辅料姜汁对黄连生物碱成分的影响
|
摘要
目的考察采用辅料姜汁炮制前后黄连生物碱成分的变化。方法建立HPLC同时测定格兰地新、盐酸药根碱、非洲防己碱、表小檗碱、盐酸黄连碱、盐酸巴马汀、盐酸小檗碱7种成分含量的方法,并对辅料姜汁炮制前后黄连中生物碱成分含量进行测定和分析。结果方法学考察表明,测定方法准确度高、重复性好。经姜汁炮制后,黄连中格兰地新、盐酸药根碱和盐酸巴马汀含量显著提高(P<0.05),7种生物碱总含量有所提高。结论黄连经辅料姜汁炮制后,部分生物碱成分含量发生变化。
Objective To investigate the changes of alkaloids in Coptidis Rhizoma before and after processing with adjuvant ginger juice. Methods The HPLC method for determining the contents of granitin, jatrorrhizine hydrochloride, columbamine, epiberberine, coptisine hydrochloride, palmatine hydrochloride and berberine hydrochloride simultaneously was established. The contents of alkaloids from Coptidis Rhizoma were measured and analyzed before and after processing. Results The methodological study showed that the HPLC method was accurate and reproducible. The contents of granitin, jatrorrhizine hydrochloride and palmatine hydrochloride in Coptidis Rhizoma significantly increased by ginger juice processing(P<0.05). The total contents of seven alkaloids increased.Conclusion The contents of some alkaloids in Coptidis Rhizoma change after adjuvant ginger juice processing.
Objective To investigate the changes of alkaloids in Coptidis Rhizoma before and after processing with adjuvant ginger juice. Methods The HPLC method for determining the contents of granitin, jatrorrhizine hydrochloride, columbamine, epiberberine, coptisine hydrochloride, palmatine hydrochloride and berberine hydrochloride simultaneously was established. The contents of alkaloids from Coptidis Rhizoma were measured and analyzed before and after processing. Results The methodological study showed that the HPLC method was accurate and reproducible. The contents of granitin, jatrorrhizine hydrochloride and palmatine hydrochloride in Coptidis Rhizoma significantly increased by ginger juice processing(P<0.05). The total contents of seven alkaloids increased.Conclusion The contents of some alkaloids in Coptidis Rhizoma change after adjuvant ginger juice processing.
引文
[1]蒋泽述.浅谈药物姜制[J].时珍国医国药,1999,10(1):54-55.
[2]靳庆霞.浅谈药物姜制[J].中华中医药学刊,2006,24(9):1751.
[3]杨春雨,郭凤倩,臧琛,等.中药炮制用辅料姜汁的冻干工艺优化及冻干粉稳定性考察[J].中国中药杂志,2018,43(3):520-526.
[4]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:304.
[5]兰进,杨世林,郑玉权,等.黄连的研究进展[J].中草药,2001,32(12):1139-1141.
[6]童静玲,胡敏.不同炮制方法对黄连的的影响及其炮制品的辨证应用[J].海峡药学,2010,22(10):35.
[7]谢光远,杨金梅,龚千锋.黄连炮制品的研究进展[J].江西中医药,2009,,40(4):79.
[8]YANG C Y, GUO F Q, ZANG C, et al. The effect of ginger juice e processing on the chemical profiles of Rhizoma coptidis[J].Molecules,2018,23(2):380-394.
[9]WU H, WALDBAUER K,TANG L, et al.Influence of vinegar and wine processing on the alkaloid content and composition of the traditional Chinese medicine Corydalis Rhizoma(Yanhusuo)[J].Molecules,2014,19(8):11487-11504.
[10]舒华,向丽华.黄连药理作用及临床应用[J].甘肃中医医,2004,17(12):5-6.
[11]张瑞芬,苏和.黄连的药理研究进展[J].内蒙古中医药药,2010,29(3):114-117.
[2]靳庆霞.浅谈药物姜制[J].中华中医药学刊,2006,24(9):1751.
[3]杨春雨,郭凤倩,臧琛,等.中药炮制用辅料姜汁的冻干工艺优化及冻干粉稳定性考察[J].中国中药杂志,2018,43(3):520-526.
[4]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:304.
[5]兰进,杨世林,郑玉权,等.黄连的研究进展[J].中草药,2001,32(12):1139-1141.
[6]童静玲,胡敏.不同炮制方法对黄连的的影响及其炮制品的辨证应用[J].海峡药学,2010,22(10):35.
[7]谢光远,杨金梅,龚千锋.黄连炮制品的研究进展[J].江西中医药,2009,,40(4):79.
[8]YANG C Y, GUO F Q, ZANG C, et al. The effect of ginger juice e processing on the chemical profiles of Rhizoma coptidis[J].Molecules,2018,23(2):380-394.
[9]WU H, WALDBAUER K,TANG L, et al.Influence of vinegar and wine processing on the alkaloid content and composition of the traditional Chinese medicine Corydalis Rhizoma(Yanhusuo)[J].Molecules,2014,19(8):11487-11504.
[10]舒华,向丽华.黄连药理作用及临床应用[J].甘肃中医医,2004,17(12):5-6.
[11]张瑞芬,苏和.黄连的药理研究进展[J].内蒙古中医药药,2010,29(3):114-117.