EMS诱变技术及其在小麦研究中的应用进展
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摘要
甲基磺酸乙酯(EMS)是一种常用的化学诱变剂,能诱发产生高频率的基因和染色体突变。近年来,依托突变体的创建为基础的基因定位、克隆和功能基因研究得以迅速拓展。从小麦EMS诱变、最佳处理剂量与表型、生理生化、分子标记鉴定,产量、品质、抗逆、抗感病等关键性状突变体筛选以及小麦异源易位系创制与诱导小麦条锈菌毒性突变、突变库的利用等多方面对我国在EMS诱变及其在小麦研究中的应用进展进行了综述,并对EMS技术的发展前景及趋势进行了展望,以期为今后的诱变育种提供研究参考。
Ethyl methane sulfonate(EMS)is a common chemical mutagen, which can induce high frequency gene and chromosome mutations. In recent years, gene mapping, cloning and functional gene research based on mutant creation have been rapidly expanded.The progress of EMS mutagenesis and its application in wheat research in China was reviewed from the aspects of EMS mutagenesis,optimal treatment dose and phenotype, physiological and biochemical, molecular marker identification, screening of mutants of key traits such as yield, quality, stress resistance and susceptibility, as well as the creation and induction of wheat heterologous translocation lines,the utilization of toxicity mutation and mutation bank of stripe rust, and so on. The development prospects and trends were forecasted to provide research references for mutation breeding in the future.
Ethyl methane sulfonate(EMS)is a common chemical mutagen, which can induce high frequency gene and chromosome mutations. In recent years, gene mapping, cloning and functional gene research based on mutant creation have been rapidly expanded.The progress of EMS mutagenesis and its application in wheat research in China was reviewed from the aspects of EMS mutagenesis,optimal treatment dose and phenotype, physiological and biochemical, molecular marker identification, screening of mutants of key traits such as yield, quality, stress resistance and susceptibility, as well as the creation and induction of wheat heterologous translocation lines,the utilization of toxicity mutation and mutation bank of stripe rust, and so on. The development prospects and trends were forecasted to provide research references for mutation breeding in the future.
引文
[1]刘翔. EMS诱变技术在植物育种中的研究进展[J].激光生物学报,2014,23(3):197-201.
[2]薛芳,褚洪雷,胡志伟,等. EMS对新春11小麦抗性淀粉和农艺性状的诱变效果[J].麦类作物学报,2010,30(3):431-434.
[3]张维宏,安哲,范学锋,等. EMS诱导小麦TcLr19感叶锈病突变体的筛选[J].华北农学报,2016,31(4):88-93.
[4]王国芬,井金学,王美南,等.化学诱变剂EMS对小麦条锈菌夏孢子萌发率的影响[J].西北农林科技大学学报(自然科学版),2004,32(7):53-56.
[5]丁延庆,高旭,程斌,等. EMS诱变对小麦品种贵协3号种子的影响[J].农技服务,2018,35(3):46-47.
[6]李晓,郭会君. EMS诱发小麦京411突变群体构建及Wx-A1基因突变体鉴定[J].核农学报,2016,30(11):2081-2087.
[7]程志锋,杨文香,刘大群,等.60Coγ射线与EMS对小麦近等基因系Tc Lr10的复合诱变[J].华北农学报,2008,23(Z):92-95.
[8]闫智慧,郭会君,徐荣旗,等. TILLING技术的发展及其在不同植物中的应用[J].核农学报,2014,28(2):224-233.
[9]孙玉龙,朱欣欣,何瑞士,等. EMS诱导的盛农1号小麦突变体筛选与鉴定[J].麦类作物学报,2018,38(7):782-790.
[10]袁秀芳,王丹峰,殷慧娟,等.小麦EMS突变体的创制与性状鉴定[J].山东农业科学,2018,50(7):61-66.
[11]蒋方山,郭营. EMS诱变的小麦基部小穗不孕突变体的鉴定与小穗形态发育[J].麦类作物学报,2008,28(2):249-253.
[12]朱培培,连少英,刘红杰,等. EMS诱变国麦301突变体库的构建[J].农业科技通讯,2015(3):103-106.
[13]倪永静,朱培培,刘红杰,等. EMS诱导国麦301小麦突变体库的建立与鉴定[J].河南农业科学,2015,44(4):42-45,92.
[14]陆燕,赵天祥,刘国祥,等.小麦矮秆圆粒突变体的鉴定与分析[J].植物遗传资源学报,2014,15(1):160-164.
[15]徐艳花,陈锋. EMS诱变的普通小麦豫农201突变体库的构建与初步分析[J].麦类作物学报,2010,30(4):625-629.
[16]赵天祥,孔秀英. EMS诱变六倍体小麦偃展4110的形态突变体鉴定与分析[J].中国农业科学,2009,42(3):755-764.
[17]闫炯,付晶,刘桂茹,等. EMS诱变小麦河农822的SSR及SDS-PAGE鉴定[J].河北农业大学学报,2008,31(1):1-5,11.
[18]许云峰,蒋方山,郭营,等. EMS诱导小麦品种烟农15突变的鉴定和EST-SSR分析[J].核农学报,2008,22(4):410-414.
[19] CHEN S,GAO R,WANG H. Characterization of a novel reduced height gene(Rht23)regulating panicle morphology and plant architecture in bread wheat[J]. Euphytica,2015,203:583-594.
[20] LU Y,XING L. Characterization of a putative new semi-dominant reduced height gene,Rht_NM9,in wheat(Triticum aestivum L.)[J]. Journal of Genetics and Genomics,2015,42:685-698.
[21]张纪元,张平平.以EMS诱变创制软质小麦宁麦9号高分子量谷蛋白亚基突变体[J].作物学报,2014,40(9):1579-1584.
[22]张贞彩,戴双,程敦公,等. EMS诱变对小麦淀粉理化特性的影响[J].南方农业学报,2011,42(5):479-482.
[23]柳学余,周华平,缪炳良.γ射线、微波与EMS复合处理改良小麦品质的研究:理化诱变因素对诱发提高小麦蛋白质含量的效果[J].核农学报,1985(4):21-25.
[24]王瑾,刘桂茹,杨学举. EMS诱变小麦愈伤组织选择抗旱突变体的研究[J].中国农学通报,2005,21(12):190-193.
[25]马燕欣.南农感白粉病突变体的创制及在抗病机理研究中的应用[D].南京:南京农业大学,2013.
[26]李韬,骆孟.抗赤霉病小麦地方品种黄方柱和海盐种EMS突变体的变异分析[J].植物遗传资源学报,2016,17(6):1092-1098.
[27]陈洋,高兰英. EMS诱导小麦易位系YW642突变体的鉴定与分子标记分析[J].核农学报,2011,25(4):617-621,651.
[28]施巾帼,胡小元,范庆霞,等.利用核辐射诱发小麦抗白粉病突变新种质[J].核农学报,1996,10(4):199-204.
[29]姚秋燕,王国芬. EMS诱导小麦条锈菌毒性突变的研究[J].西北农林科技大学学报(自然科学版),2006,34(6):120-123.
[30] BIE T D. Mass production of intergeneric chromosomal translocatons through pollen irradiation of Triticum durum-Haynaldia villosa amphiploid[J]. J Interg Plant Biol,2007,49(11):1-8.
[31]敬樊,王亮明,武军,等.利用甲基磺酸乙酯(EMS)诱导小麦-华山新麦草染色体易位的研究[J].农业生物技术学报,2015,23(5):561-570.
[32] WANG Z,LI J,CHEN S,et al. Poaceae-specific MS1 encodes a phospholipid-binding protein for male fertility in bread wheat[J].PNAS,2017,114:12614-12619.
[33] WANG W,HAO Q,LI Q,et al. The cytokinin-regulated sucrose metabolism involved in the stay-green phenotype[J]. PLo S ONE,2016,11(8):0161351.
[34]XIA C,ZHANG L. A TRIM insertion in the promoter of Ms2 causes male sterility in wheat[J]. Nature Communications,2017,8:15407.
[35]ZHANG X Y,LIU G X,ZHANG L C,et al. Fine mapping of a novel heading date gene,TaHdm605,in hexaploidwheat[J]. Frontiers in Plant Science,2017,9:1059.
[36] ZHANG G. Characterization and mapping of QTLs on chromosome2D for grain size and yield traits using a mutant line induced by EMS in wheat[J]. The Crop Journal,2015,3(2):135-144.
[37] ZHAI S,LI G,SUN Y,et al. Genetic analysis of phytoene synthase1(Psy1)gene function and regulation in common wheat[J]. BMC Plant Biology,2016,16:22.
[38] KRASILEVA K V,VASQUEZ-GROSS H A. Uncovering hidden variation in polyploid wheat[J]. PNAS,2017,114:913-921.
[39]庄丽芳,亓增军.植物染色体诱变研究与应用进展[J].南京农业大学学报,2018,41(1):3-17.
[2]薛芳,褚洪雷,胡志伟,等. EMS对新春11小麦抗性淀粉和农艺性状的诱变效果[J].麦类作物学报,2010,30(3):431-434.
[3]张维宏,安哲,范学锋,等. EMS诱导小麦TcLr19感叶锈病突变体的筛选[J].华北农学报,2016,31(4):88-93.
[4]王国芬,井金学,王美南,等.化学诱变剂EMS对小麦条锈菌夏孢子萌发率的影响[J].西北农林科技大学学报(自然科学版),2004,32(7):53-56.
[5]丁延庆,高旭,程斌,等. EMS诱变对小麦品种贵协3号种子的影响[J].农技服务,2018,35(3):46-47.
[6]李晓,郭会君. EMS诱发小麦京411突变群体构建及Wx-A1基因突变体鉴定[J].核农学报,2016,30(11):2081-2087.
[7]程志锋,杨文香,刘大群,等.60Coγ射线与EMS对小麦近等基因系Tc Lr10的复合诱变[J].华北农学报,2008,23(Z):92-95.
[8]闫智慧,郭会君,徐荣旗,等. TILLING技术的发展及其在不同植物中的应用[J].核农学报,2014,28(2):224-233.
[9]孙玉龙,朱欣欣,何瑞士,等. EMS诱导的盛农1号小麦突变体筛选与鉴定[J].麦类作物学报,2018,38(7):782-790.
[10]袁秀芳,王丹峰,殷慧娟,等.小麦EMS突变体的创制与性状鉴定[J].山东农业科学,2018,50(7):61-66.
[11]蒋方山,郭营. EMS诱变的小麦基部小穗不孕突变体的鉴定与小穗形态发育[J].麦类作物学报,2008,28(2):249-253.
[12]朱培培,连少英,刘红杰,等. EMS诱变国麦301突变体库的构建[J].农业科技通讯,2015(3):103-106.
[13]倪永静,朱培培,刘红杰,等. EMS诱导国麦301小麦突变体库的建立与鉴定[J].河南农业科学,2015,44(4):42-45,92.
[14]陆燕,赵天祥,刘国祥,等.小麦矮秆圆粒突变体的鉴定与分析[J].植物遗传资源学报,2014,15(1):160-164.
[15]徐艳花,陈锋. EMS诱变的普通小麦豫农201突变体库的构建与初步分析[J].麦类作物学报,2010,30(4):625-629.
[16]赵天祥,孔秀英. EMS诱变六倍体小麦偃展4110的形态突变体鉴定与分析[J].中国农业科学,2009,42(3):755-764.
[17]闫炯,付晶,刘桂茹,等. EMS诱变小麦河农822的SSR及SDS-PAGE鉴定[J].河北农业大学学报,2008,31(1):1-5,11.
[18]许云峰,蒋方山,郭营,等. EMS诱导小麦品种烟农15突变的鉴定和EST-SSR分析[J].核农学报,2008,22(4):410-414.
[19] CHEN S,GAO R,WANG H. Characterization of a novel reduced height gene(Rht23)regulating panicle morphology and plant architecture in bread wheat[J]. Euphytica,2015,203:583-594.
[20] LU Y,XING L. Characterization of a putative new semi-dominant reduced height gene,Rht_NM9,in wheat(Triticum aestivum L.)[J]. Journal of Genetics and Genomics,2015,42:685-698.
[21]张纪元,张平平.以EMS诱变创制软质小麦宁麦9号高分子量谷蛋白亚基突变体[J].作物学报,2014,40(9):1579-1584.
[22]张贞彩,戴双,程敦公,等. EMS诱变对小麦淀粉理化特性的影响[J].南方农业学报,2011,42(5):479-482.
[23]柳学余,周华平,缪炳良.γ射线、微波与EMS复合处理改良小麦品质的研究:理化诱变因素对诱发提高小麦蛋白质含量的效果[J].核农学报,1985(4):21-25.
[24]王瑾,刘桂茹,杨学举. EMS诱变小麦愈伤组织选择抗旱突变体的研究[J].中国农学通报,2005,21(12):190-193.
[25]马燕欣.南农感白粉病突变体的创制及在抗病机理研究中的应用[D].南京:南京农业大学,2013.
[26]李韬,骆孟.抗赤霉病小麦地方品种黄方柱和海盐种EMS突变体的变异分析[J].植物遗传资源学报,2016,17(6):1092-1098.
[27]陈洋,高兰英. EMS诱导小麦易位系YW642突变体的鉴定与分子标记分析[J].核农学报,2011,25(4):617-621,651.
[28]施巾帼,胡小元,范庆霞,等.利用核辐射诱发小麦抗白粉病突变新种质[J].核农学报,1996,10(4):199-204.
[29]姚秋燕,王国芬. EMS诱导小麦条锈菌毒性突变的研究[J].西北农林科技大学学报(自然科学版),2006,34(6):120-123.
[30] BIE T D. Mass production of intergeneric chromosomal translocatons through pollen irradiation of Triticum durum-Haynaldia villosa amphiploid[J]. J Interg Plant Biol,2007,49(11):1-8.
[31]敬樊,王亮明,武军,等.利用甲基磺酸乙酯(EMS)诱导小麦-华山新麦草染色体易位的研究[J].农业生物技术学报,2015,23(5):561-570.
[32] WANG Z,LI J,CHEN S,et al. Poaceae-specific MS1 encodes a phospholipid-binding protein for male fertility in bread wheat[J].PNAS,2017,114:12614-12619.
[33] WANG W,HAO Q,LI Q,et al. The cytokinin-regulated sucrose metabolism involved in the stay-green phenotype[J]. PLo S ONE,2016,11(8):0161351.
[34]XIA C,ZHANG L. A TRIM insertion in the promoter of Ms2 causes male sterility in wheat[J]. Nature Communications,2017,8:15407.
[35]ZHANG X Y,LIU G X,ZHANG L C,et al. Fine mapping of a novel heading date gene,TaHdm605,in hexaploidwheat[J]. Frontiers in Plant Science,2017,9:1059.
[36] ZHANG G. Characterization and mapping of QTLs on chromosome2D for grain size and yield traits using a mutant line induced by EMS in wheat[J]. The Crop Journal,2015,3(2):135-144.
[37] ZHAI S,LI G,SUN Y,et al. Genetic analysis of phytoene synthase1(Psy1)gene function and regulation in common wheat[J]. BMC Plant Biology,2016,16:22.
[38] KRASILEVA K V,VASQUEZ-GROSS H A. Uncovering hidden variation in polyploid wheat[J]. PNAS,2017,114:913-921.
[39]庄丽芳,亓增军.植物染色体诱变研究与应用进展[J].南京农业大学学报,2018,41(1):3-17.