互叶白千层油(茶树油)对肉鸡小肠黏膜组织结构及肠道免疫细胞的影响
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摘要
选用1日龄的雌性岭南黄肉鸡99只,随机分为3组,分别为对照组、试验Ⅰ组和试验Ⅱ组,每组33只。对照组常规饲养,试验Ⅰ组和Ⅱ组分别在饮水中添加0.012 mL/L和0.020 mL/L的茶树油,试验至45日龄结束。解剖取十二指肠、空肠、回肠进行HE染色和PAS染色,观察小肠黏膜形态、上皮内淋巴细胞及杯状细胞数目。结果显示,2个试验组与对照组相比,各段小肠的绒毛长度增加,肠壁变薄,隐窝深度变浅,肠绒毛高度/隐窝深度比值增加;2个试验组的上皮内杯状细胞和淋巴细胞的数目高于对照组。结果表明,茶树油能够改善肉鸡小肠黏膜结构,增加小肠上皮内免疫细胞数量,提示茶树油对肉鸡的肠道吸收和肠道免疫功能具有增强作用。
This experiment was designed to observe the effects of different concentrations of Melaleuca alternariae oil(tea tree oil,TTO) on the intestinal mucosal structure and intestinal immune cells in broilers.99 one day old female Lingnan yellow chickens were randomly divided into three groups:the control group,the test Ⅰ group and the test Ⅱ group,with 33 birds in each group.The control group was fed conventionally,while test Ⅰ and test Ⅱ groups were fed with 0.012 mL/L and 0.020 mL/L tea tree oil in drinking water.The experiment ended on day 45.The duodenum,jejunum and ileum were dissected and HE staining and PAS staining were performed to observe the morphology of the intestinal mucosa,the number of intraepithelial lymphocytes and goblet cells.The results showed that broilers of two experimental groups increased villus height,thinner wall of the intestine,shallower depth of crypts of each small intestine compared with control group.The ratio of intestinal villi height/crypt depth,the number of goblet cells and lymphocytes in the two experimental groups was higher than those of the control group.It is concluded that tea tree oil could improve the intestinal mucosal structure of broilers,and increase the number of the intestinal immune cells,suggesting that tea tree oil has the function of promoting intestinal absorption and intestinal immune function of broilers.
This experiment was designed to observe the effects of different concentrations of Melaleuca alternariae oil(tea tree oil,TTO) on the intestinal mucosal structure and intestinal immune cells in broilers.99 one day old female Lingnan yellow chickens were randomly divided into three groups:the control group,the test Ⅰ group and the test Ⅱ group,with 33 birds in each group.The control group was fed conventionally,while test Ⅰ and test Ⅱ groups were fed with 0.012 mL/L and 0.020 mL/L tea tree oil in drinking water.The experiment ended on day 45.The duodenum,jejunum and ileum were dissected and HE staining and PAS staining were performed to observe the morphology of the intestinal mucosa,the number of intraepithelial lymphocytes and goblet cells.The results showed that broilers of two experimental groups increased villus height,thinner wall of the intestine,shallower depth of crypts of each small intestine compared with control group.The ratio of intestinal villi height/crypt depth,the number of goblet cells and lymphocytes in the two experimental groups was higher than those of the control group.It is concluded that tea tree oil could improve the intestinal mucosal structure of broilers,and increase the number of the intestinal immune cells,suggesting that tea tree oil has the function of promoting intestinal absorption and intestinal immune function of broilers.
引文
[1] 李锦钰.植物提取物替代抗生素作饲料添加剂的论证[J].北方牧业,2014(7):44.
[2] PAZYAR N,YAGHOOBI R,BAGHERANI N,et al.A review of applications of tea tree oil in dermatology[J].Int J Dermatol,2013,52(7):784-790.
[3] SHI C,ZHAO X,YAN H,et al.Effect of tea tree oil on Staphylococcus aureus growth and enterotoxin production[J].Food Control,2016,62:257-263.
[4] NOGUEIR A M N M,AQUINO S G,JUNIOR C R,et al.Terpinen-4-ol and alpha-terpineol (tea tree oil components) inhibit the production of IL-1β,IL-6 and IL-10 on human macrophages[J].Inflamm Res,2014,63(9):769-778..
[5] 樊丽妃.引种互叶白千层茶树油的抑菌活性与抗氧化性研究[D].广东广州:华南理工大学,2011.
[6] LI X,DUAN S,CHU C,et al.Melaleuca alternifolia concentrate inhibits in vitro entry of influenza virus into host cells[J].Molecules,2013,18(8):9550-9566.
[7] 封飞飞,方伟,王淑楠,等.茶树油对育肥猪生长性能、器官指数、胴体性状和肉品质的影响[J].动物营养学报,2017,29(10):3620-3626.
[8] 王淑楠,董丽,魏臻武,等.茶树油对断奶仔猪生长性能、血清生化指标及脏器指数的影响[J].中国畜牧兽医,2018,45(1):86-92.
[9] 郝丹丹,张旭,陈嘉,等.牛至油对成年蛋鸡生长性能和免疫功能的影响[J].中国兽医学报,2017,37(6):1121-1127.
[10] AWAD W A,GHAREEB K,ABDEL-RAHEEM S,et al.Effects of dietary inclusion of probiotic and synbiotic on growth performance,organ weights,and intestinal histomorphology of broiler chickens[J].Poult Sci,2009,88(1):49-56.
[11] 刘晓静,史彬林,赵育国,等.日粮中添加沙蒿籽粉对肉仔鸡肠绒毛形态及肠道菌群的影响[J].饲料工业,2011(5):13-15.
[12] 石琳,张少成,陈广信,等.不同营养水平饲粮添加酵母浓缩物对罗斯肉鸡生长性能、肠道消化酶活性及肠黏膜组织结构的影响[J].中国兽医学报,2018,38(1):201-205.
[13] 燕磊,朱正鹏,吕尊周,等.饲粮中添加不同植物精油对肉仔鸡生长性能、肠道发育、免疫器官指数及屠宰性能的影响[J].动物营养学报,2017(4):284-292.
[14] KIM J S,INGALE S L,KIM Y W,et al.Effect of supplementation of multi-microbe probiotic product on growth performance,apparent digestibility,cecal microbiota and small intestinal morphology of broilers[J].J Anim Physiol Anim Nutr,2012,96(4):618-626.
[15] SHI Q,GAO G,XING C,et al.Effects of traditional Chinese medicine on numbers of lymphocytes and goblet cells in villus epithelia of layers under heat stress[J].Agric Sci Technol,2015,16(2):311.
[16] KIM Y S,HO S B.Intestinal goblet cells and mucins in health and disease:recent insights and progress[J].Curr Gastroenterol Rep,2010,12(5):319-330.
[17] 邢亚丽,宋慧,姜云瑶,等.树舌发酵浸膏多糖对肉鸡肠黏膜组织结构及肠道免疫细胞的影响[J].中国农业大学学报,2015,20(3):126-132.
[2] PAZYAR N,YAGHOOBI R,BAGHERANI N,et al.A review of applications of tea tree oil in dermatology[J].Int J Dermatol,2013,52(7):784-790.
[3] SHI C,ZHAO X,YAN H,et al.Effect of tea tree oil on Staphylococcus aureus growth and enterotoxin production[J].Food Control,2016,62:257-263.
[4] NOGUEIR A M N M,AQUINO S G,JUNIOR C R,et al.Terpinen-4-ol and alpha-terpineol (tea tree oil components) inhibit the production of IL-1β,IL-6 and IL-10 on human macrophages[J].Inflamm Res,2014,63(9):769-778..
[5] 樊丽妃.引种互叶白千层茶树油的抑菌活性与抗氧化性研究[D].广东广州:华南理工大学,2011.
[6] LI X,DUAN S,CHU C,et al.Melaleuca alternifolia concentrate inhibits in vitro entry of influenza virus into host cells[J].Molecules,2013,18(8):9550-9566.
[7] 封飞飞,方伟,王淑楠,等.茶树油对育肥猪生长性能、器官指数、胴体性状和肉品质的影响[J].动物营养学报,2017,29(10):3620-3626.
[8] 王淑楠,董丽,魏臻武,等.茶树油对断奶仔猪生长性能、血清生化指标及脏器指数的影响[J].中国畜牧兽医,2018,45(1):86-92.
[9] 郝丹丹,张旭,陈嘉,等.牛至油对成年蛋鸡生长性能和免疫功能的影响[J].中国兽医学报,2017,37(6):1121-1127.
[10] AWAD W A,GHAREEB K,ABDEL-RAHEEM S,et al.Effects of dietary inclusion of probiotic and synbiotic on growth performance,organ weights,and intestinal histomorphology of broiler chickens[J].Poult Sci,2009,88(1):49-56.
[11] 刘晓静,史彬林,赵育国,等.日粮中添加沙蒿籽粉对肉仔鸡肠绒毛形态及肠道菌群的影响[J].饲料工业,2011(5):13-15.
[12] 石琳,张少成,陈广信,等.不同营养水平饲粮添加酵母浓缩物对罗斯肉鸡生长性能、肠道消化酶活性及肠黏膜组织结构的影响[J].中国兽医学报,2018,38(1):201-205.
[13] 燕磊,朱正鹏,吕尊周,等.饲粮中添加不同植物精油对肉仔鸡生长性能、肠道发育、免疫器官指数及屠宰性能的影响[J].动物营养学报,2017(4):284-292.
[14] KIM J S,INGALE S L,KIM Y W,et al.Effect of supplementation of multi-microbe probiotic product on growth performance,apparent digestibility,cecal microbiota and small intestinal morphology of broilers[J].J Anim Physiol Anim Nutr,2012,96(4):618-626.
[15] SHI Q,GAO G,XING C,et al.Effects of traditional Chinese medicine on numbers of lymphocytes and goblet cells in villus epithelia of layers under heat stress[J].Agric Sci Technol,2015,16(2):311.
[16] KIM Y S,HO S B.Intestinal goblet cells and mucins in health and disease:recent insights and progress[J].Curr Gastroenterol Rep,2010,12(5):319-330.
[17] 邢亚丽,宋慧,姜云瑶,等.树舌发酵浸膏多糖对肉鸡肠黏膜组织结构及肠道免疫细胞的影响[J].中国农业大学学报,2015,20(3):126-132.