不同熟化时期白木香果皮挥发性成分的研究
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摘要
目的:对白木香果皮6个不同熟化时期的挥发性成分进行分析,以期发现白木香非药用部位中沉香类成分,挖掘白木香果皮潜在价值,为实现白木香非药用部位的可持续开发利用奠定理论基础。方法:本实验采用超声提取技术提取白木香果皮中挥发性成分,借助气相色谱-质谱联用技术(GC-MS)分析不同熟化程度果皮的挥发性成分。结果:共检测到207种化合物,包括萜类、甾类、脂肪酸、生物碱、烷烃及烯烃等;其中,检测到的倍半萜类成分包括桉叶烷型、愈创木烯型和螺旋烷型倍半萜,均为沉香的特征性成分;进一步分析表明白木香果皮熟化的第Ⅲ期倍半萜类成分及单萜相对质量分数最高分别达1.17%、0.66%,而生育酚则在Ⅲ期后下降到检测限下。结论:本研究将为全面合理开发白木香非药用部位及白木香可持续利用奠定基础。
Objective:To discover the agarwood components in non-medicinal parts of Aquilaria sinensis,the volatile components of A.sinensis pericarp in six maturation periods were analyzed.The study aims at discovering the potential value of the pericarp and laying a theoretical foundation for the sustainable development and utilization of the non-medicinal parts of A.sinensis.Methods:In our work,ultrasonic extraction technology was used to extract the volatile components of the peels of A.sinensis.Meanwhile,the gas chromatography-mass spectrometry(GC-MS)was used to analyze the pericarp in different degrees of maturity.Results:207 compounds were detected by GC-MS,including terpenes,steroids,fatty acids,alkaloids,alkanes,olefins and the other ingredients.In addition,characteristic sesquiterpenes of agarwood including eudesmane,guaiane and agarospirane types component were detected.Furthermore,sesquiterpenes and monoterpenoids were mainly accumulated in the third maturity period of A.sinensis pericarp,the content of which was 1.17% and 0.66%,respectively.However,the content of tokoferol was declined to the detection limit after the third maturity period of A.sinensis pericarp.Conclusion:The study will lay a foundation for rational development and sustainable utilization of non-medical parts of A.sinensis.
Objective:To discover the agarwood components in non-medicinal parts of Aquilaria sinensis,the volatile components of A.sinensis pericarp in six maturation periods were analyzed.The study aims at discovering the potential value of the pericarp and laying a theoretical foundation for the sustainable development and utilization of the non-medicinal parts of A.sinensis.Methods:In our work,ultrasonic extraction technology was used to extract the volatile components of the peels of A.sinensis.Meanwhile,the gas chromatography-mass spectrometry(GC-MS)was used to analyze the pericarp in different degrees of maturity.Results:207 compounds were detected by GC-MS,including terpenes,steroids,fatty acids,alkaloids,alkanes,olefins and the other ingredients.In addition,characteristic sesquiterpenes of agarwood including eudesmane,guaiane and agarospirane types component were detected.Furthermore,sesquiterpenes and monoterpenoids were mainly accumulated in the third maturity period of A.sinensis pericarp,the content of which was 1.17% and 0.66%,respectively.However,the content of tokoferol was declined to the detection limit after the third maturity period of A.sinensis pericarp.Conclusion:The study will lay a foundation for rational development and sustainable utilization of non-medical parts of A.sinensis.
引文
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[2]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:185-186.
[3]OKUDERA Y,ITO M.Production of agarwood fragrant constituents in Aquilaria calli and cell suspension cultures[J].Plant Biotechnol J,2009,26(3):307-315.
[4]张争,杨云,魏建和,等.白木香结香机制研究进展及其防御反应诱导结香假说[J].中草药,2010,41(1):156-159.
[5]徐维娜,高晓霞,郭晓玲,等.白木香果皮挥发性成分及抗肿瘤活性的研究[J].中药材,2010,33(11):1736-1740.
[6]张兴,陶美华,陈玉婵,等.白木香果皮中的五个葫芦素类化合物及其细胞毒活性[J].天然产物研究与开发,2014,26(3):354-357.
[7]张兴,陶美华,陈玉婵,等.白木香果皮化学成分及其生物活性研究[J].中草药,2013,44(10):1248-1252.
[8]林峰.白木香果实和花的化学成分研究[D].海口:海南大学,2010.
[9]陈荣荣.不同陈化年份广陈皮中香气活性组分鉴定[D].杭州:浙江大学,2017.
[10]陈聪聪.广陈皮陈化过程中微生物群落多样性解析及代谢物成分变化分析[D].广州:华南理工大学,2017.
[11]陈怀琼.白木香通体结香技术的初步评价[D].北京:北京协和医学院,2011.
[12]孙敬茹,卜俊玲,赵欢,等.四种姜黄属药用植物根茎和块根挥发性代谢物的多元数据比较分析[J].药学学报,2018,53(8):1215-1224.
[13]梅文莉,林峰,戴好富.白木香花和果实挥发油化学成分的GC-MS分析[G].海南省首届黎族药物学术交流会论文汇编,2008.
[14]ZHANG Z,ZHANG X,YANG Y,et al.Hydrogen peroxide induces vessel occlusions and stimulates sesquiterpenes accumulation in stems of Aquilaria sinensis[J].Plant Growth Regul,2014,72(1):81-87.
[15]CHEN HQ,WEI JH,YANG JS,et al.Chemical constituents of agarwood originating from the endemic genus Aquilaria plants[J].Chem Biodivers,2012,9(2):236-250.
[16]JAYACHANDRAN K,SEKAR I,PARTHIBAN KT,et al.Analysis of different grades of agarwood(Aquilaria malaccensis Lamk.)oil through GC-MS[J].IJNPR,2015,5(1):44-47.
[17]杨峻山.沉香化学成分的研究概况[J].天然产物研究与开发,1997,10(1):99-103.
[18]李浩华,章卫民,陈玉婵,等.白木香果皮提取物清除DPPH自由基能力及抑制酪氨酸酶活性的研究[J].中国民族民间医药,2014,23(8):36-38.
[19]夏铮南,王文君.香料与香精[M].北京:中国物资出版社,1998:173-175,217-227.
[20]孙宝国,郑福平,刘玉平.香料与香精[M].北京:中国石化出版社,2000:54-55,270-274.