MCP-1及其基因多态性与2型糖尿病周围神经病变的相关性研究
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摘要
目的探讨单核细胞趋化蛋白-1(MCP-1)及其基因多态性与2型糖尿病合并糖尿病周围神经病变(DPN的相关性。方法收集187例2型糖尿病患者。根据是否伴发周围神经病变分为DPN组和T2D组,另选择同期体检的健康人群为HC组。采用聚合酶链反应-限制性酶切片段长度多态性分析技术检测MCP-1(-2518A/G)的基因多态性,酶联免疫吸附法测定血清MCP-1水平。结果 T2D组AA基因型频率显著高于HC组(<0.05);DPN组AA基因型频率和A等位基因频率显著高于HC组(<0.05)。T2D组和DPN组血清中MCP-1水平显著高于HC组(<0.05),DPN组MCP-1水平高于T2D组(<0.05)。T2D组AA基因型的MCP-1水平低于AG基因型及GG基因型(均<0.05)。DPN组AA基因型的MCP-1水平低于GG基因型(<0.05)。MCP-1水平、糖尿病病程、HbA1c与DPN的发生显著相关(均<0.05)。结论血清MCP-1水平与T2DM及DPN的发生相关;MCP-1(-2518A/G)的基因多态性与T2DM相关,但与DPN的发生无相关性。
引文
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[2]Edwards JL,Vincent AM,Cheng HT,et al.Diabetic neuropathy:mechanisms to management[J].Pharmacol Ther,2008,120(1):1-34.
[3]Vincent AM,Russell JW,Low P,et al.Oxidative stress in the pathogenesis of diabetic neuropathy[J].Endocr Rev,2004,25(4):612-628.
[4]Yagihashi S,Mizukami H,Sugimoto K.Mechanism of diabetic neuropathy:Where are we now and where to go[J]?J Diabetes Investig,2011,2(1):18-32.
[5]Melgarejo E,Medina MA,Sanchez-Jimenez F,et al.Monocyte chemoattractant protein-1:a key mediator in inflammatory processes[J].Int J Biochem Cell Biol,2009,41(5):998-1001.
[6]Jing Y,Zhu D,Bi Y,et al.Monocyte chemoattractant protein 1-2518 A/G polymorphism and susceptibility to type 2diabetes in a Chinese population[J].Clin Chim Acta,2011,412(5-6):466-469.
[7]Moon JY,Jeong L,Lee S,et al.Association of polymorphisms in monocyte chemoattractant protein-1 promoter with diabetic kidney failure in Korean patients with type 2 diabetes mellitus[J].J Korean Med Sci,2007,22(5):810-814.
[8]中华医学会糖尿病学分会.中国2型糖尿病防治指南(2013年版)[J].中华内分泌代谢杂志,2014,30(10):893-942.
[9]Pasnoor M,Dimachkie MM,Kluding P,et al.Diabetic neuropathy part 1:overview and symmetric phenotypes[J].Neurol Clin,2013,31(2):425-445.
[10]White FA,Sun J,Waters SM,et al.Excitatory monocyte chemoattractant protein-1signaling is up-regulated in sensory neurons after chronic compression of the dorsal root ganglion[J].Proc Natl Acad Sci US A,2005,102(39):14092-14097.
[11]Abbadie C,Lindia JA,Cumiskey AM,et al.Impaired neuropathic pain responses in mice lacking the chemokine receptor CCR2[J].Proc Natl Acad Sci USA,2003,100(13):7947-7952.
[12]马芙蓉,吉尚戎,陈慧,等.血清MCP-1水平及其基因多态性与2型糖尿病周围神经病变的相关性[J].兰州大学学报,2017,43(5):13-17.
[13]马江波,许鑫,马改改,等.单核细胞趋化蛋白-1基因多态性与浙江局部地区汉族人群2型糖尿病的相关性研究[J].浙江医学,2016,38(20):1646-1649.
[14]Tan X,Gao J,Shi Z,et al.MG132 induces expression of monocyte chemotactic protein-induced protein 1 in vascular smooth muscle cells[J].J Cell Physiol,2017,232(1):122-128.
[15]Gao YJ,Zhang L,Samad OA,et al.JNK-induced MCP-1 production in spinal cord astrocytes contributes to central sensitization and neuropathic pain[J].J Neurosci,2009,29(13):4096-4108.