响应面试验优化超声波辅助提取龙眼愈伤组织中单宁的工艺
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摘要
为提高龙眼愈伤组织中单宁的提取率,采用超声波辅助浸提法,基于乙醇浓度(A)、超声波功率(B)与提取时间(C) 3个单因素试验,通过响应面试验优化设计,建立了龙眼愈伤组织单宁提取工艺。研究结果表明,龙眼愈伤组织中单宁的最佳提取条件为乙醇浓度61%、超声波功率140 W,提取时间54 min,提取温度60℃,在此工艺条件下,提取量为7.53 mg·g~(-1)。
In order to improve tannin extraction from longan callus,the effects of ethanol concentration,ultrasonic power and extraction time on the extraction of tannin were studied by ultrasound-assisted extraction.The tannin extraction procedure specification was then established using response surface methodology.The results show that optimized extraction of tannin(7.53 mg·g-1) could be obtained under the condition of ethanol concentration 61%,ultrasonic power 140 W,extraction time 54 min and extraction temperature 60 ℃.
In order to improve tannin extraction from longan callus,the effects of ethanol concentration,ultrasonic power and extraction time on the extraction of tannin were studied by ultrasound-assisted extraction.The tannin extraction procedure specification was then established using response surface methodology.The results show that optimized extraction of tannin(7.53 mg·g-1) could be obtained under the condition of ethanol concentration 61%,ultrasonic power 140 W,extraction time 54 min and extraction temperature 60 ℃.
引文
[1]吴妮妮.龙眼核的化学成分分析及龙眼核和龙眼壳的抗氧化活性测定[D].南宁:广西医科大学,2007.DOI:10.7666/d.y1138450WU N N.The Chemical Separation of Longan’s Seed and the Research of Longan Seed and Longan Hull’s Anti-oxidation[D].Nanning:Guangxi Medica University,2007.DOI:10.7666/d.y1138450
[2]RIPA F A,HAQUE M,BULBUL I J.In vitro antibacterial,cytotoxic and antioxidant activities of plant Nephelium longan[J].Pakistan Journal of Biological Sciences,2010,13(1):22-7.DOI:10.3923pjbs.2010.22.27
[3]黄占华,方桂珍.植物单宁的应用及研究进展[J].生物质化学工程,2005,39(5):39-43.DOI:10.3969/j.issn.1673-5854.2005.05.011HUANG Z H,FANG G Z.Application and research progresses of vegetable tannin[J].Journal of Chemical Industry of Forest Products,2005,39(5):39-43.DOI:10.3969/j.issn.1673-5854.2005.05.011
[4]于喆,金哲雄.植物单宁的应用及研究进展[J].黑龙江医药,2015(1):20-23.DOI:10.14035/j.cnki.hljyy.2015.01.007YU Z,JIN Z X.Research progress and application of vegetable Tannins[J].Heilongjiang Medicine Journal,2015(1):20-23.DOI:10.14035/j.cnki.hljyy.2015.01.007
[5]王磊,陈一宁,但年华,等.植物单宁的提取纯化方法及其发展[J].西部皮革,2015(12):12-19.DOI:10.3969/j.issn.1671-1602.2015.12.005WANG L,CHEN Y N,DAN N H,et al.The extraction and purification methods of vegetable tannin and their development[J].West Leather,2015(12):12-19.DOI:10.3969/j.issn.1671-1602.2015.12.005
[6]余先纯,李湘苏,龚铮午.石榴皮单宁的超声波-半仿生法提取及抗氧化性分析[J].化学研究与应用,2013,25(5):636-641.DOI:10.3969/j.issn.1004-1656.2013.05.005YU X C,LI X S,GONG Z W.Extraction of tannins from pomegranate peel with ultrasonic-assisted semibionic and antioxidation analysis[J].Chemical Research and Application,2013,25(5):636-641.DOI:10.3969/j.issn.1004-1656.2013.05.005
[7]刘细祥,张婷婷,兰翠玲,等.超声波提取香蕉皮中单宁工艺研究[J].食品工业,2014(2):22-25.LIU X X,ZHANG T T,LAN C L,et al.Study on tannins extraction from banana peel using ultrasonic[J].The Food Industry,2014(2):22-25.
[8]李倩云,张双灵,周国燕.超声波辅助提取五倍子中单宁的工艺优化[J].食品与机械,2015(1):171-174.LI Q Y,ZHANG S L,ZHOU G Y.Optimization on ultrasonic extraction technology of tannin from gallnut[J].Food and Machinery,2015(1):171-174.
[9]屈恋,高璇,刘雄民,等.柠檬桉果实单宁的提取纯化及抗氧化活性[J].食品工业科技,2017,38(8):256-261.DOI:10.13386/j.issn1002-0306.2017.08.041QU L,GAO X,LIU X M,et al.Study on purification and antioxidant activities of tannins of Eucalyptus citriodora fruits[J].Science and Technology of Food Industry,2017,38(8):256-261.DOI:10.13386/j.issn1002-0306.2017.08.041
[10]MINIERI S,ARIANNA B,STEFANO R,et al.Effect of quebracho tannin extract on soybean and linseed oil biohydrogenation by solid associated Bacteria:An in vitro study[J].Italian Journal of Animal Science,2014,13(3):3267.DOI:10.4081/ijas.2014.3267
[11]徐小萍,赖瑞联,林玉玲,等.真菌诱导子对龙眼胚性愈伤组织生长和多糖积累的影响[J].热带作物学报,2016,37(11):2216-2221.DOI:10.3969/j.issn.1000-2561.2016.11.029XU X P,LAI R L,LIN Y L,et al.Effects of fungal elicitors on growth and polysaccharide accumulation of embryogenic calli in Dimocarpus longan Lour[J]Chinese Journal of Tropical Crops,2016,37(11)2216-2221.DOI:10.3969/j.issn.1000-2561.2016.11.029
[12]赖钟雄,潘良镇,陈振光.龙眼胚性细胞系的建立与保持[J].福建农业大学学报,1997,26(2):160-167.LAI Z X,PAN L Z,CHEN Z G.Establishment and maintenance of longan embryogenic cell lines[J]Journal of Fujian Agricultural University,1997,26(2):160-167
[13]赖钟雄,陈春玲,黄素华,等.龙眼胚性愈伤组织长期继代培养及其染色体数目变异[J].福建农业大学学报,2001,30(1):29-32.DOI:10.3969/j.issn.1671-5470.2001.01.005LAI Z X,CHEN C L,HUANG S H,et al.Long-term subculture of embryogenic calli and their genetic variation of the chromosome number in longan[J].Journal of Fujian Agricultural University,2001,30(1):29-32DOI:10.3969/j.issn.1671-5470.2001.01.005
[14]YU X C,SUN D L,LI X S.Preparation of levulinic acid using cellulase and solid acid synergistic hydrolysis for rice straw[J].Asian Journal of Chemistry,2010,22(9):7113-7122.
[15]杨云.苜蓿属植物皂苷含量及其遗传变异的研究[D]兰州:甘肃农业大学,2010.DOI:10.7666/d y1739966YANG Y.Study on Saponin Content and Genetic Variation in Medicago[D].Lanzhou:Gansu Agriculture University,2010.DOI:10.7666/d.y1739966
[16]廖维良,赵美顺,杨红.超声波辅助提取技术研究进展[J].广东药学院学报,2012,28(3):347-350.DOI:10.3969/j.issn.1006-8783.2012.03.029LIAO W L,ZHAO M S,YANG H.Research progress on ultrasonic-assisted extraction technology[J].Journal of Guangdong Pharmaceutical University,2012,28(3):347-350.DOI:10.3969/j.issn.1006-8783.2012.03.029
[17]钟雨珅,王历琼,韩小丹,等.正交试验优化超声波辅助同时提取桑叶总黄酮、单宁工艺[J].食品科学,2015,36(12):44-48.DOI:10.7506/spkx1002-6630-201512008ZHONG Y K,WANG L Q,HAN X D,et al.Optimization of simultaneous ultrasonic-assisted extraction of total flavonoids and tannins from mulberry leaves[J].Food Science,2015,36(12):44-48.DOI:10.7506/spkx1002-6630-201512008
[18]魏学军,林先燕,冯光维,等.响应面法优化一贯煎中多糖的提取工艺[J].中国实验方剂学杂志,2011,17(9):22-25.DOI:10.3969/j.issn.1005-9903.2011.09.006WEI X J,LIN X Y,FENG G W,et al.Optimization of extraction craft of polysaccharides in Yiguanjian by response surface method[J].Chinese Journal of Experimental Traditional Medical Formulae,2011,17(9):22-25.DOI:10.3969/j.issn.1005-9903.2011.09.006
[19]王东艳.植物愈伤组织培养的影响因子[J].科技视界,2015(9):133-133.DOI:10.3969/j.issn.2095-2457.2015.09.101WANG D Y.The influence factor of plant callus culture[J].Science&Technology Vision,2015(9):133-133.DOI:10.3969/j.issn.2095-2457.2015.09.101
[2]RIPA F A,HAQUE M,BULBUL I J.In vitro antibacterial,cytotoxic and antioxidant activities of plant Nephelium longan[J].Pakistan Journal of Biological Sciences,2010,13(1):22-7.DOI:10.3923pjbs.2010.22.27
[3]黄占华,方桂珍.植物单宁的应用及研究进展[J].生物质化学工程,2005,39(5):39-43.DOI:10.3969/j.issn.1673-5854.2005.05.011HUANG Z H,FANG G Z.Application and research progresses of vegetable tannin[J].Journal of Chemical Industry of Forest Products,2005,39(5):39-43.DOI:10.3969/j.issn.1673-5854.2005.05.011
[4]于喆,金哲雄.植物单宁的应用及研究进展[J].黑龙江医药,2015(1):20-23.DOI:10.14035/j.cnki.hljyy.2015.01.007YU Z,JIN Z X.Research progress and application of vegetable Tannins[J].Heilongjiang Medicine Journal,2015(1):20-23.DOI:10.14035/j.cnki.hljyy.2015.01.007
[5]王磊,陈一宁,但年华,等.植物单宁的提取纯化方法及其发展[J].西部皮革,2015(12):12-19.DOI:10.3969/j.issn.1671-1602.2015.12.005WANG L,CHEN Y N,DAN N H,et al.The extraction and purification methods of vegetable tannin and their development[J].West Leather,2015(12):12-19.DOI:10.3969/j.issn.1671-1602.2015.12.005
[6]余先纯,李湘苏,龚铮午.石榴皮单宁的超声波-半仿生法提取及抗氧化性分析[J].化学研究与应用,2013,25(5):636-641.DOI:10.3969/j.issn.1004-1656.2013.05.005YU X C,LI X S,GONG Z W.Extraction of tannins from pomegranate peel with ultrasonic-assisted semibionic and antioxidation analysis[J].Chemical Research and Application,2013,25(5):636-641.DOI:10.3969/j.issn.1004-1656.2013.05.005
[7]刘细祥,张婷婷,兰翠玲,等.超声波提取香蕉皮中单宁工艺研究[J].食品工业,2014(2):22-25.LIU X X,ZHANG T T,LAN C L,et al.Study on tannins extraction from banana peel using ultrasonic[J].The Food Industry,2014(2):22-25.
[8]李倩云,张双灵,周国燕.超声波辅助提取五倍子中单宁的工艺优化[J].食品与机械,2015(1):171-174.LI Q Y,ZHANG S L,ZHOU G Y.Optimization on ultrasonic extraction technology of tannin from gallnut[J].Food and Machinery,2015(1):171-174.
[9]屈恋,高璇,刘雄民,等.柠檬桉果实单宁的提取纯化及抗氧化活性[J].食品工业科技,2017,38(8):256-261.DOI:10.13386/j.issn1002-0306.2017.08.041QU L,GAO X,LIU X M,et al.Study on purification and antioxidant activities of tannins of Eucalyptus citriodora fruits[J].Science and Technology of Food Industry,2017,38(8):256-261.DOI:10.13386/j.issn1002-0306.2017.08.041
[10]MINIERI S,ARIANNA B,STEFANO R,et al.Effect of quebracho tannin extract on soybean and linseed oil biohydrogenation by solid associated Bacteria:An in vitro study[J].Italian Journal of Animal Science,2014,13(3):3267.DOI:10.4081/ijas.2014.3267
[11]徐小萍,赖瑞联,林玉玲,等.真菌诱导子对龙眼胚性愈伤组织生长和多糖积累的影响[J].热带作物学报,2016,37(11):2216-2221.DOI:10.3969/j.issn.1000-2561.2016.11.029XU X P,LAI R L,LIN Y L,et al.Effects of fungal elicitors on growth and polysaccharide accumulation of embryogenic calli in Dimocarpus longan Lour[J]Chinese Journal of Tropical Crops,2016,37(11)2216-2221.DOI:10.3969/j.issn.1000-2561.2016.11.029
[12]赖钟雄,潘良镇,陈振光.龙眼胚性细胞系的建立与保持[J].福建农业大学学报,1997,26(2):160-167.LAI Z X,PAN L Z,CHEN Z G.Establishment and maintenance of longan embryogenic cell lines[J]Journal of Fujian Agricultural University,1997,26(2):160-167
[13]赖钟雄,陈春玲,黄素华,等.龙眼胚性愈伤组织长期继代培养及其染色体数目变异[J].福建农业大学学报,2001,30(1):29-32.DOI:10.3969/j.issn.1671-5470.2001.01.005LAI Z X,CHEN C L,HUANG S H,et al.Long-term subculture of embryogenic calli and their genetic variation of the chromosome number in longan[J].Journal of Fujian Agricultural University,2001,30(1):29-32DOI:10.3969/j.issn.1671-5470.2001.01.005
[14]YU X C,SUN D L,LI X S.Preparation of levulinic acid using cellulase and solid acid synergistic hydrolysis for rice straw[J].Asian Journal of Chemistry,2010,22(9):7113-7122.
[15]杨云.苜蓿属植物皂苷含量及其遗传变异的研究[D]兰州:甘肃农业大学,2010.DOI:10.7666/d y1739966YANG Y.Study on Saponin Content and Genetic Variation in Medicago[D].Lanzhou:Gansu Agriculture University,2010.DOI:10.7666/d.y1739966
[16]廖维良,赵美顺,杨红.超声波辅助提取技术研究进展[J].广东药学院学报,2012,28(3):347-350.DOI:10.3969/j.issn.1006-8783.2012.03.029LIAO W L,ZHAO M S,YANG H.Research progress on ultrasonic-assisted extraction technology[J].Journal of Guangdong Pharmaceutical University,2012,28(3):347-350.DOI:10.3969/j.issn.1006-8783.2012.03.029
[17]钟雨珅,王历琼,韩小丹,等.正交试验优化超声波辅助同时提取桑叶总黄酮、单宁工艺[J].食品科学,2015,36(12):44-48.DOI:10.7506/spkx1002-6630-201512008ZHONG Y K,WANG L Q,HAN X D,et al.Optimization of simultaneous ultrasonic-assisted extraction of total flavonoids and tannins from mulberry leaves[J].Food Science,2015,36(12):44-48.DOI:10.7506/spkx1002-6630-201512008
[18]魏学军,林先燕,冯光维,等.响应面法优化一贯煎中多糖的提取工艺[J].中国实验方剂学杂志,2011,17(9):22-25.DOI:10.3969/j.issn.1005-9903.2011.09.006WEI X J,LIN X Y,FENG G W,et al.Optimization of extraction craft of polysaccharides in Yiguanjian by response surface method[J].Chinese Journal of Experimental Traditional Medical Formulae,2011,17(9):22-25.DOI:10.3969/j.issn.1005-9903.2011.09.006
[19]王东艳.植物愈伤组织培养的影响因子[J].科技视界,2015(9):133-133.DOI:10.3969/j.issn.2095-2457.2015.09.101WANG D Y.The influence factor of plant callus culture[J].Science&Technology Vision,2015(9):133-133.DOI:10.3969/j.issn.2095-2457.2015.09.101