基于代谢组学技术研究党参干预小鼠血清代谢物规律
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摘要
目的基于代谢组学技术研究党参对正常小鼠血清内源性代谢产物的影响。方法采用基于代谢组学技术氢核磁共振谱(1H-NMR)结合多元统计分析及单变量分析手段,比较党参干预小鼠与正常小鼠的血清内源性代谢物的变化。结果给予党参后小鼠机体内源性代谢物发生了明显变化。党参干预后小鼠血清与空白对照组比较有8种代谢物含量发生变化,胆碱、肌酸、谷氨酸、甘氨酸、谷氨酰胺含量升高,乳酸、磷酰胆碱、甘油磷酰胆碱含量下降。结论党参对正常小鼠机体代谢的影响可能与其改变内源性代谢物的能量代谢、氨基酸代谢等途径相关,为党参机制研究提供参考依据。
Objective To study the effects of Radix Codonopsis on serum endogenous metabolites in normal mice based on metabolomics. Methods Metabolomic techniques(1 H-NMR) added with multivariate and univariate analyses was used to investigate the changes in serum endogenous metabolites in mice given Radix Codonopsis compared with blank controls. Results Intervention with Radix Codonopsis resulted in remarkable alterations in serum endogenous metabolites in mice. Compared with the blank controls, mice given Radix Codonopsis presented significant changes in eight serum metabolites, among which, the serum levels of choline, creatine, glutamic acid, glycine and glutamine were increased, while those of lactic acid, phosphorylcholine and glycerylphosphorylcholine were lowered.Conclusion The effect of Radix Codonopsis on the metabolism in normal mice may be related to its actions on energy and amino acid metabolisms of mice endogenous metabolites. This study provides evidence for further mechanistic study on Radix Codonopsis.
Objective To study the effects of Radix Codonopsis on serum endogenous metabolites in normal mice based on metabolomics. Methods Metabolomic techniques(1 H-NMR) added with multivariate and univariate analyses was used to investigate the changes in serum endogenous metabolites in mice given Radix Codonopsis compared with blank controls. Results Intervention with Radix Codonopsis resulted in remarkable alterations in serum endogenous metabolites in mice. Compared with the blank controls, mice given Radix Codonopsis presented significant changes in eight serum metabolites, among which, the serum levels of choline, creatine, glutamic acid, glycine and glutamine were increased, while those of lactic acid, phosphorylcholine and glycerylphosphorylcholine were lowered.Conclusion The effect of Radix Codonopsis on the metabolism in normal mice may be related to its actions on energy and amino acid metabolisms of mice endogenous metabolites. This study provides evidence for further mechanistic study on Radix Codonopsis.
引文
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[15] Zeilhofer HU,Acuna MA,Gingras J,et al. Glycine receptors and glycine transporters:targets for novel analgesics[J]. Cell Mol Life Sci,2018,75(Suppl):1-19.
[2]栗征.国家卫生健康委拟增补党参等为食药物质[J].中医药导报,2018,24(11):127-127.
[3]孙政华,邵晶,郭玫.党参化学成分及药理作用研究进展[J].安徽农业科学,2015,43(33):174-176.
[4]樊长征,洪巧瑜.党参对人体各系统作用的现代药理研究进展[J].中国医药导报,2016,13(10):39-43.
[5]张志新,高晓燕.药物代谢组学研究现状[J].中国中药杂志,2018,43(6):1093-1098.
[6]四君子汤加味颗粒剂的研制和初步药效学评价[D].雅安:四川农业大学,2014.
[7] Dinges MM,Lytle C,Larive CK.1H NMR-Based identification of intestinally absorbed metabolites by ussing chamber analysis of the rat cecum[J]. Analyt Chem,2018,90(6):4196-4202.
[8] Morelli MAC,Iuliano A,Schettini SCA,et al. NMR metabolomics study of follicular fluid in women with cancer resorting to fertility preservation[J]. J Assis Reprod Genet,2018,35(11):2063-2070.
[9]陈曦,杨永霞,王淑美,等.基于NMR代谢组学研究脑脉通复方提取物对大鼠脑缺血再灌注模型的影响[J].中草药,2012,43(1):97-102.
[10]薛晓利,张建琴,马雯,等.基于1H-NMR代谢组学的黄芪甲苷对秀丽隐杆线虫寿命的延长作用研究[J].中草药,2018,49(10):59-66.
[11]李俊怡.胆碱对团头鲂脂肪代谢的调控机制研究[D].南京:南京农业大学,2016.
[12]刘莉,叶鹏.青少年甘油磷酸胆碱代谢和心血管病的危险因素:一项队列研究[J].中华高血压杂志,2016,24(10):999.
[13]马俊杰,王小龙,刘会平.不同证型非小细胞肺癌患者肿瘤组织代谢组学研究[J].中国中西医结合杂志,2015,35(6):659-663.
[14]杨则宜.肌酸的补充与运动能力[J].体育科学,2000,20(1):76-78.
[15] Zeilhofer HU,Acuna MA,Gingras J,et al. Glycine receptors and glycine transporters:targets for novel analgesics[J]. Cell Mol Life Sci,2018,75(Suppl):1-19.