基于下一代测序分析3种不同保鲜处理的香椿在贮藏期间微生物多样性变化
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摘要
香椿营养丰富,风味独特,深受消费者的喜爱,但容易腐烂变质,难以满足市场的需求。为了更好地提高其货架期,本研究以刚刚采摘的香椿嫩芽为试材,采用下一代测序技术比较不同保鲜方法下微生物的群落组成和多样性。结果表明,聚氯乙烯(polyvinyl chloride,PVC)保鲜膜结合紫外照射(ultraviolet irradiation,ZW)处理的香椿较聚氯乙烯保鲜膜、聚氯乙烯保鲜膜结合1-甲基环丙烯(1-methylcyclopropene,1-MCP)、聚氯乙烯保鲜膜结合乙烯吸附剂(ethylene absorbent,EA)处理的香椿的OTUs(Operational Taxonomic Units)和多样性均有所下降,保持优势的细菌门和真菌门分别为蓝菌门(Cyanobacteria)和子囊菌门(Ascomycota)。主成分分析表明,PVC+ZW处理组的微生物群落相似性更高,群落的稳定性更好。共现网络分析发现,紫外照射提高了不同菌群之间的负相关性。
Toona sinensis is rich in nutrition and has unique flavor,which is deeply loved by consumers,however,it is easy to rot and deteriorate,and hard to meet the needs of the market. In order to improve the shelf life of Toona sinensis,the next generation sequencing was used to compare the microbial communities and diversities under different preservation methods. The results showed that the OTUs and diversities of Toona sinensis treated with PVC+ZW were lower than those treated with PVC,PVC+1-MCP,and PVC+EA. The dominant bacteria and fungi were Cyanobacteria and Ascomycota,respectively. Principal component analysis showed that the microbial community of PVC + ZW treatment had higher similarity and better stability. Co-occurrence network analysis showed that ultraviolet irradiation could improve the negative correlation between microbial flora.
Toona sinensis is rich in nutrition and has unique flavor,which is deeply loved by consumers,however,it is easy to rot and deteriorate,and hard to meet the needs of the market. In order to improve the shelf life of Toona sinensis,the next generation sequencing was used to compare the microbial communities and diversities under different preservation methods. The results showed that the OTUs and diversities of Toona sinensis treated with PVC+ZW were lower than those treated with PVC,PVC+1-MCP,and PVC+EA. The dominant bacteria and fungi were Cyanobacteria and Ascomycota,respectively. Principal component analysis showed that the microbial community of PVC + ZW treatment had higher similarity and better stability. Co-occurrence network analysis showed that ultraviolet irradiation could improve the negative correlation between microbial flora.
引文
[1]Yang Y,Wang J,Xing Z E,et al.Identification of phenolics in Chinese toon and analysis of their content changes during storage[J].Food Chemistry,2011,128(4):831-838
[2]赵杰,赵芳,刘常金,等.“互联网+”背景下近郊香椿产业的发展路径分析[J].安徽农业科学,2017,45(13):232-234
[3]张仲平,赵惠英.香椿叶中黄酮成分及其含量的季节性变化研究[J].山东中医药大学学报,2001,25(2):141-142
[4]唐晓珍,李大鹏,孙淑静,等.香椿的贮藏与加工技术[J].食品工业科技,2002,23(11):94-95
[5]鲍琳,袁玉超,马晓跃,等.不同处理方法对香椿贮藏效果的研究[J].北方园艺,2006(4):37-38
[6]杨慧,毛维林,赵守涣,等.冰温结合开孔调湿包装对香椿嫩芽的保鲜效应[J].植物生理学通讯,2017,33(9):121-125
[7]王赵改,杨慧,朱广成.减压处理对香椿贮藏品质的影响研究[J].华北农学报,2013,28(6):181-185
[8]陈丽娟,杨金初,王赵改,等.外援甜菜碱对香椿嫩芽采后品质的影响[J].食品与生物技术学报,2015,34(12):1315-1320
[9]赵芳,孙晓健,于鹏飞,等.不同冷杀菌技术对香椿贮藏期品质的影响[J].食品研究与开发,2018,39(4):188-192
[10]Chitravathi K,Chauhan O P,Raju P S,et al.Efficacy of aqueous ozone and chlorine in combination with passive modified atmosphere packaging on the postharvest shelf-life extension of green chillies(Capsicum annuum L.)[J].Food and Bioprocess Technology,2015,8(6):1386-1392
[11]Gutierrez J,Bourke P,Lonchamp J,et al.Impact of plant essential oils on microbiological,organoleptic and quality markers of minimally processed vegetables[J].Innovative Food Science&Emerging Technologies,2009,10(2):195-202
[12]李恒,陈功,伍亚龙,等.高通量测序方法研究传统四川泡菜母水中微生物群落的动态变化[J].食品科学,2018,39(24):131-138
[13]Campanaro S,Treu L,Kougias P G,et al.Metagenomic binning reveals the functional roles of core abundant microorganisms in twelve full-scale biogas plants[J].Water Research,2018,140:123-134
[14]Frossard A,Donhauser J,Mestrot A,et al.Long-and short-term effects of mercury pollution on the soil microbiome[J].Soil Biology and Biochemistry,2018,120:191-199
[15]Fang H,Cai L,Yang Y,et al.Metagenomic analysis reveals potential biodegradation pathways of persistent pesticides in freshwater and marine sediments[J].Science of the Total Environment,2014,470/471:983-992
[16]Garrido-Oter R,Nakano R T,Dombrowski N,et al.Modular traits of the rhizobiales root Microbiota and their evolutionary relationship with symbiotic rhizobia[J].Cell Host&Microbe,2018,24(1):155-167
[17]Abdelfattah A,Wisniewski M,Droby S,et al.Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase[J].Horticulture Research,2016,3:16047
[18]Diskin S,Feygenberg O,Maurer D,et al.Microbiome alterations are correlated with occurrence of postharvest stem-end rot in mango fruit[J].Phytobiomes Journal,2017,1(3):117-127
[19]Magoc T,Salzberg S L.FLASH:fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2957-2963
[20]Caporaso J G,Kuczynski J,Stombaugh J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336
[21]Quast C,Pruesse E,Yilmaz P,et al.The SILVA ribosomal RNAgene database project:improved data processing and web-based tools[J].Nucleic Acids Research,2012,41(1):590-596
[22]Cheon H L,Shin J Y,Park K H,et al.Inactivation of foodborne pathogens in powdered red pepper(Capsicum annuum L.)using combined UV-C irradiation and mild heat treatment[J].Food Control,2015,50:441-445
[23]郑杨,曹敏,申琳,等.短波紫外线照射对韭菜采后贮藏品质及活性氧代谢相关酶的影响[J].食品科学,2011,32(20):307-311
[24]Choi E J,Park H W,Yang H S,et al.Effects of combined treatment with ultraviolet-C irradiation and grape seed extract followed by supercooled storage on microbial inactivation and quality of dongchimi[J].LWT-Food Science and Technology,2017,85:110-120
[25]Hamilton T L,Bryant D A,MacAlady J L.The role of biology in planetary evolution:cyanobacterial primary production in low-oxygen Proterozoic oceans[J].Environmental Microbiology,2016,18(2):325-340
[26]Wolińska A,Kuzniar A,Zielenkiewicz U,et al.Indicators of arable soils fatigue-Bacterial families and genera:A metagenomic approach[J].Ecological Indicators,2018,93:490-500
[27]Glowacz M,Rees D.The practicality of using ozone with fruit and vegetables[J].Journal of the Science of Food and Agriculture,2016,96(14):4637-4643
[28]Binder M,Justo A,Riley R,et al.Phylogenetic and phylogenomic overview of the Polyporales[J].Mycologia,2013,105(6):1350-1373
[29]Jiao S,Liu Z S,Lin Y B,et al.Bacterial communities in oil contaminated soils:Biogeography and co-occurrence patterns[J].Soil Biology and Biochemistry,2016,98:64-73
[2]赵杰,赵芳,刘常金,等.“互联网+”背景下近郊香椿产业的发展路径分析[J].安徽农业科学,2017,45(13):232-234
[3]张仲平,赵惠英.香椿叶中黄酮成分及其含量的季节性变化研究[J].山东中医药大学学报,2001,25(2):141-142
[4]唐晓珍,李大鹏,孙淑静,等.香椿的贮藏与加工技术[J].食品工业科技,2002,23(11):94-95
[5]鲍琳,袁玉超,马晓跃,等.不同处理方法对香椿贮藏效果的研究[J].北方园艺,2006(4):37-38
[6]杨慧,毛维林,赵守涣,等.冰温结合开孔调湿包装对香椿嫩芽的保鲜效应[J].植物生理学通讯,2017,33(9):121-125
[7]王赵改,杨慧,朱广成.减压处理对香椿贮藏品质的影响研究[J].华北农学报,2013,28(6):181-185
[8]陈丽娟,杨金初,王赵改,等.外援甜菜碱对香椿嫩芽采后品质的影响[J].食品与生物技术学报,2015,34(12):1315-1320
[9]赵芳,孙晓健,于鹏飞,等.不同冷杀菌技术对香椿贮藏期品质的影响[J].食品研究与开发,2018,39(4):188-192
[10]Chitravathi K,Chauhan O P,Raju P S,et al.Efficacy of aqueous ozone and chlorine in combination with passive modified atmosphere packaging on the postharvest shelf-life extension of green chillies(Capsicum annuum L.)[J].Food and Bioprocess Technology,2015,8(6):1386-1392
[11]Gutierrez J,Bourke P,Lonchamp J,et al.Impact of plant essential oils on microbiological,organoleptic and quality markers of minimally processed vegetables[J].Innovative Food Science&Emerging Technologies,2009,10(2):195-202
[12]李恒,陈功,伍亚龙,等.高通量测序方法研究传统四川泡菜母水中微生物群落的动态变化[J].食品科学,2018,39(24):131-138
[13]Campanaro S,Treu L,Kougias P G,et al.Metagenomic binning reveals the functional roles of core abundant microorganisms in twelve full-scale biogas plants[J].Water Research,2018,140:123-134
[14]Frossard A,Donhauser J,Mestrot A,et al.Long-and short-term effects of mercury pollution on the soil microbiome[J].Soil Biology and Biochemistry,2018,120:191-199
[15]Fang H,Cai L,Yang Y,et al.Metagenomic analysis reveals potential biodegradation pathways of persistent pesticides in freshwater and marine sediments[J].Science of the Total Environment,2014,470/471:983-992
[16]Garrido-Oter R,Nakano R T,Dombrowski N,et al.Modular traits of the rhizobiales root Microbiota and their evolutionary relationship with symbiotic rhizobia[J].Cell Host&Microbe,2018,24(1):155-167
[17]Abdelfattah A,Wisniewski M,Droby S,et al.Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase[J].Horticulture Research,2016,3:16047
[18]Diskin S,Feygenberg O,Maurer D,et al.Microbiome alterations are correlated with occurrence of postharvest stem-end rot in mango fruit[J].Phytobiomes Journal,2017,1(3):117-127
[19]Magoc T,Salzberg S L.FLASH:fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2957-2963
[20]Caporaso J G,Kuczynski J,Stombaugh J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336
[21]Quast C,Pruesse E,Yilmaz P,et al.The SILVA ribosomal RNAgene database project:improved data processing and web-based tools[J].Nucleic Acids Research,2012,41(1):590-596
[22]Cheon H L,Shin J Y,Park K H,et al.Inactivation of foodborne pathogens in powdered red pepper(Capsicum annuum L.)using combined UV-C irradiation and mild heat treatment[J].Food Control,2015,50:441-445
[23]郑杨,曹敏,申琳,等.短波紫外线照射对韭菜采后贮藏品质及活性氧代谢相关酶的影响[J].食品科学,2011,32(20):307-311
[24]Choi E J,Park H W,Yang H S,et al.Effects of combined treatment with ultraviolet-C irradiation and grape seed extract followed by supercooled storage on microbial inactivation and quality of dongchimi[J].LWT-Food Science and Technology,2017,85:110-120
[25]Hamilton T L,Bryant D A,MacAlady J L.The role of biology in planetary evolution:cyanobacterial primary production in low-oxygen Proterozoic oceans[J].Environmental Microbiology,2016,18(2):325-340
[26]Wolińska A,Kuzniar A,Zielenkiewicz U,et al.Indicators of arable soils fatigue-Bacterial families and genera:A metagenomic approach[J].Ecological Indicators,2018,93:490-500
[27]Glowacz M,Rees D.The practicality of using ozone with fruit and vegetables[J].Journal of the Science of Food and Agriculture,2016,96(14):4637-4643
[28]Binder M,Justo A,Riley R,et al.Phylogenetic and phylogenomic overview of the Polyporales[J].Mycologia,2013,105(6):1350-1373
[29]Jiao S,Liu Z S,Lin Y B,et al.Bacterial communities in oil contaminated soils:Biogeography and co-occurrence patterns[J].Soil Biology and Biochemistry,2016,98:64-73