白沙绿茶提取物对原发性高血压大鼠降压效果的研究
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摘要
目的:探讨白沙绿茶提取物对原发性高血压大鼠(SHR)的降压效果及其机制。方法:将60只原发性高血压大鼠(SHR)随机分为高血压对照组,苯磺酸氨氯地平组和白沙绿茶提取物高、中、低剂量组,共5组,每组12只。每日给予相应药物灌胃,对照组给予等量蒸馏水。每周对各组大鼠血压测量1次;7周末,自腹主动脉取血,用于测定血清一氧化氮(NO)、超氧化物歧化酶(SOD)活性以及血管紧张素Ⅱ(AngⅡ)含量,同时取心脏组织HE染色后观察病理组织形态学的变化。结果:与高血压对照组相比,白沙绿茶提取物三个剂量组与氨氯地平组均对大鼠的血压有一定的降低趋势,差异具有统计学意义(P<0.01),白沙绿茶提取物高(200 mg·kg~(-1)·d~(-1))、中(100 mg·kg~(-1)·d~(-1))剂量组和氨氯地平组(0.5 mg·kg~(-1)·d~(-1))可显著升高血清NO和SOD含量,差异均具有统计学意义(P<0.05或P<0.01)。结论:白沙绿茶提取物可升高SHR大鼠血清NO和SOD水平以及降低血管紧张素Ⅱ(AngⅡ)含量,产生降压效应,同时能有效逆转高血压大鼠病理性心肌重构。
Objective: To explore the effect of Baisha greentea extract on blood pressure in treating spontaneous hypertension rats. Methods: A total of 60 primary hypertensive rats( SHR) were randomly divided into hypertension of control group,amlodipine besylate group and the high,medium and low dose group of green tea extract of five groups,with twelve rats in each group. All treatments were given through oral,and the control group with distilled water. Each measurement of rat blood pressure was detected in themorning every week. After 7 weeks treatment,blood was collected from the rat abdominal aorta for determination of serum nitric oxide( NO) and superoxide dismutase( SOD) activity and level of plasma angiotensin II. At the same time,heart tissue was embedded in10% neutral buffered formaldehyde fixation,paraffin and sectioned. Then the changes of histopathological were changed after HE staining. Results: Compared with the SHR control group,systolic and diastolic blood pressure of essential hypertension in high dose group and middle dose group and amlodipine group was significantly reduced. At the same time,the NO and SOD contents of Baisha greentea extract in high dose group( 200 mg·kg~(-1)·d~(-1)) and middle dose group( 100 mg·kg~(-1)·d~(-1)) and amlodipine group( 0. 5 mg·kg~(-1)·d~(-1)) was significantly increased( P < 0. 05 and P < 0. 01). Conclusion: Long-term ingestion of Baisha greentea extract could significantly elevated serum NO and SOD levels of SHR,reduce the level of plasma angiotensin Ⅱ to common level,while reverse the hypertensive rat myocardial remodeling effectively.
Objective: To explore the effect of Baisha greentea extract on blood pressure in treating spontaneous hypertension rats. Methods: A total of 60 primary hypertensive rats( SHR) were randomly divided into hypertension of control group,amlodipine besylate group and the high,medium and low dose group of green tea extract of five groups,with twelve rats in each group. All treatments were given through oral,and the control group with distilled water. Each measurement of rat blood pressure was detected in themorning every week. After 7 weeks treatment,blood was collected from the rat abdominal aorta for determination of serum nitric oxide( NO) and superoxide dismutase( SOD) activity and level of plasma angiotensin II. At the same time,heart tissue was embedded in10% neutral buffered formaldehyde fixation,paraffin and sectioned. Then the changes of histopathological were changed after HE staining. Results: Compared with the SHR control group,systolic and diastolic blood pressure of essential hypertension in high dose group and middle dose group and amlodipine group was significantly reduced. At the same time,the NO and SOD contents of Baisha greentea extract in high dose group( 200 mg·kg~(-1)·d~(-1)) and middle dose group( 100 mg·kg~(-1)·d~(-1)) and amlodipine group( 0. 5 mg·kg~(-1)·d~(-1)) was significantly increased( P < 0. 05 and P < 0. 01). Conclusion: Long-term ingestion of Baisha greentea extract could significantly elevated serum NO and SOD levels of SHR,reduce the level of plasma angiotensin Ⅱ to common level,while reverse the hypertensive rat myocardial remodeling effectively.
引文
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14 Dornas W C,Silva M,Tavares R,et al.Efficacy of the superoxide dismutase mimetic tempol in animal hypertension models:a meta-analysis[J].Journal of hypertension,2015,33(1):14-23.
15 Xiao L,Kirabo A,Wu J,et al.Renal Denervation Prevents Immune Cell Activation and Renal Inflammation in Angiotensin II-Induced Hypertension[J].Circulation research,2015,117(6):547-557.
16 Kuwabara Y,Horie T,Baba O,et al.MicroRNA-451 exacerbates lipotoxicity in cardiac myocytes and high-fat diet-induced cardiac hypertrophy in mice through suppression of the LKB1/AMPK pathway[J].Circulation research,2015,116(2):279-288.
17贺巧云.替米沙坦对原发性高血压心肌肥厚及左室舒张功能的影响[J].中国现代医生,2015,53(2):82-84.
2江宏.自拟降压茶治疗轻中度高血压病156例临床观察[J].特别健康(下),2014,3:370-370.
3何国兴.降压护心菊花茶[J].家庭医学(上半月),2014,9:53-53.
4佘颜,张国明,蔺晓源,等.苦丁茶对动脉粥样硬化大鼠血管内皮功能的影响[J].中国中医急症,2014,23(2):220-222.
5李军,辛勤,杨雁.绿茶多酚药理作用的研究进展[J].中国热带医学,2014,14(1):128-130.
6尹睿卓,王嘉仡,王帅,等.山绿茶研究进展[J].辽宁中医药大学学报,2015,17(12):81-84.
7魏伟,吴希美,李元建.药理实验方法学[M].第4版.北京:人民卫生出版社,2010.69.
8潘畅,徐峰,袁秋环,等.乙醛脱氢酶2与心脑血管疾病研究进展[J].中华心血管病杂志,2016,44(2):175-178.
9陈伟伟,隋辉,马丽媛.中国心脑血管病流行现况及防治进展[J].心脑血管病防治,2016,2:001.
10 Kato T,Mizuguchi N,Ito A.Blood pressure,renal biochemical parameters and histopathology in an original rat model of essential hypertension(SHRSP/Kpostrain)[J].Biomedical Research,2015,36(3):169-177.
11 Gao T,Zhu Z Y,Zhou X,et al.Chrysanthemum morifolium extract improves hypertension-induced cardiac hypertrophy in rats by reduction of blood pressure and inhibition of myocardial hypoxia inducible factor-1alpha expression[J].Pharmaceutical biology,2016,23:1-6.
12 Perros F,Ranchoux B,Izikki M,et al.Nebivolol for improving endothelial dysfunction,pulmonary vascular remodeling,and right heart function in pulmonary hypertension[J].Journal of the American College of Cardiology,2015,65(7):668-680.
13 Ghosh S,Gupta M,Xu W,et al.Phosphorylation inactivation of endothelial nitric oxide synthesis in pulmonary arterial hypertension[J].American Journal of Physiology-Lung Cellular and Molecular Physiology,2016,310(11):L1199-L1205.
14 Dornas W C,Silva M,Tavares R,et al.Efficacy of the superoxide dismutase mimetic tempol in animal hypertension models:a meta-analysis[J].Journal of hypertension,2015,33(1):14-23.
15 Xiao L,Kirabo A,Wu J,et al.Renal Denervation Prevents Immune Cell Activation and Renal Inflammation in Angiotensin II-Induced Hypertension[J].Circulation research,2015,117(6):547-557.
16 Kuwabara Y,Horie T,Baba O,et al.MicroRNA-451 exacerbates lipotoxicity in cardiac myocytes and high-fat diet-induced cardiac hypertrophy in mice through suppression of the LKB1/AMPK pathway[J].Circulation research,2015,116(2):279-288.
17贺巧云.替米沙坦对原发性高血压心肌肥厚及左室舒张功能的影响[J].中国现代医生,2015,53(2):82-84.