五味子中山葡萄HPLC指纹图谱的建立及其伪品鉴别
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摘要
目的建立五味子Schisandra chmensis (Turcz.) Bail中山葡萄HPLC指纹图谱及其伪品鉴别。方法五味子水提物的分析采用Waters symmetry shieldTMRP18柱(4. 6 mm×250 mm,5. 0μm);流动相乙腈-水,梯度洗脱;柱温30℃;体积流量1. 0 m L/min;检测波长210 nm。结果 10批样品指纹图谱中有10个共有峰,相似度均大于0. 90。用该液相方法检测山葡萄与五味子,指纹图谱有显著差异。结论该方法准确灵敏、重复性好,能够准确鉴别五味子伪品山葡萄。
AIM To establish the HPLC fingerprints of Vitis amurensis from Schisandra chinensis( Turcz.)Bail and to identify its counterfeit. METHODS The analysis of aqueous extract from S. chinensis was developed on a 30 ℃ thermostatic Waters symmetry shieldTMRP18column( 4. 6 mm× 250 mm,5. 0 μm),with the mobile phase comprising of acetonitrile-water flowing at 1. 0 m L/min in a gradient elution manner,and the detection wavelength was set at 210 nm. RESULTS There were ten common peaks in the fingerprints of ten batches of samples,with the similarities of more than 0. 90. There were significant differences in the HPLC fingerprints between V.amurensis and S. chinensis. CONCLUSION This accurate,stable and reproducible method can be used to identify the V. amurensis from S. chinensis.
AIM To establish the HPLC fingerprints of Vitis amurensis from Schisandra chinensis( Turcz.)Bail and to identify its counterfeit. METHODS The analysis of aqueous extract from S. chinensis was developed on a 30 ℃ thermostatic Waters symmetry shieldTMRP18column( 4. 6 mm× 250 mm,5. 0 μm),with the mobile phase comprising of acetonitrile-water flowing at 1. 0 m L/min in a gradient elution manner,and the detection wavelength was set at 210 nm. RESULTS There were ten common peaks in the fingerprints of ten batches of samples,with the similarities of more than 0. 90. There were significant differences in the HPLC fingerprints between V.amurensis and S. chinensis. CONCLUSION This accurate,stable and reproducible method can be used to identify the V. amurensis from S. chinensis.
引文
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[14]国家药品监督管理局.中药注射剂指纹图谱研究的技术要求(暂行)[S].中国药品标准,2000,1(4):3-7.
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[2]周永峰,王伽伯,张定堃,等.不同产地五味子药材的质量特征比较研究[J].中国中药杂志,2015,40(16):3152-3157.
[3]范文成,常美玲,张国良,等.不同产地野生五味子的含量研究[J].中国药房,2011,22(3):248-250.
[4]韩志福,胡高升,李娜,等.不同产地五味子质量评价及抗氧化活性研究[J].中药材, 2012, 35(12):1904-1909.
[5]胡双丰,杨志芬.北五味子与南五味子的鉴别[J].中医学报,2010,25(6):1149-1150.
[6]谭春梅,黄琴伟,张文婷,等.五味子和南五味子的HPLC指纹图谱研究[J].中国现代应用药学,2008,25(6):514-517.
[7]刘少静,李艳,张颖,等. RP-HPLC测定南、北五味子中三种木脂素类成分的含量[J].应用化工,2014,43(11):2107-2110.
[8]邓力,王浩兵,严书超,等.超高效液相色谱法同时测定南、北五味子中的7种木脂素含量[J].中国医院药学杂志,2017,37(12):1158-1162.
[9]李静,胡晓炜,宋旭峰.南五味子伪品火棘果的鉴别[J].中草药,2003,34(8):附1.
[10]方洪征,张庆玲.一种五味子伪品的真假鉴别[J].基层中药杂志,2002,16(6):33-33.
[11]杜秀芬,廉景魁,翟铁宏,等.五味子伪品鸡树条果的鉴别[J].中药材,1994,17(9):23-24.
[12]汪平,邬芙蓉,成龙.五味子中掺伪品南五味子的鉴别[J].中国药业,2004,13(2):62-63.
[13]熊云珍,肖凌,但汉雄.南五味子伪品山葡萄的鉴别研究[J].湖北中医药大学学报,2012,14(5):42-43.
[14]国家药品监督管理局.中药注射剂指纹图谱研究的技术要求(暂行)[S].中国药品标准,2000,1(4):3-7.
[15]国家食品药品监督管理局.生脉注射液国家标准[S].