毛细管电泳法同时分析水、乳、膏类化妆品中5种有机酸
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摘要
建立了毛细管电泳同时分析5种有机酸的间接紫外检测法。优化了背景电解质溶液中表面活性剂十四烷基三甲基溴化铵(TTAB)的浓度和溶液pH。优化后的电泳分析条件如下:含0.4 mmol/L TTAB的15 mmol/L邻苯二甲酸氢钾溶液为背景电解质(pH 5.6);分离电压-15 kV;检测波长254 nm;分离温度25℃;进样压力5 kPa;进样时间5 s。在此条件下,可在6 min内完成5种有机酸的同时分离检测,线性范围为甲酸15～600 mg/L、苹果酸30～800 mg/L、柠檬酸20～700 mg/L、乙醇酸40～500 mg/L和乳酸30～5 000 mg/L,线性相关系数为0.998 3～0.999 8;检出限为0.1～2.0μg/g。该方法可用于检测水状、乳状、膏状3类化妆品中的5种有机酸。在3个加标水平下,有机酸分析的回收率为95.0%～101.6%, RSD在2.0%以内。该方法操作简单,分析快速,安全环保,灵敏度高,重现性好,有望用于化妆品生产和保存过程中有机酸的监测。
An indirect ultraviolet detection method for simultaneous analysis of five organic acids by capillary electrophoresis was established. The concentration of the tetradecyltrimethylammonium bromide(TTAB) surfactant in the background electrolyte solution and the pH of the solution were optimized. Five organic acids—formic acid, malic acid, citric acid, glycolic acid, lactic acid—were effectively separated from each other within 6 min with an electrolyte solution(15 mmol/L potassium hydrogen phthalate and 0.4 mmol/L TTAB, pH 5.6) under conditions of-15 kV, 25 ℃, and 254 nm, while the injection pressure was 5 kPa and the injection time was 5 s. The linear ranges were 15-600 mg/L, 30-800 mg/L, 20-700 mg/L, 40-500 mg/L, and 30-5 000 mg/Lfor formic acid, malic acid, citric acid, glycolic acid, and lactic acid, respectively, with correlation coefficients of 0.998 3-0.999 8. The detection limits were between 0.1 μg/g and 2.0 μg/g. The method can be used for the detection of five organic acids in three types of cosmetics including aqueous, lotion and cream. At three spiked levels, the recoveries of organic acids were between 95.0% and 101.6%, and relative standard deviations(RSDs) were within 2.0%. The proposed method is easy, rapid, environment friendly, and sensitive with good reproducibility. It can be used for the monitoring of five organic acids in the production and storage of cosmetics including aqueous, lotion and cream.
An indirect ultraviolet detection method for simultaneous analysis of five organic acids by capillary electrophoresis was established. The concentration of the tetradecyltrimethylammonium bromide(TTAB) surfactant in the background electrolyte solution and the pH of the solution were optimized. Five organic acids—formic acid, malic acid, citric acid, glycolic acid, lactic acid—were effectively separated from each other within 6 min with an electrolyte solution(15 mmol/L potassium hydrogen phthalate and 0.4 mmol/L TTAB, pH 5.6) under conditions of-15 kV, 25 ℃, and 254 nm, while the injection pressure was 5 kPa and the injection time was 5 s. The linear ranges were 15-600 mg/L, 30-800 mg/L, 20-700 mg/L, 40-500 mg/L, and 30-5 000 mg/Lfor formic acid, malic acid, citric acid, glycolic acid, and lactic acid, respectively, with correlation coefficients of 0.998 3-0.999 8. The detection limits were between 0.1 μg/g and 2.0 μg/g. The method can be used for the detection of five organic acids in three types of cosmetics including aqueous, lotion and cream. At three spiked levels, the recoveries of organic acids were between 95.0% and 101.6%, and relative standard deviations(RSDs) were within 2.0%. The proposed method is easy, rapid, environment friendly, and sensitive with good reproducibility. It can be used for the monitoring of five organic acids in the production and storage of cosmetics including aqueous, lotion and cream.
引文
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[8] Zhang H,Wang B,Zhou S W.Shanghai Journal of Preventive Medicine,2002,14(6):261 张泓,王斌,周世伟.上海预防医学杂志,2002,14(6):261
[9] Wang Z X,Chen Y,Guo Q.Chinese Chem Lett,2000,11(9):803
[10] Wang H,Gu J L G,Fu R N,et al.Journal of Beijing Institute of Technology (English Version),1998,7(1):43
[11] Wang X Q,Zhao X Y,Liu P D,et al.Chinese Journal of Chromatography,2016,34(2):121 王晓倩,赵新颖,刘品多,等.色谱,2016,34(2):121
[12] Sun B,Mu X,Qi L.Anal Chim Acta,2014,821:97
[13] Han H F,Wang Q,Liu X,et al.Chinese Journal of Chromatography,2012,30(5):538 韩海峰,王庆,刘霞,等.色谱,2012,30(5):538
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[15] Gatea F,Teodor E D,Paun G,et al.Food Anal Methods,2015,8:1335
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[17] Liu Q,Wang L,Hu J,et al.Food Anal Methods,2017,10:111
[18] Costa E V,Lima D L D,Evtyugin D V,et al.Wood Sci Technol,2014,48(1):99
[19] Wang X L,Yu Z Q,Zhu L,et al.Chemistry & Bioengineering,2017,34(9):64 汪雪莲,於钊庆,朱莉,等.化学与生物工程,2017,34(9):64
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[22] Kitagawa F,Otsuka K.J Chromatogr A,2014,1335:42
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[24] Dutra E A,Santoro M I R M,Micke G A,et al.J Pharm Biomed Anal,2006,40(2):242
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[27] Xing L H,Li W L,Qu H B.Chinese Journal of Pharmaceutical Analysis,2011,31(3):547 邢丽红,李文龙,瞿海斌.药物分析杂志,2011,31(3):547
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[32] Chen Z,Naidu R.J Chromatogr A,2004,1023(1):151
[33] Rivasseau C,Boisson A M,Mongélard G,et al.J Chromatogr A,2006,1129(2):283
[34] He J,Chen S,Yu Z.J Chromatogr A,2002,973(1/2):197
[35] Naidu R,Chen Z L.Chromatographia,2001,54(7/8):495
[36] Chen Z,Naidu R.Int J Environ Anal Chem,2003,83(9):749