清及组织中R O S、T-SOD、Mn SOD水平的测定[J].检验医学,2004,19(5):399-402.Huang XW,Zhao Q,Chen DZ,et al.Determination of reactive oxygen species,total superoxide dismutase and manganese superoxide dismutase in serum and tissue of primary hepatocellular carcinoma patients[J].Laboratory Medicine,2004,19(5):399-402.
[22]杨丽丽,王培胜,滕洪明,等.褐藻多糖硫酸酯诱导肝癌细胞凋亡中氧化应激作用[J].中国细胞生物学学报,2013,35(4):437-441.Yang LL,Wang PS,Teng HM,et al.Oxidative Stress in Apoptosis of Human Hepatocellular Carcinoma SMMC-7721 Cells Induced by Fucoidan[J].Chinese Journal of Cell Biology,2013,35(4):437-441.