凝胶净化结合高效液相色谱-蒸发光散射检测器测定食用油脂中胆固醇含量
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摘要
建立了凝胶净化系统与高效液相色谱-蒸发光散射检测器结合对食用油脂中胆固醇含量测定的方法。结果表明:胆固醇质量浓度在2~200μg/m L范围内,胆固醇质量浓度的对数与峰面积对数线性关系良好,相关系数为0.998 9,检出限为10 mg/kg,回收率为94.01%~103.80%,相对标准偏差为1.12%~7.25%。该方法可有效减少烦琐的前处理过程对样品中胆固醇含量造成的损失,避免检测过程中出现溶剂响应和溶剂峰干扰,方法简单、可行、有效。
A method for the determination of cholesterol content in edible oils and fats was established by gel permeation chromatography system combined with liquid chromatography-evaporative light scattering detector. The results showed that the linearity correlation was good in the range of 2-200 μg/m L with correlation efficient 0. 998 9. The limit of detection,recovery rate and relative standard deviation were10 mg/kg,94. 01%-103. 80% and 1. 12%-7. 25% respectively. The method could effectively reduce the loss of cholesterol caused by cumbersome pre-treatment process,and avoid solvent response and solvent peak interference during detection. The method was proved to be simple,feasible and effective.
A method for the determination of cholesterol content in edible oils and fats was established by gel permeation chromatography system combined with liquid chromatography-evaporative light scattering detector. The results showed that the linearity correlation was good in the range of 2-200 μg/m L with correlation efficient 0. 998 9. The limit of detection,recovery rate and relative standard deviation were10 mg/kg,94. 01%-103. 80% and 1. 12%-7. 25% respectively. The method could effectively reduce the loss of cholesterol caused by cumbersome pre-treatment process,and avoid solvent response and solvent peak interference during detection. The method was proved to be simple,feasible and effective.
引文
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[3]丁卓平,王明化,刘振华,等.食品中胆固醇含量测定方法的研究与比较[J].食品科学,2004,25(1):130-135.
[4]李黎,刘玉峰,唐华澄,等.高效液相色谱法测定食品中的胆固醇[J].食品科学,2004,28(4):289-291.
[5]李珂,高允生,李炳龙,等.高效液相色谱-蒸发光散射检测器法检测天然牛黄与酶促牛黄中胆固醇含量[J].医药导报,2011,30(12):1633-1636.
[6]张旭,胡艳,蒋桂韬,等.蛋黄中胆固醇含量测定方法的研究[J].检测分析,2011(12):35-38.
[7]高瑜莹,裘爱泳,潘秋琴,等.植物甾醇的分析方法[J].中国油脂,2001,26(1):25-28.
[8]张庆波.气相色谱质谱法检测食品中胆固醇含量的研究[D].长春:吉林大学,2013.
[9]赵丽娟,彭跃,关屏.凝胶色谱在有机前处理中的应用[C].辽宁省环境科学学会学术年会,2006.
[10]魏作君,钱国平,王慧,等.有机溶剂中胆固醇溶解性能研究[C].全国化学工程与生物化工年会,2005.
[11]周相娟,李伟,许华,等.凝胶渗透色谱技术及其在食品安全检测方面的应用[J].现代仪器,2009(1):1-4.
[12]刘苇,金成,孔维军,等.高效液相色谱蒸发光散射法测定牛黄中多种有效成分含量及其质量相关性研究[J].分析化学,2010,38(4):522-526.
[13]曹雨震,周倩如.蒸发光散射检测器及其应用[J].现代商检科技,1995,5(6):46-51.
[14]王巧娥,丁明玉.蒸发光散射检测技术研究进展[J].分析测试学报,2006,25(6):126-132.
[15]张蓉真,李珑,刘树滔,等.测定鸡蛋胆固醇的高效液相色谱新方法[J].色谱,1998,16(2):91-94.