茶叶活性成分的抗炎作用及其机制研究进展
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摘要
茶叶中主要活性成分如茶多酚、茶氨酸、茶黄素具有较好的抗炎作用,且茶多酚效果最佳。虽然茶多酚的抗氧化作用被世界公认,但这并不是其发挥抗炎作用的唯一机制,其机制应与抑制炎症因子、调节相关炎症信号通路、抗氧化等综合作用有关。本文综述了茶叶及茶叶成分茶多酚、茶氨酸、茶黄素的抗炎作用及其机制,其中重点阐述了主要儿茶素组分表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)的抗炎机制,为茶叶抗炎作用的深入研究提供参考,为健康饮茶和茶叶抗炎功能产品的研发提供理论依据。
The main active components in tea, such as tea polyphenols, theanine and theaflavins, have good anti-inflammatory effects, and tea polyphenols have the best effect. However, antioxidant properties may not be the sole mechanism of anti-inflammatory. The mechanisms of anti-inflammatory are related with the comprehensive work of inhibiting inflammatory factor, modulate inflammatory signaling pathways, antioxidant, etc. This paper reviewed the anti-inflammatory effects of the main active components in tea and its mechanisms, and mainly emphasized the anti-inflammatory mechanism of catechin component epigallocatechin gallate(EGCG). It provided references for further study on the anti-inflammatory effects of tea, and a theoretical basis for the research and development of healthy tea drinking and tea anti-inflammatory products.
The main active components in tea, such as tea polyphenols, theanine and theaflavins, have good anti-inflammatory effects, and tea polyphenols have the best effect. However, antioxidant properties may not be the sole mechanism of anti-inflammatory. The mechanisms of anti-inflammatory are related with the comprehensive work of inhibiting inflammatory factor, modulate inflammatory signaling pathways, antioxidant, etc. This paper reviewed the anti-inflammatory effects of the main active components in tea and its mechanisms, and mainly emphasized the anti-inflammatory mechanism of catechin component epigallocatechin gallate(EGCG). It provided references for further study on the anti-inflammatory effects of tea, and a theoretical basis for the research and development of healthy tea drinking and tea anti-inflammatory products.
引文
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[35]Lin R,Chen C,Wang Y,et al.(-)-Epigallocatechin gallate inhibition of osteoclastic differentiation via NF-kappa B[J].Biochem Biophys Res Commun,2009,379(4):1033-1037.
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[2]Das M,Sur P,Gomes A,et al.Inhibition of tumour growth and inflammation by consumption of tea[J].Phytother Res,2002,16(S1):40-44.
[3]Chakrawarti L,Agrawal R,Dang S,et al.Therapeutic effects of EGCG:a patent review[J].Expert Opin Ther Pat,2016,26(8):907-916.
[4]Varilek GW,Yang F,Lee EY,et al.Green tea polyphenol extract attenuates inflammation in interleukin-2-deficient mice,a model of autoimmunity[J].J Nutr,2001,131(7):2034-2039.
[5]Yang H,Landis-Piwowar K,Chan TH,et al.Green tea polyphenols as proteasome inhibitors:implication in chemoprevention[J].Curr Cancer Drug Targets,2011,11(3):296-306.
[6]Shen C,Samathanam C,Tatum OL,et al.Green tea polyphenols avert chronic inflammation-induced myocardial fibrosis of female rats[J].Inflamm Res,2011,60(7):665-672.
[7]邓祖跃,康桦,辛艳飞,等.茶多酚对实验性动脉粥样硬化兔的血脂及炎症因子的影响[J].中国临床药理学与治疗学,2009(3):279-282.Deng ZY,Kang H,Xin YF,et al.Effects of tea polyphenols on the serum lipid and inflammation factor in experimental atherosclerosis rabbits[J].Chin J Clin Pharm Ther,2009(3):279-282.
[8]Qin B,Polansky MM,Harry D,et al.Green tea polyphenols improve cardiac muscle m RNA and protein levels of signal pathways related to insulin and lipid metabolism and inflammation in insulin-resistant rats[J].Mol Nutr Food Res,2010,54(S1):S14-S23.
[9]Peairs A,Dai R,Gan L,et al.Epigallocatechin-3-gallate(EGCG)attenuates inflammation in MRL/lpr mouse mesangial cells[J].Cell Mol Immunol,2010,7(2):123-132.
[10]Haqqi TM,Anthony DD,Gupta S,et al.Prevention of collagen-induced arthritis in mice by a polyphenolic fraction from green tea.[J].P Natl Acad Sci USA,1999,96(8):4524-4529.
[11]Sergent T,Piront N,Meurice J,et al.Anti-inflammatory effects of dietary phenolic compounds an in vitro model of inflamed human intestinal epithelium[J].Chem Biol Interact,2010,188(3):659-667.
[12]Yun H,Yoo W,Han M,et al.Epigallocatechin-3-gallate suppresses TNF-α-induced production of MMP-1 and-3 in rheumatoid arthritis synovial fibroblasts[J].Rheumatol Int,2008,29(1):23-29.
[13]Desai MJ,Gill MS,Hsu WH,et al.Pharmacokinetics of theanine enantiomers in rats[J].Chirality,2005,17(3):154-162.
[14]Liu Q,Duan H,Luan J,et al.Effects of theanine on growth of human lung cancer and leukemia cells as well as migration and invasion of human lung cancer cells[J].Cytotechnology,2009,59(3):211-217.
[15]Li G,Ye Y,Kang J,et al.l-Theanine prevents alcoholic liver injury through enhancing the antioxidant capability of hepatocytes[J].Food Chem Toxicol,2012,50(2):363-372.
[16]Zarse K,Jabin S,Ristow M.l-Theanine extends lifespan of adult Caenorhabditis elegans[J].Eur J Nutr,2012,51(6):765-768.
[17]火东晓,陈继华,张石蕊,等.L-茶氨酸对脂多糖诱导的巨噬细胞炎症细胞因子分泌的影响[J].营养学报,2014(5):499-501.Huo XD,Chen JH,Zhang SR,et al.Effect of L-theanine on inflammatory cytokines secretion of macrophoges induced by lipopolysaccharide in vitro[J].Acta Nutr Sin,2014(5):499-501.
[18]Kim NH,Jeong HJ,Kim HM.Theanine is a candidate amino acid for pharmacological stabilization of mast cells[J].Amino Acids,2012,42(5):1609-1618.
[19]Shibakusa T,Mikami T,Kurihara S,et al.Enhancement of postoperative recovery by preoperative oral co-administration of the amino acids,cystine and theanine,in a mouse surgical model[J].Clin Nutr,2012,31(4):555-561.
[20]祖勉,刘艾林,杜冠华.茶黄素及其衍生物药理作用研究进展[J].中国药学杂志,2011(20):1537-1541.Zu M,Liu AL,Du GY,et al.Research progress in pharmacological effects of theaflavins and their derivatives[J].Chin Pharm J,2011(20):1537-1541.
[21]牛丽,曹佩琴,刘仲华.茶黄素对葡萄糖诱导的人晶状体上皮细胞一氧化氮合成的影响[J].湖南农业大学学报(自然科学版),2014(4):369-372.Niu L,Cao PQ,Liu ZH,et al.Effect of TFDG on synthesis of NO in human lens epithelial cells by high glucose[J].J Hunan Agric Univ,2014(4):369-372.
[22]李红月,邓惠芳,戴长蓉,等.茶黄素对大鼠缺血性脑损伤所致炎症反应的作用[J].中国医院药学杂志,2016(20):1755-1759.Li YH,Deng HL,Dai CR,et al.Effect of theaflavin on inflammation caused by ischemic brain injures in rats[J].Chin Hosp Pharm J,2016(20):1755-1759.
[23]文秀芳,陈霞,邬海桥,等.茶黄素在大鼠气道上皮细胞炎症损伤中的作用及可能机制[J].重庆医学,2014(21):2756-2758.Wen XF,Chen X,Wu HQ,et al.Effect of theaflavin on LPS-induced inflammatory injury of rat airway epithelial cell and possible mechanism[J].Chongqin Med,2014(21):2756-2758.
[24]Gosslau A,Jao DL,Huang M,et al.Effects of the black tea polyphenol theaflavin-2 on apoptotic and inflammatory pathways in vitro and in vivo[J].Mol Nutr Food Res,2011,55(2):198-208.
[25]Crouvezier S,Powell B,Keir D,et al.The effects of phenolic components of tea on the production of pro-and anti-inflammatory cytokines by human leukocytes in vitro[J].Cytokine,2001,13:280-286.
[26]Rahman I,Biswas SK,Kirkham PA.Regulation of inflammation and redox signaling by dietary polyphenols[J].Biochem Pharm,2006,72(11):1439-1452.
[27]Wu D,Guo Z,Ren Z,et al.Green tea EGCG suppresses T cell proliferation through impairment of IL-2/IL-2 receptor signaling[J].Free Radical Biol Med,2009,47(5):636-643.
[28]Shen YJ,Li WG,Zhang H,et al.Effects of(-)-epigallocatechin-3-gallate on interleukin-1beta expression in mouse wound healing[J].J Mol Cell Biol,2009,42(3-4):179-185.
[29]Fujiki H,Suganuma M,Kurusu M,et al.New TNF-αreleasing inhibitors as cancer preventive agents from traditional herbal medicine and combination cancer prevention study with EGCG and sulindac or tamoxifen[J].Mutat Res-Fund Mol M,2003,523-524:119-125.
[30]Danesi F,Philpott M,Huebner C,et al.Food-derived bioactives as potential regulators of the IL-12/IL-23 pathway implicated in inflammatory bowel diseases[J].Mutat Res-Fund Mol M,2010,690(1-2):139-144.
[31]Zhong Y,Chiou Y,Pan M,et al.Anti-inflammatory activity of lipophilic epigallocatechin gallate(EGCG)derivatives in LPS-stimulated murine macrophages[J].Food Chem,2012,134(2):742-748.
[32]Singh R,Ahmed S,Islam N,et al.Epigallocatechin-3-gallate inhibits interleukin-1beta-induced expression of nitric oxide synthase and production of nitric oxide in human chondrocytes:suppression of nuclear factor kappa B activation by degradation of the inhibitor of nuclear factor kappa B[J].Arthritis Rheum,2002,46(8):2079-2086.
[33]Yang F,Oz HS,Barve S,et al.The green tea polyphenol(-)-epigallocatechin-3-gallate blocks nuclear factor-kappa B activation by inhibiting I kappa B kinase activity in the intestinal epithelial cell line IEC-6.[J].Mol Pharm,2001,60(3):528-533.
[34]Singh R,Akhtar N,Haqqi TM.Green tea polyphenol epigallocatechi3-gallate:Inflammation and arthritis[J].Life Sci,2010,86(25-26):907-918.
[35]Lin R,Chen C,Wang Y,et al.(-)-Epigallocatechin gallate inhibition of osteoclastic differentiation via NF-kappa B[J].Biochem Biophys Res Commun,2009,379(4):1033-1037.
[36]Ahmed S,Pakozdi A,Koch AE.Regulation of interleukin-1β–induced chemokine production and matrix metalloproteinase 2 activation by epigallocatechin-3-gallate in rheumatoid arthritis synovial fibroblasts[J].Arthritis Rheum,2006,54(8):2393-2401.
[37]Hafeez BB,Ahmed S,Wang N,et al.Green tea polyphenols-induced apoptosis in human osteosarcoma SAOS-2 cells involves a caspase-dependent mechanism with downregulation of nuclear factor-kappa B.[J].Toxicol Appl Pharm,2006,216(1):11-19.
[38]Lagha AB,Grenier D.Tea polyphenols inhibit the activation of NF-κB and the secretion of cytokines and matrix metalloproteinases by macrophages stimulated with Fusobacterium nucleatum[J].Sci Reports,2016,6(1).
[39]Syed DN,Afaq F,Kweon M,et al.Green tea polyphenol EGCG suppresses cigarette smoke condensate-induced NF-kappa B activation in normal human bronchial epithelial cells[J].Oncogene,2007,26(5):673-682.
[40]Lee JH,Jin H,Shim HE,et al.Epigallocatechin-3-gallate inhibits osteoclastogenesis by down-regulating c-Fos expression and suppressing the Nuclear Factor-B signal[J].Mol Pharm,2009,77(1):17-25.
[41]Yu L,Yu H,Li X,et al.P38 MAPK/mi R-1 are involved in the protective effect of EGCG in high glucose-induced Cx43 downregulation in neonatal rat cardiomyocytes[J].Cell Biol Int,2016,40(8):934-942.
[42]Bae J,Choi J,Choi Y,et al.(-)Epigallocatechin gallate hampers collagen destruction and collagenase activation in ultraviolet-B-irradiated human dermal fibroblasts:Involvement of mitogen-activated protein kinase[J].Food Chem Toxicol,2008,46(4):1298-1307.
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