H6亚型禽流感病毒厦门分离株全基因序列分析
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摘要
为了解2017年分离自厦门市某活禽屠宰场的H6N6亚型禽流感病毒(AIV)的遗传变异特征,本研究对分离到的4株H6N6亚型AIV进行全基因组序列测定、遗传进化分析和特殊位点的氨基酸分析。结果显示:4株分离株的HA裂解位点和受体结合位点均符合低致病性AIV特征,但其中1株分离株NA存在颈部11个氨基酸的缺失。NS1发生P42S突变、M1发生N30D、T215A突变、M2发生V27I的突变。8个基因节段均属于欧亚谱系,内部基因与H5N1、H5N6、H6N1及H6N2 AIV亲缘关系密切。本研究结果表明,4株H6N6分离株均表现为低致病性AIV特征,但也存在与耐药性、毒力增强等相关的突变,基因来源呈现多样性。本研究结果为本地区AIV生物学特性研究及其疾病防控提供科学依据。
Four H6N6 subtype avian influenza virus(AIV)were isolated from a live-poultry slaughtering factory of Xiamen during 2017.The complete genomes were sequenced,the evolutionary relationship and the amino acid sequence were analyzed,The result showed that the four isolates were categorized as low pathogenic AIV,with typical HA cleavage site and receptor binding sites.One of the isolates had a special NA sequence which had 11 continuous amino acid deletion in the stalk region.Mutation P42S of the NS1,N30D.of the M1 and V27I of the M2 were found in all isolates.The eight genes of the four isolates belonged to the Eurasian avian lineage and the six inner genes were closely related to H5N1,H5N6,H6N1 and H6N2.The results of this study showed that 4 H6N6 isolates were characterized by low pathogenic AIV,but there were also mutations related to drug resistance and virulence enhancement,and the genetic diversity was varied.The result will lay the foundation of biological characteristic study and provide a basis for prevention and control of AIV infection.
Four H6N6 subtype avian influenza virus(AIV)were isolated from a live-poultry slaughtering factory of Xiamen during 2017.The complete genomes were sequenced,the evolutionary relationship and the amino acid sequence were analyzed,The result showed that the four isolates were categorized as low pathogenic AIV,with typical HA cleavage site and receptor binding sites.One of the isolates had a special NA sequence which had 11 continuous amino acid deletion in the stalk region.Mutation P42S of the NS1,N30D.of the M1 and V27I of the M2 were found in all isolates.The eight genes of the four isolates belonged to the Eurasian avian lineage and the six inner genes were closely related to H5N1,H5N6,H6N1 and H6N2.The results of this study showed that 4 H6N6 isolates were characterized by low pathogenic AIV,but there were also mutations related to drug resistance and virulence enhancement,and the genetic diversity was varied.The result will lay the foundation of biological characteristic study and provide a basis for prevention and control of AIV infection.
引文
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[2]Yuan Jian,Zhang Lei,Kan Xian-zhao,et al.Origin and molecular characteristics of a novel 2013 avian influenza A(H6N1)virus causing human infection in Taiwan[J].Clin Infect Dis,2013,57(9):1367-1368.
[3]Olsen B,Munster V J,Wallensten A,et al.Global patterns of influenza a virus in wild birds[J].Science,2006,312(5772):384-388.
[4]Süss J,Sch覿fer J,Sinnecker H,et al.Influenza virus subtypes in aquatic birds of eastern Germany[J].Arch Virol,1994,135(1-2):101-114.
[5]Honda E,Kida H,Yanagawa R,et al.Survey of influenza viruses in feral birds in 1979 and isolation of a strain possessing Hav6Nav5 from cloaca of an eastern dunlin[J].Jpn J Vet Res,1981,29(3-4):83-87.
[6]Abao L N,Jamsransuren D,Bui V N,et al.Surveillance and characterization of avian influenza viruses from migratory water birds in eastern Hokkaido,the northern part of Japan,2009-2010[J].Virus Genes,2013,46(2):323-329.
[7]Morales A C,Hilt D A,Williams S M,et al.Biologic characterization of H4,H6,and H9 type low pathogenicity avian influenza viruses from wild birds in chickens and turkeys[J].Avian Dis,2009,53(4):552-562.
[8]Duan Lian,Zhu Hua-chen,Wang Jia,et al.Influenza virus surveillance in migratory ducks and sentinel ducks at Poyang Lake,China[J].Influenza Other Respir Viruses,2011,5 Suppl1:65-68.
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[10]Huang Kai,Zhu Hua-chen,Fan Xiao-hui,et al.Establishment and lineage replacement of H6 influenza viruses in domestic ducks in southern China[J].J Virol,2012,86(11):6075-6083.