超声微泡破坏技术不同辐照强度对兔心肌的生物学效应研究
|
摘要
目的研究超声微泡破坏技术不同辐照强度对兔心肌细胞及心肌组织的生物学效应,优化出影响兔心肌的最佳辐照条件。方法用不同强度0.5、0.75、1.0、1.5W/cm~2辐照心肌细胞与微泡的混合物,辐照时间30s,间隔10s,再辐照30s,显微镜下观察心肌细胞形态结构变化。20只日本大耳白兔随机分为4组,分别用上述强度及时间辐照兔心肌组织,行HE染色。结果不同功率辐照后IVS、LVPW运动幅度及EF与FS差异无统计学意义。0.5 W/cm~2和0.75 W/cm~2辐照后心肌细胞形态未见明显改变,1.0W/cm~2及1.5W/cm~2辐照后少量心肌细胞出现轻度及以上损伤表现。0.5W/cm~2辐照后心肌组织无水肿、出血;0.75W/cm~2出现轻微水肿,无出血;1.0W/cm~2及1.5W/cm~2辐照后心肌组织出现少量出血并炎细胞浸润。结论超声辐照强度在1.0W/cm~2以下时不会造成心肌细胞及组织损伤,1.0W/cm~2以上时能使兔心肌产生轻度损伤及一定程度的炎症反应。
Objective To study the biological effects of different radiation intensitiesof ultrasound microbubble destruction on rabbit cardiac myocyte and myocardial tissue.Optimize the optimal irradiation conditions of rabbit myocardium.Methods The mixture of myocardial cells and microbubbles was irradiated with different radiation intensities of 0.5,0.75,1.0 W/cm~2 and 1.5 W/cm~2.Irradiation of 30 s,interval 10 s,and then irradiated for 30 s.The morphological and structural changes of cardiac myocytes were observed under microscope.20 Japanese white rabbits were randomly divided into 4 groups.Irradiatedthe rabbit myocardial tissue with the intensity and time mentioned above and stained with HE.Results There was no significant difference in the motion amplitude of IVS and LVPW and EF and FS after different power irradiation.After 0.5 W/cm~2 and 0.75 W/cm~2 irradiation,the morphology of cardiac myocyteswere not changed significantly.After 1.0 W/cm~2 and 1.5 W/cm~2 irradiation,a small number of cardiac myocyteswere mild or more injured.No edema and bleeding of myocardial tissuewere seen after 0.5 W/cm~2 irradiation.Slightly edema without hemorrhage of myocardial tissue were seen after irradiation of 0.75 W/cm~2.After 1.0 W/cm~2 and 1.5 W/cm~2 irradiation,there was a small amount of bleeding and inflammatory cell infiltration.Conclusions Ultrasound intensity below 1.0 W/cm~2 could not cause cardiac myocytes and tissues injured.When the ultrasound intensity was above 1.0 W/cm~2,it could cause mild myocardial injury and a certain degree of inflammatory reaction.
Objective To study the biological effects of different radiation intensitiesof ultrasound microbubble destruction on rabbit cardiac myocyte and myocardial tissue.Optimize the optimal irradiation conditions of rabbit myocardium.Methods The mixture of myocardial cells and microbubbles was irradiated with different radiation intensities of 0.5,0.75,1.0 W/cm~2 and 1.5 W/cm~2.Irradiation of 30 s,interval 10 s,and then irradiated for 30 s.The morphological and structural changes of cardiac myocytes were observed under microscope.20 Japanese white rabbits were randomly divided into 4 groups.Irradiatedthe rabbit myocardial tissue with the intensity and time mentioned above and stained with HE.Results There was no significant difference in the motion amplitude of IVS and LVPW and EF and FS after different power irradiation.After 0.5 W/cm~2 and 0.75 W/cm~2 irradiation,the morphology of cardiac myocyteswere not changed significantly.After 1.0 W/cm~2 and 1.5 W/cm~2 irradiation,a small number of cardiac myocyteswere mild or more injured.No edema and bleeding of myocardial tissuewere seen after 0.5 W/cm~2 irradiation.Slightly edema without hemorrhage of myocardial tissue were seen after irradiation of 0.75 W/cm~2.After 1.0 W/cm~2 and 1.5 W/cm~2 irradiation,there was a small amount of bleeding and inflammatory cell infiltration.Conclusions Ultrasound intensity below 1.0 W/cm~2 could not cause cardiac myocytes and tissues injured.When the ultrasound intensity was above 1.0 W/cm~2,it could cause mild myocardial injury and a certain degree of inflammatory reaction.
引文
[1] JUFFERMANS LJ,ANNEMIEKE D,JONGENELEN C A,et al.Ultrasound and microbubble-induced intra-and intercellular bioeffects in primary endothelial cells[J].Ultrasound in Medicine and Biology,2009,35(11):1917-1927.
[2]钟世根,王志刚,凌智瑜,等.不同强度超声破坏微泡对犬心肌组织的生物学效应[J].中国医学影像技术,2009,25(10):1738-1740.
[3]张群霞,王志刚,冉海涛,等.不同超声强度及微泡对基因和组织作用的实验研究[J].中华超声影像学杂志,2005,14(4):304-306.
[4] BEKEREDJIAN R,CHEN S Y,FRENKEL P A,et al.Ultrasound-targeted microbubble destruction can repeatedly direct highly specific plasmid expression to the heart[J].Circulation,2013,108(8):1022-1026.
[5]李秋颖,刘政,李佩惊,等.微泡介导下脉冲式聚焦超声空化导致血管损伤的实验研究[J].中国超声医学杂志,2008,24(4):297-299.
[6] SONG S,SHEN Z,CHEN L,et al.Explorations of high-intensity therapeutic ultrasound and microbubble-mediated gene delivery in mouse liver[J].Gene Ther,2011,18(10):1006-1014.
[7]许婷婷,吴风林,罗利红,等.超声激励微泡增强VX2移植瘤细胞膜通透性的实验研究[J].中国超声医学杂志,2013,29(6):568-571.
[2]钟世根,王志刚,凌智瑜,等.不同强度超声破坏微泡对犬心肌组织的生物学效应[J].中国医学影像技术,2009,25(10):1738-1740.
[3]张群霞,王志刚,冉海涛,等.不同超声强度及微泡对基因和组织作用的实验研究[J].中华超声影像学杂志,2005,14(4):304-306.
[4] BEKEREDJIAN R,CHEN S Y,FRENKEL P A,et al.Ultrasound-targeted microbubble destruction can repeatedly direct highly specific plasmid expression to the heart[J].Circulation,2013,108(8):1022-1026.
[5]李秋颖,刘政,李佩惊,等.微泡介导下脉冲式聚焦超声空化导致血管损伤的实验研究[J].中国超声医学杂志,2008,24(4):297-299.
[6] SONG S,SHEN Z,CHEN L,et al.Explorations of high-intensity therapeutic ultrasound and microbubble-mediated gene delivery in mouse liver[J].Gene Ther,2011,18(10):1006-1014.
[7]许婷婷,吴风林,罗利红,等.超声激励微泡增强VX2移植瘤细胞膜通透性的实验研究[J].中国超声医学杂志,2013,29(6):568-571.