肾移植围术期红细胞免疫功能变化探讨
|
摘要
目的探讨肾移植受者围术期血红细胞免疫功能及CD4、CD8淋巴细胞的变化。方法选取2013年1月—2017年3月肾移植受者25例,25例健康体检者作为对照组,分别于术后3 d、7 d、14 d、1个月、3个月采集外周血行红细胞免疫功能和CD4、CD8淋巴细胞数检测,观察红细胞免疫功能和淋巴细胞数的变化情况。结果肾移植受者术前红细胞花环率低于对照组,其表面膜分子CD35、CD58、CD59表达也低于对照组,而CD4、CD8淋巴细胞数稍低于对照组,术后红细胞花环率、CD4、CD8淋巴细胞数呈现先下降后逐步恢复的过程,其表面膜分子CD35于术后呈现先降后升现象,CD58、CD59于术后呈现先轻微上升,后逐步恢复。结论肾移植受者术前红细胞免疫功能低于对照组,术后有所改善,其变化与淋巴细胞变化基本一致,红细胞免疫可能参与到淋巴细胞及肾移植受者机体免疫功能的调节。
Objective To observe the changes of erythrocyte immune function and CD4,CD8 lymphocyte number during perioperative period in renal transplant recipients.Methods A total of 25 recipients with kidney transplantation during January 2013 and March 2017 were chosen as the observation group,and 25 healthy controls taking physical examinations were chosen as the control group.The erythrocyte immune function and the number of CD4,CD8 lymphocytes before and on the 3 d,7 d,14 d,1 month and 3 months after the transplantation was lymphocytes.Results The red cell rosette in the observation group were lower than those in the control group,the CD35,CD58,CD59 expressions before the transplantation in the observation group were lower than those in the control group.The number of CD4,CD8 lymphocytes were slightly lower than the control group.Red cell rosette and the number of CD4 and CD8 lymphocytes decreased at first,and then gradually recovered.The surface CD35 dropped at first after the operation,and the CD58 and CD59 showed a slight increase after the operation,and then gradually recovered.Conclusion The immune function of erythrocyte was weak in recipients before transplantation,but the function can be recovered after transplantation.Its changes were basically consistent with lymphocyte changes,the erythrocytes transplant recipients.
Objective To observe the changes of erythrocyte immune function and CD4,CD8 lymphocyte number during perioperative period in renal transplant recipients.Methods A total of 25 recipients with kidney transplantation during January 2013 and March 2017 were chosen as the observation group,and 25 healthy controls taking physical examinations were chosen as the control group.The erythrocyte immune function and the number of CD4,CD8 lymphocytes before and on the 3 d,7 d,14 d,1 month and 3 months after the transplantation was lymphocytes.Results The red cell rosette in the observation group were lower than those in the control group,the CD35,CD58,CD59 expressions before the transplantation in the observation group were lower than those in the control group.The number of CD4,CD8 lymphocytes were slightly lower than the control group.Red cell rosette and the number of CD4 and CD8 lymphocytes decreased at first,and then gradually recovered.The surface CD35 dropped at first after the operation,and the CD58 and CD59 showed a slight increase after the operation,and then gradually recovered.Conclusion The immune function of erythrocyte was weak in recipients before transplantation,but the function can be recovered after transplantation.Its changes were basically consistent with lymphocyte changes,the erythrocytes transplant recipients.
引文
[1]郭峰.红细胞免疫及其调节功能测定方法[J].免疫学杂志,19 90,6(1):60-65.
[2]胡金川,江朝光,田亚平,等.深、浅低温体外循环手术对人红细胞和淋巴细胞免疫影响的比较[J/CD].中华临床医师杂志(电子版),2013,7(6):2469-2473.
[3]王旭,陈虹,范铁艳,等.肝肾移植患者红细胞免疫功能的研究进展与设想[J].医学与哲学,2012,33(10):49-51.
[4]孔祥瑞,石炳毅.红细胞在移植免疫中作用的研究进展[J].中华器官移植杂志,2014,35(10):634-636.
[5]王旭,陈虹,沈中阳,等.肝移植患者围手术期红细胞免疫功能与T、B淋巴细胞亚群的关系[J].中国组织工程与临床康复,2011,15(44):8197-8200.
[6]孔祥瑞,肖漓,刘志佳,等.肾移植受者红细胞CD35、CD58、CD59表达变化[J].解放军医药杂志,2015,27(7):10-13.
[7]王吉,罗萍,张冬梅.重组人促红细胞生成素对慢性肾功能衰竭患者红细胞免疫的影响[J].吉林大学学报(医学版),2006,32(6):1071-1073.
[8]沈颖,刘华,李丰良,等.慢性肾功能衰竭患者红细胞膜免疫分子表达的临床研究[J].河北医药,2010,32(1):51-52。
[9]何玉林,邓玲.CD58分子研究概况[J].中国医药指南,2012,10(2):50-52.
[10]Crepin T,Carron C,Roubiou C,et al.ATG-induced accelerated immune senescence:clinical implications in renal transplant recipients[J].Am J Transplant,2015,15(4):1028-1038.
[11]Scemla A,Gerber S,Duquesne A,et al.Dramatic improvement of severe cryptococcosis-induced immune reconstitution syndrome with adalimumab in a renal transplant recipient[J].Am JTransplant,2015,15(2):560-564.--
[2]胡金川,江朝光,田亚平,等.深、浅低温体外循环手术对人红细胞和淋巴细胞免疫影响的比较[J/CD].中华临床医师杂志(电子版),2013,7(6):2469-2473.
[3]王旭,陈虹,范铁艳,等.肝肾移植患者红细胞免疫功能的研究进展与设想[J].医学与哲学,2012,33(10):49-51.
[4]孔祥瑞,石炳毅.红细胞在移植免疫中作用的研究进展[J].中华器官移植杂志,2014,35(10):634-636.
[5]王旭,陈虹,沈中阳,等.肝移植患者围手术期红细胞免疫功能与T、B淋巴细胞亚群的关系[J].中国组织工程与临床康复,2011,15(44):8197-8200.
[6]孔祥瑞,肖漓,刘志佳,等.肾移植受者红细胞CD35、CD58、CD59表达变化[J].解放军医药杂志,2015,27(7):10-13.
[7]王吉,罗萍,张冬梅.重组人促红细胞生成素对慢性肾功能衰竭患者红细胞免疫的影响[J].吉林大学学报(医学版),2006,32(6):1071-1073.
[8]沈颖,刘华,李丰良,等.慢性肾功能衰竭患者红细胞膜免疫分子表达的临床研究[J].河北医药,2010,32(1):51-52。
[9]何玉林,邓玲.CD58分子研究概况[J].中国医药指南,2012,10(2):50-52.
[10]Crepin T,Carron C,Roubiou C,et al.ATG-induced accelerated immune senescence:clinical implications in renal transplant recipients[J].Am J Transplant,2015,15(4):1028-1038.
[11]Scemla A,Gerber S,Duquesne A,et al.Dramatic improvement of severe cryptococcosis-induced immune reconstitution syndrome with adalimumab in a renal transplant recipient[J].Am JTransplant,2015,15(2):560-564.--