不同地区大曲中可培养细菌的分离与鉴定
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摘要
以3种来自不同地区大曲(四川、山东和辽宁)为研究对象,通过分离培养和16SrRNA鉴定的方法研究不同地区大曲中可培养细菌的种类。结果显示,四川大曲的密度为0.71 g/cm~3,含水量为9.89%,pH值为6.99,从中共分离出14株细菌,经进一步确认分别为Bacillus sp.、B. licheniformis、Staphylococcus saprophyticus和Pantoea sp.。山东大曲的密度为0.68 g/cm~3,含水量为10.00%,p H值为6.16,从中共分离出14株细菌,经进一步确认分别为B. wiedmannii、B. tequilensis、Enterococcus lactis和Pseudomonas sp.;辽宁大曲的密度为0.86 g/cm~3,含水量为11.65%,pH值为6.58,从中共分离出9株细菌,经进一步确认分别为Acinetobacter johnsonii、E. faecali和Enterobacter sp.。四川和山东产大曲中均发现多种芽孢杆菌,在辽宁产大曲中发现了其他两种大曲中未发现的多株Acinetobacter属细菌。
The cultivable bacteria in Daqu(Sichuan,Shandong and Liaoning)was investigated. The results showed that the density,water content and pH of Sichuan Daqu were 0.71 g/cm~3,9.89 % and 6.99 respectively.A total of 14 strains were funded in Sichuan Daqu and they were identified as Bacillus sp.,B. licheniformis,S.saprophyticus and Pantoea sp. The density,water content and pH of Shandong Daqu were 0.68 g/cm3,10.00 %and 6.16 respectively and Pseudomonas sp.,E. lactis,B. tequilensis and B. wiedmannii was funded in Shandong Daqu. The density,water content and pH of Liaoning Dauq were 0.86 g/cm3,11.65 % and 6.58 respectively and Acinetobacter johnsonii,Enterobacter sp. and E. faecalis were funded in Liaoning Daqu.Bacillus was a common genus in Daqu and some pathogenic bacteria(Acinetobacter)were found in Liaoning Daqu.
The cultivable bacteria in Daqu(Sichuan,Shandong and Liaoning)was investigated. The results showed that the density,water content and pH of Sichuan Daqu were 0.71 g/cm~3,9.89 % and 6.99 respectively.A total of 14 strains were funded in Sichuan Daqu and they were identified as Bacillus sp.,B. licheniformis,S.saprophyticus and Pantoea sp. The density,water content and pH of Shandong Daqu were 0.68 g/cm3,10.00 %and 6.16 respectively and Pseudomonas sp.,E. lactis,B. tequilensis and B. wiedmannii was funded in Shandong Daqu. The density,water content and pH of Liaoning Dauq were 0.86 g/cm3,11.65 % and 6.58 respectively and Acinetobacter johnsonii,Enterobacter sp. and E. faecalis were funded in Liaoning Daqu.Bacillus was a common genus in Daqu and some pathogenic bacteria(Acinetobacter)were found in Liaoning Daqu.
引文
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[2]宋瑞滨,邵泽良,宋军.浓香型大曲贮存过程中部分理化指标及微生物变化[J].酿酒科技,2017(1):59-61
[3]邢钢,敖宗华,王松涛,等.不同温度大曲制曲过程理化指标变化分析研究[J].酿酒科技,2014(6):20-23
[4]赵金松,张立强,郑佳,等.浓香大曲特征与工艺的相关性[J].食品与发酵工业,2013,39(12):50-55
[5]谭崇尧,徐军.高通量测序法对不同地域浓香型大曲微生物结构的分析[J].酿酒科技,2018(9):118-122
[6]朱文优,聂尧,徐岩.不同季节大曲生产过程中真菌群落结构的演变[J].食品与机械,2017,33(10):16-22
[7]李艳,董振玲,李佳,等.PCR-DGGE技术检测羊羔美酒大曲中细菌多样性[J].食品科学,2015,36(12):142-147
[8]李斌,闫志鹏,李慧星,等.基于高通量测序技术的浓香型和芝麻香型白酒酒曲细菌群落结构分析[J].中国酿造,2018,37(8):148-152
[9]罗莉,李欣,常煦,等.芽孢杆菌在中国白酒酿造中的应用机理研究进展[J].酿酒科技,2019(1):99-104
[10]张春林.泸州老窖大曲的质量、微生物与香气成分关系[D].无锡:江南大学,2012:76
[11]周新虎,陈翔,李燕荣,等.洋河大曲主要菌系的研究[J].酿酒科技,2011(9):56-59
[12]刘凡,仇钰莹,周新虎,等.洋河浓香型白酒酒醅中产酸细菌与有机酸合成的相关性研究[J].食品与发酵工业,2018,44(12):22-29
[13]姚霞,明红梅,周健,等.泸型大曲冬夏两季培菌期微生态的比较研究[J].食品研究与开发,2015,36(3):134-137
[14]李豆南,邱树毅.酱香大曲微生物菌群结构及微生物功能研究概述[J].中国酿造,2017,36(1):5-11
[15]刘洋,赵燃,黎妮,等.超级杂交水稻种子内生细菌群落结构及其多样性[J].食品与发酵工业,2016,42(1):31-36
[16]张一凡,李昌辉,李平兰,等.低盐发酵香肠用优良菌株的分离筛选及鉴定[J].肉类研究,2018,32(8):14-21
[17]窦晓,韩培杰,刘莉,等.泸型酒酿造期间酒醅中可培养细菌的分离鉴定及其种群演替规律研究[J].食品工业科技,2017,38(1):169-174
[18]刘晓玲,王金晶,李永仙,等.产β-1,3-1,4-葡聚糖酶特基拉芽孢杆菌Bacillus tequilensis CGX5-1发酵培养基的优化[J].食品与生物技术学报,2013,32(6):645-650
[19]高亦豹,王海燕,徐岩.利用PCR-DGGE未培养技术对中国白酒高温和中温大曲细菌群落结构的分析[J].微生物学通报,2010,37(7):999-1004
[20]曾丽云,袁玉菊,张倩颖,等.窖泥细菌群落结构与基酒挥发性组分相关性分析[J].食品科技,2017,42(2):9-13
[21]李维青.浓香型白酒与乳酸菌、乳酸、乳酸乙酯[J].酿酒,2010,37(3):90-93
[22]柴艳兵,岳田利,李兴佳,等.酸乳生产中微生物分布图谱的建立[J].食品科技,2015,40(11):2-8
[23]张明明.植物发酵食品中有害微生物安全分析[D].天津:天津科技大学,2016:20-24