基于肺癌干细胞理论探讨中医药在肺癌治疗中的应用
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摘要
肿瘤干细胞(cancer stem cells,CSCs)是恶性肿瘤中存在一群数量微小、具有自我更新和多向分化潜能的特殊细胞亚群,是肿瘤不断生长以及侵袭转移的根源。中医药在肺癌综合治疗中占有不可忽视地位,可抑制肺癌发生发展。基于此,文章阐明了肺癌干细胞在肺癌中的作用,总结探讨中药单药、中药复方、中成药在抑制肺癌干细胞阻滞肺癌进展中的作用及相关机制,以期为肺癌治疗提供依据与策略。
Cancer stem cells( CSCs) are a subgroup of minor cells in a malignant tumor with the potentials of self-renewing and multi-directional differentiation. It is the root cause for continuous growth of tumor,as well as invasion and metastasis of tumor. TCM plays an unignorable role in treatment of lung cancer,and it can inhibit lung cancer development. This study illustrated the role of lung cancer stem cells( LCSCs) in lung cancer,and summarized and discussed the effect and mechanism of single herb,compound formula and patent medicine on LCSCs to prevent metastasis of lung cancer,aiming to provide guiding basis for the treatment.
Cancer stem cells( CSCs) are a subgroup of minor cells in a malignant tumor with the potentials of self-renewing and multi-directional differentiation. It is the root cause for continuous growth of tumor,as well as invasion and metastasis of tumor. TCM plays an unignorable role in treatment of lung cancer,and it can inhibit lung cancer development. This study illustrated the role of lung cancer stem cells( LCSCs) in lung cancer,and summarized and discussed the effect and mechanism of single herb,compound formula and patent medicine on LCSCs to prevent metastasis of lung cancer,aiming to provide guiding basis for the treatment.
引文
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[15]Koren A,Rijavec M,Kern I,et al.BMI1,ALDH1A1,and CD133 transcripts connect epithelial-mesenchymal transition to cancer stem cells in lung carcinoma[J].Stem Cells Int,2016,10(7):1155-1160.
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[23]王耀焓.苏木、川芎在体内模型中对肺癌干细胞样细胞的干预作用及机制研究[D].北京:中国中医科学院,2016.
[24]盛良翩.高乌甲素联合多西他赛和奥沙利铂抑制肺癌的机制研究[D].广州:暨南大学,2010.
[25]谢有科,张弓,李雪梅,等.莱菔硫烷抑制肺癌干细胞增殖的体外实验[J].肿瘤防治研究,2016,43(7):555-559.
[26]Zhong D,Zhang HJ,Jiang YD,et al.Saikosaponin-d∶A potential chemotherapeutics in castration resistantprostate cancer by suppressing cancermetastases and cancer stem cellphenotypes[J].Biochem Biophys Res Commun,2016,474(4)∶722-729.
[27]Jung Y,Park H,Zhao HY,et al.Systemic approaches identify a garlic-derived chemical,Z-ajoene,as a glioblastoma multiforme cancer stem cell-spe-cific targeting agent[J].Mol Cells,2014,37(7):547-553.
[28]郑展,王菊勇,徐振晔.肺岩宁颗粒干预细胞周期G1/S检测点调控信号抗Lewis肺癌细胞增殖的研究[J].中西医结合杂志,2009,29(11):1018-1102.
[29]王爽,王立芳,徐振晔,等.肺炎宁方对肺癌干细胞Wnt信号通路的影响[J].上海中医药大学学报,2012,26(6):77-82.
[30]肖桦,秦旭华,赖宇,等.西黄丸含药血清经Wnt信号转导通路细胞周期蛋白D1调控人肺癌干细胞增殖的机制[J].中国实验方剂学杂志,2014,20(15)∶172-176.
[31]孙建立,刘嘉湘.金复康口服液对裸鼠人肺腺癌细胞凋亡相关基因表达的影响[J].上海中医药杂志,2007,41(10):69-71.
[32]孙建立,孙玺媛,刘嘉湘.金复康口服液对耐药人肺腺癌A549/DDP膜转运蛋白LRP MRPmRNA表达的影响[J].辽宁中医药大学学报,2009,11(7):210-213.
[33]李春杰,孙建立,刘苓霜,等.益气养阴方调节C57小鼠Lewis肺癌MMP-9,TIMP-1表达和抗肺转移的实验研究[J].中华中医药学刊,2009,27(7):1444-1446.
[34]徐蔚杰,李春杰,刘嘉湘,等.金复康口服液抑制Lewis肺癌Sca-1+干细胞亚群增殖的研究[J].上海中医药杂志,2015,49(1):69-71.
[35]石锦萍,钟柏松,高丽元,等.乌三颗粒体外对恶性肿瘤细胞核酸合成的抑制作用[J].中国中西医结合杂志,2003,23(理论研究特集):142-143.
[36]徐振晔,龚亚斌.未来10余年肺癌中西医诊疗展望[J].上海中医药大学学报,2011,25(1):10-14.
[37]熊思会,万莎,谭溧,等.中医药调控肿瘤干细胞的研究现状[J].世界中医药,2017,12(10):2535-2538.
[38]张瀑,尹翮翔,刘福国,等.肺癌干细胞的研究进程与展望[J].癌症进展,2013,11(5):444-448.
[39]张琦,毕红霞,罗春娟.中医中药在肺癌干细胞治疗领域的应用[J].齐齐哈尔医学院学报,2015,36(1):87-88.
[2]Hamburger A W,Salmon S E.Primary bioassay of human tumor stem cells[J].Science,1977,197(4302):461-463.
[3]Reya T,Morrison SJ,Clarke MF,et al.Stem cells,cancer,and cancer stem cells[J].Nature,2001,414(6859):105-111.
[4]喻杨,王喻义,王乙钦,等.肿瘤干细胞与肿瘤微环境相互作用的研究进展[J].华西医学,2015,30(9):1784-1788.
[5]唐为安,徐兴祥,杨俊俊.微环境中不同因素对肺癌干细胞影响的研究进展[J].山东医药,2017,57(38):99-102.
[6]Prieto-Vila M,Yan T,Calle AS,et al.IPSC-derived cancer stem cells provide a model of tumor vasculature[J].Am J Cancer Res,2016,6(9):1906-1921.
[7]Kéramidas M,de Fraipont F,Karageorgis A,et al.The dual effect of mesenchymal stem cells on tumour growth and tumour angiogenesis[J].Stem Cell Res Ther,2013,4(2):41-48.
[8]Stratigos M,Matikas A,Voutsina A,et al.Targeting angio-genesis in small cell lung cancer[J].Transl Lung Cancer Res,2016,5(4):389-400.
[9]Singh S,Chellappan S.Lung cancer stem cells:molecular features and therapeutic targets[J].Mol Aspects Med,2014,39(10):50-60.
[10]李慧杰,齐元富,李秀荣.肺癌干细胞与肺癌转移相关机制的研究进展[J].中国肿瘤,2017,26(10):798-801.
[11]Ji W,Yu Y,Li Z,et al.FGFR1 promotes the stem cell-like phenotype of FGFR1-amplified non-small cell lung cancer cells through the Hedgehog pathway[J].Oncotarget,2016,7(12):15118-15134.
[12]张瀑,尹翮翔,刘福国,等.肺癌干细胞的研究进程与展望[J].癌症进展,2013,11(5):444-448.
[13]韩帅.发生上皮间质化的循环肿瘤干细胞在肺癌增殖、耐药及骨转移过程中相关作用的研究[D].上海:第二军医大学,2016.
[14]Miao L,Huang Z,Zengli Z,et al.Loss of long noncoding RNA FOXF1-AS1 regulates epithelial-mesenchymal transition,stemness and metastasis of non-small cell lung cancer cells[J].Oncotarget,2016,7(42):68339-68349.
[15]Koren A,Rijavec M,Kern I,et al.BMI1,ALDH1A1,and CD133 transcripts connect epithelial-mesenchymal transition to cancer stem cells in lung carcinoma[J].Stem Cells Int,2016,10(7):1155-1160.
[16]Takahashi K,Yamanaka S.Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors[J].Cell,2006,126(4):663-676.
[17]Suva ML,Riggi N,Bernstein BE.Epigenere programming in cancer[J].Science,2013,339(27):1567-1570.
[18]Chen YC,Hsu Hs,Chen YW,et al.Oct-4 expression maintained cancer stem-like properties in respiratory epithelium tumorigenesis in lung cancer-derived CD133-positive cells[J].Plo S One,2008,3(7):e2637.
[19]李小江,张文治,张莹,等.肺癌干细胞的影响机制研究进展[J].中国肿瘤临床,2012,39(9):619-622.
[20]李小江,贾英杰,张文治,等.中医药对肺癌干细胞干预作用的研究概况[J].中医杂志,2012,53(8):708-710.
[21]徐志伟,张进,张明.从心肾相关探讨干细胞移植治疗冠心病心衰的中医理论内涵[J].云南中医学院学报,2009,32(5):1-4.
[22]Chang K J,Yang M H,Zheng J C,et al.Arsenic trioxide inhibits cancer stem-like cells via down-regulation of Gli1 in lung cancer[J].American Journal of Translational Research,2016,8(2):1133-1143.
[23]王耀焓.苏木、川芎在体内模型中对肺癌干细胞样细胞的干预作用及机制研究[D].北京:中国中医科学院,2016.
[24]盛良翩.高乌甲素联合多西他赛和奥沙利铂抑制肺癌的机制研究[D].广州:暨南大学,2010.
[25]谢有科,张弓,李雪梅,等.莱菔硫烷抑制肺癌干细胞增殖的体外实验[J].肿瘤防治研究,2016,43(7):555-559.
[26]Zhong D,Zhang HJ,Jiang YD,et al.Saikosaponin-d∶A potential chemotherapeutics in castration resistantprostate cancer by suppressing cancermetastases and cancer stem cellphenotypes[J].Biochem Biophys Res Commun,2016,474(4)∶722-729.
[27]Jung Y,Park H,Zhao HY,et al.Systemic approaches identify a garlic-derived chemical,Z-ajoene,as a glioblastoma multiforme cancer stem cell-spe-cific targeting agent[J].Mol Cells,2014,37(7):547-553.
[28]郑展,王菊勇,徐振晔.肺岩宁颗粒干预细胞周期G1/S检测点调控信号抗Lewis肺癌细胞增殖的研究[J].中西医结合杂志,2009,29(11):1018-1102.
[29]王爽,王立芳,徐振晔,等.肺炎宁方对肺癌干细胞Wnt信号通路的影响[J].上海中医药大学学报,2012,26(6):77-82.
[30]肖桦,秦旭华,赖宇,等.西黄丸含药血清经Wnt信号转导通路细胞周期蛋白D1调控人肺癌干细胞增殖的机制[J].中国实验方剂学杂志,2014,20(15)∶172-176.
[31]孙建立,刘嘉湘.金复康口服液对裸鼠人肺腺癌细胞凋亡相关基因表达的影响[J].上海中医药杂志,2007,41(10):69-71.
[32]孙建立,孙玺媛,刘嘉湘.金复康口服液对耐药人肺腺癌A549/DDP膜转运蛋白LRP MRPmRNA表达的影响[J].辽宁中医药大学学报,2009,11(7):210-213.
[33]李春杰,孙建立,刘苓霜,等.益气养阴方调节C57小鼠Lewis肺癌MMP-9,TIMP-1表达和抗肺转移的实验研究[J].中华中医药学刊,2009,27(7):1444-1446.
[34]徐蔚杰,李春杰,刘嘉湘,等.金复康口服液抑制Lewis肺癌Sca-1+干细胞亚群增殖的研究[J].上海中医药杂志,2015,49(1):69-71.
[35]石锦萍,钟柏松,高丽元,等.乌三颗粒体外对恶性肿瘤细胞核酸合成的抑制作用[J].中国中西医结合杂志,2003,23(理论研究特集):142-143.
[36]徐振晔,龚亚斌.未来10余年肺癌中西医诊疗展望[J].上海中医药大学学报,2011,25(1):10-14.
[37]熊思会,万莎,谭溧,等.中医药调控肿瘤干细胞的研究现状[J].世界中医药,2017,12(10):2535-2538.
[38]张瀑,尹翮翔,刘福国,等.肺癌干细胞的研究进程与展望[J].癌症进展,2013,11(5):444-448.
[39]张琦,毕红霞,罗春娟.中医中药在肺癌干细胞治疗领域的应用[J].齐齐哈尔医学院学报,2015,36(1):87-88.