摘要
为了对河南省猪肺炎支原体的流行情况进行调查,试验从发生呼吸道疾病的肺中分离鉴定猪肺炎支原体,对疑似猪肺炎支原体的肺组织样品通过液体培养传代、固体培养基培养、吉姆萨染色镜检,PCR扩增,并进行测序比对。结果表明,2017年1—12月从河南省猪场发生呼吸困难的猪的实变的肺脏共采集148份,共分离25株猪肺炎支原体,分离率达16.89%。结果说明猪肺炎支原体在河南省发生呼吸道疾病的猪场中感染率较高。
To investigate the prevalence of Mycoplasma hyopneumoniae in Henan province,Mycoplasma hyopneumoniae were isolated and identified from the lungs of respiratory disease.Suspected swine Mycoplasma hyopneumonia lung tissue were cultured by liquid and solid medium,Giemsa staining,PCR amplified,sequenced and blasted.The results showed that from January to December2017,a total of 148 samples from the lungs of dyspnea pigs in Henan province were collected.25 strains Mycoplasma pneumoniae were isolated and identified.The isolation rate was 16.89%.The results explained that the infection rate of Mycoplasma hyopneumoniae was high in swine farms with respiratory diseases in Henan province.
引文
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