信号分子AI-2对长双歧杆菌BBMN68耐受胁迫能力的影响
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摘要
双歧杆菌是一种广泛应用的益生菌,但是受到环境胁迫后,菌株活力大幅降低,影响菌株功能发挥,其中信号分子AI-2在益生菌耐受环境胁迫中发挥关键作用。文章研究了外源添加AI-2对双歧杆菌BBMN68耐受酸、胆盐及氧胁迫能力的影响。结果表明,外源添加AI-2可以显著提高BBMN68在p H4.0酸胁迫的存活率(P<0.05),显著降低氧气的抑制作用(P<0.05),对在0.1%浓度胆盐下的生长无显著影响(P>0.05)。该研究表明,可以通过外源干预来提高长双歧杆菌的环境耐受能力。
Bifidobacterium is a widely used probiotics, but environmental stress can reduce strains viability and affect probiotic strain function. AI-2 plays a key role in probiotics' stress resistance. This study focused on the influence of AI-2 on acid, bile salt, oxygen tolerance resistance and biofilm formation of BBMN68. The results showed that after the addition of AI-2, the survival rate of BBMN68 significantly increased(P<0.05), and the inhibition of oxygen was significantly reduced(P<0.05). By contrast, the addition of AI-2 had no effect on 0.1% bile salt tolerance resistance(P>0.05). The results offer the possibility to improve probiotics' adversity tolerance by exogenous intervention and it lays the foundation on mechanism between LuxS/AI-2 QS and BBMN68' physiological property.
Bifidobacterium is a widely used probiotics, but environmental stress can reduce strains viability and affect probiotic strain function. AI-2 plays a key role in probiotics' stress resistance. This study focused on the influence of AI-2 on acid, bile salt, oxygen tolerance resistance and biofilm formation of BBMN68. The results showed that after the addition of AI-2, the survival rate of BBMN68 significantly increased(P<0.05), and the inhibition of oxygen was significantly reduced(P<0.05). By contrast, the addition of AI-2 had no effect on 0.1% bile salt tolerance resistance(P>0.05). The results offer the possibility to improve probiotics' adversity tolerance by exogenous intervention and it lays the foundation on mechanism between LuxS/AI-2 QS and BBMN68' physiological property.
引文
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[2]Tabbers M M,Milliano I D,Roseboom M G,et al.Is Bifidobacterium breve effective in the treatment of childhood constipation?Results from a pilot study[J].Nutrition Journal,2011,10(1):19.
[3]Zhang H J,Wang Y,Liu M F,et al.Effect of lipoteichoic acid of Bifidobacterium on senile phenotypes of aging mice induced by D-galactose[J].Chinese Journal of Microecology,2008.
[4]Simpson PJ,Ross RP,Fitzgerald GF,et al.Bifidobacterium psychraerophilum sp.nov.and Aeriscardovia aeriphila gen.nov,sp.nov,isolated from a porcine caecum[J].International Journal of Systematic and Evolutionary Microbiology,2004,54(2):401-406.
[5]Matsumoto M,Ohishi H,Benno Y.H+-ATPase activity in Bifidobacterium with special reference to acid tolerance[J].International journal of food microbiology,2004,93(1):109-113.
[6]Lacroix C,Yildirim S.Fermentation technologies for the production of probiotics with high viability and functionality[J].Current Opinion in Biotechnology,2007,18(2):176-183.
[7]Sun Z,He X,Brancaccio VF,et al.Bifidobacteria Exhibit LuxS-Dependent Autoinducer 2 Activity and Biofilm Formation[J].PLoS ONE,2014,9(2):e88260.
[8]Moslehi-Jenabian S,Gori K,Jespersen L.AI-2 signalling is induced by acidic shock in probiotic strains of Lactobacillus spp[J].International journal of food microbiology,2009,135(3):295-302.
[9]Wen Z T,Burne R A.LuxS-mediated signaling in Streptococcus mutans is involved in regulation of acid and oxidative stress tolerance and biofilm formation[J].Journal of Bacteriology,2004,186(9):2682-2691.
[10]Li L,Xu Z,Zhou Y,et al.Analysis on Actinobacillus pleuropneumoniae LuxS regulated genes reveals pleiotropic roles of LuxS/AI-2 on biofilm formation,adhesion ability and iron metabolism[J].Microbial Patho genesis,2011,50(6):293-302.
[11]Rickard AH,Palmer RJ,Blehert DS,et al.Autoinducer2:a concentration-dependent signal for mutualistic bacterial biofilm growth[J].Molecular microbiology,2006,60(6):1446-1456.
[12]Shao H,Lamont RJ,Demuth DR.Autoinducer 2 is required for Biofilm growth of Aggregatibacter(Actinobacillus)actinomycetemcomitans[J].Infection and Immunity,2007,75(9):4211-4218.
[13]Waters CM,Bassler BL.The Vibrio harveyi quorumsensing system uses shared regulatory components to discriminate between multiple autoinducers[J].Genes Dev,2006,20(19):2754-2767.
[14]Lebeer S,Verhoeven TL,Vélez MP,et al.Impact of environmental and genetic factors on biofilm formation by the probiotic strain Lactobacillus rhamnosus GG[J].Applied and environmental microbiology,2007,73(21):6768-6775.
[15]Riedel CU,Monk IR,Casey PG,et al.AgrD-dependent quorum sensing affects biofilm formation,invasion,virulence and global gene expression profiles in Listeria monocytogenes[J].Molecular microbiology,2009,71(5):1177-1189.