三七总皂苷对过氧化氢所致HUVECS损伤的保护作用
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摘要
目的:探讨三七总皂苷(血塞通)从抗氧化途径对人脐静脉内皮细胞(HUVECS)的保护作用。方法:体外培养HUVECS细胞,采用CCK8法,不同剂量血塞通对正常细胞干预,然后得到25、50、100mg/L为最终剂量。通过不同剂量过氧化氢对于HUVECS的损伤,CCK8法检测细胞活性,得出500μmol/L,3h确定造模条件。按照对照组、模型组、三七总皂苷(血塞通)高、中、低剂量五个组展开实验,对LDH、SOD、CAT、NO、NOS相关氧化指标进行检测。结果:三七总皂苷(血塞通)对正常细胞在0～100mg/L具有促进生长的作用,超过此剂量具有抑制作用;在促进范围剂量内可以降低LDH、MDA表达,升高SOD、CAT、NO、NOS表达。结论论:三七总皂苷(血塞通)对HUVECS氧化而产生的损伤具有保护作用,其机制可能是通过抗氧化途径保护细胞。
Objective: Study PNS(Xuesetong) from the anti-oxidative pathway in human umbilical vein endothelial cells(HUVECS) protection.Methods: HUVECS cells were cultured in vitro, using CCK8 method, different doses PNS(Xuesetong) intervention on normal cells, then get 25, 50,100 mg/L for the final dose. By different doses of hydrogen peroxide for HUVECS damage, CCK8 cell activity assay, draw 500μmol/L, 3 h modeling to determine the conditions. In accordance with the control group, model group, Xuesaitong high, medium and low dose group launched five experiments. Of LDH, SOD, CAT, NO, NOS related oxidative indexes were detected. Results: PNS(Xuesetong) normal cells in the 0～100 mg/L can promote the growth of more than this dose inhibited. In promoting the dose can be reduced in the range of expression of LDH, MDA, elevated SOD,CAT, NO, NOS expression. Conclusions: PNS(XST) injury HUVECS oxidation and has a protective effect, the mechanism is possible to protect cells by anti-oxidative pathway.
Objective: Study PNS(Xuesetong) from the anti-oxidative pathway in human umbilical vein endothelial cells(HUVECS) protection.Methods: HUVECS cells were cultured in vitro, using CCK8 method, different doses PNS(Xuesetong) intervention on normal cells, then get 25, 50,100 mg/L for the final dose. By different doses of hydrogen peroxide for HUVECS damage, CCK8 cell activity assay, draw 500μmol/L, 3 h modeling to determine the conditions. In accordance with the control group, model group, Xuesaitong high, medium and low dose group launched five experiments. Of LDH, SOD, CAT, NO, NOS related oxidative indexes were detected. Results: PNS(Xuesetong) normal cells in the 0～100 mg/L can promote the growth of more than this dose inhibited. In promoting the dose can be reduced in the range of expression of LDH, MDA, elevated SOD,CAT, NO, NOS expression. Conclusions: PNS(XST) injury HUVECS oxidation and has a protective effect, the mechanism is possible to protect cells by anti-oxidative pathway.
引文
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[2]谢辉,陈俊.三七总皂苷对心肌细胞肥大及凋亡的影响[J].山西医药杂志,2010,39(11):1059-1560.
[3]张福康,杨妍华,陆再华.三七总皂苷对以尿酸钠诱导血管内皮细胞凋亡的影响[J].中华中医药学刊,2012,(8):1843-1845.
[4]马桂鑫,赵文文,陈修平.血管内皮细胞损伤模型及中药保护作用研究进展[J].中草药,2014,45(2):276-283.
[5]李钊飞,曹小雨,陈琼芳,等.灯盏花素对过氧化氢致人脐静脉内皮细胞损伤的保护作用[J].时珍国医国药,2016,27(7):1566-1569.
[6]Koepke JI,Nakrieko KA,Wood CS,et al.Restoration of peroxisomal catalase import in a model of human cellular aging[J].Traffic,2007,8(11):1590-1600.
[7]Migliore L,CoppedèF.Environmental-induced oxidative stress in neurodegenerative disorders and aging[J].Mutat Res,2009,674(1-2):73-84.
[8]Storz G,Imlay JA.Oxidative Stress[J].Curr Opin Microbiol,1999,2(2):188-194.
[9]甘雨,徐惠波,孙晓波.三七总皂苷的药理作用研究进展[J].时珍国医国药,2007,18(5):1251-1252.
[10]刘晓丹,邓常清.黄芪甲苷和三七总皂苷中主要有效成分抗PC12细胞氧化损伤的配伍研究[J].湖南中医药大学学报,2012,32(1):8-12.
[11]蔡鹰,魏群利,陆晓和.回心草对脐静脉内皮细胞的保护作用及对分泌一氧化氮和一氧化氮合酶的影响[J].中国实验方剂学杂志,2009,15(7):79-82.