车前草提取物与牛血清白蛋白和金属离子相互作用的研究
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摘要
车前草分布遍及全国,为车前科车前属的多年生草本植物。车前草作为我国传统中药常被用来进行清热利尿通淋、祛痰和解毒。但关于车前草提取物与牛血清白蛋白和金属离子的研究鲜有报道。该研究对车前草水提取物和乙醇提取物分别进行了荧光性质和吸收光谱的研究,研究了其与牛血清白蛋白和金属离子的相互作用。结果表明:车前草水提取物和乙醇提取物都使牛血清白蛋白自身荧光减弱,并与金属离子产生了不同的相互作用,其吸收光谱也产生了不同程度的变化。该研究结果为进一步研究车前草在疾病治疗中发挥药效机制提供一定的参考价值。
Plantago asiatica distributed throughout China.Plantago asiatica,as a traditional Chinese medicine,is often used for clearing heat and diuresis,dispelling phlegm and detoxification.However,few studies have been reported on the extract of Plantago asiatica with bovine serum albumin and metal ions.In this study,the fluorescence propertiesand absorption spectrumof the water and ethanol extract of Plantago asiatica were studied,and the interactions with bovine serum albumin and metal ions were also studied.The results showed that both the water extract of the Plantago asiatica and the ethanol extract of the Plantago asiatica weakened the fluorescence of the bovine serum albumin and produced different interactions with the metal ions.The absorption spectra also changed in different degrees.The results provide a reference for further study on the efficacy mechanism of Plantago asiatica in disease treatment.
Plantago asiatica distributed throughout China.Plantago asiatica,as a traditional Chinese medicine,is often used for clearing heat and diuresis,dispelling phlegm and detoxification.However,few studies have been reported on the extract of Plantago asiatica with bovine serum albumin and metal ions.In this study,the fluorescence propertiesand absorption spectrumof the water and ethanol extract of Plantago asiatica were studied,and the interactions with bovine serum albumin and metal ions were also studied.The results showed that both the water extract of the Plantago asiatica and the ethanol extract of the Plantago asiatica weakened the fluorescence of the bovine serum albumin and produced different interactions with the metal ions.The absorption spectra also changed in different degrees.The results provide a reference for further study on the efficacy mechanism of Plantago asiatica in disease treatment.
引文
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[10]付彩霞,魏光成,杨杨.维生素E与牛血清白蛋白的相互作用及金属离子对其影响的研究[J].化学研究与应用,2017,29(12):1800-1805.
[11]Ebba Bárány,Bergdahl I A,Bratteby L E,et al.Relationships between trace element concentrations in human blood and serum[J].Toxicol Lett,2002,134(1-3):177-184.
[2]陈红云,申元英.车前草几种提取物的抗菌活性研究[J].大理学院学报,2012,11(3):21-23.
[3]李丽,刘质净,时东方,等.车前草中苯乙醇苷类化合物的抗氧化活性研究[J].江苏农业科学,2010,(1):275-277.
[4]李艳,白明,韩鹏然,等.鲜车前草糊剂外用对大鼠创伤性溃疡的影响[J].中华中医药杂志,2016,(4):1238-1240.
[5]黄筱钧,张朝贵.车前草对呼吸道合胞病毒体外抑制作用的研究[J].湖北民族学院学报:医学版,2015,(2):1-3.
[6]于继英,王春雨,斯日古楞,等.车前草和瓜尔豆提取物中的生物活性物质[J].饲料广角,2016,(1):45-47.
[7]张增杰.车前草单方治疗痛风[J].中国民间疗法,2008,(9):59-59.
[8]梁飞,肖云丽,徐艳霞,等.车前草提取物对三种储粮害虫的触杀活性研究[J].湖北农业科学,2013,52(22):5493-5495.
[9]张秋兰,逯露,李璋,等.光谱法结合原子力显微镜研究呋喃唑酮与牛血清白蛋白的作用机理[J].分析科学学报,2017,(6):752-756.
[10]付彩霞,魏光成,杨杨.维生素E与牛血清白蛋白的相互作用及金属离子对其影响的研究[J].化学研究与应用,2017,29(12):1800-1805.
[11]Ebba Bárány,Bergdahl I A,Bratteby L E,et al.Relationships between trace element concentrations in human blood and serum[J].Toxicol Lett,2002,134(1-3):177-184.