摘要
为构建蚱总科昆虫的系统发育关系,探讨蚱总科传统分类和分子系统发育关系的异同,扩增并测定了16种蚱类昆虫COI基因部分序列,结合NCBI记录,对蚱总科6科17属41种蚱的COI基因597bp序列,分别采用邻接法(NJ)、最简约法(MP)、最大似然法(ML)和贝叶斯法(BI)重建蚱总科的系统发育关系。结果表明:蚱总科为一单系类群,其中蚱科较为进化,其次为刺翼蚱科,其余各科在蚱总科中的位置不确定,枝背蚱科、刺翼蚱科、短翼蚱科、蚱科均不是单系类群;在属的水平上,股沟蚱属、优角蚱属、瘤蚱属是单系类群,瘤蚱属与优角蚱属形成姐妹群,其他属不是单系类群,拟后蚱属应归入蚱科;在种的水平上,细庭蚱Hedotettix gracilis、粗体蚱Tetrix grossus、日本蚱Tetrix japonica种内分化明显。
In order to construct the phylogenetic relationship of Tetrigoidea and explore the differences between the molecular phylogenetic relationship and the tradition taxonomy,the COI gene sequences of 16 species of Tetrigoidea were amplified and sequenced.Furthermore,the record of NCBI was also taken into consideration.Inferred from the COI gene sequences(597bp)of the 6family,17 genera and 41 species of Tetrigoidea,the phylogenetic relationship of Tetrigoidea was reconstructed by using Neighbor Joining(NJ),Maximum Parsimony(MP),Most Likehood(ML)and Bayesian inference(BI)methods.The results indicate that Tetrigoidea is a monophyletic group.Tetrix is the most evolved group and followed by Scelimenidae,but the phylogenetic relationship of other families is not clear in Tetrigoidea.Cladonotidae,Scelimenidae,Metrodoridae,Tetrigidae are not monophyletic groups.In the category of genera,Saussurella,Eucriotettix,Thoradonta are monophyletic groups,Eucriotettixand Thoradonta are sister groups,but other genera are not monophyletic groups.Pseudepitettixof Cladonotidae should be included in Tetrigidae.In the category of species,the intraspecific differentiation of Hedotettix gracilis,Tetrix grossus and Tetrix japonicais obvious.
引文
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