摘要
目的建立五味子Schisandra chmensis (Turcz.) Bail中山葡萄HPLC指纹图谱及其伪品鉴别。方法五味子水提物的分析采用Waters symmetry shieldTMRP18柱(4. 6 mm×250 mm,5. 0μm);流动相乙腈-水,梯度洗脱;柱温30℃;体积流量1. 0 m L/min;检测波长210 nm。结果 10批样品指纹图谱中有10个共有峰,相似度均大于0. 90。用该液相方法检测山葡萄与五味子,指纹图谱有显著差异。结论该方法准确灵敏、重复性好,能够准确鉴别五味子伪品山葡萄。
AIM To establish the HPLC fingerprints of Vitis amurensis from Schisandra chinensis( Turcz.)Bail and to identify its counterfeit. METHODS The analysis of aqueous extract from S. chinensis was developed on a 30 ℃ thermostatic Waters symmetry shieldTMRP18column( 4. 6 mm× 250 mm,5. 0 μm),with the mobile phase comprising of acetonitrile-water flowing at 1. 0 m L/min in a gradient elution manner,and the detection wavelength was set at 210 nm. RESULTS There were ten common peaks in the fingerprints of ten batches of samples,with the similarities of more than 0. 90. There were significant differences in the HPLC fingerprints between V.amurensis and S. chinensis. CONCLUSION This accurate,stable and reproducible method can be used to identify the V. amurensis from S. chinensis.
引文
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