升清降浊胶囊对慢性肾衰竭大鼠CHOP、Caspase-12表达的影响
摘要
目的:探讨升清降浊胶囊对慢性肾功能衰竭(CRF)大鼠CHOP、Caspase-12表达的影响。方法:30mg/mL腺嘌呤灌胃21d建立SD大鼠肾衰模型。72只大鼠随机分为6组:空白组,模型组,肾衰宁组,升清降浊低、中、高剂量组,每组12只。治疗28d后,观察各组大鼠体重变化,全自动生化分析仪检测大鼠肾功能,应用TUNEL法检测肾组织细胞凋亡情况,Western blot检测CHOP和Caspase-12肾内表达水平。结果:模型组BUN、Scr较空白组明显升高(P<0.01);TUNEL结果显示空白组肾组织细胞有少量凋亡,而模型组细胞凋亡数明显增多(P<0.01);模型组CHOP、Caspase-12表达量较空白组显著增强(P<0.01)。升清降浊胶囊低剂量能抑制慢性肾功能衰竭大鼠肾组织细胞凋亡,与模型组相比差异有统计学意义(P<0.05),CHOP和Caspase-12表达亦有统计学意义(P<0.01)。结论:升清降浊胶囊低剂量能保护腺嘌呤导致的大鼠肾损害,可能与其抑制ERS相关凋亡分子CHOP、Caspase-12高表达有关。
引文
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[5]Ron D, Habener JF. CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant-negative inhibitor of gene transcription[J].Genes&Development, 1992, 6(3):439-453.
[6]McCullough K D, Martindale J L, Klotz L O, et al. Gadd1 53 sensitizes cells to endoplasmic reticulum stress by down-regulating Bcl2 and perturbing the cellular redox state[J]. Molecular and cellular biology,2001, 21(4):1 249-1 259.
[7]Song S, Lee H, Kam TI, et al.E2-25K/Hip-2 regulates caspase-12 in ER stress-mediated A neurotoxicity[J].Journal of Cell Biology, 2008,182(4):675-684.
[2]陈娜子,姜潮,李校堃.内质网应激与疾病[J].中国生物工程杂志,2016, 36(1):76-85.
[3]郑平东,朱燕俐,丁名城,等.用腺嘌呤制作慢性肾功能衰竭动物模型[J].中华肾脏病杂志, 1989, 5(6):342.
[4]王卫民.肾衰I号汤防治腺嘌呤所致大鼠慢性肾功能衰竭的实验研究[J].中国中医药科技, 2001, 8(2):79-80.
[5]Ron D, Habener JF. CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant-negative inhibitor of gene transcription[J].Genes&Development, 1992, 6(3):439-453.
[6]McCullough K D, Martindale J L, Klotz L O, et al. Gadd1 53 sensitizes cells to endoplasmic reticulum stress by down-regulating Bcl2 and perturbing the cellular redox state[J]. Molecular and cellular biology,2001, 21(4):1 249-1 259.
[7]Song S, Lee H, Kam TI, et al.E2-25K/Hip-2 regulates caspase-12 in ER stress-mediated A neurotoxicity[J].Journal of Cell Biology, 2008,182(4):675-684.