牛支原体检测方法研究进展
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摘要
牛支原体在全球范围内分布广泛且具有高度传染性,会引起严重疾病,急需一种快速、准确的诊断方法以预防和控制该病的爆发。综述了牛支原体不同检测技术的研究进展。微生物培养是对支原体的鉴定和诊断的传统方法,而近年来,通过聚合酶链式反应检测牛支原体的研究越来越多。与传统方法相比,聚合酶链式反应(PCR)具有更高的诊断效率、特异性以及敏感性。综合比较发现,每一种检测方法都有利有弊,联合使用各种检测法可互相补充信息,同时结合临床症状更有助于支原体的检测和监控牛群疾病状况。
Mycoplasma bovis is widely distributed and highly infectious in the world,causing serious diseases. A rapid and accurate diagnostic method is urgently needed to prevent and control the outbreak of the disease. In this paper,the research progress of different detection techniques for Mycoplasma bovis was reviewed. The traditional method of identification and diagnosis of mycoplasma is microbial culture,but in recent years,more and more studies on detection of Mycoplasma bovis by polymerase chain reaction( PCR) have been carried out. Compared with traditional methods,polymerase chain reaction has higher diagnostic efficiency,specificity and sensitivity. It is found that each detection method has some advantages and disadvantages. Combining use of various detection methods and clinical symptoms can supplement each other's information and combined with clinical symptoms,it is more conducive to the detection of mycoplasma and monitoring the disease status of herds.
Mycoplasma bovis is widely distributed and highly infectious in the world,causing serious diseases. A rapid and accurate diagnostic method is urgently needed to prevent and control the outbreak of the disease. In this paper,the research progress of different detection techniques for Mycoplasma bovis was reviewed. The traditional method of identification and diagnosis of mycoplasma is microbial culture,but in recent years,more and more studies on detection of Mycoplasma bovis by polymerase chain reaction( PCR) have been carried out. Compared with traditional methods,polymerase chain reaction has higher diagnostic efficiency,specificity and sensitivity. It is found that each detection method has some advantages and disadvantages. Combining use of various detection methods and clinical symptoms can supplement each other's information and combined with clinical symptoms,it is more conducive to the detection of mycoplasma and monitoring the disease status of herds.
引文
[1] NICHOLAS R A J,AYLING R D. Mycoplasma bovis:disease,diagnosis,and control[J]. Research in Veterinary Science,2003,74(2):105-112.
[2] BEIER T,HOTZEL H,LYSNYANSKY I,et al. Intraspecies polymorphism of vsp genes and expression profiles of variable surface protein antigens(Vsps)in field isolates of Mycoplasma bovis[J]. Veterinary Microbiology,1998,63(2/4):189-203.
[3] SUBRAMANIAM S,BERGONIER D,POUMARAT F,et al. Species identification of Mycoplasma bovis and Mycoplasma agalactiae based on the uvr C genesby PCR[J].Mol Cell Probes,1998,12(3):161-169.
[4] PFUTZNER H. Epizootiology of the Mycoplasma bovis infection of cattle[J]. Zentralblatt fur Bakteriologie Supplement,1990,20:394-399.
[5] BUCHVAROVA Y,VESSELINOVA A. On the aetiopathogenesis of Mycoplasma pneumonia in calves[J].Arch Exp Veterinarmed,1989,43(5):685-689.
[6] KAUF A C,ROSENBUSCH R F,PAAPE M J,et al. Innate immune response to intramammary Mycoplasma bovis infection[J]. J Dairy Sci,2007,90(7):3336-3348.
[7] POUMARAT F,LE GRAND D,PHILIPPE S,et al. Efficacy of spectinomy cin against Mycoplasma bovis induced pneumonia in conventionally reared calves[J]. Vet Microbiol,2001,80(1):23-35.
[8] ROBINO P,ALBERTI A,PITTAU M,et al. Genetic and antigenic characterization of the surface lipoprotein P48of Mycoplasma bovis[J]. Veterinary Microbiology,2005,109(3/4):201-209.
[9] GAGEA M I,BATEMAN K G,SHANAHAN R A,et al.Naturally occurring Mycoplasma bovisassociated pneumoni[J]. J Vet Diagn Invest,2006,18(1):29-40.
[10] CONNOR A,MARTIN S W,NAGY E,et al. The relationship between the occurrence of undifferentiated bovine respiratory disease and titer changes to bovine coronavirus and bovine viral diarrhea virus in three Ontario feedlots[J]. Can Vet J,2001,65:137-142.
[11]李媛,董惠,张美晶,等.牛支原体套式PCR检测方法的建立[J].中国畜牧兽医学报,2009,31(9):709-711.
[12] CASWELL J L,ARCHAMBAULT M. Mycoplasma bovis pneumonia in cattle[J]. Animal Health Research Review,2007,8(2):161-186.
[13] THOMAS A,DIZIER I,TROLIN A,et al. Comparison of sampling procedures for isolating pulmonary mycoplasmas in cattle[J]. Veterinary Research Communications,2002,26(5):333-339.
[14] TENK M,BLINT A,STIPKOVITS L,et al. Detection of Mycoplasma bovis with an improved pcrassay[J]. Acta Veterinaria Hungarica,2006,54(4):427-435.
[15] CAI H,BELL-ROGERS P,PARKER L,et al. Development of a real-time PCR for detection of My coplasma bov is in bovine milk and lung samples[J]. J Vet Diagn Invest,2005,17(6):537-545.
[16] SACHSE K,HELBIG J H,LYSNYANSKY I,et al.Epitope mapping of immunogenic and adhesive structures in repetitive domains of Mycoplasma bovis variable surface lipoproteins[J]. Infect Immun,2000,68(2):680-687.
[17]李大伟,黄灿平,张彦明,等.牛支原体、无乳支原体和丝状支原体丝状亚种小克隆三重PCR检测方法的建立及初步应用[J].畜牧兽医学报,2011,42(2):306-310.
[18] PINNOW C C,BUTLER J A,SACHSE K,et al. Detection of Mycoplasma bovis in preservative-treated field milk samples[J]. Journal of Dairy Science,2001,84(7):1640-1645.
[19]冉智光,谢建华,骆璐,等.我国部分地区牛支原体肺炎和关节炎的病原体诊断[J].中国预防兽医学报,2010,32(1):40-43.
[20]胡长敏,石磊,龚瑞,等.牛支原体病研究进展[J].动物医学进展,2009,30(8):73-77.
[21]黄庆华,陈桂蓝.不同临床样品肺炎支原体的分离培养[J].临床检验杂志,1998,16(4):242-243.
[22] WAITE E R,M ARCH J B. Capsular polysaccharide conjugate vaccines against contagious bovine pleuropneumonia:Immune responses and protection in mice[J]. J Comp Pathol,2002,126(2/3):171-182.
[23]石磊,龚瑞,尹争艳,等.肉牛传染性牛支原体肺炎流行的诊断[J].华中农业大学学报,2008,27(5):629-633.
[24] MARCH J B,JEPSON C D,CLARK J R,et al. Phage library screening for the rapid identification and in vivo testing of candidate genes for a DNA vaccine against Mycoplasma mycoides subsp. my coides small colony bioty pe[J]. Infect Immun,2006,74(1):167-174.
[25]辛九庆,李媛,郭丹,等.国内首次从患肺炎的犊牛肺脏中分离到牛支原体[J].中国预防兽医学报,2008,30(9):661-664.
[26] WILSON D J,SKIRPSTUNAS R T,TRUJILLO J D,et al.Unusual history and initial clinical signs of Mycoplasma bovis mastitis and arthritis in first-lactation cows in a closed commercial dairy herd[J]. J Am Vet Med Assoc,2007,230:1519-1523.
[27] DUPUY V,MANSO-SILVAN L,Barbe V,et al. Evolutionary history of contagious bovine pleuropneumonia using next generation sequencing of mycoplasma mycoides subsp mycoides “small colony’[J]. Plos One,2012,7:e46821.
[28] MCVEY D S,KENNEDY M,CHENGAPPA M M. Veterinary Microbiology[M]. 3rd ed. Hoboken:Wiley,2013.
[2] BEIER T,HOTZEL H,LYSNYANSKY I,et al. Intraspecies polymorphism of vsp genes and expression profiles of variable surface protein antigens(Vsps)in field isolates of Mycoplasma bovis[J]. Veterinary Microbiology,1998,63(2/4):189-203.
[3] SUBRAMANIAM S,BERGONIER D,POUMARAT F,et al. Species identification of Mycoplasma bovis and Mycoplasma agalactiae based on the uvr C genesby PCR[J].Mol Cell Probes,1998,12(3):161-169.
[4] PFUTZNER H. Epizootiology of the Mycoplasma bovis infection of cattle[J]. Zentralblatt fur Bakteriologie Supplement,1990,20:394-399.
[5] BUCHVAROVA Y,VESSELINOVA A. On the aetiopathogenesis of Mycoplasma pneumonia in calves[J].Arch Exp Veterinarmed,1989,43(5):685-689.
[6] KAUF A C,ROSENBUSCH R F,PAAPE M J,et al. Innate immune response to intramammary Mycoplasma bovis infection[J]. J Dairy Sci,2007,90(7):3336-3348.
[7] POUMARAT F,LE GRAND D,PHILIPPE S,et al. Efficacy of spectinomy cin against Mycoplasma bovis induced pneumonia in conventionally reared calves[J]. Vet Microbiol,2001,80(1):23-35.
[8] ROBINO P,ALBERTI A,PITTAU M,et al. Genetic and antigenic characterization of the surface lipoprotein P48of Mycoplasma bovis[J]. Veterinary Microbiology,2005,109(3/4):201-209.
[9] GAGEA M I,BATEMAN K G,SHANAHAN R A,et al.Naturally occurring Mycoplasma bovisassociated pneumoni[J]. J Vet Diagn Invest,2006,18(1):29-40.
[10] CONNOR A,MARTIN S W,NAGY E,et al. The relationship between the occurrence of undifferentiated bovine respiratory disease and titer changes to bovine coronavirus and bovine viral diarrhea virus in three Ontario feedlots[J]. Can Vet J,2001,65:137-142.
[11]李媛,董惠,张美晶,等.牛支原体套式PCR检测方法的建立[J].中国畜牧兽医学报,2009,31(9):709-711.
[12] CASWELL J L,ARCHAMBAULT M. Mycoplasma bovis pneumonia in cattle[J]. Animal Health Research Review,2007,8(2):161-186.
[13] THOMAS A,DIZIER I,TROLIN A,et al. Comparison of sampling procedures for isolating pulmonary mycoplasmas in cattle[J]. Veterinary Research Communications,2002,26(5):333-339.
[14] TENK M,BLINT A,STIPKOVITS L,et al. Detection of Mycoplasma bovis with an improved pcrassay[J]. Acta Veterinaria Hungarica,2006,54(4):427-435.
[15] CAI H,BELL-ROGERS P,PARKER L,et al. Development of a real-time PCR for detection of My coplasma bov is in bovine milk and lung samples[J]. J Vet Diagn Invest,2005,17(6):537-545.
[16] SACHSE K,HELBIG J H,LYSNYANSKY I,et al.Epitope mapping of immunogenic and adhesive structures in repetitive domains of Mycoplasma bovis variable surface lipoproteins[J]. Infect Immun,2000,68(2):680-687.
[17]李大伟,黄灿平,张彦明,等.牛支原体、无乳支原体和丝状支原体丝状亚种小克隆三重PCR检测方法的建立及初步应用[J].畜牧兽医学报,2011,42(2):306-310.
[18] PINNOW C C,BUTLER J A,SACHSE K,et al. Detection of Mycoplasma bovis in preservative-treated field milk samples[J]. Journal of Dairy Science,2001,84(7):1640-1645.
[19]冉智光,谢建华,骆璐,等.我国部分地区牛支原体肺炎和关节炎的病原体诊断[J].中国预防兽医学报,2010,32(1):40-43.
[20]胡长敏,石磊,龚瑞,等.牛支原体病研究进展[J].动物医学进展,2009,30(8):73-77.
[21]黄庆华,陈桂蓝.不同临床样品肺炎支原体的分离培养[J].临床检验杂志,1998,16(4):242-243.
[22] WAITE E R,M ARCH J B. Capsular polysaccharide conjugate vaccines against contagious bovine pleuropneumonia:Immune responses and protection in mice[J]. J Comp Pathol,2002,126(2/3):171-182.
[23]石磊,龚瑞,尹争艳,等.肉牛传染性牛支原体肺炎流行的诊断[J].华中农业大学学报,2008,27(5):629-633.
[24] MARCH J B,JEPSON C D,CLARK J R,et al. Phage library screening for the rapid identification and in vivo testing of candidate genes for a DNA vaccine against Mycoplasma mycoides subsp. my coides small colony bioty pe[J]. Infect Immun,2006,74(1):167-174.
[25]辛九庆,李媛,郭丹,等.国内首次从患肺炎的犊牛肺脏中分离到牛支原体[J].中国预防兽医学报,2008,30(9):661-664.
[26] WILSON D J,SKIRPSTUNAS R T,TRUJILLO J D,et al.Unusual history and initial clinical signs of Mycoplasma bovis mastitis and arthritis in first-lactation cows in a closed commercial dairy herd[J]. J Am Vet Med Assoc,2007,230:1519-1523.
[27] DUPUY V,MANSO-SILVAN L,Barbe V,et al. Evolutionary history of contagious bovine pleuropneumonia using next generation sequencing of mycoplasma mycoides subsp mycoides “small colony’[J]. Plos One,2012,7:e46821.
[28] MCVEY D S,KENNEDY M,CHENGAPPA M M. Veterinary Microbiology[M]. 3rd ed. Hoboken:Wiley,2013.