环介导等温扩增技术(LAMP)在肉源性检测中的应用及前景展望
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摘要
近年来,肉类掺假事件时有发生,给消费者和相关食品安全监督部门造成一定的困扰,同时威胁着消费者的身体健康。传统意义上的检测方法如酶联检测法及PCR法均存在一定的局限性,限制其在市场上进行快速的肉类鉴别。环介导等温扩增(LAMP)是一种新型核酸扩增技术,其灵敏度高、特异性强,不依赖专业仪器设备,操作简单,已开始被广泛应用于食源性微生物的检验。本文从LAMP原理出发,阐述了LAMP技术在肉源性检测中的应用,对LAMP技术的发展前景进行了展望。
In recent years, meat adulteration incidents have occurred from time to time, causing a certain amount of trouble to consumers and related food safety supervision departments, while threatening the health of consumers. Traditional detection methods such as ELISA and PCR have some limitations, which restrict the rapid identification of meat in the market. Application and prospect of loop-mediated isothermal amplification(LAMP) is a novel nucleic acid amplification technique with high sensitivity, specificity, no dependence on professional instruments and simple operation. It has been widely used in the detection of food-borne microorganisms. Based on the principle of LAMP, this paper expounds the application of LAMP technology in meat-borne detection, and prospects the development prospect of LAMP technology.
In recent years, meat adulteration incidents have occurred from time to time, causing a certain amount of trouble to consumers and related food safety supervision departments, while threatening the health of consumers. Traditional detection methods such as ELISA and PCR have some limitations, which restrict the rapid identification of meat in the market. Application and prospect of loop-mediated isothermal amplification(LAMP) is a novel nucleic acid amplification technique with high sensitivity, specificity, no dependence on professional instruments and simple operation. It has been widely used in the detection of food-borne microorganisms. Based on the principle of LAMP, this paper expounds the application of LAMP technology in meat-borne detection, and prospects the development prospect of LAMP technology.
引文
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[2] Nagamine K,Hase T,Notomi T.Accelerated reaction by loop-mediated isothermal amplification using loop primers[J].Molecular and Cellular Probes.,2002,16(3):223-229.
[3] Bhimani M,Bhanderi B,Roy A.Loop-mediated Isothermal Amplification assay (LAMP) based detection of Pasteurella multocida in cases of haemorrhagic septicaemia and fowl cholera[J].Vet. Ital.,2015,51(2):115-21.
[4] Edwards T,Burke P,Helen B.et al.Loop-mediated isothermal amplification (LAMP) for the rapid detection of mycoplasma genitalium[J].Diagnostic Microbiology & Infectious Disease,2015,83(1):13-17.
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[8] 郑洋妹,陈信忠.环介导等温扩增技术检测动物病原研究进展[J].生物技术通报,2009(S1):108-112.
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[10] Whyte P,Mc Gill K,Collins J.D,et al.The prevalence and PCR detection of Salmonella contamination in raw poultry[J].Veterinary Microbiology,2002,89(1):53-60.
[11] Bartlett S E,Davidson W S.FINS (forensically informative nucleotide sequencing): A procedure for identifying the animal origin of biological specimens[J]. BioTechniques.1992,12(3):408-411.
[12] Hopwood A J,Fairbrother K S,Lockley A K,et al.An actin gene-related polymerase chain reaction(PCR) test for identification of chicken in meat mixtures[J].Meat Science,1999,53(4):227-231.
[13] Chamberlain J S,Gibbs R A,Ranier J E,et al.Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification.[J].Nucleic Acids Research,1988,16(23):11141-11143.
[14] 宗卉,范万红,温燕辉.应用多重PCR同时检测牛、羊源性成分[J].中国草食动物,2006(6):21-23.
[15] Karabasanavar N S,Singh S P,Umapathi V,et al.Authentication of carabeef(water buffalo,Bubalus bubalis) using highly specific polymerase chain reaction[J].European Food Research & Technology,2011,233(6):985-989.
[16] YANG Li-tao,LIANG Wan-qi,JIANG Ling-xi,et al.A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids[J].BMC Molecular Biology,2008,9:54.
[17] Shahbazi E,Mollasalehi H,Minai-Tehrani D.Development and evaluation of an improved quantitative loop-mediated isothermal amplification method for rapid detection of Morganella morganii[J].Talanta,2018,19(1):54-58.
[18] 吴阳升,罗淑萍.一种新的高效快速核酸恒温扩增方法——LAMP法[J].生物技术,2004(4):76-78.
[19] Ahmed M U,Hasan Q,Hossain M M,et al.Meat species identification based on the loop mediated isothermal amplification and electrochemical DNA sensor[J].Food Control,2009,21(5):599-605.
[20] 杨丽霞,淑君,彭新凯.环介导等温扩增法检测牛羊肉中的猪肉成分[J].食品与机械,2013,29(5):63-65.
[21] 张英.鉴定羊肉中掺杂猪肉的LAMP检测方法的优化及应用[J].黑龙江畜牧兽医,2018(9):222-224,254.
[22] Roy S,Wei S X,Zhi-Ying J L,et al.A novel,sensitive and label-free loop-mediated isothermal amplification detection method for nucleic acids using luminophore dyes[J].Biosensors and Bioelectronics,2016,86:346-352.
[23] 唐善虎,李雪,王柳.用LAMP法快速鉴别熟制牦牛肉饼中的猪肉和鸡肉成分[J].西南民族大学学报(自然科学版),2018,44(3):237-242.
[24] 徐淑菲,孔繁德,苗丽,等.牛源性成分LAMP检测方法的建立[J].中国动物检疫,2016,33(12):94-99.
[25] 冼钰茵,易敏英,张璜,等.环介导等温扩增技术快速检测肉及肉制品中的牛源性成分[J].食品工业科技,2016,37(7):278-282.
[26] LI Yong-Jin,FAN Jin-Yu.Rapid visual identification of bovine meat by loop mediated isothermal amplification combined with immunochromatographic strip[J].BioChip Journal,2017,11(1):8-13.
[27] 朱珠,兰青阔,赵新,等.饲料中牛、羊源性成分现场可视化检测方法的建立[J].中国畜牧兽医,2014,41(7):95-98.
[28] 李向丽,刘垚,谭贵良,等.基于LAMP法快速检测羊肉及其制品中的猪、鸭和羊源性成分[J].中国食品卫生杂志,2015,27(3):247-252.
[29] 宋涛平,杨丽霞,邱华丽,等.动物源性成分实时等温扩增检测方法的建立[J].食品安全质量检测学报,2017,8(8):3207-3212.
[30] Cho A R,Dong H J,Cho S.Meat species identification using loop-mediated isothermal amplification assay targeting species-specific mitochondrial DNA[J].Korean Journal for Food Science of Animal Resources,2014,34(6):799-807.
[31] Rodriguez M A,Garcia T,Gonzalez I,et al.Identification of goose,mule duck,chicken,turkey,and swine in foie gras by species-specific polymerase chain reaction[J]. J. Agr. Food Chem.,2003,51:1524-1529.
[32] Zahradnik C,Martzy R,Robert L,et al.Loop-mediated isothermal amplification (LAMP) for the detection of horse meat in meat and processed meat products[J].Food Analytical Methods,2015,8(6):1576-1581
[33] 刘少宁,陈智,张志民,等.鉴别绵羊肉中狐狸源性成分的环介导等温扩增检测方法的建立[J].中国食品卫生杂志,2016,28(1):75-78.
[2] Nagamine K,Hase T,Notomi T.Accelerated reaction by loop-mediated isothermal amplification using loop primers[J].Molecular and Cellular Probes.,2002,16(3):223-229.
[3] Bhimani M,Bhanderi B,Roy A.Loop-mediated Isothermal Amplification assay (LAMP) based detection of Pasteurella multocida in cases of haemorrhagic septicaemia and fowl cholera[J].Vet. Ital.,2015,51(2):115-21.
[4] Edwards T,Burke P,Helen B.et al.Loop-mediated isothermal amplification (LAMP) for the rapid detection of mycoplasma genitalium[J].Diagnostic Microbiology & Infectious Disease,2015,83(1):13-17.
[5] Yamazaki Y,Oba E,Kashiwagi N,et al.Development of a loop-mediated isothermal amplification assay for rapid and simple detection of Erysipelothrix rhusiopathiae[J].Lett. Appl. Microbiol.,2014,58(4):362-369.
[6] Khamlor T,Pongpiachan P,Parnpai R,et al.Bovine embryo sex determination by multiplex loop-mediated isothermal amplification[J].Theriogenology,2015,83(5):891-896.
[7] Hirayama H,Kageyama S,Moriyasu S,et al.Embryo sexing and sex chromosomal chimerism analysis by loop-mediated isothermal amplification in cattle and water buffaloes[J].J. Reprod. Dev.,2013,59(4):321-326.
[8] 郑洋妹,陈信忠.环介导等温扩增技术检测动物病原研究进展[J].生物技术通报,2009(S1):108-112.
[9] Notomi T,Mori Y,Tomita N,et al.Loop-mediated isothermal amplification(LAMP): Principle,features,and future prospects[J].Journal of Microbiology,2015,53(1):1-5.
[10] Whyte P,Mc Gill K,Collins J.D,et al.The prevalence and PCR detection of Salmonella contamination in raw poultry[J].Veterinary Microbiology,2002,89(1):53-60.
[11] Bartlett S E,Davidson W S.FINS (forensically informative nucleotide sequencing): A procedure for identifying the animal origin of biological specimens[J]. BioTechniques.1992,12(3):408-411.
[12] Hopwood A J,Fairbrother K S,Lockley A K,et al.An actin gene-related polymerase chain reaction(PCR) test for identification of chicken in meat mixtures[J].Meat Science,1999,53(4):227-231.
[13] Chamberlain J S,Gibbs R A,Ranier J E,et al.Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification.[J].Nucleic Acids Research,1988,16(23):11141-11143.
[14] 宗卉,范万红,温燕辉.应用多重PCR同时检测牛、羊源性成分[J].中国草食动物,2006(6):21-23.
[15] Karabasanavar N S,Singh S P,Umapathi V,et al.Authentication of carabeef(water buffalo,Bubalus bubalis) using highly specific polymerase chain reaction[J].European Food Research & Technology,2011,233(6):985-989.
[16] YANG Li-tao,LIANG Wan-qi,JIANG Ling-xi,et al.A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids[J].BMC Molecular Biology,2008,9:54.
[17] Shahbazi E,Mollasalehi H,Minai-Tehrani D.Development and evaluation of an improved quantitative loop-mediated isothermal amplification method for rapid detection of Morganella morganii[J].Talanta,2018,19(1):54-58.
[18] 吴阳升,罗淑萍.一种新的高效快速核酸恒温扩增方法——LAMP法[J].生物技术,2004(4):76-78.
[19] Ahmed M U,Hasan Q,Hossain M M,et al.Meat species identification based on the loop mediated isothermal amplification and electrochemical DNA sensor[J].Food Control,2009,21(5):599-605.
[20] 杨丽霞,淑君,彭新凯.环介导等温扩增法检测牛羊肉中的猪肉成分[J].食品与机械,2013,29(5):63-65.
[21] 张英.鉴定羊肉中掺杂猪肉的LAMP检测方法的优化及应用[J].黑龙江畜牧兽医,2018(9):222-224,254.
[22] Roy S,Wei S X,Zhi-Ying J L,et al.A novel,sensitive and label-free loop-mediated isothermal amplification detection method for nucleic acids using luminophore dyes[J].Biosensors and Bioelectronics,2016,86:346-352.
[23] 唐善虎,李雪,王柳.用LAMP法快速鉴别熟制牦牛肉饼中的猪肉和鸡肉成分[J].西南民族大学学报(自然科学版),2018,44(3):237-242.
[24] 徐淑菲,孔繁德,苗丽,等.牛源性成分LAMP检测方法的建立[J].中国动物检疫,2016,33(12):94-99.
[25] 冼钰茵,易敏英,张璜,等.环介导等温扩增技术快速检测肉及肉制品中的牛源性成分[J].食品工业科技,2016,37(7):278-282.
[26] LI Yong-Jin,FAN Jin-Yu.Rapid visual identification of bovine meat by loop mediated isothermal amplification combined with immunochromatographic strip[J].BioChip Journal,2017,11(1):8-13.
[27] 朱珠,兰青阔,赵新,等.饲料中牛、羊源性成分现场可视化检测方法的建立[J].中国畜牧兽医,2014,41(7):95-98.
[28] 李向丽,刘垚,谭贵良,等.基于LAMP法快速检测羊肉及其制品中的猪、鸭和羊源性成分[J].中国食品卫生杂志,2015,27(3):247-252.
[29] 宋涛平,杨丽霞,邱华丽,等.动物源性成分实时等温扩增检测方法的建立[J].食品安全质量检测学报,2017,8(8):3207-3212.
[30] Cho A R,Dong H J,Cho S.Meat species identification using loop-mediated isothermal amplification assay targeting species-specific mitochondrial DNA[J].Korean Journal for Food Science of Animal Resources,2014,34(6):799-807.
[31] Rodriguez M A,Garcia T,Gonzalez I,et al.Identification of goose,mule duck,chicken,turkey,and swine in foie gras by species-specific polymerase chain reaction[J]. J. Agr. Food Chem.,2003,51:1524-1529.
[32] Zahradnik C,Martzy R,Robert L,et al.Loop-mediated isothermal amplification (LAMP) for the detection of horse meat in meat and processed meat products[J].Food Analytical Methods,2015,8(6):1576-1581
[33] 刘少宁,陈智,张志民,等.鉴别绵羊肉中狐狸源性成分的环介导等温扩增检测方法的建立[J].中国食品卫生杂志,2016,28(1):75-78.