巨菌草促生菌的筛选及其促生效应
|
摘要
以陕西和新疆两地采集的巨菌草为材料,从中筛选分离得到内生菌株103株,采用解磷、固氮和产IAA等筛选标准,对初筛菌株进行促生能力的测定,其中4株菌株同时具有多种促生作用;通过形态观察、生理生化和16Sr DNA序列分析,对这4株促生菌株进行分类鉴定,结果显示菌株XJ–4和SX–12均归属于肠杆菌属(Enterobacter),SX–30归属于芽孢杆菌属(Bacillus),XJ–12归属于寡养单胞菌属(Stenotrophomonas)。采用单菌接种和多菌混合接种小麦盆栽试验,测定促生菌的促生效应,结果表明,与对照组相比,多菌混合接种对小麦的促生效应优于单独接种处理的,经SX–30、SX–12和XJ–4混合菌液处理,小麦叶绿素含量增加了129.94%;经SX–12和XJ–4混合菌液处理,小麦幼苗鲜重增加了182.12%。
In the present study, Pennisetum sinese from Shaanxi and Xinjiang was used as the main research materia, from which 103 strains of endophytic bacteria were isolated. The plant growth promoting ability of these strains were screened based on their capacities of phosphate-solubilizing, nitrogen fixation, and production of indoleacetic acid, and 4 of these strains showed multiple growth promoting effects. Morphological observation, physiological and biochemical and 16 S r DNA sequence analysis were conducted on the 4 growth promoting strains. The results showed strains XJ-4 and strain SX-12 belonged to Enterobacter, strain SX-30 belonged to Bacillus, and strain XJ-12 belonged to Stenotrophomonas. The growth promoting effects were further tested by wheat inoculation studies with individual strain or with strain combinations. Compared with the control group, growth promoting effects of the strain combinations were better than that of single strain: the chlorophyll content of wheat increased by 129.94% after treated with combination of strain SX-30, strain SX-12, and strain XJ-4; the fresh weight of wheat seedlings increased by 182.12% with combination of strain SX-12 and strain XJ-4.
In the present study, Pennisetum sinese from Shaanxi and Xinjiang was used as the main research materia, from which 103 strains of endophytic bacteria were isolated. The plant growth promoting ability of these strains were screened based on their capacities of phosphate-solubilizing, nitrogen fixation, and production of indoleacetic acid, and 4 of these strains showed multiple growth promoting effects. Morphological observation, physiological and biochemical and 16 S r DNA sequence analysis were conducted on the 4 growth promoting strains. The results showed strains XJ-4 and strain SX-12 belonged to Enterobacter, strain SX-30 belonged to Bacillus, and strain XJ-12 belonged to Stenotrophomonas. The growth promoting effects were further tested by wheat inoculation studies with individual strain or with strain combinations. Compared with the control group, growth promoting effects of the strain combinations were better than that of single strain: the chlorophyll content of wheat increased by 129.94% after treated with combination of strain SX-30, strain SX-12, and strain XJ-4; the fresh weight of wheat seedlings increased by 182.12% with combination of strain SX-12 and strain XJ-4.
引文
[1]LU Q L,TANG L R,WANG S,et al.An investigation on the characteristics of cellulose nanocrystals from Pennisetum sinese[J].Biomass and Bioenergy,2014,70:267-272.
[2]O’DONNELL A G,SEASMAN M,MACRAE A.Plants and fertilizers as drivers of change in microbial community structure and function in soils[J].Plant and Soil,2001,232(1/2):135-145.
[3]张进国,雷荷仙,黎纪凤,等.巨菌草在不同海拔高度的生长表现[J].贵州农业科学,2013,41(3):112-115.
[4]林兴生,林占熺,林冬梅,等.荒坡地种植巨菌草对土壤微生物群落功能多样性及土壤肥力的影响[J].生态学报,2014,34(15):4304-4312.
[5]林兴生,林占熺,林冬梅,等.低温胁迫5种菌草的抗寒性评价[J].草业学报,2013,22(2):227-234.
[6]林兴生,林占熺,林辉,等.五种菌草苗期对碱胁迫的生理响应及抗碱性评价[J].植物生理学报,2013,49(2):167-174.
[7]谢长海,王培丹,刘艳玲,等.巨菌草对干旱胁迫的生理响应及相关性分析[J].北方园艺,2015(2):129-133.
[8]邓振山,党军龙,张海州,等.植物根际促生菌的筛选及其对玉米的促生效应[J].微生物学通报,2012,39(7):980-988.
[9]邓振山,李超,何园.南瓜中内生菌筛选及其次级代谢产物的分析[J].食品科学,2016,37(23):216-221.
[10]姚晓玲,康前进,熊顺子,等.喜树种子内生放线菌的分离鉴定及抗菌活性物质初分离[J].微生物学通报,2014,41(6):1109-1120.
[11]东秀珠.常见细菌系统鉴定手册[K].北京:科学出版社,2001:58.
[12]张德全,刘涵,廖勇,等.菌落PCR改良技术快速鉴定阿萨希毛孢子[J].中国真菌学杂志,2015,10(1):6-10.
[13]张磊,叶大柠,朱焱,等.10株蜃楼弗朗西斯菌的鉴定与特征分析[J].临床检验杂志,2017,35(4):271-276.
[14]KUMAR A,SINGH R,YADAV A,et al.Isolation and characterization of bacterial endophytes of Curcuma longa L.[J].Biotech,2016,6(1):60.
[15]刘佳莉,方芳,史煦涵,等.2株盐碱地燕麦根际促生菌的筛选及其促生作用研究[J].草业学报,2013,22(2):132-139.
[16]田宏,李凤霞,张德罡,等.草坪草溶磷菌筛选及溶磷能力的初步研究[J].草业科学,2005,22(10):92-96.
[17]田江,彭霞薇,李霞,等.重金属抗性解磷细菌的磷溶解特性研究[J].环境科学,2014,35(6):2334-2340.
[18]ZAHOOR M,IRSHAD M,RAHMAN H,et al.Alleviation of heavy metal toxicity and phytostimulation of Brassica campestris L.by endophytic Mucor sp.MHR-7[J].Ecotoxicology&Environmental Safety,2017,142:139-149.
[19]LIAQAT F,ELTEM R.Identification and characterization of endophytic bacteria isolated from in vitro cultures of peach and pear rootstocks[J].Biotech,2016,6(2):120.
[20]蒋晓玲.解淀粉芽孢杆菌Y19微生物菌肥的研制及其生物效益研究[D].昆明:云南农业大学,2015.
[21]康贻军,程洁,梅丽娟,等.植物根际促生菌的筛选及鉴定[J].微生物学报,2010,50(7):853-861.
[22]姜瑛,吴越,王国文,等.一株固氮解磷菌的筛选鉴定及其对花生的促生作用研究[J].土壤,2015,47(4):689-703.
[23]赵龙飞,徐亚军,常佳丽,等.具ACC脱氨酶活性大豆根瘤内生菌的筛选、抗性及促生作用[J].微生物学报,2016,56(6):1009-1021.
[24]韩文星,姚拓,席琳乔,等.PGPR菌肥制作及其对燕麦生长和品质影响的研究[J].草业学报,2008,17(2):75-84.
[2]O’DONNELL A G,SEASMAN M,MACRAE A.Plants and fertilizers as drivers of change in microbial community structure and function in soils[J].Plant and Soil,2001,232(1/2):135-145.
[3]张进国,雷荷仙,黎纪凤,等.巨菌草在不同海拔高度的生长表现[J].贵州农业科学,2013,41(3):112-115.
[4]林兴生,林占熺,林冬梅,等.荒坡地种植巨菌草对土壤微生物群落功能多样性及土壤肥力的影响[J].生态学报,2014,34(15):4304-4312.
[5]林兴生,林占熺,林冬梅,等.低温胁迫5种菌草的抗寒性评价[J].草业学报,2013,22(2):227-234.
[6]林兴生,林占熺,林辉,等.五种菌草苗期对碱胁迫的生理响应及抗碱性评价[J].植物生理学报,2013,49(2):167-174.
[7]谢长海,王培丹,刘艳玲,等.巨菌草对干旱胁迫的生理响应及相关性分析[J].北方园艺,2015(2):129-133.
[8]邓振山,党军龙,张海州,等.植物根际促生菌的筛选及其对玉米的促生效应[J].微生物学通报,2012,39(7):980-988.
[9]邓振山,李超,何园.南瓜中内生菌筛选及其次级代谢产物的分析[J].食品科学,2016,37(23):216-221.
[10]姚晓玲,康前进,熊顺子,等.喜树种子内生放线菌的分离鉴定及抗菌活性物质初分离[J].微生物学通报,2014,41(6):1109-1120.
[11]东秀珠.常见细菌系统鉴定手册[K].北京:科学出版社,2001:58.
[12]张德全,刘涵,廖勇,等.菌落PCR改良技术快速鉴定阿萨希毛孢子[J].中国真菌学杂志,2015,10(1):6-10.
[13]张磊,叶大柠,朱焱,等.10株蜃楼弗朗西斯菌的鉴定与特征分析[J].临床检验杂志,2017,35(4):271-276.
[14]KUMAR A,SINGH R,YADAV A,et al.Isolation and characterization of bacterial endophytes of Curcuma longa L.[J].Biotech,2016,6(1):60.
[15]刘佳莉,方芳,史煦涵,等.2株盐碱地燕麦根际促生菌的筛选及其促生作用研究[J].草业学报,2013,22(2):132-139.
[16]田宏,李凤霞,张德罡,等.草坪草溶磷菌筛选及溶磷能力的初步研究[J].草业科学,2005,22(10):92-96.
[17]田江,彭霞薇,李霞,等.重金属抗性解磷细菌的磷溶解特性研究[J].环境科学,2014,35(6):2334-2340.
[18]ZAHOOR M,IRSHAD M,RAHMAN H,et al.Alleviation of heavy metal toxicity and phytostimulation of Brassica campestris L.by endophytic Mucor sp.MHR-7[J].Ecotoxicology&Environmental Safety,2017,142:139-149.
[19]LIAQAT F,ELTEM R.Identification and characterization of endophytic bacteria isolated from in vitro cultures of peach and pear rootstocks[J].Biotech,2016,6(2):120.
[20]蒋晓玲.解淀粉芽孢杆菌Y19微生物菌肥的研制及其生物效益研究[D].昆明:云南农业大学,2015.
[21]康贻军,程洁,梅丽娟,等.植物根际促生菌的筛选及鉴定[J].微生物学报,2010,50(7):853-861.
[22]姜瑛,吴越,王国文,等.一株固氮解磷菌的筛选鉴定及其对花生的促生作用研究[J].土壤,2015,47(4):689-703.
[23]赵龙飞,徐亚军,常佳丽,等.具ACC脱氨酶活性大豆根瘤内生菌的筛选、抗性及促生作用[J].微生物学报,2016,56(6):1009-1021.
[24]韩文星,姚拓,席琳乔,等.PGPR菌肥制作及其对燕麦生长和品质影响的研究[J].草业学报,2008,17(2):75-84.