玉米大斑病抗性基因SNP基因芯片分析
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摘要
利用SNP基因芯片(56 110个SNP)对3对玉米大斑病Ht3近等基因系进行检测分析。借助生物信息学和比较基因组学方法,确定了29个相关数量抗性候选基因,均分布于2、3、7、9号染色体;14个候选基因与各类酶活性、能源合成、金属离子、DNA合成修饰、抗逆等功能相关。其中,GRMZM2G058197位于7号染色体bin7.04区域166087520-166091392,与玉米大斑病质量抗性基因Ht3基因位置相吻合。
In this paper, we analyzed three pairs of near-isogenic lines related with maize northern corn leaf blight(NCLB) using SNP gene chip(56,110 SNPs). Through bioinformatics and comparative genomics analysis, 29 candidate genes were identified, which were distributed on chromosomes 2, 3, 7 and 9, respectively. Among them,14 had functional annotation, which were associated with many functions, such as enzyme activities, energy synthesis, metal ions binding, DNA modification, stress resistance. In particular, GRMZM2 G058197 is located at bin 7.04,within the interval of 166087520 and 166091392, where is the location that Ht3 was preliminarily identified. The results would provide theoretical and data support for further NCLB research.
In this paper, we analyzed three pairs of near-isogenic lines related with maize northern corn leaf blight(NCLB) using SNP gene chip(56,110 SNPs). Through bioinformatics and comparative genomics analysis, 29 candidate genes were identified, which were distributed on chromosomes 2, 3, 7 and 9, respectively. Among them,14 had functional annotation, which were associated with many functions, such as enzyme activities, energy synthesis, metal ions binding, DNA modification, stress resistance. In particular, GRMZM2 G058197 is located at bin 7.04,within the interval of 166087520 and 166091392, where is the location that Ht3 was preliminarily identified. The results would provide theoretical and data support for further NCLB research.
引文
[1]Hooker A L.Monogenic resistance of Zea mays L.to Helminthosporium turcicum[J].Crop Sci.,1963,3:381-383.
[2]Hooker A L.A second major gene locus in corn for chlorotic-lesion resistance to Helminthosporium turcicum[J].Crop Sci.,1977,17:132-135.
[3]Hooker A L.Resistance to Helminthosporium turcicum from Tripsacum floridanum incorporated into corn[J].Maize Genet Cooperation News Lett.,1981,55:87-88.
[4]Carson M L.A new gene in maize conferring the chlortic halo reaction to infection by exserohilum-turcicun[J].Plant Disease.,1995,79:717-720.
[5]Raymundo A D,Hooker A L,Perkins J M.Effect of gene Ht N Oil development corn leaf blight epidemics[J].Plant Disease,1981,65:327-330.
[6]Ogliari J B,Guimaraes M A,Camargo L E A.Chromosomal locations of the maize(Zea mays L.)Ht P and rt genes that confer resistance to Exserohilum turcicum[J].Genetics and Molecular Biology,2007,30(3):630-634.
[7]Ogliari J B,Guimaraes M A,Geraldi I O.New resistance genes in the Zea mays Exserohilu turcicum pathosystem[J].Genetics and Molecular Biology,2005,28:435-439.
[8]Couture R M,Routley D G,Dunn G M.Role of cylie hydroxamic acids in monogenic resistance of maize to Helminthosporium turcicum[J].Physiological Plant Pathology,1971,1:51.
[9]Hurni S,Scheuermann D,Krattinger S G,et al.The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wallassociated receptor-like kinase[J].Proc Natl Acad Sci.,2015,112:8781-8785.
[10]张秀霞,高增贵,周晓锟,等.东北地区玉米大斑病菌生理分化研究[J].华北农学报,2012,27(3):227-230.Zhang X X,Gao Z G,Zhou X K,et al.Identification of physiological races of exserohilum turcicum in northeastern China[J].Acta Agriculturae Boreali-Sinica,2012,27(3):227-230.(in Chinese)
[11]赵辉,高增贵,张小飞,等.我国玉米大斑病菌生理小种种群动态分析[J].沈阳农业大学学报,2008(39):551-555.Zhao H,Gao Z G,Zhang X F,et al.Population of physiological races of setosphaeria turcica and its dynamic analysis in China[J].Journal of Shenyang Agricultural University,2008(39):551-555.(in Chinese)
[12]Simcox K D,Jeflrey L,Bennetzen.Mapping the Ht N resistance gene to the long arm of chromosome 8[J].Maize Genetic Cooperator Newsletter,1993,67:118-119.
[13]Carson M L,Vandyke C G.Of light and temperature on expression of partial resistance of maize to Helminthosporium-turcicum[J].Plant Disease,1994,78:519-522.
[14]Brown A F,Juvik J A,Pataky J K.Quantitative trait loci in sweet corn associated with partial resistance to stewart′s wilt,northern corn leaf blight,and common rust[J].Phytopathology,2001,91:293-300.
[15]Chung C L,Jamann T,Longfellow J,et al.Characterization and fine-mapping of a resistance locus for northern leaf blight in maize bin 8.06[J].Theoretical and applied genetics,2010,121:205-227.
[16]Chung C L,Poland J,Kump K,et al.Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize[J].Theoretical and Applied Genetics,2011,123:307-326.
[17]Zwonitzer J C,Coles N D,Krakowsky M D,et al.Mapping resistance quantitative trait Loci for three foliar diseases in a maize recombinant inbred line population-evidence for multiple disease resistance[J].Phytopathology,2010,100:72-79.
[18]Welz H G,Xia X C,Bassetti P,et al.QTLs for resistance to Setosphaeria turcica in an early maturing Dentx Flint maize population[J].Theoretical and Applied Genetics,1999,99:649-655.
[19]Schechert A W,Welz H G,Geiger H H.QTL for Resistance to Setosphaeria turcica in Tropical African maize[J].Crop Science,1999,39:514-523.
[20]Jamann T M,Poland J A,Kolkman J M,et al.Unraveling genomic complexity at a quantitative disease resistance locus in maize[J].Genetics,2014,198:333-344.
[21]Balint-Kurti P J,Yang J,Van Esbroeck G,et al.Use of a maize advanced intercross line for mapping of qtl for northern leaf blight resistance and multiple disease resistance[J].Crop Science,2010,50:458-466.
[22]郑祖平,刘小红,黄玉碧,等.玉米大斑病抗性基因的QTL定位[J].西南农业学报,2007,22(4):634-637.Zheng Z P,Liu X H,Huang Y B,et al.QTL mapping of maize leaf spot resistance gene[J].Southwest China Journal of Agricultural Sciences,2007,22(4):634-637.(in Chinese)
[23]Freymark P J,Lee M,Woodman W L,et al.Quantitative and qualitative trait loci affecting host-plant response to Exserohilum turcicum in maize(Zea mays L.)[J].Theoretical and applied genetics,1993,87:537-544.
[24]Asea G,Vivek B S,Bigirwa G,et al.Validation of consensus quantitative trait loci associated with resistance to multiple foliar pathogens of maize[J].Phytopathology,2009,99:540-547.
[25]Chen G S,Wang X M,Long S S,et al.Mapping of QTL conferring resistance to northern corn leaf blight using high-density SNPs in maize[J].Molecular Breeding January,2016,36:4.
[26]Ding J Q,Farhan Ali,Chen G S,et al.Genome-wide association mapping reveals novel sources of resistance to northern corn leaf blight in maize[J].BMC Plant Biology,2015,15:206-216.
[27]Jing M,Zhang C Y,Hussain K,et al.Pyramiding resistance genes to northern leaf blight and head smut in maize[J].International Journal of Agriculture&Biology,2012,3:430-434.
[28]Murray M G,Thompson W F.Rapid isolation of high molecular weight plant DNA[J].Nucleic Acids Res,1980,8:4321-4326.
[29]Weng J F,Xie C X,Hao Z F,et al.Genome-wide association study identifies candidate genes that affect plant height in Chinese elite maize(Zea mays L.)inbred lines[J].PLo S One,2011,6:29229.
[30]Jesse A P,Peter J B,Edward S B,et al.Genome-wide nested association mapping of quantitative resistance to northern leaf blight in maize[J].PNAS,2011,108(17):6893-6898.
[31]Zhou J,Loh Y T,Bressan R A,et al.The tomato gene Pti1 encodes a serine/threonine kinase that is phosphorylated by Pto and is involved in the hypersensitive response[J].Cell,1995,83:925-935.
[32]Laplante A F,Moulin V,Auger F A,et al.Expression of heat shock proteins in mouse skin during wound healing[J].Journal of Histochemistry and Cytochemistry,1998,46(11):1291-1301.
[33]Jump up,De Maio A.Heat shock proteins:facts,thoughts,and dreams[J].Shock,1999,11(1):1-12.
[35]Ali F,Yan J B.The phenomenon of disease resistance in maize and the role of molecular breeding in defending against global threat[J].Integrated Plant Biol,2012,55:134-151.
[36]Horim L,Ok-Kyong C,Jen S.Stem-cell-triggered immunity through by CLV3p-FLS2 signaling[J].Nature,2011,473:376-379.
[2]Hooker A L.A second major gene locus in corn for chlorotic-lesion resistance to Helminthosporium turcicum[J].Crop Sci.,1977,17:132-135.
[3]Hooker A L.Resistance to Helminthosporium turcicum from Tripsacum floridanum incorporated into corn[J].Maize Genet Cooperation News Lett.,1981,55:87-88.
[4]Carson M L.A new gene in maize conferring the chlortic halo reaction to infection by exserohilum-turcicun[J].Plant Disease.,1995,79:717-720.
[5]Raymundo A D,Hooker A L,Perkins J M.Effect of gene Ht N Oil development corn leaf blight epidemics[J].Plant Disease,1981,65:327-330.
[6]Ogliari J B,Guimaraes M A,Camargo L E A.Chromosomal locations of the maize(Zea mays L.)Ht P and rt genes that confer resistance to Exserohilum turcicum[J].Genetics and Molecular Biology,2007,30(3):630-634.
[7]Ogliari J B,Guimaraes M A,Geraldi I O.New resistance genes in the Zea mays Exserohilu turcicum pathosystem[J].Genetics and Molecular Biology,2005,28:435-439.
[8]Couture R M,Routley D G,Dunn G M.Role of cylie hydroxamic acids in monogenic resistance of maize to Helminthosporium turcicum[J].Physiological Plant Pathology,1971,1:51.
[9]Hurni S,Scheuermann D,Krattinger S G,et al.The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wallassociated receptor-like kinase[J].Proc Natl Acad Sci.,2015,112:8781-8785.
[10]张秀霞,高增贵,周晓锟,等.东北地区玉米大斑病菌生理分化研究[J].华北农学报,2012,27(3):227-230.Zhang X X,Gao Z G,Zhou X K,et al.Identification of physiological races of exserohilum turcicum in northeastern China[J].Acta Agriculturae Boreali-Sinica,2012,27(3):227-230.(in Chinese)
[11]赵辉,高增贵,张小飞,等.我国玉米大斑病菌生理小种种群动态分析[J].沈阳农业大学学报,2008(39):551-555.Zhao H,Gao Z G,Zhang X F,et al.Population of physiological races of setosphaeria turcica and its dynamic analysis in China[J].Journal of Shenyang Agricultural University,2008(39):551-555.(in Chinese)
[12]Simcox K D,Jeflrey L,Bennetzen.Mapping the Ht N resistance gene to the long arm of chromosome 8[J].Maize Genetic Cooperator Newsletter,1993,67:118-119.
[13]Carson M L,Vandyke C G.Of light and temperature on expression of partial resistance of maize to Helminthosporium-turcicum[J].Plant Disease,1994,78:519-522.
[14]Brown A F,Juvik J A,Pataky J K.Quantitative trait loci in sweet corn associated with partial resistance to stewart′s wilt,northern corn leaf blight,and common rust[J].Phytopathology,2001,91:293-300.
[15]Chung C L,Jamann T,Longfellow J,et al.Characterization and fine-mapping of a resistance locus for northern leaf blight in maize bin 8.06[J].Theoretical and applied genetics,2010,121:205-227.
[16]Chung C L,Poland J,Kump K,et al.Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize[J].Theoretical and Applied Genetics,2011,123:307-326.
[17]Zwonitzer J C,Coles N D,Krakowsky M D,et al.Mapping resistance quantitative trait Loci for three foliar diseases in a maize recombinant inbred line population-evidence for multiple disease resistance[J].Phytopathology,2010,100:72-79.
[18]Welz H G,Xia X C,Bassetti P,et al.QTLs for resistance to Setosphaeria turcica in an early maturing Dentx Flint maize population[J].Theoretical and Applied Genetics,1999,99:649-655.
[19]Schechert A W,Welz H G,Geiger H H.QTL for Resistance to Setosphaeria turcica in Tropical African maize[J].Crop Science,1999,39:514-523.
[20]Jamann T M,Poland J A,Kolkman J M,et al.Unraveling genomic complexity at a quantitative disease resistance locus in maize[J].Genetics,2014,198:333-344.
[21]Balint-Kurti P J,Yang J,Van Esbroeck G,et al.Use of a maize advanced intercross line for mapping of qtl for northern leaf blight resistance and multiple disease resistance[J].Crop Science,2010,50:458-466.
[22]郑祖平,刘小红,黄玉碧,等.玉米大斑病抗性基因的QTL定位[J].西南农业学报,2007,22(4):634-637.Zheng Z P,Liu X H,Huang Y B,et al.QTL mapping of maize leaf spot resistance gene[J].Southwest China Journal of Agricultural Sciences,2007,22(4):634-637.(in Chinese)
[23]Freymark P J,Lee M,Woodman W L,et al.Quantitative and qualitative trait loci affecting host-plant response to Exserohilum turcicum in maize(Zea mays L.)[J].Theoretical and applied genetics,1993,87:537-544.
[24]Asea G,Vivek B S,Bigirwa G,et al.Validation of consensus quantitative trait loci associated with resistance to multiple foliar pathogens of maize[J].Phytopathology,2009,99:540-547.
[25]Chen G S,Wang X M,Long S S,et al.Mapping of QTL conferring resistance to northern corn leaf blight using high-density SNPs in maize[J].Molecular Breeding January,2016,36:4.
[26]Ding J Q,Farhan Ali,Chen G S,et al.Genome-wide association mapping reveals novel sources of resistance to northern corn leaf blight in maize[J].BMC Plant Biology,2015,15:206-216.
[27]Jing M,Zhang C Y,Hussain K,et al.Pyramiding resistance genes to northern leaf blight and head smut in maize[J].International Journal of Agriculture&Biology,2012,3:430-434.
[28]Murray M G,Thompson W F.Rapid isolation of high molecular weight plant DNA[J].Nucleic Acids Res,1980,8:4321-4326.
[29]Weng J F,Xie C X,Hao Z F,et al.Genome-wide association study identifies candidate genes that affect plant height in Chinese elite maize(Zea mays L.)inbred lines[J].PLo S One,2011,6:29229.
[30]Jesse A P,Peter J B,Edward S B,et al.Genome-wide nested association mapping of quantitative resistance to northern leaf blight in maize[J].PNAS,2011,108(17):6893-6898.
[31]Zhou J,Loh Y T,Bressan R A,et al.The tomato gene Pti1 encodes a serine/threonine kinase that is phosphorylated by Pto and is involved in the hypersensitive response[J].Cell,1995,83:925-935.
[32]Laplante A F,Moulin V,Auger F A,et al.Expression of heat shock proteins in mouse skin during wound healing[J].Journal of Histochemistry and Cytochemistry,1998,46(11):1291-1301.
[33]Jump up,De Maio A.Heat shock proteins:facts,thoughts,and dreams[J].Shock,1999,11(1):1-12.
[35]Ali F,Yan J B.The phenomenon of disease resistance in maize and the role of molecular breeding in defending against global threat[J].Integrated Plant Biol,2012,55:134-151.
[36]Horim L,Ok-Kyong C,Jen S.Stem-cell-triggered immunity through by CLV3p-FLS2 signaling[J].Nature,2011,473:376-379.