连翘药材、标准汤剂、中间体和配方颗粒的HPLC指纹图谱相关性
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摘要
建立了连翘药材、标准汤剂、中间体和配方颗粒的HPLC指纹图谱,并对其相关性进行研究。采用Agilent Eclipse XDB-C_(18)反相色谱柱,以甲醇和水为流动相进行梯度洗脱,流速1.0 ml/min,柱温25℃,检测波长235 nm。连翘10批药材、标准汤剂、中间体及配方颗粒指纹图谱均呈现10个共有特征峰,呈良好的相关性。在10个共有峰中分析确认了6个化学成分,分别为forsythenside F(3号峰)、连翘酯苷Ⅰ(4号峰)、(+)-松脂醇-4-O-β-D-吡喃葡萄糖苷(5号峰)、连翘酯苷A(7号峰)、连翘苷(8号峰)及连翘脂素(10号峰)。连翘药材、标准汤剂、中间体和配方颗粒的主要化学成分组成基本相同,建立的HPLC指纹图谱方法可用于连翘配方颗粒生产全过程的质量控制。
In this study, the HPLC fingerprint spectra of the raw herbs, standard decoction, intermediates and dispensing granules of Forsythia suspense were established. The correlations among them were analyzed. An Agilent Eclipse XDB-C_(18) RP column was used with methanol and water as the mobile phase with a linear gradient elution. The flow rate was 1.0 ml/min, the column temperature was 25 ℃ with the detection wavelength of 235 nm. There were ten common characteristic peaks in the fingerprint spectra of the ten batches of raw herbs, standard decoction, intermediates and dispensing granules of Forsythia suspense. The results presented a good correlation. Six peaks were identified from the ten common peaks, including forsythenside F(peak 3), forsythoside Ⅰ(peak 4),(+)-pinoresinol-4-O-β-Dglucopyranoside(peak 5), forsythoside A(peak 7), forsythin(peak 8) and phillygenin(peak 10). The main chemical compositions existing in the raw herbs, standard decoction, intermediates and dispensing granules of Forsythia suspenseare basically the same. The established HPLC fingerprints can be used to control the quality in the whole production process of Forsythia suspense dispensing granules.
In this study, the HPLC fingerprint spectra of the raw herbs, standard decoction, intermediates and dispensing granules of Forsythia suspense were established. The correlations among them were analyzed. An Agilent Eclipse XDB-C_(18) RP column was used with methanol and water as the mobile phase with a linear gradient elution. The flow rate was 1.0 ml/min, the column temperature was 25 ℃ with the detection wavelength of 235 nm. There were ten common characteristic peaks in the fingerprint spectra of the ten batches of raw herbs, standard decoction, intermediates and dispensing granules of Forsythia suspense. The results presented a good correlation. Six peaks were identified from the ten common peaks, including forsythenside F(peak 3), forsythoside Ⅰ(peak 4),(+)-pinoresinol-4-O-β-Dglucopyranoside(peak 5), forsythoside A(peak 7), forsythin(peak 8) and phillygenin(peak 10). The main chemical compositions existing in the raw herbs, standard decoction, intermediates and dispensing granules of Forsythia suspenseare basically the same. The established HPLC fingerprints can be used to control the quality in the whole production process of Forsythia suspense dispensing granules.
引文
[1]胡静,马琳,张坚,等.连翘的研究进展[J].中南药学,2012,10(10):760-764.
[2] LI H B, CHEN F. Preparative isolation and purification of phillyrin from the medicinal plant Forsythia suspensa by highspeed counter-current chromatograph[J]. J Chromatogr A,2005, 1083(1/2):102-105.
[3]白雁,段小彦,雷敬卫,等.近红外漫反射光谱法在青翘含量测定中的应用[J].中国实验方剂学杂志,2013,19(23):111-114.
[4]范毅,陈玲,朱杰,等.连翘叶化学成分[J].中国实验方剂学杂志,2015, 21(24):22-25.
[5]曹静亚,李晓,宋梦娇,等.连翘标准汤剂质量评价体系的建立[J].中国实验方剂学杂志,2018, 24(7):7-12.
[2] LI H B, CHEN F. Preparative isolation and purification of phillyrin from the medicinal plant Forsythia suspensa by highspeed counter-current chromatograph[J]. J Chromatogr A,2005, 1083(1/2):102-105.
[3]白雁,段小彦,雷敬卫,等.近红外漫反射光谱法在青翘含量测定中的应用[J].中国实验方剂学杂志,2013,19(23):111-114.
[4]范毅,陈玲,朱杰,等.连翘叶化学成分[J].中国实验方剂学杂志,2015, 21(24):22-25.
[5]曹静亚,李晓,宋梦娇,等.连翘标准汤剂质量评价体系的建立[J].中国实验方剂学杂志,2018, 24(7):7-12.