壮药扁担藤HPLC指纹图谱研究
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摘要
建立壮药扁担藤的HPLC指纹图谱。采用高效液相色谱法,用Ultimate C_(18)(5μm,4.6×250mm)色谱柱对10批壮药扁担藤提取液进行分离分析,以乙腈-0.1%醋酸溶液(体积分数)为流动相,梯度洗脱,检测波长为258nm,柱温为30℃,流速为1.0mL/min,采用"中药色谱指纹图谱相似度评价系统"(2004A)软件进行分析。结果表明,10批壮药扁担藤的HPLC指纹图谱共标定12个共有指纹峰,相似度基本达到0.9以上,为建立壮药扁担藤质量控制方法提供了科学依据。
To study on the HPLC fingerprint of stem of Tetrastigma planicaule(Hook.f.)Gagnep.high performance of liquid chromatography(HPLC)was adopted,the separation was performed on Ultimate C_(18)(5μm,4.6×250 mm)column,the mobile phase was acetonitrile-0.1% acetic acid solution with a gradient elution,the detection wavelength was 258 nm,the column temperature was set at 30℃and the flow rate was 1mL/min.The data was performed with similarity evaluation system for chromatographic fingerprint of TCM(Version 2004A).In the fingerprint,12 common peaks were confirmed and the similarity of 10 batches of stem of Tetrastigma planicaule(Hook.f.)Gagnep.were above 0.9.The experiment result could provide scientific foundation for building the quality control method of Tetrastigma planicaule(Hook.f.)Gagnep.
To study on the HPLC fingerprint of stem of Tetrastigma planicaule(Hook.f.)Gagnep.high performance of liquid chromatography(HPLC)was adopted,the separation was performed on Ultimate C_(18)(5μm,4.6×250 mm)column,the mobile phase was acetonitrile-0.1% acetic acid solution with a gradient elution,the detection wavelength was 258 nm,the column temperature was set at 30℃and the flow rate was 1mL/min.The data was performed with similarity evaluation system for chromatographic fingerprint of TCM(Version 2004A).In the fingerprint,12 common peaks were confirmed and the similarity of 10 batches of stem of Tetrastigma planicaule(Hook.f.)Gagnep.were above 0.9.The experiment result could provide scientific foundation for building the quality control method of Tetrastigma planicaule(Hook.f.)Gagnep.
引文
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[13]刘焕蓉,郭志强,秦雪梅.山西道地药材黄芪石油醚提取物指纹图谱研究[J].广西师范大学学报(自然科学版),2003,21(3):200-201.
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[15]胡坤,赵书林,李舒婷,等.罗汉果的毛细管电泳指纹图谱研究[J].广西师范大学学报(自然科学版),2008,26(2):84-87.
[2]周亮,黄建平,黄自云.热带雨林老茎开花:扁担藤[J].园林,2012,1:70-71.
[3]台海川,徐荔,王娟,等.傣药扁担藤质量标准研究[J].中国民族医药杂志,2012,11:45-46.
[4]覃迅云,罗金裕,高志刚.中国瑶药学[M].北京:民族出版社,2002:141.
[5]江苏新医学院.中药大辞典[M].上海:上海人民出版社,1986:1741.
[6]甄汉深,梁晓乐,丘琴,等.壮药扁担藤的性状及显微鉴别[J].中药材,2013,36(6):900.
[7]潘乔丹,熊圆圆,陈文东,等.扁担藤总黄酮和总三萜提取工艺及抗氧化研究[J].中药材,2012,35(10):1679-1683.
[8]潘乔丹,熊圆圆,陈文东,等.扁担藤不同极性成分抗氧化活性研究[J].中国实验方剂学杂志,2013,19(1):232-235.
[9]李兵,廖广凤,黄业玲,等.瑶药扁担藤乙酸乙酯部位化学成分研究[J].中药材,2014,37(4):610-611.
[10]卢澄生,李兵.瑶药扁担藤的化学成分预试验研究[J].广西中医学院学报,2011,14:43-44.
[11]邵加春,何翠红,雷婷,等.瑶药扁担藤化学成分的研究[J].中国药学杂志,2010,21(45):1615-1617.
[12]曾立,罗辉,李庚喜,等.瑶药化学成分及其资源开发[J].中国野生植物资源,2011,30(5):23-25.
[13]刘焕蓉,郭志强,秦雪梅.山西道地药材黄芪石油醚提取物指纹图谱研究[J].广西师范大学学报(自然科学版),2003,21(3):200-201.
[14]丘琴,甄汉深,陈明伟,等.壮药龙利叶茎HPLC指纹图谱研究[J].广西师范大学学报(自然科学版),2016,34(2):116-121.
[15]胡坤,赵书林,李舒婷,等.罗汉果的毛细管电泳指纹图谱研究[J].广西师范大学学报(自然科学版),2008,26(2):84-87.