喀斯特地区白刺花营养物质和酚类物质的含量动态
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摘要
以贵州喀斯特地区饲用灌木白刺花(Sophora davidii)为研究对象,探讨其不同生育期常规营养物质和酚类物质的含量变化趋势。结果表明,粗蛋白在营养期、开花期、结荚期显著高于成熟期(P <0.05),无氮浸出物、粗灰分和粗脂肪含量随白刺花生育期延长而降低(P <0.05);干物质、粗纤维、酸性洗涤纤维、中性洗涤纤维和钙含量随生育期延长而升高(P <0.05);单宁含量在白刺花各生育时期内差异不显著(P> 0.05),但有降低的趋势,其他酚类物质随着生育期延长而降低(P <0.05)。开花期和结荚期单宁含量较营养期低,且这两个生育期粗蛋白含量和饲料相对值高,具有较好的饲喂价值。综合考虑,建议白刺花的最佳利用时期为开花期到结荚期。本研究可为白刺花在贵州喀斯特山地畜牧业中的高效利用提供重要的理论支撑。
The dynamic variations of nutrient and phenolic content in Sophora davidii in a Karst region of the Guizhou Province were investigated in the study. Results showed that the content of crude protein in the vegetative, flowering, and seed set stages was higher than in the maturation stage(P < 0.05). The content of nitrogen free extract, crude ash, and ether extracts decreased with the increasing growth period(P < 0.05). The content of dry matter, crude fiber, acid detergent fiber,neutral detergent fiber, and calcium increased with the increasing growth period(P < 0.05). The content of tannin had no significant relationship with the different growth stages(P > 0.05); however, other phenolics decreased with the increasing growth period(P < 0.05). The content of tannin in the flowering and seed set stages was more suitable for feeding animals than in the vegetative stage, while higher crude protein and relative forage values were also obtained in the former.Considering all factors, we suggest that the best utilization period for feeding animals with Sophora davidii is from the flowering to seed set stages. This study can provide theoretical support for the efficient use of Sophora davidii in animal husbandry in the Karst areas of Guizhou Province.
The dynamic variations of nutrient and phenolic content in Sophora davidii in a Karst region of the Guizhou Province were investigated in the study. Results showed that the content of crude protein in the vegetative, flowering, and seed set stages was higher than in the maturation stage(P < 0.05). The content of nitrogen free extract, crude ash, and ether extracts decreased with the increasing growth period(P < 0.05). The content of dry matter, crude fiber, acid detergent fiber,neutral detergent fiber, and calcium increased with the increasing growth period(P < 0.05). The content of tannin had no significant relationship with the different growth stages(P > 0.05); however, other phenolics decreased with the increasing growth period(P < 0.05). The content of tannin in the flowering and seed set stages was more suitable for feeding animals than in the vegetative stage, while higher crude protein and relative forage values were also obtained in the former.Considering all factors, we suggest that the best utilization period for feeding animals with Sophora davidii is from the flowering to seed set stages. This study can provide theoretical support for the efficient use of Sophora davidii in animal husbandry in the Karst areas of Guizhou Province.
引文
[1]李莉,何胜江,王普昶,杨学东,赵丽丽,刘洪来,龙忠富.喀斯特山区特征灌木白刺花的青贮效果.草业科学,2014,31(10):1957-1965.LI L,HE S J,WANG P C,YANG X D,ZHAO L L,LIU H L,LONG Z F.The silage quality of characteristic shrub Sophora davidii in Karst mountainous area.Pratacltural Science,2014,31(10):1957-1965.
[2]刘华荣,龙忠富,杨义成,罗天琼,赵相勇,卢敏.贵州九种灌木饲用价值的初步研究.贵州畜牧兽医,2013,37(4):60-64.LIU H R,LONG Z F,YANG Y C,LUO T Q,ZHANG XY,LU M.Initial research on the nutrition value of nine kinds of shrubs in Guizhou Province.Guizhou Journal of Animal Husbandry&Veterinary Medicine,2013,37(4):60-64.
[3]VILLALBA J J,SPACKMAN C,GOFF B M,KLOTZ J L,GRIGGS T,MACADAM J W.Interaction between a tannin-containing Legume and endophyte-infected tall fescue seed on lambs'feeding behavior and physiology.Journal of Animal Science,2016,94(2):845-857.
[4]艾庆辉,苗又青,麦康森.单宁的抗营养作用与去除方法的研究进展.中国海洋大学学报(自然科学版),2011,41(Z1):33-40.AI Q H,MIAN Y Q,MAI K S.The anti-nutritional effcets and the degradations of dietary tannins:A review.Periodica of Ocean University of China,2011,41(Z1):33-40.
[5]王妍君,谢开云,赵祥,董宽虎.植物单宁及其对动物的作用研究进展.草原与草坪,2011,31(4):82-86.WANG Y J,XIE K Y,ZHAO X,DONG K H.Research progress on vegetable tannins and its effect on animals.Grassland and Turf,2011,31(4):82-86.
[6]张子仪.中国饲料学.北京:中国农业出版社,2000.ZHANG Z Y.Chinese Feed Science.Beijing:China Agriculture Press,2000.
[7]李凤涛.60Co-γ辐射对白刺花诱变效应研究.贵阳:贵州大学硕士学位论文,2015.LI F T.The studies on irradiation effects of 60Co-γray on Sophora viciifolia.Master Thesis.Guiyang:Guizhou University,2015.
[8]马林.贵州白刺花遗传变异研究.贵阳:贵州大学硕士学位论文,2015.MA L.Study on genetic variation of Sophora davidii in Guizhou Province.Master Thesis.Guiyang:Guizhou University,2015.
[9]陈青,杨小生,朱海燕,郝小江,宋宝安.贵州产白刺花种子的化学成分研究.中草药,2006(7):986-987.CHEN Q,YANG X S,ZHU H Y,HAO X J,SONG B A.Study on chemical constituents of Sophora davidii seeds in Guizhou Province.Chinese Traditional and Herbal Drugs,2006(7):986-987.
[10]马林,王普昶,姚红艳,赵丽丽,陈超,卢立娜.贵州6个白刺花种群种子贮藏蛋白的遗传多样性.贵州农业科学,2015,43(3):5-8.MA L,WANG P C,YAO H Y,ZHAI L L,CHEN C,LU L N.Genetic diversity of seed storage protein of six Sophora davidii populations in Guizhou.Guizhou Agricultural Sciences,2015,43(3):5-8.
[11]雷文英,赵丽丽,简忠领,陈超,宋高翔.白刺花染色体压片技术优化及核型分析.种子,2017,36(8):5-9.LEI W Y,ZHAO L L,JIAN Z L,CHEN C,SONG G X.Optimization of chromosome preparation and karyotype analysis of Sophora davidii.Seed,2017,36(8):5-9.
[12]颜蒙蒙,周洲,王济,谷晓平,肖建勇.喀斯特地区土壤水分随降雨的动态变化研究--以贵阳市花溪区为例.中国岩溶,2016,35(4):446-452.YAN M M,ZHOU Z,WANG J,GU X P,XIAO J Y.Study on the dynamic change of soil moisture in Karst area:A case of Huaxi district in Guiyang City.Carsologica Sinica,2016,35(4):446-452.
[13]张丽英.饲料分析及饲料质量检测技术.第3版.北京:中国农业大学出版社,2007.ZHANG L Y.Feed Analysis and Feed Quality Inspection Technology.3rd Edition.Beijing:China Agricultural University Press,2007.
[14]GOERGING H D,VAN SOEST J P.Forage Fibre Analysis.US Department of Agriculture,Agriculture.Research Service,Washington,DC,1975.
[15]陈秋生.白刺花营养价值和酚类物质动态分析及生产利用.贵阳:贵州大学硕士学位论文,2017.CHEN Q S.The dynamic analysis of nutrition and phenols contents and utilization of Sophora davidii.Master Thesis.Guiyang:Guizhou University,2017.
[16]廉美娜.高寒草甸植物多酚含量动态及抗氧化性研究.兰州:兰州大学硕士学位论文,2013.LIAN M N.Dynamics of total polyphenols and flavonoids concentration of alpine meadow plants and their antioxidant activity.Master Thesis.Lanzhou:Lanzhou University,2013.
[17]ROHWEDER D A,BARNES R F,JORGENSEN N.Proposed hay grading standards based on laboratory analyses for evaluating quality.Journal of Animalence,1983,47(3):747-759.
[18]张吉鹍,卢德勋,刘建新,包赛娜,邹庆华,刘庆华.粗饲料品质评定指数的研究现状及其进展.草业科学,2004,21(9):55-61.ZHANG J K,LU D X,LIU J X,BAO S N,ZOU Q H,LIU Q H.The present research situation and progress of crude fodder quality evaluation index.Pratacultu Ralscience,2004,21(9):55-61.
[19]郭彦军,龙瑞军,张德罡,陈建纲.东祁连山高寒草甸灌木和牧草营养成分含量季节变化动态.草业科学,2001,18(6):36-39.GUO Y J,LONG R J,ZHANG D G,CHEN J G.The seasonal dynamics of nutrient contents in some dominant shrubs and forage herbs on alpine meadow in eastern Qilian mountain,China.Pratacultu Ral Science,2001,18(6):36-39.
[20]BURNS J C,POND K R,FISHER D S,LUGINBUHL J M.Changes in forage quality,ingestive mastication,and digesta kinetics resulting from switchgrass maturity.Journal of Animal Science,1997,75(5):1368.
[21]JOHNSON J A,CATON J S,POLAND W,KIRBY D R,DHUYVETTER D V.Influence of season on dietary composition,intake,and digestion by beef steers grazing mixed-grass prairie in the northern Great Plains.Journal of Animal Science,1998,76(6):1682.
[22]陈碧成,林洁荣,罗宗志,叶健军,焦文静.巨菌草不同生长时间的常规营养成分及氨基酸含量测定.贵州农业科学,2016,44(1):101-113.CHEN B C,LIN J R,LUO Z Z,YE J J,JIAO W J.Determination of routine nutritional ingredients and amino acid contents of Jujuncao during different growth stages.Guizhou Agricultural Sciences,2016,44(1):101-113.
[23]屈言江,师尚礼.陇牧草地早熟禾新品系的营养价值评价.草原与草坪,2017,37(1):51-60.QU Y J,SHI S L.Nutritional evaluation of new lines of Poapratensis cv.Longmu.Grasslangd and Turf,2017,37(1):51-60.
[24]吴永恒.仿刺参饲料中粗灰分、粗纤维及无氮浸出物适宜含量的研究及原料的筛选.青岛:青岛农业大学硕士学位论文,2012.WU Y H.Study on the appropriate content of crude ash,crude fiber and nitrogen free extract in the feed of Apostichopus japonicus and tne screening of the raw material.Master Thesis.Qingdao:Qingdao Agricultural University,2012.
[25]董晓兵,郝明德,肖庆红,吴焜玥,刘公社.不同钾肥施用量对羊草产量、品质及养分吸收的影响.草原与草坪,2015(1):20-26.DONG X B,HAO M D,XIANG Q H,WU K Y,LIU G S.Effect of potassium fertilization on the yield,quality and nutrients up take of Leymus chinensis.Grassland and Turf,2015(1):20-26.
[26]任灵.青藏高原高寒草甸土壤与牧草养分在不同培育措施下的季节动态研究,兰州.甘肃农业大学硕士学位论文,2016.REN L.Seasonal dynamics of nutrients in soil and forage of alpine meadow under different cultivations on Qinghai-Tibet Plateau.Master Thesis.Lanzhou:Gansu Agricultural University,2016.
[27]刘恩科.长期定位不同施肥制度土壤功能衰退与修复研究.泰安:山东农业大学硕士学位论文,2004.LIU E K.Studies on soil function of degradation and restoration in long-term location different fertilization system.Master Thesis.Tai’an:Shandong Agriculture University,2004.
[28]孙羲,郭鹏程.植物营养与肥料.北京:中国农业出版社,1991.SUN X,GUO P C.Plant Nutrition and Fertilizer.Beijing:China Agriculture Press,1991.
[29]文亦芾,曹国军,樊江文,毛华明,罗富成.6种豆科饲用灌木中酚类物质动态变化与体外消化率的关系.草业学报,2009,18(1):32-38.WEN Y F,CAO G J,FAN J W,MAO H M,LUO F C.Relationship between phenols and in vitro digestibility of six legume feeding shrubs.Acta Prataculturae Sinica,2009,18(1):32-38.
[30]COOPER S M,OWEN-SMITH N.Condensed tannins deter feeding by browsing ruminants in a South African Savanna.Oecologia,1985,67(1):142-146.
[31]PROVENZA F D.Postingestive feedback as an elementary determinant of food preference and intake in ruminants.Journal of Range Management,1995,48(1):2-17.
[32]郭彦军.高寒草甸几种牧草和灌木缩合单宁含量动态及其饲用价值.兰州:甘肃农业大学硕士学位论文,2000.GUO Y J.The seasonal dynamies of condensed tannins of some dominant grasses and shrubs on alpine meadow and their feeding values.Master Thesis.Louzhou:Gansu Agricultural University,2000.
[33]王俊宏,郑向丽,徐国忠.缩合单宁功能性研究探讨.热带农业科学,2011,31(1):91-95.WANG J H,ZHENG X L,XU G Z.Discussion on the functions of condensed tannins.Chinese Journal of Tropical Agriculture,2011,31(1):91-95.
[34]FEENY P.Seasonal changes in Oak leaf tannins and nutrients as a cause of spring feeding by Wintr Moth Caterpillars.Ecology,1970,51(4):565-581.
[35]字学娟.主要热带乔灌木饲用价值评定及其单宁含量动态分析.海口:海南大学硕士学位论文,2010.ZI X J.Feeding values assessment of the major tropical trees and shrubs and the dynamic of tannins.Master Thesis.Haikou:Hainan University,2010.
[36]姚娜,易显凤,丘金花,庞天德,赖志强,韦锦益.四种南方豆科灌木饲料在华南地区的比较试验.草业科学,2017,34(4):772-776.YAO N,YI X F,QIU J H,PANG T D,LAI Z Q,WEI J Y.Comparison of four fodder species of Leguminous shrubs in southern China.Pratacultural Science,2017,34(4):772-776.
[37]宋超,靳晓丽,田新会,杜文华.不同红三叶品种生产性能及异黄酮含量的比较.草原与草坪,2012,32(5):47-52.SONG C,JIN X L,TIAN X H,DU W H.Adaptability and isoflavone content on different red clover varieties in Lanzhou.Grassland and Turf,2012,32(5):47-52.
[38]李岩,徐智明,朱德建,王霞霞,李杨.10个美国紫花苜蓿品种生长和营养特性比较.草原与草坪,2015,35(1):89-92.LI Y,XU Z M,ZHU D J,WANG X X,LI Y.Comparison of growth and nutrition characteristics of ten alfalfa varieties introduced from America.Grassland and Turf,2015,35(1):89-92.
[39]JACKSON F S,BARRY T N,LASCANO C,PALMER B.The extractable and bound condensed tannin content of leaves from tropical tree,shrub and forage legumes.Journal of the Science of Food&Agriculture,2015,71(1):103-110.
[40]赵一广,刁其玉,邓凯东,刘洁,姜成钢,屠焰.反刍动物甲烷排放的测定及调控技术研究进展.动物营养学报,2011,23(5):726-734.ZHAO Y G,DIAO Q Y,DENG K D,LIU J,JIANG C G,TU Y.Measure ments and modulation of methane emission from ruminants.Chinese Journal of Animal Nutrition,2011,23(5):726-734.
[41]张本瑜,师尚礼.73份俄罗斯百脉根的营养价值和相对饲喂价值的评价.草原与草坪,2017,37(1):67-78.ZHANG B Y,SHI S L.Evaluation of nutritive value and RFV of 73 Lotus corniculatus germplasms of Russia.Grassland and Turf,2017,37(1):67-78.
[42]李茂,字学娟,周汉林,刘国道,侯冠彧,徐铁山.海南省部分热带灌木饲用价值评定.动物营养学报,2012,24(1):85-94.LI M,ZI X J,ZHOU H L,LIU G D,HOU G Y,XU T S.Feeding value assessment of some tropical shrubs in Hainan Province.Chinese Journal of Animal Nutrition,2012,24(1):85-94.
[43]李茂,陈艳琴,字学娟,周汉林.山蚂蝗属植物饲用价值评价.中国草地学报,2013,35(6):53-57.LI M,CHEN Y Q,ZI X J,ZHOU H L.Feeding values assessment of species of Desmodium.Chinese Journal of Grassland,2013,35(6):53-57.
[2]刘华荣,龙忠富,杨义成,罗天琼,赵相勇,卢敏.贵州九种灌木饲用价值的初步研究.贵州畜牧兽医,2013,37(4):60-64.LIU H R,LONG Z F,YANG Y C,LUO T Q,ZHANG XY,LU M.Initial research on the nutrition value of nine kinds of shrubs in Guizhou Province.Guizhou Journal of Animal Husbandry&Veterinary Medicine,2013,37(4):60-64.
[3]VILLALBA J J,SPACKMAN C,GOFF B M,KLOTZ J L,GRIGGS T,MACADAM J W.Interaction between a tannin-containing Legume and endophyte-infected tall fescue seed on lambs'feeding behavior and physiology.Journal of Animal Science,2016,94(2):845-857.
[4]艾庆辉,苗又青,麦康森.单宁的抗营养作用与去除方法的研究进展.中国海洋大学学报(自然科学版),2011,41(Z1):33-40.AI Q H,MIAN Y Q,MAI K S.The anti-nutritional effcets and the degradations of dietary tannins:A review.Periodica of Ocean University of China,2011,41(Z1):33-40.
[5]王妍君,谢开云,赵祥,董宽虎.植物单宁及其对动物的作用研究进展.草原与草坪,2011,31(4):82-86.WANG Y J,XIE K Y,ZHAO X,DONG K H.Research progress on vegetable tannins and its effect on animals.Grassland and Turf,2011,31(4):82-86.
[6]张子仪.中国饲料学.北京:中国农业出版社,2000.ZHANG Z Y.Chinese Feed Science.Beijing:China Agriculture Press,2000.
[7]李凤涛.60Co-γ辐射对白刺花诱变效应研究.贵阳:贵州大学硕士学位论文,2015.LI F T.The studies on irradiation effects of 60Co-γray on Sophora viciifolia.Master Thesis.Guiyang:Guizhou University,2015.
[8]马林.贵州白刺花遗传变异研究.贵阳:贵州大学硕士学位论文,2015.MA L.Study on genetic variation of Sophora davidii in Guizhou Province.Master Thesis.Guiyang:Guizhou University,2015.
[9]陈青,杨小生,朱海燕,郝小江,宋宝安.贵州产白刺花种子的化学成分研究.中草药,2006(7):986-987.CHEN Q,YANG X S,ZHU H Y,HAO X J,SONG B A.Study on chemical constituents of Sophora davidii seeds in Guizhou Province.Chinese Traditional and Herbal Drugs,2006(7):986-987.
[10]马林,王普昶,姚红艳,赵丽丽,陈超,卢立娜.贵州6个白刺花种群种子贮藏蛋白的遗传多样性.贵州农业科学,2015,43(3):5-8.MA L,WANG P C,YAO H Y,ZHAI L L,CHEN C,LU L N.Genetic diversity of seed storage protein of six Sophora davidii populations in Guizhou.Guizhou Agricultural Sciences,2015,43(3):5-8.
[11]雷文英,赵丽丽,简忠领,陈超,宋高翔.白刺花染色体压片技术优化及核型分析.种子,2017,36(8):5-9.LEI W Y,ZHAO L L,JIAN Z L,CHEN C,SONG G X.Optimization of chromosome preparation and karyotype analysis of Sophora davidii.Seed,2017,36(8):5-9.
[12]颜蒙蒙,周洲,王济,谷晓平,肖建勇.喀斯特地区土壤水分随降雨的动态变化研究--以贵阳市花溪区为例.中国岩溶,2016,35(4):446-452.YAN M M,ZHOU Z,WANG J,GU X P,XIAO J Y.Study on the dynamic change of soil moisture in Karst area:A case of Huaxi district in Guiyang City.Carsologica Sinica,2016,35(4):446-452.
[13]张丽英.饲料分析及饲料质量检测技术.第3版.北京:中国农业大学出版社,2007.ZHANG L Y.Feed Analysis and Feed Quality Inspection Technology.3rd Edition.Beijing:China Agricultural University Press,2007.
[14]GOERGING H D,VAN SOEST J P.Forage Fibre Analysis.US Department of Agriculture,Agriculture.Research Service,Washington,DC,1975.
[15]陈秋生.白刺花营养价值和酚类物质动态分析及生产利用.贵阳:贵州大学硕士学位论文,2017.CHEN Q S.The dynamic analysis of nutrition and phenols contents and utilization of Sophora davidii.Master Thesis.Guiyang:Guizhou University,2017.
[16]廉美娜.高寒草甸植物多酚含量动态及抗氧化性研究.兰州:兰州大学硕士学位论文,2013.LIAN M N.Dynamics of total polyphenols and flavonoids concentration of alpine meadow plants and their antioxidant activity.Master Thesis.Lanzhou:Lanzhou University,2013.
[17]ROHWEDER D A,BARNES R F,JORGENSEN N.Proposed hay grading standards based on laboratory analyses for evaluating quality.Journal of Animalence,1983,47(3):747-759.
[18]张吉鹍,卢德勋,刘建新,包赛娜,邹庆华,刘庆华.粗饲料品质评定指数的研究现状及其进展.草业科学,2004,21(9):55-61.ZHANG J K,LU D X,LIU J X,BAO S N,ZOU Q H,LIU Q H.The present research situation and progress of crude fodder quality evaluation index.Pratacultu Ralscience,2004,21(9):55-61.
[19]郭彦军,龙瑞军,张德罡,陈建纲.东祁连山高寒草甸灌木和牧草营养成分含量季节变化动态.草业科学,2001,18(6):36-39.GUO Y J,LONG R J,ZHANG D G,CHEN J G.The seasonal dynamics of nutrient contents in some dominant shrubs and forage herbs on alpine meadow in eastern Qilian mountain,China.Pratacultu Ral Science,2001,18(6):36-39.
[20]BURNS J C,POND K R,FISHER D S,LUGINBUHL J M.Changes in forage quality,ingestive mastication,and digesta kinetics resulting from switchgrass maturity.Journal of Animal Science,1997,75(5):1368.
[21]JOHNSON J A,CATON J S,POLAND W,KIRBY D R,DHUYVETTER D V.Influence of season on dietary composition,intake,and digestion by beef steers grazing mixed-grass prairie in the northern Great Plains.Journal of Animal Science,1998,76(6):1682.
[22]陈碧成,林洁荣,罗宗志,叶健军,焦文静.巨菌草不同生长时间的常规营养成分及氨基酸含量测定.贵州农业科学,2016,44(1):101-113.CHEN B C,LIN J R,LUO Z Z,YE J J,JIAO W J.Determination of routine nutritional ingredients and amino acid contents of Jujuncao during different growth stages.Guizhou Agricultural Sciences,2016,44(1):101-113.
[23]屈言江,师尚礼.陇牧草地早熟禾新品系的营养价值评价.草原与草坪,2017,37(1):51-60.QU Y J,SHI S L.Nutritional evaluation of new lines of Poapratensis cv.Longmu.Grasslangd and Turf,2017,37(1):51-60.
[24]吴永恒.仿刺参饲料中粗灰分、粗纤维及无氮浸出物适宜含量的研究及原料的筛选.青岛:青岛农业大学硕士学位论文,2012.WU Y H.Study on the appropriate content of crude ash,crude fiber and nitrogen free extract in the feed of Apostichopus japonicus and tne screening of the raw material.Master Thesis.Qingdao:Qingdao Agricultural University,2012.
[25]董晓兵,郝明德,肖庆红,吴焜玥,刘公社.不同钾肥施用量对羊草产量、品质及养分吸收的影响.草原与草坪,2015(1):20-26.DONG X B,HAO M D,XIANG Q H,WU K Y,LIU G S.Effect of potassium fertilization on the yield,quality and nutrients up take of Leymus chinensis.Grassland and Turf,2015(1):20-26.
[26]任灵.青藏高原高寒草甸土壤与牧草养分在不同培育措施下的季节动态研究,兰州.甘肃农业大学硕士学位论文,2016.REN L.Seasonal dynamics of nutrients in soil and forage of alpine meadow under different cultivations on Qinghai-Tibet Plateau.Master Thesis.Lanzhou:Gansu Agricultural University,2016.
[27]刘恩科.长期定位不同施肥制度土壤功能衰退与修复研究.泰安:山东农业大学硕士学位论文,2004.LIU E K.Studies on soil function of degradation and restoration in long-term location different fertilization system.Master Thesis.Tai’an:Shandong Agriculture University,2004.
[28]孙羲,郭鹏程.植物营养与肥料.北京:中国农业出版社,1991.SUN X,GUO P C.Plant Nutrition and Fertilizer.Beijing:China Agriculture Press,1991.
[29]文亦芾,曹国军,樊江文,毛华明,罗富成.6种豆科饲用灌木中酚类物质动态变化与体外消化率的关系.草业学报,2009,18(1):32-38.WEN Y F,CAO G J,FAN J W,MAO H M,LUO F C.Relationship between phenols and in vitro digestibility of six legume feeding shrubs.Acta Prataculturae Sinica,2009,18(1):32-38.
[30]COOPER S M,OWEN-SMITH N.Condensed tannins deter feeding by browsing ruminants in a South African Savanna.Oecologia,1985,67(1):142-146.
[31]PROVENZA F D.Postingestive feedback as an elementary determinant of food preference and intake in ruminants.Journal of Range Management,1995,48(1):2-17.
[32]郭彦军.高寒草甸几种牧草和灌木缩合单宁含量动态及其饲用价值.兰州:甘肃农业大学硕士学位论文,2000.GUO Y J.The seasonal dynamies of condensed tannins of some dominant grasses and shrubs on alpine meadow and their feeding values.Master Thesis.Louzhou:Gansu Agricultural University,2000.
[33]王俊宏,郑向丽,徐国忠.缩合单宁功能性研究探讨.热带农业科学,2011,31(1):91-95.WANG J H,ZHENG X L,XU G Z.Discussion on the functions of condensed tannins.Chinese Journal of Tropical Agriculture,2011,31(1):91-95.
[34]FEENY P.Seasonal changes in Oak leaf tannins and nutrients as a cause of spring feeding by Wintr Moth Caterpillars.Ecology,1970,51(4):565-581.
[35]字学娟.主要热带乔灌木饲用价值评定及其单宁含量动态分析.海口:海南大学硕士学位论文,2010.ZI X J.Feeding values assessment of the major tropical trees and shrubs and the dynamic of tannins.Master Thesis.Haikou:Hainan University,2010.
[36]姚娜,易显凤,丘金花,庞天德,赖志强,韦锦益.四种南方豆科灌木饲料在华南地区的比较试验.草业科学,2017,34(4):772-776.YAO N,YI X F,QIU J H,PANG T D,LAI Z Q,WEI J Y.Comparison of four fodder species of Leguminous shrubs in southern China.Pratacultural Science,2017,34(4):772-776.
[37]宋超,靳晓丽,田新会,杜文华.不同红三叶品种生产性能及异黄酮含量的比较.草原与草坪,2012,32(5):47-52.SONG C,JIN X L,TIAN X H,DU W H.Adaptability and isoflavone content on different red clover varieties in Lanzhou.Grassland and Turf,2012,32(5):47-52.
[38]李岩,徐智明,朱德建,王霞霞,李杨.10个美国紫花苜蓿品种生长和营养特性比较.草原与草坪,2015,35(1):89-92.LI Y,XU Z M,ZHU D J,WANG X X,LI Y.Comparison of growth and nutrition characteristics of ten alfalfa varieties introduced from America.Grassland and Turf,2015,35(1):89-92.
[39]JACKSON F S,BARRY T N,LASCANO C,PALMER B.The extractable and bound condensed tannin content of leaves from tropical tree,shrub and forage legumes.Journal of the Science of Food&Agriculture,2015,71(1):103-110.
[40]赵一广,刁其玉,邓凯东,刘洁,姜成钢,屠焰.反刍动物甲烷排放的测定及调控技术研究进展.动物营养学报,2011,23(5):726-734.ZHAO Y G,DIAO Q Y,DENG K D,LIU J,JIANG C G,TU Y.Measure ments and modulation of methane emission from ruminants.Chinese Journal of Animal Nutrition,2011,23(5):726-734.
[41]张本瑜,师尚礼.73份俄罗斯百脉根的营养价值和相对饲喂价值的评价.草原与草坪,2017,37(1):67-78.ZHANG B Y,SHI S L.Evaluation of nutritive value and RFV of 73 Lotus corniculatus germplasms of Russia.Grassland and Turf,2017,37(1):67-78.
[42]李茂,字学娟,周汉林,刘国道,侯冠彧,徐铁山.海南省部分热带灌木饲用价值评定.动物营养学报,2012,24(1):85-94.LI M,ZI X J,ZHOU H L,LIU G D,HOU G Y,XU T S.Feeding value assessment of some tropical shrubs in Hainan Province.Chinese Journal of Animal Nutrition,2012,24(1):85-94.
[43]李茂,陈艳琴,字学娟,周汉林.山蚂蝗属植物饲用价值评价.中国草地学报,2013,35(6):53-57.LI M,CHEN Y Q,ZI X J,ZHOU H L.Feeding values assessment of species of Desmodium.Chinese Journal of Grassland,2013,35(6):53-57.