玛咖对虚寒及虚热证大鼠环核苷酸及下丘脑-垂体-甲状腺轴水平的影响
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摘要
目的:研究玛咖对虚寒及虚热证大鼠环核苷酸及下丘脑-垂体-甲状腺轴水平的影响。方法:SD大鼠分为空白组、虚寒模型组、虚寒肉桂组(30g/kg)、虚寒玛咖高剂量组(2.4g/kg)、虚寒玛咖低剂量组(1.2g/kg)、虚热模型组、虚热黄柏组(5g/kg)、虚热玛咖高剂量组(2.4g/kg)、虚热玛咖低剂量组(1.2g/kg);采用每日分别肌肉注射氢化可的松琥珀酸钠(20mg/kg)、地塞米松磷酸钠注射液(0.35mg/kg)1次,连续21d的方法制备虚寒证、虚热证模型;通过放免法检测环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、促甲状腺激素释放激素(TRH)、促甲状腺激素(TSH)、三碘甲状腺原氨酸(T3)、甲状腺素(T4)、反三碘甲状腺原氨酸(rT3)含量变化。结果:与虚寒模型组比较,虚寒肉桂组及虚寒玛咖高、低剂量组cAMP、cAMP/cGMP、T3、T4均明显升高(P<0.05,P<0.01),cGMP、TRH、TSH、rT3均明显降低(P<0.05,P<0.01);而与虚热模型组比较,虚热玛咖高、低剂量组的cAMP、cGMP、cAMP/cGMP、TRH、TSH、T3、T4、rT3差异均无统计学意义。结论:玛咖对环核苷酸指标及下丘脑-垂体-甲状腺轴的调整与肉桂一致,与黄柏相反,通过同类比较,异类反证验证了玛咖温热药性。
Objective: To study the effects of maca on cyclic nucleotide and the hypothalamic-pituitary-thyroid axis in deficiency cold syndrome and deficiency heat syndrome rats. Methods: SD rats were divided into blank group, deficiency cold syndrome group, Cortex Cinnamomi deficiency cold syndrome(30 g/kg) group, maca deficiency cold syndrome high and low dose groups(2.4, 1.2 g/kg), deficiency heat syndrome group, Phellodendri Chinensis Cortex(PCC) deficiency heat syndrome group(5 g/kg), and maca deficiency heat syndrome high and low dose groups(2.4, 1.2 g/kg). The rats were treated with intramuscular injection of hydrocortisone(20 mg/kg) or dexamethasone sodium phosphate(0.35 mg/kg) for 21 days to establish the model of deficiency cold and deficiency heat syndromes. The change of cyclic adenosine monophosphate(cAMP), cyclic guanosine monophosphate(cGMP), thyroid stimulating hormone releasing hormone(TRH), thyroid stimulating hormone(TSH), triiodothyronine(T3), thyroxine(T4) and antitriiodothyronine(rT3) in each group were detected by radioimmunoassay. Results: The levels of cAMP, cAMP/cGMP, T3 and T4 increased and the levels of cGMP, TRH, TSH and rT3 decreased in Cortex Cinnamoni deficiency cold sydrome group and maca deficiency cold syndrome high and low dose groups(P<0.05, P<0.01). But no statistical significance was found on the levels of cAMP, cGMP, cAMP/cGMP, TRH, TSH, T3, T4 and rT3 in maca deficiency cold syndrome high and low dose groups. Conclusion: Maca showed same effect with Cinnamomi Cortex but opposite effect with PCC in adjusting hypothalamic-pituitary-thyroid axis, warm and heat properties of Maca were confirmed by similar comparison and opposite contrast.
Objective: To study the effects of maca on cyclic nucleotide and the hypothalamic-pituitary-thyroid axis in deficiency cold syndrome and deficiency heat syndrome rats. Methods: SD rats were divided into blank group, deficiency cold syndrome group, Cortex Cinnamomi deficiency cold syndrome(30 g/kg) group, maca deficiency cold syndrome high and low dose groups(2.4, 1.2 g/kg), deficiency heat syndrome group, Phellodendri Chinensis Cortex(PCC) deficiency heat syndrome group(5 g/kg), and maca deficiency heat syndrome high and low dose groups(2.4, 1.2 g/kg). The rats were treated with intramuscular injection of hydrocortisone(20 mg/kg) or dexamethasone sodium phosphate(0.35 mg/kg) for 21 days to establish the model of deficiency cold and deficiency heat syndromes. The change of cyclic adenosine monophosphate(cAMP), cyclic guanosine monophosphate(cGMP), thyroid stimulating hormone releasing hormone(TRH), thyroid stimulating hormone(TSH), triiodothyronine(T3), thyroxine(T4) and antitriiodothyronine(rT3) in each group were detected by radioimmunoassay. Results: The levels of cAMP, cAMP/cGMP, T3 and T4 increased and the levels of cGMP, TRH, TSH and rT3 decreased in Cortex Cinnamoni deficiency cold sydrome group and maca deficiency cold syndrome high and low dose groups(P<0.05, P<0.01). But no statistical significance was found on the levels of cAMP, cGMP, cAMP/cGMP, TRH, TSH, T3, T4 and rT3 in maca deficiency cold syndrome high and low dose groups. Conclusion: Maca showed same effect with Cinnamomi Cortex but opposite effect with PCC in adjusting hypothalamic-pituitary-thyroid axis, warm and heat properties of Maca were confirmed by similar comparison and opposite contrast.
引文
[1]牛建伟,彭勇,Gustavo F.Gonzales.健康新资源:玛卡.北京:中国医药科技出版社,2014:13-16
[2]Gonzales G F,Gonzales C,Gonzales-Castaneda C. Lepidiummeyenii(Maca):A plant from the highlands of Peru-from tradition to science.Forschende Komplementarmedizin/Research in Complementary Medicine,2009,16(6):373-380
[3]许利嘉,肖伟,马培,等.具开发前景的南美洲常用草药简介.现代药物与临床,2011,26(2):84-90
[4]谭晓蕾,彭勇.《本草纲目》收录外来药物的整理研究.中药材,2014,37(11):2099-2102
[5]肖小河,郭玉明,王伽伯,等.基于传统功效的中药寒热药性研究策论.世界科学技术-中医药现代化,2013,15(1):9-15
[6]张永忠,余龙江,万军梅,等.玛咖醇提物对正常小鼠免疫功能的影响.天然产物研究与开发,2007,19(2):274-276
[7]张洁宏,李彬,高玉秋,等.玛卡粉增强免疫力和抗疲劳功能动物实验研究.现代预防医学,2012,41(6):1096-1098
[8]胡远,李兴平,周毅,等.引种玛卡的抗疲劳实验研究.华西药学杂志,2015,30(4):440-441
[9]曹东,薛润光,顾鉴秋.丽江玛咖不同提取物对小鼠性行为及性激素的影响.云南中医中药杂志,2012,33(9):53-54
[10]Bo lin ZHENG,Kan H E,Calvin HYUNG,et al.Effect of a lipidic extract from Lepidiummeyenii on sexual behavior in mice and rats.Urology,2000,55(4):598-602
[11]Gustavo F Gonzales,Ana Ruiz,Carla Gonzales,et al.Effect of Lepidiummeyenii(maca)roots on spermatogenesis of male rats.Asian J Androl,2001,9(3):231-233
[12]张永忠,程华.玛咖提取物的激素样活性实验研究.时珍国医国药,2008,19(8):1874-1875
[13]彭璐,赵云,许旭东,等.玛咖对维甲酸诱导的骨质疏松症的抑制作用.中药药理与临床,2014,30(3):98-101
[14]周意,栾洁,刘玉香,等.玛咖多糖对果蝇的抗衰老作用.中国实验方剂学杂志,2014,20(18):151-154
[15]张永忠,余龙江,金文闻,等.玛咖多糖抗氧化保健作用研究.食品科技,2005,8(30):97-99
[16]余龙江,张永忠,金文闻,等.玛咖醇提取物对小鼠的抗衰老作用.中草药,2006,37(1):81-83
[17]Rostislav Vecera,Jan Orolin,Nina Skottova,et al.The influence of Maca(Lepidiummeyenii)on antioxidant status,lipid and glucose metabolism in rat.Plant Foods for Human Nutrition,2007,62:59-63
[18]张永忠,余龙江,敖明章,等.玛咖醇提取物对去卵巢大鼠内分泌激素及血脂水平的影响.中国新药杂志,2008,17(24):2112-2114,2121
[19]郑慧婷.玛咖提取物对氢溴酸东莨菪碱所致小鼠记忆获得障碍模型学习能力的影响.中医学报,2015,30(207):1170-1172
[20]Zhong Ai,Ai-Fang Cheng,Yuan-Tao Yu,et al.Antidepressantlike behavioral,anatomical,and biochemical effects of petroleum ether extract from Maca(Lepidiummeyenii)in mice exposed to chronic unpredictable mild stress.Journal of Medicinal Food,2014,17(5):535-542
[21]王竹,杨晶明,王国栋,等.玛咖独行菜对卵巢切除术后大鼠血脂及骨质代谢的影响.卫生研究,2009,38(4):420-422,425
[22]Stoner G D,Kresty L A,Carlton P S,et al.Isothiocyanates and freezedried strawberries as inhibitors of esophageal cancer.Toxicol Sci,1999,52(2):95-100
[23]王佳,张建军,费文婷,等.外来药物玛咖的英文数据库文献信息计量统计分析.中华中医药杂志,2018,33(3):1092-1096
[24]王淳,张建军,王佳,等.玛咖的临床文献研究及其中药性能探讨.中华中医药杂志,2017,32(12):5569-5573
[25]倪莉,张冰,刘小青,等.地塞米松磷酸钠诱导大鼠阴虚状态的实验研究.中华中医药杂志,2012,27(2):355
[26]刘欣,张冰,刘小青,等.辛热药和苦寒药对阳虚大鼠的不同药性生物学表达特征研究.中草药,2013,44(10):1295
[27]高学敏.中药学.北京:中国中医药出版社,2002:21-22
[28]孙广仁.中医基础理论.北京:中国中医药出版社,2007:43-45
[29]Wang Y,Xu A.Zheng:A systems biology approach to diagnosis and treatments.Science,2014,346(6216):13-15
[30]薛春苗,张冰,金锐,等.糖皮质激素诱导大鼠不同状态下CYP3A和GST活性变化研究.中国中医基础医学杂志,2011,17(11):1204-1206
[31]陈奇.c AMP、c GMP与中医阴阳学说.江西中医药,1980(2):12-17
[32]张翠珍,王天芳.c AMP、c GMP拮抗性代谢调节与中医证候关系研究进展.北京中医药大学学报,1999(6):51-53
[33]施雪筠.生理学.北京:中国中医药出版社,2007:253-258
[2]Gonzales G F,Gonzales C,Gonzales-Castaneda C. Lepidiummeyenii(Maca):A plant from the highlands of Peru-from tradition to science.Forschende Komplementarmedizin/Research in Complementary Medicine,2009,16(6):373-380
[3]许利嘉,肖伟,马培,等.具开发前景的南美洲常用草药简介.现代药物与临床,2011,26(2):84-90
[4]谭晓蕾,彭勇.《本草纲目》收录外来药物的整理研究.中药材,2014,37(11):2099-2102
[5]肖小河,郭玉明,王伽伯,等.基于传统功效的中药寒热药性研究策论.世界科学技术-中医药现代化,2013,15(1):9-15
[6]张永忠,余龙江,万军梅,等.玛咖醇提物对正常小鼠免疫功能的影响.天然产物研究与开发,2007,19(2):274-276
[7]张洁宏,李彬,高玉秋,等.玛卡粉增强免疫力和抗疲劳功能动物实验研究.现代预防医学,2012,41(6):1096-1098
[8]胡远,李兴平,周毅,等.引种玛卡的抗疲劳实验研究.华西药学杂志,2015,30(4):440-441
[9]曹东,薛润光,顾鉴秋.丽江玛咖不同提取物对小鼠性行为及性激素的影响.云南中医中药杂志,2012,33(9):53-54
[10]Bo lin ZHENG,Kan H E,Calvin HYUNG,et al.Effect of a lipidic extract from Lepidiummeyenii on sexual behavior in mice and rats.Urology,2000,55(4):598-602
[11]Gustavo F Gonzales,Ana Ruiz,Carla Gonzales,et al.Effect of Lepidiummeyenii(maca)roots on spermatogenesis of male rats.Asian J Androl,2001,9(3):231-233
[12]张永忠,程华.玛咖提取物的激素样活性实验研究.时珍国医国药,2008,19(8):1874-1875
[13]彭璐,赵云,许旭东,等.玛咖对维甲酸诱导的骨质疏松症的抑制作用.中药药理与临床,2014,30(3):98-101
[14]周意,栾洁,刘玉香,等.玛咖多糖对果蝇的抗衰老作用.中国实验方剂学杂志,2014,20(18):151-154
[15]张永忠,余龙江,金文闻,等.玛咖多糖抗氧化保健作用研究.食品科技,2005,8(30):97-99
[16]余龙江,张永忠,金文闻,等.玛咖醇提取物对小鼠的抗衰老作用.中草药,2006,37(1):81-83
[17]Rostislav Vecera,Jan Orolin,Nina Skottova,et al.The influence of Maca(Lepidiummeyenii)on antioxidant status,lipid and glucose metabolism in rat.Plant Foods for Human Nutrition,2007,62:59-63
[18]张永忠,余龙江,敖明章,等.玛咖醇提取物对去卵巢大鼠内分泌激素及血脂水平的影响.中国新药杂志,2008,17(24):2112-2114,2121
[19]郑慧婷.玛咖提取物对氢溴酸东莨菪碱所致小鼠记忆获得障碍模型学习能力的影响.中医学报,2015,30(207):1170-1172
[20]Zhong Ai,Ai-Fang Cheng,Yuan-Tao Yu,et al.Antidepressantlike behavioral,anatomical,and biochemical effects of petroleum ether extract from Maca(Lepidiummeyenii)in mice exposed to chronic unpredictable mild stress.Journal of Medicinal Food,2014,17(5):535-542
[21]王竹,杨晶明,王国栋,等.玛咖独行菜对卵巢切除术后大鼠血脂及骨质代谢的影响.卫生研究,2009,38(4):420-422,425
[22]Stoner G D,Kresty L A,Carlton P S,et al.Isothiocyanates and freezedried strawberries as inhibitors of esophageal cancer.Toxicol Sci,1999,52(2):95-100
[23]王佳,张建军,费文婷,等.外来药物玛咖的英文数据库文献信息计量统计分析.中华中医药杂志,2018,33(3):1092-1096
[24]王淳,张建军,王佳,等.玛咖的临床文献研究及其中药性能探讨.中华中医药杂志,2017,32(12):5569-5573
[25]倪莉,张冰,刘小青,等.地塞米松磷酸钠诱导大鼠阴虚状态的实验研究.中华中医药杂志,2012,27(2):355
[26]刘欣,张冰,刘小青,等.辛热药和苦寒药对阳虚大鼠的不同药性生物学表达特征研究.中草药,2013,44(10):1295
[27]高学敏.中药学.北京:中国中医药出版社,2002:21-22
[28]孙广仁.中医基础理论.北京:中国中医药出版社,2007:43-45
[29]Wang Y,Xu A.Zheng:A systems biology approach to diagnosis and treatments.Science,2014,346(6216):13-15
[30]薛春苗,张冰,金锐,等.糖皮质激素诱导大鼠不同状态下CYP3A和GST活性变化研究.中国中医基础医学杂志,2011,17(11):1204-1206
[31]陈奇.c AMP、c GMP与中医阴阳学说.江西中医药,1980(2):12-17
[32]张翠珍,王天芳.c AMP、c GMP拮抗性代谢调节与中医证候关系研究进展.北京中医药大学学报,1999(6):51-53
[33]施雪筠.生理学.北京:中国中医药出版社,2007:253-258