响应面法优化缬草多糖的提取工艺及其抗氧化性
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摘要
采用响应面法优化缬草多糖的提取工艺,并测定其体外抗氧活性。在单因素试验基础上,考察提取温度、提取时间和料液比对缬草多糖得率的影响。结果表明:在提取时间2.6h、提取温度81℃、料液比1:27(g:m L)条件下,多糖得率最高,为17.00%,与理论值17.24%相差较小;不同浓度多糖(0.2~1 mg/m L)对DPPH(62.9%~82.5%)和羟基自由基(45.6%~80.2%)都有较好的清除效果,且在一定范围内与浓度呈正相关性。由此可知,所得回归模型可用于缬草多糖提取工艺预测,且缬草多糖在体外具有较好的抗氧化活性。
The extracting process of the polysaccharides from Valerian root was optimized by using response surface experients. At the same time, the anti-oxidant activity of polysaccharide was measured by anti-oxidant tests. Based on single factor tests, the impact of temperature, time, and ratio of materials on the yield of polysaccharides extracted from Valerian root was analyzed. The results showed that the optimazed processing is as follows, extracting time 2.6 h, solid-liquid 1:27(g:m L) and temperature 81℃. Under the optimal condition, the heighest yield of the polysaccharides was 17.00%, which was close to the theoretical value of 17.24%(Relative deviation was 0.24%). With concentrations of the Valerian polysaccharides ranging from 0.2 to 1 mg/ml, it had signifcant effect on the scavenging rate of DPPH(62.9%~82.5%)and Hydroxyl radical(45.6%~80.2%), and there was a positive correlation between the scavenging rate and the concentration of the Valerian polysaccharides in a certain range. In conclusion, it was feasible to extract valerian polysaccharides by response surface methodology, and valerian polysaccharide has good antioxidant activity in vitro.
The extracting process of the polysaccharides from Valerian root was optimized by using response surface experients. At the same time, the anti-oxidant activity of polysaccharide was measured by anti-oxidant tests. Based on single factor tests, the impact of temperature, time, and ratio of materials on the yield of polysaccharides extracted from Valerian root was analyzed. The results showed that the optimazed processing is as follows, extracting time 2.6 h, solid-liquid 1:27(g:m L) and temperature 81℃. Under the optimal condition, the heighest yield of the polysaccharides was 17.00%, which was close to the theoretical value of 17.24%(Relative deviation was 0.24%). With concentrations of the Valerian polysaccharides ranging from 0.2 to 1 mg/ml, it had signifcant effect on the scavenging rate of DPPH(62.9%~82.5%)and Hydroxyl radical(45.6%~80.2%), and there was a positive correlation between the scavenging rate and the concentration of the Valerian polysaccharides in a certain range. In conclusion, it was feasible to extract valerian polysaccharides by response surface methodology, and valerian polysaccharide has good antioxidant activity in vitro.
引文
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[16]陈仕学,郁建平,杨俊,等.响应面法优化微波提取阳荷水溶性膳食纤维工艺[J].食品科学,2014,35(18):57-62.
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[2]殷勇冠,朱全红,王彩云.缬草的研究进展[J].医药导报,2006,25(3):230-231.
[3]吴迪,张楠淇,李平亚.缬草化学成分及生物活性研究进展[J].中国中医药信息杂志,2014,21(9):129-133.
[4]HENDRIKS H,BOS R,ALLERSMA D P,et al.Pharmacolog ical screening of valerenal and some other components of essential oil of Valeriana officinalis[J].Planta Medica,1981,42(05):62-68.
[5]BOUNTHANH C,BERGMANN C,BECK J P,et al.Valepot riates,a new class of cytotoxic and antitumor agents[J].Planta Medica,1981,41(01):21-28.
[6]薛存宽,蒋彦章.神农缬草挥发油对动物心血管系统的作用[J].中西医结合杂志,1988,8(1):8-12.
[7]杨晶,薛存宽.神农缬草挥发油的平喘作用[J].中西医结合杂志,1998,18(2):28-30.
[8]俞慧红,戴飞,王巧英,等.多糖抗氧化作用的研究现状[J].食品研究与开发,2008,29(3):172-175.
[9]刘荣,韦正,银铃,等.星点设计-效应面法优化陈皮多糖提取工艺[J].中国实验方剂学杂志,2013,19(18):23-26.
[10]薛存宽,沈凯,蒋鹏,等.缬草主要活性成分的初步研究[J].现代中西医结合杂志,2014,21(9):912-914,993.
[11]纪鹏,张蔓,孙红国,等.响应面分析优化当归粗多糖提取工艺[J].天然产物研究与开发,2013,25:976-981,888.
[12]郑林龙,蒋剑平,许海顺,等.响应面法优化土茯苓多糖的提取工艺及抗氧化活性研究[J].中华中医药杂志,2014,29(3):918-922.
[13]陈仕学,沈家国,陈波,等.微波辅助提取梵净山阳荷多糖的工艺优化[J].食品与发酵工业,2013,39(5):234-237.
[14]李姣娟,黄克瀛,龚建良,等.油茶叶乙醇提取物清除DPPH自由基作用的研究[J].林产化学与工业,2008,28(2):83-84.
[15]王永宁,石玉平,郭珍.沙枣花中黄酮类化合物对羟基自由基的清除研究[J].青海医学院报,2003,24(4):281-283.
[16]陈仕学,郁建平,杨俊,等.响应面法优化微波提取阳荷水溶性膳食纤维工艺[J].食品科学,2014,35(18):57-62.
[17]GIOVINNIM.Response surface methodology and product optimization[J].Food Technology,1999,37(2):41-45.
[18]王顺民,汤斌,余建斌,等.响应面法优化菜籽皮可溶性膳食纤维提取工艺[J].中国粮油学报,2011,26(9):98-103.