蚱总科昆虫部分类群线粒体COI基因序列与系统发育关系
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摘要
为构建蚱总科昆虫的系统发育关系,探讨蚱总科传统分类和分子系统发育关系的异同,扩增并测定了16种蚱类昆虫COI基因部分序列,结合NCBI记录,对蚱总科6科17属41种蚱的COI基因597bp序列,分别采用邻接法(NJ)、最简约法(MP)、最大似然法(ML)和贝叶斯法(BI)重建蚱总科的系统发育关系。结果表明:蚱总科为一单系类群,其中蚱科较为进化,其次为刺翼蚱科,其余各科在蚱总科中的位置不确定,枝背蚱科、刺翼蚱科、短翼蚱科、蚱科均不是单系类群;在属的水平上,股沟蚱属、优角蚱属、瘤蚱属是单系类群,瘤蚱属与优角蚱属形成姐妹群,其他属不是单系类群,拟后蚱属应归入蚱科;在种的水平上,细庭蚱Hedotettix gracilis、粗体蚱Tetrix grossus、日本蚱Tetrix japonica种内分化明显。
In order to construct the phylogenetic relationship of Tetrigoidea and explore the differences between the molecular phylogenetic relationship and the tradition taxonomy,the COI gene sequences of 16 species of Tetrigoidea were amplified and sequenced.Furthermore,the record of NCBI was also taken into consideration.Inferred from the COI gene sequences(597bp)of the 6family,17 genera and 41 species of Tetrigoidea,the phylogenetic relationship of Tetrigoidea was reconstructed by using Neighbor Joining(NJ),Maximum Parsimony(MP),Most Likehood(ML)and Bayesian inference(BI)methods.The results indicate that Tetrigoidea is a monophyletic group.Tetrix is the most evolved group and followed by Scelimenidae,but the phylogenetic relationship of other families is not clear in Tetrigoidea.Cladonotidae,Scelimenidae,Metrodoridae,Tetrigidae are not monophyletic groups.In the category of genera,Saussurella,Eucriotettix,Thoradonta are monophyletic groups,Eucriotettixand Thoradonta are sister groups,but other genera are not monophyletic groups.Pseudepitettixof Cladonotidae should be included in Tetrigidae.In the category of species,the intraspecific differentiation of Hedotettix gracilis,Tetrix grossus and Tetrix japonicais obvious.
In order to construct the phylogenetic relationship of Tetrigoidea and explore the differences between the molecular phylogenetic relationship and the tradition taxonomy,the COI gene sequences of 16 species of Tetrigoidea were amplified and sequenced.Furthermore,the record of NCBI was also taken into consideration.Inferred from the COI gene sequences(597bp)of the 6family,17 genera and 41 species of Tetrigoidea,the phylogenetic relationship of Tetrigoidea was reconstructed by using Neighbor Joining(NJ),Maximum Parsimony(MP),Most Likehood(ML)and Bayesian inference(BI)methods.The results indicate that Tetrigoidea is a monophyletic group.Tetrix is the most evolved group and followed by Scelimenidae,but the phylogenetic relationship of other families is not clear in Tetrigoidea.Cladonotidae,Scelimenidae,Metrodoridae,Tetrigidae are not monophyletic groups.In the category of genera,Saussurella,Eucriotettix,Thoradonta are monophyletic groups,Eucriotettixand Thoradonta are sister groups,but other genera are not monophyletic groups.Pseudepitettixof Cladonotidae should be included in Tetrigidae.In the category of species,the intraspecific differentiation of Hedotettix gracilis,Tetrix grossus and Tetrix japonicais obvious.
引文
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[3]郑哲民.中国西部蚱总科志[M].北京:科学出版社,2005.
[4]邓维安,郑哲民,韦仕珍.滇桂地区蚱总科动物志[M].南宁:广西科学技术出版社,2007.
[5]FLOOK P K,ROWELL C H F.The phylogeny of the caelifera(Insecta,Orthoptera)as deduced from mtrRNA gene sequences[J].Mol Phylogenet Evol,1997,8(1):89-103.
[6]FLOOK P K,ROWELL C H F.The effectiveness of mitochondrial rRNA genese quences for the reconstruction of the phylogeny of an insect order(Orthoptera)[J].Mol Pylogenet Evol,1997,8(2):177-192.
[7]蒋国芳.蚱总科昆虫线粒体细胞色素b基因序列及系统进化研究[D].西安:陕西师范大学图书馆,2000.
[8]陈爱辉.中国蚱总科昆虫的系统关系研究[D].南京:南京师范大学图书馆,2005.
[9]姚艳萍.中国蚱总科部分种类16SrRNA和18SrRNA基因序列的分子进化与系统学研究[D].西安:陕西师范大学图书馆,2008.
[10]FANG N,XUAN W J,ZHANG Y Y,et al.Molecular phylogeny of Chinese tetrigoids(Othopthera,Tetrigoidea)based on the mitochondrial cytochrome coxidasen 1gene[J].Acta Zootaxonomica Sinica,2010,35(4):696-702.
[11]林立亮.三种蚱总科昆虫线粒体基因分析及系统发育关系研究[D].西安:陕西师范大学图书馆,2014.
[12]印红,刘晓丽,王彦芳,等.一种改进的昆虫基因组DNA的提取方法[J].河北大学学报:自然科学版,2002,22(1):80-82.
[13]田英芳,黄刚,郑哲民,等.一种简易的昆虫基因组DNA提取方法[J].陕西师范大学学报:自然科学版,1999,27(24):82-84.
[14]李晓东.基于线粒体基因及形态特征的竹蝗属和雷篦蝗属系统学研究[D].西安:陕西师范大学图书馆,2008.
[15]KNIGHT A,MINDELL D P.Subsititions,weighting of DNA sequence evolution and the phylogeny position of feaps viper[J].Syst Biol,1993,42(1):18-31.