碳钢表面混合与单种细菌腐蚀作用对比研究
|
摘要
在人工配水环境下,设置空白、大肠埃希氏菌(E.coli)、荧光假单胞菌(P.fluorescens)和混合细菌(E.coli-P.fluorescens)工况,对碳钢试片进行腐蚀实验。通过腐蚀失重法、电化学测试、扫描电镜(SEM)、原子力显微镜(AFM)、微生物活性ATP、微电极、X射线衍射(XRD)和红外光谱等技术研究了单种细菌E.coli、P.fluorescens和混合细菌E.coli-P.fluorescens在碳钢表面的腐蚀行为及其变化。实验结果表明,与空白工况相比,碳钢的腐蚀速率在E.coli工况下增加了27.01%,P.fluorescens工况下降低了48.92%,E.coli-P.fluorescens工况下降低了37.46%。混合细菌工况下,碳钢界面形成了以P.fluorescens为主的网状生物膜结构,腐蚀产物类型、官能团、电化学行为、溶解氧性质等也更接近P.fluorescens工况,说明在两种细菌共同作用中,P.fluorescens占主导地位。
The purpose of the present work is to simulate and comparatively study the microbiologically influenced corrosion on the carbon steel surface. In the artificial water environment, blank, E. coli, P. fluorescens and E. coli-P. fluorescens conditions were set. Carbon steel coupons were placed in the four experimental device and corrosion experiments were carried out. Corrosion behavior and the changes of carbon steel inf-luenced by single bacteria and mixed bacteria were investigated by corrosion weight loss method, electrochemical measurement, AFM, SEM, ATP, microelectrode technology, X-ray diffraction and infrared spectroscopy. The experimental results showed that compared with the average corrosion rate in the blank condition, the E. coli condition increases by 27.01%, the P. fluorescens condition and E. coli-P. fluorescens condition decrease by 48.92% and 37.46%, respectively. In the mixed bacteria condition, there forms a reticular biofilm structure dominated by P. fluorescens at the carbon steel interface, and the corrosion product types, functional groups, electrochemical behavior, and dissolved oxygen are more similar to those under the P. fluorescens condition. This indicated that P. fluorescens exerts the dominant role in the interaction between the two kinds of bacteria.
The purpose of the present work is to simulate and comparatively study the microbiologically influenced corrosion on the carbon steel surface. In the artificial water environment, blank, E. coli, P. fluorescens and E. coli-P. fluorescens conditions were set. Carbon steel coupons were placed in the four experimental device and corrosion experiments were carried out. Corrosion behavior and the changes of carbon steel inf-luenced by single bacteria and mixed bacteria were investigated by corrosion weight loss method, electrochemical measurement, AFM, SEM, ATP, microelectrode technology, X-ray diffraction and infrared spectroscopy. The experimental results showed that compared with the average corrosion rate in the blank condition, the E. coli condition increases by 27.01%, the P. fluorescens condition and E. coli-P. fluorescens condition decrease by 48.92% and 37.46%, respectively. In the mixed bacteria condition, there forms a reticular biofilm structure dominated by P. fluorescens at the carbon steel interface, and the corrosion product types, functional groups, electrochemical behavior, and dissolved oxygen are more similar to those under the P. fluorescens condition. This indicated that P. fluorescens exerts the dominant role in the interaction between the two kinds of bacteria.
引文
1 Basheer R,Ganga G,Chandran R K,et al.Applied Microbiology & Biotechnology,2013,97(12),5615.
2 Castaneda H,Benetton X D.Corrosion Science,2008,50(4),1169.
3 Li H,Zhou E,Ren Y,et al.Corrosion Science,2016,111,811.
4 Xu D,Xia J,Zhou E,et al.Bioelectrochemistry,2017,113,1.
5 Hall-Stoodley L,Costerton J W.Nature Reviews Microbiology,2004,2(2),95.
6 Sun X J,Gao C H,Huang Q Y,et al.Journal of Agricultural Resources and Environment,2017,34(1),6 (in Chinese).孙晓洁,高春辉,黄巧云,等. 农业资源与环境学报,2017,34(1),6.
7 Flemming H C,Wingender J.Nature Reviews Microbiology,2010,8(9),623.
8 Ping L.Resarch of structure characteristics and corrosion property of microbial communities in a simulated reclaimed water distribution system.Master’s Thesis,Beijing University of Civil Engineering and Architecture,China,2016(in Chinese).彭力.再生水模拟管网中微生物群落结构特征及其腐蚀相关性研究.硕士学位论文,北京建筑大学,2016.
9 Dec W,Jaworska-Kik M,Simka W,et al.Materials & Corrosion,2017,69,1.
10 Liu H,Fu C,Gu T,et al.Corrosion Science,2015,100,484.
11 Zhang H,Tian Y,Wan J,et al.Applied Surface Science,2015,357,236.
12 San N O,Nazir H,D?nmez G.Corrosion Science,2012,65(4),113.
13 Duan Y,Li S M,Du J,et al.Acta Physico-Chimica Sinica,2010,26(12),3203(in Chinese).段冶,李松梅,杜娟,等.物理化学学报,2010,26(12),3203.
14 Xu P,Zhai Y J,Wang J,et al.Corrosion Science and Protection Techno-logy,2016,28(4),356(in Chinese).许萍,翟羽佳,王婧,等.腐蚀科学与防护技术,2016,28(4),356.
15 Li J,Xu Z Y,Li J Y,et al.Acta Physico-Chimica Sinica,2010,26(10),2638(in Chinese).李进,许兆义,李久义,等.物理化学学报,2010,26(10),2638.
16 Lin J,Yan Y G,Chen G Z,et al.Journal of Electrochemistry,2006,12(1),93(in Chinese).林晶,阎永贵,陈光章,等.电化学,2006,12(1),93.
17 Wu F.Effect of concentration rate of power plant circulating cooling water on SRB biofilm corrosion to metal.Master’s Thesis,Beijing Jiaotong University,China,2016 (in Chinese).吴芳芳.发电厂循环水浓缩倍率对不锈钢表面SRB生物膜腐蚀的影响研究.硕士学位论文,北京交通大学,2016.
18 Santosh Kr.Karn,Xin Z,Duan J Z.In:2014 Convention on Corrosion and Protection of Marine Material.China,2014.
19 Yin L,Zhao D,Zhang S J,et al.Chinese Journal of Environmental Engineering,2016,10(10),5453(in Chinese).尹朗,赵丹,张素佳,等.环境工程学报,2016,10(10),5453.
20 Xu P,Gao F,Zhai Y J,et al.Surface Technology,2017,46(7),13(in Chinese).许萍,高飞,翟羽佳,等.表面技术,2017,46(7),13.
21 Yang S S,Huang Q Y,Cai P.Chinese Journal of Biotechnology,2017,33(9),1399(in Chinese).杨闪闪,黄巧云,蔡鹏.生物工程学报,2017,33(9),1399.
22 Liu H,Gu T,Asif M,et al.Corrosion Science,2017,114,102.
23 Ke R.International Journal of Electrochemical Science,2016,11(9),7461.
24 Xu C M,Zhang X,Luo L H.Materialy Protection,2017,50(6),40(in Chinese).胥聪敏,张璇,罗立辉.材料保护,2017,50(6),40.
25 Zhao Y L,Yang H W,Song H L,et al.Microbiology China,2017,44(11),2714(in Chinese).赵奕良,杨慧文,宋浩亮,等.微生物学通报,2017,44(11),2714.
26 Li L,Zhang Q Q,Ma B.Journal of Hydroelectric Engineering,2017,36(1),34(in Chinese).李玲,张晴晴,马波.水力发电学报,2017,36(1),34.
27 Duan D X,Chen X G,Lin C G.Journal of Chinese Society for Corrosion and Protection,2011,31(6),453(in Chinese).段东霞,陈西广,蔺存国.中国腐蚀与防护学报,2011,31(6),453.
28 Liu H W,Liu H F.Journal of Chinese Society for Corrosion and Protection,2017,37(3),195(in Chinese).刘宏伟,刘宏芳.中国腐蚀与防护学报,2017,37(3),195.
29 Liu H,Gu T,Zhang G,et al.Corrosion Science,2016,102,93.
30 Welbourn R J L,Truscott C L,Skoda M,et al.Corrosion Science,2016,115,68.
31 Wu T Q,Yang P,Zhang M D,et al.Journal of Chinese Society for Corrosion and Protection,2014,34(4),353(in Chinese).吴堂清,杨圃,张明德,等.中国腐蚀与防护学报,2014,34(4),353.
32 Dong Z H,Liu T,Liu H F.Biofouling,2011,27(5),487.
2 Castaneda H,Benetton X D.Corrosion Science,2008,50(4),1169.
3 Li H,Zhou E,Ren Y,et al.Corrosion Science,2016,111,811.
4 Xu D,Xia J,Zhou E,et al.Bioelectrochemistry,2017,113,1.
5 Hall-Stoodley L,Costerton J W.Nature Reviews Microbiology,2004,2(2),95.
6 Sun X J,Gao C H,Huang Q Y,et al.Journal of Agricultural Resources and Environment,2017,34(1),6 (in Chinese).孙晓洁,高春辉,黄巧云,等. 农业资源与环境学报,2017,34(1),6.
7 Flemming H C,Wingender J.Nature Reviews Microbiology,2010,8(9),623.
8 Ping L.Resarch of structure characteristics and corrosion property of microbial communities in a simulated reclaimed water distribution system.Master’s Thesis,Beijing University of Civil Engineering and Architecture,China,2016(in Chinese).彭力.再生水模拟管网中微生物群落结构特征及其腐蚀相关性研究.硕士学位论文,北京建筑大学,2016.
9 Dec W,Jaworska-Kik M,Simka W,et al.Materials & Corrosion,2017,69,1.
10 Liu H,Fu C,Gu T,et al.Corrosion Science,2015,100,484.
11 Zhang H,Tian Y,Wan J,et al.Applied Surface Science,2015,357,236.
12 San N O,Nazir H,D?nmez G.Corrosion Science,2012,65(4),113.
13 Duan Y,Li S M,Du J,et al.Acta Physico-Chimica Sinica,2010,26(12),3203(in Chinese).段冶,李松梅,杜娟,等.物理化学学报,2010,26(12),3203.
14 Xu P,Zhai Y J,Wang J,et al.Corrosion Science and Protection Techno-logy,2016,28(4),356(in Chinese).许萍,翟羽佳,王婧,等.腐蚀科学与防护技术,2016,28(4),356.
15 Li J,Xu Z Y,Li J Y,et al.Acta Physico-Chimica Sinica,2010,26(10),2638(in Chinese).李进,许兆义,李久义,等.物理化学学报,2010,26(10),2638.
16 Lin J,Yan Y G,Chen G Z,et al.Journal of Electrochemistry,2006,12(1),93(in Chinese).林晶,阎永贵,陈光章,等.电化学,2006,12(1),93.
17 Wu F.Effect of concentration rate of power plant circulating cooling water on SRB biofilm corrosion to metal.Master’s Thesis,Beijing Jiaotong University,China,2016 (in Chinese).吴芳芳.发电厂循环水浓缩倍率对不锈钢表面SRB生物膜腐蚀的影响研究.硕士学位论文,北京交通大学,2016.
18 Santosh Kr.Karn,Xin Z,Duan J Z.In:2014 Convention on Corrosion and Protection of Marine Material.China,2014.
19 Yin L,Zhao D,Zhang S J,et al.Chinese Journal of Environmental Engineering,2016,10(10),5453(in Chinese).尹朗,赵丹,张素佳,等.环境工程学报,2016,10(10),5453.
20 Xu P,Gao F,Zhai Y J,et al.Surface Technology,2017,46(7),13(in Chinese).许萍,高飞,翟羽佳,等.表面技术,2017,46(7),13.
21 Yang S S,Huang Q Y,Cai P.Chinese Journal of Biotechnology,2017,33(9),1399(in Chinese).杨闪闪,黄巧云,蔡鹏.生物工程学报,2017,33(9),1399.
22 Liu H,Gu T,Asif M,et al.Corrosion Science,2017,114,102.
23 Ke R.International Journal of Electrochemical Science,2016,11(9),7461.
24 Xu C M,Zhang X,Luo L H.Materialy Protection,2017,50(6),40(in Chinese).胥聪敏,张璇,罗立辉.材料保护,2017,50(6),40.
25 Zhao Y L,Yang H W,Song H L,et al.Microbiology China,2017,44(11),2714(in Chinese).赵奕良,杨慧文,宋浩亮,等.微生物学通报,2017,44(11),2714.
26 Li L,Zhang Q Q,Ma B.Journal of Hydroelectric Engineering,2017,36(1),34(in Chinese).李玲,张晴晴,马波.水力发电学报,2017,36(1),34.
27 Duan D X,Chen X G,Lin C G.Journal of Chinese Society for Corrosion and Protection,2011,31(6),453(in Chinese).段东霞,陈西广,蔺存国.中国腐蚀与防护学报,2011,31(6),453.
28 Liu H W,Liu H F.Journal of Chinese Society for Corrosion and Protection,2017,37(3),195(in Chinese).刘宏伟,刘宏芳.中国腐蚀与防护学报,2017,37(3),195.
29 Liu H,Gu T,Zhang G,et al.Corrosion Science,2016,102,93.
30 Welbourn R J L,Truscott C L,Skoda M,et al.Corrosion Science,2016,115,68.
31 Wu T Q,Yang P,Zhang M D,et al.Journal of Chinese Society for Corrosion and Protection,2014,34(4),353(in Chinese).吴堂清,杨圃,张明德,等.中国腐蚀与防护学报,2014,34(4),353.
32 Dong Z H,Liu T,Liu H F.Biofouling,2011,27(5),487.