不同分型腺性膀胱炎中CK20、Ki67、Ras P21蛋白表达及临床意义研究
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摘要
目的探讨CK20、Ki67、Ras P21蛋白三种肿瘤标志物在正常膀胱组织、不同类型腺性膀胱炎及膀胱腺癌组织中表达的特点。方法将腺性膀胱炎经典型(泌尿上皮型)20例、肠上皮型20例、前列腺上皮型20例,正常膀胱组织20例、膀胱腺癌组织20例,采用免疫组织化学法,检查CK20、Ki67、Ras P21在各分型腺性膀胱炎中的表达,并与正常膀胱组织、膀胱腺癌组织组进行比较。结果肠上皮型和前列腺上皮型腺性膀胱炎CK20、Ki67、Ras P21阳性表达与正常膀胱黏膜组织比较,差异具有统计学意义(P<0.05)。而经典型(泌尿上皮型)CK20、Ki67、Ras P21的表达与正常膀胱黏膜组织比较,差异无统计学意义(P>0.05),肠上皮型和前列腺上皮型腺性膀胱炎及膀胱腺癌中CK20、Ki67、Ras P21高表达。肠上皮型和前列腺上皮型腺性膀胱炎可能为癌前病变。结论对于肠上皮型及前列腺型腺性膀胱炎,应进行积极的手术治疗及抗癌药物膀胱灌注,并严密术后随访。
Objective To investigate the expression of three tumor markers of CK20, Ki67 and Ras P21 in normal bladder tissues, different types of glandular cystitis and bladder adenocarcinoma. Methods 10 cases of classic cystitis(urinary epithelial type), 10 cases of intestinal epithelium, 10 cases of prostate epithelium, 10 cases of normal bladder tissue and 10 cases of bladder adenocarcinoma were examined by immunohistochemistry. The expression of Ki67 and Ras P21 in each glandular cystitis was compared with normal bladder tissue and bladder adenocarcinoma. Results The positive expressions of CK20, Ki67 and Ras P21 in intestinal epithelial type and prostatic epithelial glandular cystitis were significantly different from those in normal bladder mucosa(P<0.05). The expression of classic(urinary epithelial) CK20, Ki67, Ras P21 was not significantly different from normal bladder mucosa(P >0.05), intestinal epithelial and prostatic epithelial cystitis and bladder adenocarcinoma. CK20, Ki67, and Ras P21 are highly expressed. Intestinal epithelial and prostatic epithelial glandular cystitis may be precancerous lesions. Conclusion For intestinal epithelial and prostatic glandular cystitis, active surgical treatment and anti-cancer drug bladder infusion should be performed and followed up closely.
Objective To investigate the expression of three tumor markers of CK20, Ki67 and Ras P21 in normal bladder tissues, different types of glandular cystitis and bladder adenocarcinoma. Methods 10 cases of classic cystitis(urinary epithelial type), 10 cases of intestinal epithelium, 10 cases of prostate epithelium, 10 cases of normal bladder tissue and 10 cases of bladder adenocarcinoma were examined by immunohistochemistry. The expression of Ki67 and Ras P21 in each glandular cystitis was compared with normal bladder tissue and bladder adenocarcinoma. Results The positive expressions of CK20, Ki67 and Ras P21 in intestinal epithelial type and prostatic epithelial glandular cystitis were significantly different from those in normal bladder mucosa(P<0.05). The expression of classic(urinary epithelial) CK20, Ki67, Ras P21 was not significantly different from normal bladder mucosa(P >0.05), intestinal epithelial and prostatic epithelial cystitis and bladder adenocarcinoma. CK20, Ki67, and Ras P21 are highly expressed. Intestinal epithelial and prostatic epithelial glandular cystitis may be precancerous lesions. Conclusion For intestinal epithelial and prostatic glandular cystitis, active surgical treatment and anti-cancer drug bladder infusion should be performed and followed up closely.
引文
[1]王宁,叶章群,陈志强.腺性膀胱炎的新认识[J].中华泌尿外科杂志,2017,38(3):235-237.
[2]姜涛,张旭,雷光辉,等.细胞角蛋白7、20在女性腺性膀胱炎中的表达及临床意义[J].解放军预防医学杂志,2018,36(3):372-374.
[3]王洪志,熊波,聂志林,等.CK20、Ki67、Ras P21蛋白在腺性膀胱炎和膀胱腺癌中的表达及意义[J].医学信息,2015,28(44):153-154.
[4]李惠长,钱本江.EphA2在人膀胱移行细胞癌中的表达及其临床意义[J].中国肿瘤临床与康复,2009,16(3):199-204.
[5]张庆松,李传昌,陈忠,等.小分子双链RNAs联合应用抑制膀胱癌细胞增殖的实验研究[J].现代泌尿生殖肿瘤杂志,2016,8(4):225-230.
[6]Rau AR,Kini H,Pai RR.Morphological evaluation of cystitis glan-dularis[J].Indian J Pathol Microbiol,2009,52(2):203.
[2]姜涛,张旭,雷光辉,等.细胞角蛋白7、20在女性腺性膀胱炎中的表达及临床意义[J].解放军预防医学杂志,2018,36(3):372-374.
[3]王洪志,熊波,聂志林,等.CK20、Ki67、Ras P21蛋白在腺性膀胱炎和膀胱腺癌中的表达及意义[J].医学信息,2015,28(44):153-154.
[4]李惠长,钱本江.EphA2在人膀胱移行细胞癌中的表达及其临床意义[J].中国肿瘤临床与康复,2009,16(3):199-204.
[5]张庆松,李传昌,陈忠,等.小分子双链RNAs联合应用抑制膀胱癌细胞增殖的实验研究[J].现代泌尿生殖肿瘤杂志,2016,8(4):225-230.
[6]Rau AR,Kini H,Pai RR.Morphological evaluation of cystitis glan-dularis[J].Indian J Pathol Microbiol,2009,52(2):203.