不同类型瓶装水中铜绿假单胞菌的数量变化研究
|
摘要
目的探究室温储存条件下,不同类型瓶装饮用水中铜绿假单胞菌的数量变化趋势。方法将计数好的铜绿假单胞菌菌悬液分别加入到纯净水、矿物质水、天然深层湖水、天然矿泉水等不同类型的瓶装饮用水中,跟踪检测保存不同天数后,水中的菌浓度变化情况。结果纯净水中铜绿假单胞菌的活菌数量在保存期间一直衰减,且菌的初始浓度低于2.5×10~5 CFU/250 mL时,在保存90 d后未检出活菌。矿物质水、天然深层湖水、天然矿泉水中,虽然铜绿假单胞菌的初始添加数量不一样,但均先出现一次明显的增长,然后再迅速下降,之后虽偶有小幅回升的情况,但总体呈衰减趋势;保存90d后,数量分别为50、300、2000CFU/mL。结论铜绿假单胞菌的数量在纯净水中直接衰减;在含有矿物元素的饮用水中,菌的数量呈现先增后减的趋势,并在3个月后依然保持较高的菌浓度,且活菌数量随着矿物元素的种类及浓度的增加而提升。
Objective To explore the change trend of Pseudomonas aeruginosa in different types of drinking water under room temperature storage. Methods The counted P. aeruginosa suspension was added to different types of drinking water, such as pure water, mineralized water, natural deep lake water and mineral water, then the change of bacteria concentration in water was detected continuously after different days. Results The number of P.aeruginosa in purified water declined during storage. When the initial concentration was less than 2.5×10~5 CFU/250 mL, no viable bacteria was detected after 90 days of storage. Although the initial amount of P. aeruginosa added in mineralized water, natural deep lake water and mineral water was different, there was a significant increase first and then a rapid falling. Despite there were occasional rebounds, it showed a general trend of attenuation. After 90 days of storage, the number of P. aeruginosa was 50, 300 and 2000 CFU/mL, respectively. Conclusion The number of P.aeruginosa declines directly in pure water. While the number of bacteria firstly increases and then decreases, and still remains a high concentration after 3 months in drinking water containing mineral elements. Furthermore, the number of living bacteria increases with the increase of types and concentrations of mineral elements.
Objective To explore the change trend of Pseudomonas aeruginosa in different types of drinking water under room temperature storage. Methods The counted P. aeruginosa suspension was added to different types of drinking water, such as pure water, mineralized water, natural deep lake water and mineral water, then the change of bacteria concentration in water was detected continuously after different days. Results The number of P.aeruginosa in purified water declined during storage. When the initial concentration was less than 2.5×10~5 CFU/250 mL, no viable bacteria was detected after 90 days of storage. Although the initial amount of P. aeruginosa added in mineralized water, natural deep lake water and mineral water was different, there was a significant increase first and then a rapid falling. Despite there were occasional rebounds, it showed a general trend of attenuation. After 90 days of storage, the number of P. aeruginosa was 50, 300 and 2000 CFU/mL, respectively. Conclusion The number of P.aeruginosa declines directly in pure water. While the number of bacteria firstly increases and then decreases, and still remains a high concentration after 3 months in drinking water containing mineral elements. Furthermore, the number of living bacteria increases with the increase of types and concentrations of mineral elements.
引文
[1]马群飞.瓶装饮用水铜绿假单胞菌污染研究进展[J].微生物学免疫学进展,2003,31(2):95-98.Ma QF.Research progress on contamination of bottled drinking water by Pseudomonas aeruginosa[J].Prog Microbiol Immunol,2003,31(2):95-98.
[2]邓军.包装饮用水中铜绿假单胞菌的检测方法[J].现代食品,2016,(1):104-105.Deng J.Detection method of Pseudomonas aeruginosa in packaged drinking water[J].Mod Food,2016,(1):104-105.
[3]包东东,牛贵洋.饮用水中铜绿假单胞菌的污染及控制[J].食品安全导刊,2016,(36):49.Bao DD,Niu GY.Pollution and control of Pseudomonas aeruginosa in drinking water[J].China Food Saf Magaz,2016,(36):49.
[4]徐述坚.桶装饮用水卫生质量状况[J].实用预防医学,2015,(22):124-126.Xu SJ.Analysis on the hygienic quality of bottled drinking water[J].Pract Prev Med,2015,(22):124-126.
[5]沈瑛,杨正林,岳凤.食物中毒标本中检出铜绿假单胞菌[J].浙江预防医学,2010,(22):45-46.Shen Y,Yang ZL,Yue F.Pseudomonas aeruginosa was detected in food poisoning samples[J].Zhejiang Prev Med,2010,(22):45-46.
[6]闫芳,隋英杰,孙静,等.桶装饮用水污染引起学生食物中毒的调查分析[J].中国卫生检验杂志,2011,(21):2082.Yan F,Sui YJ,Sun J,et al.Investigation and analysis of food poisoning caused by barreled drinking water pollution among students[J].Chin JHealth Lab Technol,2011,(21):2082.
[7]GB 19298-2014食品安全国家标准包装饮用水[S].GB 19298-2014 National food safety standard-Packaging of drinking water[S].
[8]钟菲菲.包装饮用水铜绿假单胞菌的污染与预防[J].现代食品,2017,(1):88-90.Zhong FF.Contamination and prevention of Pseudomonas aeruginosa in packaged drinking water[J].Mod Food,2017,(1):88-90.
[9]石文松,胡卓,罗雨阳,等.某市包装饮用水铜绿假单胞菌污染状况分析[J].食品安全质量检测学报,2018,9(4):757-760.Shi WS,Hu Z,Luo YY,et al.Analysis of the contamination status of Pseudomonas aeruginosa in packed drinking water in some city[J].JFood Saf Qual,2018,9(4):757-760.
[10]罗绍楠,戴奕杰,孙端方.贵州省包装饮用水中铜绿假单胞菌污染情况分析[J].食品安全导刊,2017,(8):72-73.Luo SN,Dai YJ,Sun DF.Survey of Pseudomonas aeruginosa contamination in packaged drinking water in Guizhou province[J].China Food Saf Magaz,2017,(8):72-73.
[11]GB 8538-2016食品安全国家标准饮用天然矿泉水检验方法[S].GB 8538-2016 National food safety standard-Test method for drinking natural mineral water[S].
[12]GB 4789-2016食品安全国家标准食品微生物学检验[S].GB 4789-2016 National food safety standard-Food microbiological examination[S].
[13]马群飞.瓶装饮用纯净水细菌数量变化规律研究[J].食品科学,2000,(9):52-54.Ma QF.The quantitative study of bacteria in bottled purified drinking water[J].Food Sci,2000,(9):52-54.
[14]Nicholas D.Technology of bottled water(3rd Edition)[M].Beijing:China Light Industry Press,2013.
[15]VanderKooij.Growth measurements with Pseudomonas aeruginosa,Aeromonas hydrophila and autochthonous bacteria to determine the biological stability of drinking water[J].Riv Ital Ig,1990,5(6):375-383.
[16]文刚.饮用水处理过程中细菌和生物稳定性变化规律的研究[D].哈尔滨:哈尔滨工业大学,2012.Wen G.Research on the variation of bacteria and biostability during drinking water treatment processes[D].Harbin:Harbin Institute of Technology,2012.
[17]郭素菊,童和平,张兴德.不同离子浓度的硫酸镁对铜绿假单胞菌生长的影响[J].陕西医学检验,1993,(1):33-34.Guo SJ,Tong HP,Zhang XD.Effects of magnesium sulfate with different ion concentrations on the growth of Pseudomonas aeruginosa[J].Shanxi JMed Lab Sci,1993,(1):33-34.
[2]邓军.包装饮用水中铜绿假单胞菌的检测方法[J].现代食品,2016,(1):104-105.Deng J.Detection method of Pseudomonas aeruginosa in packaged drinking water[J].Mod Food,2016,(1):104-105.
[3]包东东,牛贵洋.饮用水中铜绿假单胞菌的污染及控制[J].食品安全导刊,2016,(36):49.Bao DD,Niu GY.Pollution and control of Pseudomonas aeruginosa in drinking water[J].China Food Saf Magaz,2016,(36):49.
[4]徐述坚.桶装饮用水卫生质量状况[J].实用预防医学,2015,(22):124-126.Xu SJ.Analysis on the hygienic quality of bottled drinking water[J].Pract Prev Med,2015,(22):124-126.
[5]沈瑛,杨正林,岳凤.食物中毒标本中检出铜绿假单胞菌[J].浙江预防医学,2010,(22):45-46.Shen Y,Yang ZL,Yue F.Pseudomonas aeruginosa was detected in food poisoning samples[J].Zhejiang Prev Med,2010,(22):45-46.
[6]闫芳,隋英杰,孙静,等.桶装饮用水污染引起学生食物中毒的调查分析[J].中国卫生检验杂志,2011,(21):2082.Yan F,Sui YJ,Sun J,et al.Investigation and analysis of food poisoning caused by barreled drinking water pollution among students[J].Chin JHealth Lab Technol,2011,(21):2082.
[7]GB 19298-2014食品安全国家标准包装饮用水[S].GB 19298-2014 National food safety standard-Packaging of drinking water[S].
[8]钟菲菲.包装饮用水铜绿假单胞菌的污染与预防[J].现代食品,2017,(1):88-90.Zhong FF.Contamination and prevention of Pseudomonas aeruginosa in packaged drinking water[J].Mod Food,2017,(1):88-90.
[9]石文松,胡卓,罗雨阳,等.某市包装饮用水铜绿假单胞菌污染状况分析[J].食品安全质量检测学报,2018,9(4):757-760.Shi WS,Hu Z,Luo YY,et al.Analysis of the contamination status of Pseudomonas aeruginosa in packed drinking water in some city[J].JFood Saf Qual,2018,9(4):757-760.
[10]罗绍楠,戴奕杰,孙端方.贵州省包装饮用水中铜绿假单胞菌污染情况分析[J].食品安全导刊,2017,(8):72-73.Luo SN,Dai YJ,Sun DF.Survey of Pseudomonas aeruginosa contamination in packaged drinking water in Guizhou province[J].China Food Saf Magaz,2017,(8):72-73.
[11]GB 8538-2016食品安全国家标准饮用天然矿泉水检验方法[S].GB 8538-2016 National food safety standard-Test method for drinking natural mineral water[S].
[12]GB 4789-2016食品安全国家标准食品微生物学检验[S].GB 4789-2016 National food safety standard-Food microbiological examination[S].
[13]马群飞.瓶装饮用纯净水细菌数量变化规律研究[J].食品科学,2000,(9):52-54.Ma QF.The quantitative study of bacteria in bottled purified drinking water[J].Food Sci,2000,(9):52-54.
[14]Nicholas D.Technology of bottled water(3rd Edition)[M].Beijing:China Light Industry Press,2013.
[15]VanderKooij.Growth measurements with Pseudomonas aeruginosa,Aeromonas hydrophila and autochthonous bacteria to determine the biological stability of drinking water[J].Riv Ital Ig,1990,5(6):375-383.
[16]文刚.饮用水处理过程中细菌和生物稳定性变化规律的研究[D].哈尔滨:哈尔滨工业大学,2012.Wen G.Research on the variation of bacteria and biostability during drinking water treatment processes[D].Harbin:Harbin Institute of Technology,2012.
[17]郭素菊,童和平,张兴德.不同离子浓度的硫酸镁对铜绿假单胞菌生长的影响[J].陕西医学检验,1993,(1):33-34.Guo SJ,Tong HP,Zhang XD.Effects of magnesium sulfate with different ion concentrations on the growth of Pseudomonas aeruginosa[J].Shanxi JMed Lab Sci,1993,(1):33-34.