重庆地区取食高粱的草地贪夜蛾与玉米粘虫肠道细菌比较
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摘要
草地贪夜蛾(Spodoptera frugiperda)于2019年5月被发现已经入侵至重庆地区, 6月底在江津地区的高粱地中也发现了少量的草地贪夜蛾,同时还发现了大量玉米粘虫(Mythimna seperata).通过分离培养同一生境内的草地贪夜蛾和玉米粘虫的肠道细菌,结合16S rDNA测序完成了这两种害虫肠道优势菌属水平的鉴定,共分离得到了19个细菌分离株,其中分离自草地贪夜蛾肠道细菌归为5个属,分别为欧文氏菌属(Erwinia),不动杆菌属(Acinetobacter),肠杆菌属(Enterobacter),肠球菌属(Enterococcus),假单胞菌属(Pseudomonas).分离自玉米粘虫肠道细菌归为6个属,分别为克雷伯氏菌属(Klebsiella),不动杆菌属(Acinetobacter),寡养单胞菌属(Stenotrophomonas),肠球菌属(Enterococcus),金黄杆菌属(Chryseobacterium),芽孢杆菌属(Bacillus).同时我们也对采自玉米地和高粱地的草地贪夜蛾肠道微生物优势菌进行了比较分析,在江津高粱地中的草地贪夜蛾中没有发现克雷伯氏菌,但该菌在巫山、巫溪玉米地的草地贪夜蛾中大量存在.结论为进一步研究草地贪夜蛾幼虫肠道核心微生物群奠定了基础.
In May of 2019, Spodoptera frugiperda migrated to Chongqing and spread rapidly. It was found in maize fields in several counties and districts. At the end of June, a little S. frugiperda and plenty Mythimna seperata were found in sorghum fields in Jiangjin district. In this study we isolated the intestinal dominant bacteria from the larvae of S. frugiperda and M. seperata, and used 16 S rDNA sequencing to identify their dominant genera. In total, we obtained 19 isolates of bacteria, of which Erwinia, Acinetobacter, Enterobacter, Enterococcus and Pseudomonas were identified from S. frugiperda; Klebsiella, Acinetobacter, Stenotrophomonas, Enterococcus, Chryseobacterium and Bacillus were idenfified from M. seperata. The dominant bacteria types in the gut of the two insects in the same habitat were obviously diffierent although two genera were same. At the same time, we also conducted a comparative analysis of the dominant intestinal bacteria of S. frugiperda in the maize field and the sorghum field. We did not find Klebsiella sp. in S. frugiperda feeding on sorghum in Jiangjin district, which was abundant in the S. frugiperda feeding on maize in Wushan and Wuxi. This work may lay a foundation for further study on the core intestinal microorganisms of the S. frugiperda larvae.
In May of 2019, Spodoptera frugiperda migrated to Chongqing and spread rapidly. It was found in maize fields in several counties and districts. At the end of June, a little S. frugiperda and plenty Mythimna seperata were found in sorghum fields in Jiangjin district. In this study we isolated the intestinal dominant bacteria from the larvae of S. frugiperda and M. seperata, and used 16 S rDNA sequencing to identify their dominant genera. In total, we obtained 19 isolates of bacteria, of which Erwinia, Acinetobacter, Enterobacter, Enterococcus and Pseudomonas were identified from S. frugiperda; Klebsiella, Acinetobacter, Stenotrophomonas, Enterococcus, Chryseobacterium and Bacillus were idenfified from M. seperata. The dominant bacteria types in the gut of the two insects in the same habitat were obviously diffierent although two genera were same. At the same time, we also conducted a comparative analysis of the dominant intestinal bacteria of S. frugiperda in the maize field and the sorghum field. We did not find Klebsiella sp. in S. frugiperda feeding on sorghum in Jiangjin district, which was abundant in the S. frugiperda feeding on maize in Wushan and Wuxi. This work may lay a foundation for further study on the core intestinal microorganisms of the S. frugiperda larvae.
引文
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[2] 向芸庆,王晓强,冯伟,等.不同饲料饲养家蚕其肠道微生态优势菌群类型的组成及差异性 [J].生态学报,2010,30(14):3875-3882.
[3] CHEN B S,DU K Q,SUN C,et al.Gut Bacterial and Fungal Communities of the Domesticated Silkworm (Bombyx mori) and Wild Mulberry-Feeding Relatives [J].The ISME Journal,2018,12(9):2252-2262.
[4] 陈冬平,陈志廷,徐汉虹,等.不同分子标记对入侵广州草地贪夜蛾的寄主型鉴别能力比较 [J/OL].华南农业大学学报:1-5[2019-07-12].http://kns.cnki.net/kcms/detail/44.1110.S.20190617.1027.002.html.
[5] 秦誉嘉,蓝帅,赵紫华,等.迁飞性害虫草地贪夜蛾在我国的潜在地理分布 [J/OL].植物保护:1-8[2019-07-12].https://doi.org/10.16688/j.zwbh.2019269.
[6] 张磊,靳明辉,张丹丹,等.入侵云南草地贪夜蛾的分子鉴定 [J].植物保护,2019,45(2):19-24,56.
[7] 齐国君,马健,胡高,等.首次入侵广东的草地贪夜蛾迁入路径及天气背景分析 [J].环境昆虫学报,2019,41(3):488-496.
[8] ACEVEDO F E,PEIFFER M,TAN C W,et al.Fall Armyworm-Associated Gut Bacteria Modulate Plant Defense Responses [J].Molecular Plant-Microbe Interactions,2017,30(2):127-137.
[9] OLIVEIRA P L.The Gut Microbiota of Insecticide-Resistant Insects Houses Insecticide-Degrading Bacteria:A Potential Source for Biotechnological Exploitation [J].PLoS One,2017,12(3):e0174754.
[10] 唐运林,顾偌铖,吴燕燕,等.入侵重庆地区的草地贪夜蛾种群生物型鉴定 [J].西南大学学报(自然科学版),2019,41(7):1-7.
[11] 唐运林,吴燕燕,顾偌铖,等.重庆地区草地贪夜蛾肠道细菌的分离鉴定 [J].西南大学学报(自然科学版),2019,41(7):8-14.
[12] 李玉娟,苏琬真,胡坤坤,等.植物乳杆菌促进黑腹果蝇生长发育 [J].昆虫学报,2017,60(5):544-552.
[13] SCHRETTER C E,VIELMETTER J,BARTOS I,et al.A Gut Microbial Factor Modulates Locomotor Behaviour in Drosophila [J].Nature,2018,563(7731):402-406.
[14] IATSENKO I,BOQUETE J P,LEMAITRE B.Microbiota-Derived Lactate Activates Production of Reactive Oxygen Species by the Intestinal NADPH Oxidase Nox and Shortens Drosophila Lifespan [J].Immunity,2018,49(5):929-942.
[15] 宋月,李娜,岳莹雪,等.膳食中的主要成分对肠道微生物组成及代谢影响的研究进展 [J/OL].食品工业科技:1-9[2019-07-12].http://kns.cnki.net/kcms/detail/11.1759.ts.20190506.1637.035.html.
[16] 刘春蕾.不同糖源饲料对越冬蜜蜂肠道健康及肠道微生物区系的影响 [D].泰安:山东农业大学,2017.
[17] HE C,NAN X N,ZHANG Z Q,et al.Composition and Diversity Analysis of the Gut Bacterial Community of the Oriental Armyworm,Mythimna Separata,Determined by Culture-Independent and Culture-Dependent Techniques [J].Journal of Insect Science,2013,13(165):1-11.
[18] HANNULA S E,ZHU F,HEINEN R,et al.Foliar-Feeding Insects Acquire Microbiomes from the Soil rather than the Host Plant [J].Nature Communications,2019(10):1254.