水杨酸抑制生长素运输而调节Acuce水稻根的生长
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摘要
【目的】根系作为植物生长发育必不可少的器官,直接影响地上部分的产量、品质、抗性等诸多优良的农艺性状。该项研究主要探究水杨酸(SA)影响月亮谷水稻根系发育与生长素之间的关系。【方法】用含有不同浓度SA的培养基培养水稻材料,观察根表型;重力刺激实验以及用生长素运输抑制剂Naphthyl phthalamic acid (NPA)处理水稻材料,分析SA影响水稻根系发育与生长素的关系;利用细胞生物学实验观察水稻根系的胞吞作用。【结果】在含不同浓度SA的培养基中生长的水稻材料,其胚根、侧根、不定根的生长发育都会受到影响。重力刺激实验以及用生长素运输抑制剂NPA处理水稻材料,结果显示SA影响水稻根系发育与生长素的运输有关。SA还抑制膜蛋白的胞吞。【结论】水杨酸可通过抑制膜蛋白的胞吞从而改变生长素极性运输,进而影响水稻根系的生长发育。
【Objective】As an indispensable organ for plant growth and development, root system directly regulates some agronomic traits of aboveground parts including yield, quality and resistance. This study focused on the relationship between salicylic acid(SA) and auxin in regulating root development of Acuce rice.【Method】 Root phenotype of Acuce rice was observed by culture rice seedling grown on medium complemented with different concentrations of SA. The effect of SA and auxin cross-talk on the development of rice root was analyzed under gravity stimulation experiment and treated with auxin transport inhibitor Naphthyl phthalamic acid(NPA). Cell endocytosis of rice root was developed with cytological experiment. 【Result】Different concentration of SA treatment inhibited the growth of primary root, lateral root and adventitious root. SA regulated rice root development was connected with the disturbance of auxin transport under gravity stimulation and auxin transport inhibitor NPA treatment. Furthermore, SA inhibited cell endocytosis of plasma membrane proteins. 【Conclusion】 Salicylic acid could inhibit cell endocytosis to disturb polar auxin transport to regulate rice root development.
【Objective】As an indispensable organ for plant growth and development, root system directly regulates some agronomic traits of aboveground parts including yield, quality and resistance. This study focused on the relationship between salicylic acid(SA) and auxin in regulating root development of Acuce rice.【Method】 Root phenotype of Acuce rice was observed by culture rice seedling grown on medium complemented with different concentrations of SA. The effect of SA and auxin cross-talk on the development of rice root was analyzed under gravity stimulation experiment and treated with auxin transport inhibitor Naphthyl phthalamic acid(NPA). Cell endocytosis of rice root was developed with cytological experiment. 【Result】Different concentration of SA treatment inhibited the growth of primary root, lateral root and adventitious root. SA regulated rice root development was connected with the disturbance of auxin transport under gravity stimulation and auxin transport inhibitor NPA treatment. Furthermore, SA inhibited cell endocytosis of plasma membrane proteins. 【Conclusion】 Salicylic acid could inhibit cell endocytosis to disturb polar auxin transport to regulate rice root development.
引文
[1]康书静,钱前,朱丽.生长素对水稻根系生长发育调控的研究进展[J].中国稻米,2014,20(4):1-8.
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[5]王愿,唐玉梅,王晓坤,等.水杨酸(SA)通过抑制拟南芥中PIN3的表达影响顶端弯钩的形成和下胚轴对向光性的响应[J].植物生理学报,2017(1):89-95.
[6]赵宝海,王世维,郑文静,等.水杨酸诱导水稻抗氧化酶活性及抗瘟性的变化[J].西南农业学报,2015,28(1):127-130.
[7]Li Y,Zhang Z,Nie Y,et al.Proteomic analysis of salicylic acid-induced resistance to Magnaporthe oryzae in susceptible and resistant rice[J].Proteomics,2012,12(14):2340-2354.
[8]Liu X Q,Bai X Q,Qian Q,et al.OsWRKY03,a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1[J].Cell Res,2005,15(8):593-603.
[9]于文娟,钟雪莲,李红松,等.不同水稻品种对南方水稻黑条矮缩病的抗性[J].西南农业学报,2018,31(11):2315-2319.
[10]李培江,张选明,沙志鸿,等.4个稻瘟病基因在籼稻资源中的分布[J].西南农业学报,2018,31(12):2445-2450.
[11]冯德党,吕永刚,王国斌,等.水稻品种生殖生长期耐冷性及其低温胁迫下MADS-box 基因表达差异分析[J].分子植物育种,2012,10(5):501-511.
[12]高东,毛如志,朱有勇.水稻地方品种与改良品种内部遗传异质性的比较分析[J].分子植物育种,2010,8(3):432-438.
[13]翟婉婉,李雪萍,徐返,等.云南水稻地方品种月亮谷的群体多样性分析[J].植物遗传资源学报,2016,17(3):423-432.
[14]董超,徐福荣,杨文毅,等.云南元阳哈尼梯田水稻地方品种月亮谷的遗传变异分析[J].中国水稻科学,2013,27(2):137-144.
[15]包龙丽,何永美,祖艳群,等.大田条件下增强UV-B 辐射对元阳梯田2个地方水稻品种叶片形态解剖结构的影响[J].生态学杂志,2013,32(4):882-889.
[16]赵丹,赵娟,刘涛,等.元阳梯田水稻品种‘月亮谷’根系内生及根际细菌的群落结构[J].云南农业大学学报,2017,32(1):17-26.
[17]Wang J R,Hu H,Wang GH,et al.Expression of PIN genes in rice(Oryza sativa L.):tissue specificity and regulation by hormones[J].Mol.Plant,2009,2(4):823-831.
[18]Chen Y,Fan X,Song W,et al.Over-expression of OsPIN2 leads to increased tiller numbers,angle and shorter plant height through suppression of OsLAZY1[J].Plant Biotechnol J.2012,10(2):139-49.
[19]Kim T H,Kunz H H,Bhattacharjee S,et al.Natural variation in small molecule-induced TIR-NB-LRR signaling induces root growth arrest via EDS1- and PAD4-complexed R protein VICTR in Arabidopsis[J].Plant Cell,2012,24(12):5177-5192.
[20]Zhao X,Wang J,Yuan J,et al.NITRIC OXIDE-ASSOCIATED PROTEIN1(AtNOA1) is essential for salicylic acid-induced root waving in Arabidopsis thaliana[J].New Phytologist,2015,207(1):211-224.
[2]Xu M,Zhu L,Shou H,et al.A PIN1 family gene,OsPIN1,involved in auxin-dependent adventitious root emergence and tillering in rice[J].Plant Cell Physiol,2005,46(10):1674-1681.
[3]Paciorek T,Zazímalová E,Ruthardt N,et al.Auxin inhibits endocytosis and promotes its own efflux from cells[J].Nature,2005,435(7046):1251-1256.
[4]Du Y,Tejos R,Beck M,et al.Salicylic acid interferes with clathrin-mediated endocytic protein trafficking[J].Proc.Natl.Acad.Sci.USA,2013,110(19):7946-7951.
[5]王愿,唐玉梅,王晓坤,等.水杨酸(SA)通过抑制拟南芥中PIN3的表达影响顶端弯钩的形成和下胚轴对向光性的响应[J].植物生理学报,2017(1):89-95.
[6]赵宝海,王世维,郑文静,等.水杨酸诱导水稻抗氧化酶活性及抗瘟性的变化[J].西南农业学报,2015,28(1):127-130.
[7]Li Y,Zhang Z,Nie Y,et al.Proteomic analysis of salicylic acid-induced resistance to Magnaporthe oryzae in susceptible and resistant rice[J].Proteomics,2012,12(14):2340-2354.
[8]Liu X Q,Bai X Q,Qian Q,et al.OsWRKY03,a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1[J].Cell Res,2005,15(8):593-603.
[9]于文娟,钟雪莲,李红松,等.不同水稻品种对南方水稻黑条矮缩病的抗性[J].西南农业学报,2018,31(11):2315-2319.
[10]李培江,张选明,沙志鸿,等.4个稻瘟病基因在籼稻资源中的分布[J].西南农业学报,2018,31(12):2445-2450.
[11]冯德党,吕永刚,王国斌,等.水稻品种生殖生长期耐冷性及其低温胁迫下MADS-box 基因表达差异分析[J].分子植物育种,2012,10(5):501-511.
[12]高东,毛如志,朱有勇.水稻地方品种与改良品种内部遗传异质性的比较分析[J].分子植物育种,2010,8(3):432-438.
[13]翟婉婉,李雪萍,徐返,等.云南水稻地方品种月亮谷的群体多样性分析[J].植物遗传资源学报,2016,17(3):423-432.
[14]董超,徐福荣,杨文毅,等.云南元阳哈尼梯田水稻地方品种月亮谷的遗传变异分析[J].中国水稻科学,2013,27(2):137-144.
[15]包龙丽,何永美,祖艳群,等.大田条件下增强UV-B 辐射对元阳梯田2个地方水稻品种叶片形态解剖结构的影响[J].生态学杂志,2013,32(4):882-889.
[16]赵丹,赵娟,刘涛,等.元阳梯田水稻品种‘月亮谷’根系内生及根际细菌的群落结构[J].云南农业大学学报,2017,32(1):17-26.
[17]Wang J R,Hu H,Wang GH,et al.Expression of PIN genes in rice(Oryza sativa L.):tissue specificity and regulation by hormones[J].Mol.Plant,2009,2(4):823-831.
[18]Chen Y,Fan X,Song W,et al.Over-expression of OsPIN2 leads to increased tiller numbers,angle and shorter plant height through suppression of OsLAZY1[J].Plant Biotechnol J.2012,10(2):139-49.
[19]Kim T H,Kunz H H,Bhattacharjee S,et al.Natural variation in small molecule-induced TIR-NB-LRR signaling induces root growth arrest via EDS1- and PAD4-complexed R protein VICTR in Arabidopsis[J].Plant Cell,2012,24(12):5177-5192.
[20]Zhao X,Wang J,Yuan J,et al.NITRIC OXIDE-ASSOCIATED PROTEIN1(AtNOA1) is essential for salicylic acid-induced root waving in Arabidopsis thaliana[J].New Phytologist,2015,207(1):211-224.