仔猪肠道微生物代谢产物及益生菌的调控作用
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摘要
近年来,肠道微生物组成及其代谢产物与宿主生理之间的互作关系成为当前动物营养研究的热点。研究表明,肠道微生物的代谢产物是影响宿主生理的关键执行者,肠道微生物具有调节宿主代谢、免疫、生长发育等作用。本文系统地综述了仔猪肠道微生物组成、代谢产物种类和功能,以及益生菌对仔猪肠道微生物的影响,以期为仔猪营养调控和肠道微生物的研究提供科学依据。
In recent years,the associations between intestinal microbial composition,host diet and host physiology has increasingly attracted the attention in animal nutrition research. Gut microbiota can regulate the metabolism,immunity,growth and development of the host via its metabolites. This article systematically reviews the composition of intestinal microbiota,the types of microbiota metabolites,and the effect of probiotic supplementation on the intestinal microbiota of piglets,in order to provide some guidance for future research and nutritional regulation on the intestinal microbiota in piglets.
In recent years,the associations between intestinal microbial composition,host diet and host physiology has increasingly attracted the attention in animal nutrition research. Gut microbiota can regulate the metabolism,immunity,growth and development of the host via its metabolites. This article systematically reviews the composition of intestinal microbiota,the types of microbiota metabolites,and the effect of probiotic supplementation on the intestinal microbiota of piglets,in order to provide some guidance for future research and nutritional regulation on the intestinal microbiota in piglets.
引文
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[4]DONALDSON G P,LEE S M,MAZMANIAN S K.Gut biogeography of the bacterial microbiota[J].Nature Reviews Microbiology,2016,14(1):20-32.
[5]KELLY J,DALY K,MORAN A W,et al.Composition and diversity of mucosa-associated microbiota along the entire length of the pig gastrointestinal tract;dietary influences[J].Environmental Microbiology,2017,19(4):1425-1438.
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[7]YAN S J,HUANG J F,CHEN Z J,et al.Metabolomics in gut microbiota:applications and challenges[J].Science Bulletin,2016,61(15):1151-1153.
[8]KHATIBJOO A,MAHMOODI M,FATTAHNIA F,et al.Effects of dietary short-and medium-chain fatty acids on performance,carcass traits,jejunum morphology,and serum parameters of broiler chickens[J].Journal of Applied Animal Research,2017,46(1):492-498.
[9]OHIRA H,TSUTSUI W,FUJIOKA Y.Are short chain fatty acids in gut microbiota defensive players for inflammation and atherosclerosis?[J].Journal of Atherosclerosis and Thrombosis,2017,24(7):660-672.
[10]WANG L L,GUO H H,HUANG S,et al.Comprehensive evaluation of SCFA production in the intestinal bacteria regulated by berberine using gas-chromatography combined with polymerase chain reaction[J].Journal of Chromatography B,2017,1057:70-80.
[11]TURRONI S,BRIGIDI P,CAVALLI A,et al.Microbiota-host transgenomic metabolism,bioactive molecules from the inside[J].Journal of Medicinal Chemistry,2018,61(1):47-61.
[12]HUSTED A S,TRAUELSEN M,RUDENKO O,et al.GPCR-mediated signaling of metabolites[J].Cell Metabolism,2017,25(4):777-796.
[13]SHAPIRO H,KOLODZIEJCZYK A A,HALSTUCH D,et al.Bile acids in glucose metabolism in health and disease[J].The Journal of Experimental Medicine,2018,215(2):383-396.
[14]WAHLSTRM A,SAYIN S I,MARSCHALL H U,et al.Intestinal crosstalk between bile acids and microbiota and its impact on host metabolism[J].Cell Metabolism,2016,24(1):41-50.
[15]LONG S L,GAHAN C G M,JOYCE S A.Interactions between gut bacteria and bile in health and disease[J].Molecular Aspects of Medicine,2017,56:54-65.
[16]GHAFFARZADEGAN T,ZHONG Y D,FAKHLLENIUS F,et al.Effects of barley variety,dietary fiber andβ-glucan content on bile acid composition in cecum of rats fed low-and high-fat diets[J].The Journal of Nutritional Biochemistry,2018,53:104-110.
[17]WANG Z N,KLIPFELL E,BENNETT B J,et al.Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease[J].Nature,2011,472(7341):57-63.
[18]KOETH R A,WANG Z N,LEVISON B S,et al.Intestinal microbiota metabolism of L-carnitine,a nutrient in red meat,promotes atherosclerosis[J].Nature Medicine,2013,19(5):576-585.
[19]ZHU W F,GREGORY J C,ORG E,et al.Gut microbial metabolite TMAO enhances platelet hyperreactivity and thrombosis Risk[J].Cell,2016,165(1):111-124.
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[21]ZELANTE T,IANNITTI R G,CUNHA C,et al.Tryptophan catabolites from microbiota engage aryl hydrocarbon receptor and balance mucosal reactivity via interleukin-22[J].Immunity,2013,39(2):372-385.
[22]BANSAL T,ALANIZ R C,WOOD T K,et al.The bacterial signal indole increases epithelial-cell tight-junction resistance and attenuates indicators of inflammation[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(1):228-233.
[23]DODD D,SPITZER M H,VAN TREUREN W,et al.Agut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites[J].Nature,2017,551(7682):648-652.
[24]LIU Y H,ESPINOSA C D,ABELILLA J J,et al.Nonantibiotic feed additives in diets for pigs:a review[J].Animal Nutrition,2018,4(2):113-125.
[25]KIROS T G,DERAKHSHANI H,PINLOCHE E,et al.Effect of live yeast Saccharomyces cerevisiae(Actisaf Sc47)supplementation on the performance and hindgut microbiota composition of weanling pigs[J].Scientific Reports,2018,8(1):5315.
[26]ZHU C,WANG L,WEI S Y,et al.Effect of yeast Saccharomyces cerevisiae supplementation on serum antioxidant capacity,mucosal sIgA secretions and gut microbial populations in weaned piglets[J].Journal of Integrative Agriculture,2017,16(9):2029-2037
[27]ZHAO P Y,ZHANG Z F,LAN R X,et al.Comparison of efficacy of lactic acid bacteria complex and Enterococcus faecium DSM 7134 in weanling pigs[J].Journal of Applied Animal Research,2018,46(1):888-892.
[28]XIE Y H,ZHANG C Y,WANG L X,et al.Effects of dietary supplementation of Enterococcus faecium on growth performance,intestinal morphology,and selected microbial populations of piglets[J].Livestock Science,2018,210:111-117.
[29]LI Y,ZHANG H,SU W P,et al.Effects of dietary Bacillus amyloliquefaciens supplementation on growth performance,intestinal morphology,inflammatory response,and microbiota of intra-uterine growth retarded weanling piglets[J].Journal of Animal Science and Biotechnology,2018,9:22.
[30]BARBA-VIDAL E,CASTILLEJOS L,ROLL V F B,et al.The probiotic combination of Bifidobacterium longum subsp.infantis CECT 7210 and Bifidobacterium animalis subsp.lactis BPL6 reduces pathogen loads and improves gut health of weaned piglets orally challenged with Salmonella Typhimurium[J].Frontiers in Microbiology,2017,8:1570.
[31]SASSONE-CORSI M,NUCCIO S P,LIU P,et al.Microcins mediate competition among Enterobacteriaceae in the inflamed gut[J].Nature,2016,540(7632):280-283.
[32]KERN M,ASCHENBACH J R,TEDIN K,et al.Characterization of inflammasome components in pig intestine and analysis of the influence of probiotic Enterococcus Faecium during an Escherichia coli challenge[J].Immunological Investigations,2017,46(7):742-757.
[33]TREVISI P,CASINI L,COLORETTI F,et al.Dietary addition of Lactobacillus rhamnosus GG impairs the health of Escherichia coli F4-challenged piglets[J].Animal,2011,5(9):1354-1360.
[34]ZHANG J,CHEN X Y,LIU P,et al.Dietary Clostridium butyricum induces a phased shift in fecal microbiota structure and increases the acetic acid-producing bacteria in a weaned piglet model[J].Journal of Agricultural and Food Chemistry,2018,66(20):5157-5166.
[35]MORI K,ITO T,MIYAMOTO H,et al.Oral administration of multispecies microbial supplements to sows influences the composition of gut microbiota and fecal organic acids in their post-weaned piglets[J].Journal of Bioscience and Bioengineering,2011,112(2):145-150.
[36]YAN S J,ZHU C,YU T,et al.Studying the differences of bacterial metabolome and microbiome in the colon between Landrace and Meihua piglets[J].Frontiers in Microbiology,2017,8:1812.
[37]TOLHURST G,HEFFRON H,LAM Y S,et al.Shortchain fatty acids stimulate glucagon-like peptide-1 secretion via the G-protein-coupled receptor FFAR2[J].Diabetes,2012,61(2):364-371.
[38]YU M,MU C L,ZHANG C J,et al.Marked response in microbial community and metabolism in the ileum and cecum of suckling piglets after early antibiotics exposure[J].Frontiers in Microbiology,2018,9:1166.
[39]LU X,ZHANG M,ZHAO L,et al.Growth performance and post-weaning diarrhoea in piglets fed a diet supplemented with probiotic complexes[J].Journal of Microbiology and Biotechnology,2018,doi:10.4014/jmb.1807.07026.
[40]JIA W,XIE G X.Probiotics,bile acids and gastrointestinal carcinogenesis[J].Nature Reviews Gastroenterology&Hepatology,2018,15(4):205.
[41]JEUN J,KIM S,CHO S Y,et al.Hypocholesterolemic effects of Lactobacillus plantarum KCTC3928 by increased bile acid excretion in C57BL/6 mice[J].Nutrition,2010,26(3):321-330.
[42]PAVLOVIC'N,STANKOV K,MIKOV M.Probiotics-interactions with bile acids and impact on cholesterol metabolism[J].Applied Biochemistry and Biotechnology,2012,168(7):1880-1895.
[43]DE DIEGO-CABERO N,MEREU A,MENOYO D,et al.Bile acid mediated effects on gut integrity and performance of early-weaned piglets[J].BMC Veterinary Research,2015,11:111.
[2]GRESSE R,CHAUCHEYRAS-DURAND F,FLEURYM A,et al.Gut microbiota dysbiosis in postweaning piglets:understanding the keys to health[J].Trends in Microbiology,2017,25(10):851-873.
[3]CHEN X,XU J M,REN E D,et al.Co-occurrence of early gut colonization in neonatal piglets with microbiota in the maternal and surrounding delivery environments[J].Anaerobe,2018,49:30-40.
[4]DONALDSON G P,LEE S M,MAZMANIAN S K.Gut biogeography of the bacterial microbiota[J].Nature Reviews Microbiology,2016,14(1):20-32.
[5]KELLY J,DALY K,MORAN A W,et al.Composition and diversity of mucosa-associated microbiota along the entire length of the pig gastrointestinal tract;dietary influences[J].Environmental Microbiology,2017,19(4):1425-1438.
[6]NICHOLSON J K,HOLMES E,KINROSS J,et al.Hostgut microbiota metabolic interactions[J].Science,2012,336(6086):1262-1267.
[7]YAN S J,HUANG J F,CHEN Z J,et al.Metabolomics in gut microbiota:applications and challenges[J].Science Bulletin,2016,61(15):1151-1153.
[8]KHATIBJOO A,MAHMOODI M,FATTAHNIA F,et al.Effects of dietary short-and medium-chain fatty acids on performance,carcass traits,jejunum morphology,and serum parameters of broiler chickens[J].Journal of Applied Animal Research,2017,46(1):492-498.
[9]OHIRA H,TSUTSUI W,FUJIOKA Y.Are short chain fatty acids in gut microbiota defensive players for inflammation and atherosclerosis?[J].Journal of Atherosclerosis and Thrombosis,2017,24(7):660-672.
[10]WANG L L,GUO H H,HUANG S,et al.Comprehensive evaluation of SCFA production in the intestinal bacteria regulated by berberine using gas-chromatography combined with polymerase chain reaction[J].Journal of Chromatography B,2017,1057:70-80.
[11]TURRONI S,BRIGIDI P,CAVALLI A,et al.Microbiota-host transgenomic metabolism,bioactive molecules from the inside[J].Journal of Medicinal Chemistry,2018,61(1):47-61.
[12]HUSTED A S,TRAUELSEN M,RUDENKO O,et al.GPCR-mediated signaling of metabolites[J].Cell Metabolism,2017,25(4):777-796.
[13]SHAPIRO H,KOLODZIEJCZYK A A,HALSTUCH D,et al.Bile acids in glucose metabolism in health and disease[J].The Journal of Experimental Medicine,2018,215(2):383-396.
[14]WAHLSTRM A,SAYIN S I,MARSCHALL H U,et al.Intestinal crosstalk between bile acids and microbiota and its impact on host metabolism[J].Cell Metabolism,2016,24(1):41-50.
[15]LONG S L,GAHAN C G M,JOYCE S A.Interactions between gut bacteria and bile in health and disease[J].Molecular Aspects of Medicine,2017,56:54-65.
[16]GHAFFARZADEGAN T,ZHONG Y D,FAKHLLENIUS F,et al.Effects of barley variety,dietary fiber andβ-glucan content on bile acid composition in cecum of rats fed low-and high-fat diets[J].The Journal of Nutritional Biochemistry,2018,53:104-110.
[17]WANG Z N,KLIPFELL E,BENNETT B J,et al.Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease[J].Nature,2011,472(7341):57-63.
[18]KOETH R A,WANG Z N,LEVISON B S,et al.Intestinal microbiota metabolism of L-carnitine,a nutrient in red meat,promotes atherosclerosis[J].Nature Medicine,2013,19(5):576-585.
[19]ZHU W F,GREGORY J C,ORG E,et al.Gut microbial metabolite TMAO enhances platelet hyperreactivity and thrombosis Risk[J].Cell,2016,165(1):111-124.
[20]GAO J,XU K,LIU H N,et al.Impact of the gut microbiota on intestinal immunity mediated by tryptophan metabolism[J].Frontiers in Cellular and Infection Microbiology,2018,8:13.
[21]ZELANTE T,IANNITTI R G,CUNHA C,et al.Tryptophan catabolites from microbiota engage aryl hydrocarbon receptor and balance mucosal reactivity via interleukin-22[J].Immunity,2013,39(2):372-385.
[22]BANSAL T,ALANIZ R C,WOOD T K,et al.The bacterial signal indole increases epithelial-cell tight-junction resistance and attenuates indicators of inflammation[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(1):228-233.
[23]DODD D,SPITZER M H,VAN TREUREN W,et al.Agut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites[J].Nature,2017,551(7682):648-652.
[24]LIU Y H,ESPINOSA C D,ABELILLA J J,et al.Nonantibiotic feed additives in diets for pigs:a review[J].Animal Nutrition,2018,4(2):113-125.
[25]KIROS T G,DERAKHSHANI H,PINLOCHE E,et al.Effect of live yeast Saccharomyces cerevisiae(Actisaf Sc47)supplementation on the performance and hindgut microbiota composition of weanling pigs[J].Scientific Reports,2018,8(1):5315.
[26]ZHU C,WANG L,WEI S Y,et al.Effect of yeast Saccharomyces cerevisiae supplementation on serum antioxidant capacity,mucosal sIgA secretions and gut microbial populations in weaned piglets[J].Journal of Integrative Agriculture,2017,16(9):2029-2037
[27]ZHAO P Y,ZHANG Z F,LAN R X,et al.Comparison of efficacy of lactic acid bacteria complex and Enterococcus faecium DSM 7134 in weanling pigs[J].Journal of Applied Animal Research,2018,46(1):888-892.
[28]XIE Y H,ZHANG C Y,WANG L X,et al.Effects of dietary supplementation of Enterococcus faecium on growth performance,intestinal morphology,and selected microbial populations of piglets[J].Livestock Science,2018,210:111-117.
[29]LI Y,ZHANG H,SU W P,et al.Effects of dietary Bacillus amyloliquefaciens supplementation on growth performance,intestinal morphology,inflammatory response,and microbiota of intra-uterine growth retarded weanling piglets[J].Journal of Animal Science and Biotechnology,2018,9:22.
[30]BARBA-VIDAL E,CASTILLEJOS L,ROLL V F B,et al.The probiotic combination of Bifidobacterium longum subsp.infantis CECT 7210 and Bifidobacterium animalis subsp.lactis BPL6 reduces pathogen loads and improves gut health of weaned piglets orally challenged with Salmonella Typhimurium[J].Frontiers in Microbiology,2017,8:1570.
[31]SASSONE-CORSI M,NUCCIO S P,LIU P,et al.Microcins mediate competition among Enterobacteriaceae in the inflamed gut[J].Nature,2016,540(7632):280-283.
[32]KERN M,ASCHENBACH J R,TEDIN K,et al.Characterization of inflammasome components in pig intestine and analysis of the influence of probiotic Enterococcus Faecium during an Escherichia coli challenge[J].Immunological Investigations,2017,46(7):742-757.
[33]TREVISI P,CASINI L,COLORETTI F,et al.Dietary addition of Lactobacillus rhamnosus GG impairs the health of Escherichia coli F4-challenged piglets[J].Animal,2011,5(9):1354-1360.
[34]ZHANG J,CHEN X Y,LIU P,et al.Dietary Clostridium butyricum induces a phased shift in fecal microbiota structure and increases the acetic acid-producing bacteria in a weaned piglet model[J].Journal of Agricultural and Food Chemistry,2018,66(20):5157-5166.
[35]MORI K,ITO T,MIYAMOTO H,et al.Oral administration of multispecies microbial supplements to sows influences the composition of gut microbiota and fecal organic acids in their post-weaned piglets[J].Journal of Bioscience and Bioengineering,2011,112(2):145-150.
[36]YAN S J,ZHU C,YU T,et al.Studying the differences of bacterial metabolome and microbiome in the colon between Landrace and Meihua piglets[J].Frontiers in Microbiology,2017,8:1812.
[37]TOLHURST G,HEFFRON H,LAM Y S,et al.Shortchain fatty acids stimulate glucagon-like peptide-1 secretion via the G-protein-coupled receptor FFAR2[J].Diabetes,2012,61(2):364-371.
[38]YU M,MU C L,ZHANG C J,et al.Marked response in microbial community and metabolism in the ileum and cecum of suckling piglets after early antibiotics exposure[J].Frontiers in Microbiology,2018,9:1166.
[39]LU X,ZHANG M,ZHAO L,et al.Growth performance and post-weaning diarrhoea in piglets fed a diet supplemented with probiotic complexes[J].Journal of Microbiology and Biotechnology,2018,doi:10.4014/jmb.1807.07026.
[40]JIA W,XIE G X.Probiotics,bile acids and gastrointestinal carcinogenesis[J].Nature Reviews Gastroenterology&Hepatology,2018,15(4):205.
[41]JEUN J,KIM S,CHO S Y,et al.Hypocholesterolemic effects of Lactobacillus plantarum KCTC3928 by increased bile acid excretion in C57BL/6 mice[J].Nutrition,2010,26(3):321-330.
[42]PAVLOVIC'N,STANKOV K,MIKOV M.Probiotics-interactions with bile acids and impact on cholesterol metabolism[J].Applied Biochemistry and Biotechnology,2012,168(7):1880-1895.
[43]DE DIEGO-CABERO N,MEREU A,MENOYO D,et al.Bile acid mediated effects on gut integrity and performance of early-weaned piglets[J].BMC Veterinary Research,2015,11:111.