聚丙烯酰胺降解组合菌的筛选和降解条件优化
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摘要
从大庆油田含聚污水中分离得到3株聚丙烯酰胺降解菌(N2-2-P139-D1、N2-2-P140-D1和N2-2-P140-X1),进行生理生化检测和16SrDNA分子生物学鉴定,在不同pH条件下混合培养,研究3株聚丙烯酰胺降解菌的最优降解组合、降解pH和降解时间。结果表明:3株聚丙烯酰胺降解菌分别属于纤维微菌属、不动杆菌属和假单胞菌属。在pH为7.0时,N2-2-P139-D1、N2-2-P140-D1和N2-2-P140-X1组合的降解率最大,最优降解时间为7d时,降解率为39.5%;在pH为9.0时,N2-2-P139-D1和N2-2-P140-X1组合的降解率最大,最优降解时间为5d时,降解率为34.5%。复合菌N2-2-P139-D1和N2-2-P140-X1,以及复合菌N2-2-P139-D1、N2-2-P140-D1和N2-2-P140-X1对现场含聚污水中聚丙烯酰胺的最大降解率分别为35.4%和31.0%,最优降解时间为5d。该结果为微生物在大庆油田采出液油水分离和含聚污水处理提供支持。
Abstrcat:In this study,three HPAM-degrading bacterial strains named by N2-2-P139-D1,N2-2-P140-D1 and N2-2-P140-X1 were isolated from polymer-containing wastewater in Daqing Oilfield.The results of physiological and biochemical tests and analysis of 16 SrDNA sequence showed that these strains belonged to Cellulosimicrobium sp.,Acinetobacter sp.and Pseudomonas sp.respectively.In order to study the degradation combination,pH value and degradation time of Polyacrylamide in polymer-bearingwastewater,the bacteria were mixed in different pH environments.The results showed that:the optimal combination was N2-2-P139-D1,N2-2-P140-D1 and N2-2-P140-X1,the degradation rate was 39.5%in pH 7.0,and the optimal time for degradation was 7 days;the optimal combination of N2-2-P139-D1 and N2-2-P140-X1,the degradation rate was 34.5%in pH 9.0,and the optimal time for degradation was 5 days.The polyacrylamide degradation rate of polymeric wastewater was 35.4% and 31.0% respectively by the combination of N2-2-P139-D1 and N2-2-P140-X1,and the combination N2-2-P139-D1,N2-2-P140-D1 and N2-2-P140-X1,and the optimal degradation time was 5 days.The results can provide some technical support for the application of microorganisms in oil-water separation and polymer-bearing wastewater treatment in Daqing Oilfield.
Abstrcat:In this study,three HPAM-degrading bacterial strains named by N2-2-P139-D1,N2-2-P140-D1 and N2-2-P140-X1 were isolated from polymer-containing wastewater in Daqing Oilfield.The results of physiological and biochemical tests and analysis of 16 SrDNA sequence showed that these strains belonged to Cellulosimicrobium sp.,Acinetobacter sp.and Pseudomonas sp.respectively.In order to study the degradation combination,pH value and degradation time of Polyacrylamide in polymer-bearingwastewater,the bacteria were mixed in different pH environments.The results showed that:the optimal combination was N2-2-P139-D1,N2-2-P140-D1 and N2-2-P140-X1,the degradation rate was 39.5%in pH 7.0,and the optimal time for degradation was 7 days;the optimal combination of N2-2-P139-D1 and N2-2-P140-X1,the degradation rate was 34.5%in pH 9.0,and the optimal time for degradation was 5 days.The polyacrylamide degradation rate of polymeric wastewater was 35.4% and 31.0% respectively by the combination of N2-2-P139-D1 and N2-2-P140-X1,and the combination N2-2-P139-D1,N2-2-P140-D1 and N2-2-P140-X1,and the optimal degradation time was 5 days.The results can provide some technical support for the application of microorganisms in oil-water separation and polymer-bearing wastewater treatment in Daqing Oilfield.
引文
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[18]李殿杰.聚丙烯酰胺降解菌株的分离及其油田含聚污水生物强化处理研究[J].环境科学与管理,2018,43(1):113-117.LI Dianjie.Isolation of HPAM degrading bacteria strain and polymer containing oilfield wastewater treatment[J].Environmental Science and Management,2018,43(1):113-117.
[19]陈庆国.聚丙烯酰胺降解菌的筛选及降解含聚丙烯酰胺污水的室内研究[D].青岛:中国海洋大学,2009:35-55.CHEN Qingguo.Screening and valuing of HPAM-degrading bacteria and studies on biochemical treatment of wastewater containing HAPM[D].Qingdao:Ocean University of China,2009:35-55.
[20]李淑芹,孙靖,韦微,等.三株聚丙烯酰胺降解菌联合降解特性研究[J].东北农业大学学报,2015,46(8):21-25.LI Shuqin,SUN Jing,WEI Wei,et al.Study on the characteristics of combination degradation by three PAM degrading-bacteria[J].Journal of Northeast Agricultural University,2015,46(8):21-25.
[2]陈玉丽.聚驱后微生物调驱菌种室内优选实验研究[J].精细石油化工进展,2012,13(1):1-3.CHEN Yuli.Laboratory optimization of microbial strains for MEOR after polymer flooding[J].Advances in Fine Petrochemicals,2012,13(1):1-3.
[3]叶清.三元复合驱采出水特性分析及其处理方法研究[D].上海:上海交通大学,2008:35-55.YE Qing.Research on oil/water separation characteristic and treatment in ASP production water[D].Shanghai:Shanghai Jiaotong University,2008:35-55.
[4]吴迪,孟祥春,张瑞泉,等.大庆油田三元复合驱采出水的稳定性及其反相破乳剂的应用[J].精细化工,2001,18(2):86-88.WU Di,MENG Xiangchun,ZHANG Ruiquan,et al.Stability and chemical treatment of Daqing Oilfield ASP flooding produced water[J].Fine Chemicals,2001,18(2):86-88.
[5]张威,黄斌,王莹莹,等.三元复合驱采出水处理技术研究进展[J].化工环保,2017,37(2):152-158.ZHANG Wei,HUANG Bin,WANG Yingying,et al.Research processes on treatment technologies of alkali/surfactant/polymer(ASP)flooding produced water[J].Environmental Protection of Chemical Industry,2017,37(2):152-158.
[6]孙晓君,于凯,魏金枝,等.4株聚丙烯酰胺降解菌的深度驯化及联合降解作用[J].环境工程学报,2016,10(3):1533-1538.SUN Xiaojun,YU Kai,WEI Jinzhi,et al.Gradient domestication and combined degradation of four polyacrylamide degrading bactria strains[J].Chinese Journal of Environmental Engineering,2016,10(3):1533-1538.
[7]包木太,彭杰,陈庆国.微生物对聚丙烯酰胺降解作用的研究进展[J].化工进展,2011,30(9):2080-2086.BAO Mutai,PENG Jie,CHEN Qingguo.Research progress of biodegradation of polyacrylamide by microorganisms[J].Chemical Industry and Engineering Progress,2011,30(9):2080-2086.
[8]MILLER J.Biodegradable surfactants aid the development of environmentally acceptable drilling-fluid additives[R].SPE,2007:106506.
[9]COBIANCO S,ALBONICO P.Thermophilic enzymes for filter-cake removal at high temperature[R].SPE,2007:107756.
[10]李宜强,沈传海,景贵成,等.微生物降解HPAM的机理及其应用[J].石油勘探与开发,2006,33(6):739-742.LI Yiqiang,SHEN Chuanhai,JING Guicheng,et al.Mechanism of HPAM-biodegradation and its application[J].Petroleum Exploration and Development,2006,33(6):739-742.
[11]任国领,徐晶雪,王晶,等.聚丙烯酰胺降解菌筛选与分子生物学鉴定[J].东北石油大学学报,2014,38(4):67-71.REN Guoling,XU Jingxue,WANG Jing,et al.Isolation and molecular identification of polyacrylamide degrading bacteria[J].Journal of Northeast Petroleum University,2014,38(4):67-71.
[12]张虹,任国领,徐晶雪,等.大庆油田低渗透油藏假单胞菌的筛选及性能评价[J].东北林业大学学报,2015,43(7):143-145.ZHANG Hong,REN Guoling,XU Jingxue,et al.Isolation and performance evaluation on pseudomonas aeruginosa strain of low permeability reservoir in Daqing Oilfield[J].Journal of Northeast Forestry University,2015,43(7):143-145.
[13]任国领,袁红梅,侯兆伟,等.大庆油田油藏微生物基因组DNA提取及分析[J].大庆石油学院学报,2010,34(4):89-93.REN Guoling,YUAN Hongmei,HOU Zhaowei,et al.Genomic DNA extraction and analysis of oil reservoir microorganism in Daqing Oilfields[J].Journal of Daqing Petroleum Institute,2010,34(4):89-93.
[14]黄永红,袁红梅,伍晓林,等.大庆油田低渗透油藏微生物群落结构研究[J].大庆石油学院学报,2009,33(4):63-66.HUANG Yonghong,YUAN Hongmei,WU Xiaolin,et al.Analysis of microbial community structure for the Daqing Oilfield in lowpermeability reservoir block areas[J].Journal of Daqing Petroleum Institute,2009,33(4):63-66.
[15]王笠沣.复合驱产出聚合物质量浓度的准确跟踪测定[J].大庆石油地质与开发,2015,34(1):108-115.WANG Lifeng.Accurate tracking and measurement of the mass concentration for the produced polymer in the complex flooding[J].Petroleum Geology and Oilfield Development in Daqing,2015,34(1):108-115.
[16]THOMPSON J D,GIBSON T J,PLEWNIAK F,et al.The CLUSTAL-X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J].Nucleic Acids Res,1997,25(24):4876-4882.
[17]李淑芹,韦微,包龙,等.聚丙烯酰胺降解菌的分离鉴定及其降解特性[J].东北农业大学学报,2013,44(5):24-28.LI Shuqin,WEI Wei,BAO Long,et al.Separation and identification of polyacrylamide degrading-bacteria and its degradation characteristics[J].Journal of Northeast Agricultural University,2013,44(5):24-28.
[18]李殿杰.聚丙烯酰胺降解菌株的分离及其油田含聚污水生物强化处理研究[J].环境科学与管理,2018,43(1):113-117.LI Dianjie.Isolation of HPAM degrading bacteria strain and polymer containing oilfield wastewater treatment[J].Environmental Science and Management,2018,43(1):113-117.
[19]陈庆国.聚丙烯酰胺降解菌的筛选及降解含聚丙烯酰胺污水的室内研究[D].青岛:中国海洋大学,2009:35-55.CHEN Qingguo.Screening and valuing of HPAM-degrading bacteria and studies on biochemical treatment of wastewater containing HAPM[D].Qingdao:Ocean University of China,2009:35-55.
[20]李淑芹,孙靖,韦微,等.三株聚丙烯酰胺降解菌联合降解特性研究[J].东北农业大学学报,2015,46(8):21-25.LI Shuqin,SUN Jing,WEI Wei,et al.Study on the characteristics of combination degradation by three PAM degrading-bacteria[J].Journal of Northeast Agricultural University,2015,46(8):21-25.