芸苔素内酯与苯甲丙环唑协同防控玉米小斑病初步研究
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摘要
为了研究芸苔素内酯与苯甲丙环唑共同作用防控玉米小斑病,利用生长速率法和孢子萌发法以及人工接种方法,测定芸苔素内酯对玉米小斑病菌的抑制作用,同时调查其防效并测定防御反应各项指标。结果显示,芸苔素内酯对玉米小斑病菌菌落及孢子均无明显抑制作用。施用芸苔素内酯和减量苯甲丙环唑混剂与单一施用常规用量苯甲丙环唑防效相近,防御反应结果基本一致,且发病前喷药效果更佳。因此本研究证实,利用芸苔素内酯可以减少苯甲丙环唑的用量且达到相同的防病效果,从而降低对环境的污染。
To study the prevention and control of Bipolaris maydis with brassinolide and chemical pesticides,the methods of growth rate,spore germination and artificial infection were used to determine the inhibition of B.maydis by spraying brassinolide and to investigate the control efficiency and the kinds of reaction.The results showed that there was no apparent inhibition of B.maydis after brassinolide was sprayed.It also showed that the results of control efficiency and level of defensive reaction have no obvious differences between the treatments of spraying brassinolide with decrement of Difenoconazole ·Propiconazole EC and only spraying Difenoconazole·Propiconazole EC.It also suggested that it would be better if the pesticide has been sprayed before invasion.Therefore,this study concluded that it would attain the same effect of disease prevention by using brassinolide to supply the decrement of Difenoconazole·Propiconazole EC in order to reduce the environment pollution.
To study the prevention and control of Bipolaris maydis with brassinolide and chemical pesticides,the methods of growth rate,spore germination and artificial infection were used to determine the inhibition of B.maydis by spraying brassinolide and to investigate the control efficiency and the kinds of reaction.The results showed that there was no apparent inhibition of B.maydis after brassinolide was sprayed.It also showed that the results of control efficiency and level of defensive reaction have no obvious differences between the treatments of spraying brassinolide with decrement of Difenoconazole ·Propiconazole EC and only spraying Difenoconazole·Propiconazole EC.It also suggested that it would be better if the pesticide has been sprayed before invasion.Therefore,this study concluded that it would attain the same effect of disease prevention by using brassinolide to supply the decrement of Difenoconazole·Propiconazole EC in order to reduce the environment pollution.
引文
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[16]李新杰,刘晓鹏.丙环唑的药害及其控制对策[J].中国植保导刊,2005,25(12):41-42.
[17]Liu J,Gao H,Wang X,et,al.Effects of 24-epibrassinolide on plant growth,osmotic regulation and ion homeostasis of salt-stressed canola[J].Plant Biology,2014,16(2):440-450.
[18]张玉江.冀东滨海稻区水稻主要病虫害防控技术[J].北方水稻,2011,41(6):48-50.
[19]Fu K,Liu L,Fan L,et al.Accumulation of copper in Trichodermaresei transformants,constructed with the modified Agrobacterium tumefaciens-mediated transformation technique[J].Biotechnology Letters,2010,32:1815-1820.
[2]王尽松,徐荣燕,李瑞花.30%苯甲·丙环唑EC对玉米后期叶斑病的防效[J].中国植保导刊,2013,33(1):49-50.
[3]王焕民.芸苔素内酯:植物生长发育的一种基本调节物质[J].农药,2000,39(1):11-14.
[4]伍小良.抗烟草花叶病毒病的药剂筛选及复配增效作用研究[D].重庆:西南大学,2007.
[5]李广领,陈锡岭,陈军,等.8种新型杀菌剂对玉米小斑病菌的室内毒力测定[A]//第六届全国农药交流会论文集[C].南京,2006.
[6]张婷,朱洁伟,武向文,等.拮抗木霉菌对玉米弯孢叶斑病的诱导抗性作用[J].上海交通大学学报(农业科学版),2011,29(4):38-41,60.
[7]房锋,纪春涛,聂乐兴,等.3种种衣剂对玉米种子保护作用及产量影响[J].农药科学与管理,2008,29:19-23.
[8]Lamb C J,Rubery P H.Phenylalanine ammonialyase and cinnamic and 4-hydroxylase:product repression of the level of enzyme activity in potato tuber discs[J].Planta,1976,130(3):283-290.
[9]郭红边,陈捷,高增贵,等.不同诱抗剂诱导玉米对灰斑病的抗性及其与PAL的关[J].沈阳农业大学学报,2000,31(5):465-467.
[10]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:164-165.
[11]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000:163-165.
[12]谢志霞.冠菌素对棉花苗期耐盐性的调控效应及机理[D].北京:中国农业大学,2007.
[13]陈秀,方朝阳.植物生长调节剂芸苔素内酯在农业上的应用现状及前景[J].世界农药,2015(2):34-36,42.
[14]龙友华,邱红波,何腾兵.四种植物生长调节剂浸种对玉米调控效应[J].中国农学通报,2011,27(9):106-110.
[15]伍小良,丁伟,刘荣华,等.新型植物生长调节剂对烟草花叶病的控制作用[J].中国农学通报,2007,23(4):307-310.
[16]李新杰,刘晓鹏.丙环唑的药害及其控制对策[J].中国植保导刊,2005,25(12):41-42.
[17]Liu J,Gao H,Wang X,et,al.Effects of 24-epibrassinolide on plant growth,osmotic regulation and ion homeostasis of salt-stressed canola[J].Plant Biology,2014,16(2):440-450.
[18]张玉江.冀东滨海稻区水稻主要病虫害防控技术[J].北方水稻,2011,41(6):48-50.
[19]Fu K,Liu L,Fan L,et al.Accumulation of copper in Trichodermaresei transformants,constructed with the modified Agrobacterium tumefaciens-mediated transformation technique[J].Biotechnology Letters,2010,32:1815-1820.